Organization and regulation of the arsenite oxidase operon of the moderately acidophilic and facultative chemoautotrophic Thiomonas arsenitoxydans
Thiomonas arsenitoxydans is an acidophilic and facultatively autotrophic bacterium that can grow by oxidizing arsenite to arsenate. A comparative genomic analysis showed that the T. arsenitoxydans aioBA cluster encoding the two subunits of arsenite oxidase is distinct from the other clusters, with t...
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| Veröffentlicht in: | Extremophiles : life under extreme conditions 2013-11, Vol.17 (6), p.911-920 |
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| creator | Slyemi, Djamila Moinier, Danielle Talla, Emmanuel Bonnefoy, Violaine |
| description | Thiomonas arsenitoxydans
is an acidophilic and facultatively autotrophic bacterium that can grow by oxidizing arsenite to arsenate. A comparative genomic analysis showed that the
T. arsenitoxydans
aioBA
cluster encoding the two subunits of arsenite oxidase is distinct from the other clusters, with two specific genes encoding a cytochrome
c
and a metalloregulator belonging to the ArsR/SmtB family. These genes are cotranscribed with
aioBA
, suggesting that these cytochromes
c
are involved in arsenite oxidation and that this operon is controlled by the metalloregulator. The growth of
T. arsenitoxydans
in the presence of thiosulfate and arsenite, or arsenate, is biphasic. Real-time PCR experiments showed that the operon is transcribed during the second growth phase in the presence of arsenite or arsenate, whereas antimonite had no effect. These results suggest that the expression of the
aioBA
operon of
T. arsenitoxydans
is regulated by the electron donor present in the medium, i.e., is induced in the presence of arsenic but is repressed by more energetic substrates. Our data indicate that the genetic organization and regulation of the
aioBA
operon of
T. arsenitoxydans
differ from those of the other arsenite oxidizers. |
| doi_str_mv | 10.1007/s00792-013-0573-1 |
| format | Article |
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is an acidophilic and facultatively autotrophic bacterium that can grow by oxidizing arsenite to arsenate. A comparative genomic analysis showed that the
T. arsenitoxydans
aioBA
cluster encoding the two subunits of arsenite oxidase is distinct from the other clusters, with two specific genes encoding a cytochrome
c
and a metalloregulator belonging to the ArsR/SmtB family. These genes are cotranscribed with
aioBA
, suggesting that these cytochromes
c
are involved in arsenite oxidation and that this operon is controlled by the metalloregulator. The growth of
T. arsenitoxydans
in the presence of thiosulfate and arsenite, or arsenate, is biphasic. Real-time PCR experiments showed that the operon is transcribed during the second growth phase in the presence of arsenite or arsenate, whereas antimonite had no effect. These results suggest that the expression of the
aioBA
operon of
T. arsenitoxydans
is regulated by the electron donor present in the medium, i.e., is induced in the presence of arsenic but is repressed by more energetic substrates. Our data indicate that the genetic organization and regulation of the
aioBA
operon of
T. arsenitoxydans
differ from those of the other arsenite oxidizers.</description><identifier>ISSN: 1431-0651</identifier><identifier>EISSN: 1433-4909</identifier><identifier>DOI: 10.1007/s00792-013-0573-1</identifier><identifier>PMID: 23974983</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Antimony - metabolism ; Arsenates - metabolism ; Arsenic ; Arsenic - metabolism ; Arsenites - metabolism ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Betaproteobacteria - enzymology ; Betaproteobacteria - genetics ; Betaproteobacteria - metabolism ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Enzymes ; Genes, Bacterial ; Genetics ; Life Sciences ; Microbial Ecology ; Microbiology ; Microbiology and Parasitology ; Molecular Sequence Data ; Operon ; Original Paper ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Space life sciences</subject><ispartof>Extremophiles : life under extreme conditions, 2013-11, Vol.17 (6), p.911-920</ispartof><rights>Springer Japan 2013</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-2722a9c3436b5c304acfe4dbfeb2abce6cea061b7eb9c8318bd5adac28a290633</citedby><cites>FETCH-LOGICAL-c439t-2722a9c3436b5c304acfe4dbfeb2abce6cea061b7eb9c8318bd5adac28a290633</cites><orcidid>0000-0002-6008-8476 ; 0000-0002-7775-8296</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00792-013-0573-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00792-013-0573-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23974983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04577859$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Slyemi, Djamila</creatorcontrib><creatorcontrib>Moinier, Danielle</creatorcontrib><creatorcontrib>Talla, Emmanuel</creatorcontrib><creatorcontrib>Bonnefoy, Violaine</creatorcontrib><title>Organization and regulation of the arsenite oxidase operon of the moderately acidophilic and facultative chemoautotrophic Thiomonas arsenitoxydans</title><title>Extremophiles : life under extreme conditions</title><addtitle>Extremophiles</addtitle><addtitle>Extremophiles</addtitle><description>Thiomonas arsenitoxydans
is an acidophilic and facultatively autotrophic bacterium that can grow by oxidizing arsenite to arsenate. A comparative genomic analysis showed that the
T. arsenitoxydans
aioBA
cluster encoding the two subunits of arsenite oxidase is distinct from the other clusters, with two specific genes encoding a cytochrome
c
and a metalloregulator belonging to the ArsR/SmtB family. These genes are cotranscribed with
aioBA
, suggesting that these cytochromes
c
are involved in arsenite oxidation and that this operon is controlled by the metalloregulator. The growth of
T. arsenitoxydans
in the presence of thiosulfate and arsenite, or arsenate, is biphasic. Real-time PCR experiments showed that the operon is transcribed during the second growth phase in the presence of arsenite or arsenate, whereas antimonite had no effect. These results suggest that the expression of the
aioBA
operon of
T. arsenitoxydans
is regulated by the electron donor present in the medium, i.e., is induced in the presence of arsenic but is repressed by more energetic substrates. Our data indicate that the genetic organization and regulation of the
aioBA
operon of
T. arsenitoxydans
differ from those of the other arsenite oxidizers.</description><subject>Antimony - metabolism</subject><subject>Arsenates - metabolism</subject><subject>Arsenic</subject><subject>Arsenic - metabolism</subject><subject>Arsenites - metabolism</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Betaproteobacteria - enzymology</subject><subject>Betaproteobacteria - genetics</subject><subject>Betaproteobacteria - metabolism</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Enzymes</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Life Sciences</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Molecular Sequence Data</subject><subject>Operon</subject><subject>Original Paper</subject><subject>Oxidoreductases - 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Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Extremophiles : life under extreme conditions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slyemi, Djamila</au><au>Moinier, Danielle</au><au>Talla, Emmanuel</au><au>Bonnefoy, Violaine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organization and regulation of the arsenite oxidase operon of the moderately acidophilic and facultative chemoautotrophic Thiomonas arsenitoxydans</atitle><jtitle>Extremophiles : life under extreme conditions</jtitle><stitle>Extremophiles</stitle><addtitle>Extremophiles</addtitle><date>2013-11-01</date><risdate>2013</risdate><volume>17</volume><issue>6</issue><spage>911</spage><epage>920</epage><pages>911-920</pages><issn>1431-0651</issn><eissn>1433-4909</eissn><abstract>Thiomonas arsenitoxydans
is an acidophilic and facultatively autotrophic bacterium that can grow by oxidizing arsenite to arsenate. A comparative genomic analysis showed that the
T. arsenitoxydans
aioBA
cluster encoding the two subunits of arsenite oxidase is distinct from the other clusters, with two specific genes encoding a cytochrome
c
and a metalloregulator belonging to the ArsR/SmtB family. These genes are cotranscribed with
aioBA
, suggesting that these cytochromes
c
are involved in arsenite oxidation and that this operon is controlled by the metalloregulator. The growth of
T. arsenitoxydans
in the presence of thiosulfate and arsenite, or arsenate, is biphasic. Real-time PCR experiments showed that the operon is transcribed during the second growth phase in the presence of arsenite or arsenate, whereas antimonite had no effect. These results suggest that the expression of the
aioBA
operon of
T. arsenitoxydans
is regulated by the electron donor present in the medium, i.e., is induced in the presence of arsenic but is repressed by more energetic substrates. Our data indicate that the genetic organization and regulation of the
aioBA
operon of
T. arsenitoxydans
differ from those of the other arsenite oxidizers.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><pmid>23974983</pmid><doi>10.1007/s00792-013-0573-1</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-6008-8476</orcidid><orcidid>https://orcid.org/0000-0002-7775-8296</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1431-0651 |
| ispartof | Extremophiles : life under extreme conditions, 2013-11, Vol.17 (6), p.911-920 |
| issn | 1431-0651 1433-4909 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_04577859v1 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Antimony - metabolism Arsenates - metabolism Arsenic Arsenic - metabolism Arsenites - metabolism Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Base Sequence Betaproteobacteria - enzymology Betaproteobacteria - genetics Betaproteobacteria - metabolism Biochemistry Biomedical and Life Sciences Biotechnology Enzymes Genes, Bacterial Genetics Life Sciences Microbial Ecology Microbiology Microbiology and Parasitology Molecular Sequence Data Operon Original Paper Oxidoreductases - genetics Oxidoreductases - metabolism Space life sciences |
| title | Organization and regulation of the arsenite oxidase operon of the moderately acidophilic and facultative chemoautotrophic Thiomonas arsenitoxydans |
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