An international inter-laboratory study to compare digital PCR with ISO standardized qPCR assays for the detection of norovirus GI and GII in oyster tissue

An optimized digital RT-PCR (RT-dPCR) assay for the detection of human norovirus GI and GII RNA was compared with ISO 15216-conform quantitative real-time RT-PCR (RT-qPCR) assays in an interlaboratory study (ILS) among eight laboratories. A duplex GI/GII RT-dPCR assay, based on the ISO 15216-oligonu...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Food microbiology 2024-06, Vol.120, p.104478-104478, Article 104478
Hauptverfasser:	Boxman, Ingeborg L.A., Molin, Ramia, Persson, Sofia, Juréus, Anna, Jansen, Claudia C.C., Sosef, Nils P., Le Guyader, Soizick F., Ollivier, Joanna, Summa, Maija, Hautaniemi, Maria, Suffredini, Elisabetta, Di Pasquale, Simona, Myrmel, Mette, Khatri, Mamata, Jamnikar-Ciglenecki, Urska, Kusar, Darja, Moor, Dominik, Butticaz, Lisa, Lowther, James A., Walker, David I., Stapleton, Tina, Simonsson, Magnus, Dirks, René A.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Digital PCR DNA Duplex food microbiology Food safety Humans Life Sciences Norovirus Norovirus - genetics Ostreidae Oyster oysters Quantification Real-Time Polymerase Chain Reaction - methods RNA RNA, Viral - genetics Seafood - analysis
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!