Morphological, molecular, and MALDI-TOF MS identification of bed bugs and associated Wolbachia species from Cameroon
•This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus in Cameroon.•Of the 248 bed bug specimens, 246 (77 males, 65 females and 104 immature specimens) were submitted to MALDI-TOF MS analysis.•MALDI-TOF MS has shown its effectiveness in identifying beb bugs...
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| Veröffentlicht in: | Acta tropica 2024-01, Vol.249, p.107086-107086, Article 107086 |
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| creator | Ngnindji-Youdje, Yannick Lontsi-Demano, Michel Diarra, Adama Zan Makaila, Ahmat Mahamat Tchuinkam, Timoléon Berenger, Jean-Michel Parola, Philippe |
| description | •This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus in Cameroon.•Of the 248 bed bug specimens, 246 (77 males, 65 females and 104 immature specimens) were submitted to MALDI-TOF MS analysis.•MALDI-TOF MS has shown its effectiveness in identifying beb bugs.•More surveys in the country are warranted to assess the true level of bed bug infestations.
After vanishing from the public eye for more than 50 years, bed bugs have resurged to become one of the most widely discussed and heavily researched insect pests in the world. This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus (Hemiptera: Cimicidae), in Cameroon. A total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in Yaoundé and Douala, Cameroon. The ability of MALDI-TOF MS to identify bed bugs was tested using heads for adults and cephalothoraxes for immature stages. Microorganism screening was performed by qPCR and confirmed by regular PCR and sequencing. Based on morphometrical criteria, four stages of immature bed bugs are represented. Of the 248 bed bug specimens morphologically identified as Cimex hemipterus, 246 (77 males, 65 females and 104 immature specimens) were submitted to MALDI-TOF MS analysis. Of the 222 adults and immature specimens tested, 122 (59.9 %) produced good quality MALDI-TOF MS spectra (35 adults and 87 immature specimens). Blind testing allowed species level identification of 98.21 % of adult and immature C. hemipterus. Among the bacteria tested, only Wolbachia DNA was found in 12/246 (4.8 %) bed bugs. More surveys in the country are warranted to assess the true level of bed bug infestations, in order to take appropriate action for their control.
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| doi_str_mv | 10.1016/j.actatropica.2023.107086 |
| format | Article |
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After vanishing from the public eye for more than 50 years, bed bugs have resurged to become one of the most widely discussed and heavily researched insect pests in the world. This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus (Hemiptera: Cimicidae), in Cameroon. A total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in Yaoundé and Douala, Cameroon. The ability of MALDI-TOF MS to identify bed bugs was tested using heads for adults and cephalothoraxes for immature stages. Microorganism screening was performed by qPCR and confirmed by regular PCR and sequencing. Based on morphometrical criteria, four stages of immature bed bugs are represented. Of the 248 bed bug specimens morphologically identified as Cimex hemipterus, 246 (77 males, 65 females and 104 immature specimens) were submitted to MALDI-TOF MS analysis. Of the 222 adults and immature specimens tested, 122 (59.9 %) produced good quality MALDI-TOF MS spectra (35 adults and 87 immature specimens). Blind testing allowed species level identification of 98.21 % of adult and immature C. hemipterus. Among the bacteria tested, only Wolbachia DNA was found in 12/246 (4.8 %) bed bugs. More surveys in the country are warranted to assess the true level of bed bug infestations, in order to take appropriate action for their control.
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After vanishing from the public eye for more than 50 years, bed bugs have resurged to become one of the most widely discussed and heavily researched insect pests in the world. This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus (Hemiptera: Cimicidae), in Cameroon. A total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in Yaoundé and Douala, Cameroon. The ability of MALDI-TOF MS to identify bed bugs was tested using heads for adults and cephalothoraxes for immature stages. Microorganism screening was performed by qPCR and confirmed by regular PCR and sequencing. Based on morphometrical criteria, four stages of immature bed bugs are represented. Of the 248 bed bug specimens morphologically identified as Cimex hemipterus, 246 (77 males, 65 females and 104 immature specimens) were submitted to MALDI-TOF MS analysis. Of the 222 adults and immature specimens tested, 122 (59.9 %) produced good quality MALDI-TOF MS spectra (35 adults and 87 immature specimens). Blind testing allowed species level identification of 98.21 % of adult and immature C. hemipterus. Among the bacteria tested, only Wolbachia DNA was found in 12/246 (4.8 %) bed bugs. More surveys in the country are warranted to assess the true level of bed bug infestations, in order to take appropriate action for their control.
[Display omitted]</description><subject>Animals</subject><subject>Bacteriology</subject><subject>Bed bugs</subject><subject>Bedbugs - genetics</subject><subject>Bedbugs - microbiology</subject><subject>Cameroon</subject><subject>Cardiology and cardiovascular system</subject><subject>Cimex hemipterus</subject><subject>Ectoparasitic Infestations</subject><subject>Emerging diseases</subject><subject>Female</subject><subject>Human health and pathology</subject><subject>Infectious diseases</subject><subject>Life Sciences</subject><subject>MALDI-TOF MS</subject><subject>Male</subject><subject>Microbiology and Parasitology</subject><subject>Parasitology</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Virology</subject><subject>Wolbachia</subject><subject>Wolbachia - genetics</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EokvhLyBzA6nZepK14xxXC6WVdtVDi9qb5TjjrldJHOykEv8eL2mrHjmN5ul7b6R5hHwBtgQG4vyw1GbUY_CDM3qZs7xIesmkeEMWIMsiEzlfvSULxhhkJRP3J-RDjIe05SXP35OTQrJCJNuCjDsfhr1v_UOKas9o51s0U6vDGdV9Q3fr7fer7Pb6gu5uqGuwH51N4Oh8T72lNTa0nh7iP1bH6I3TY9LufFtrs3eaxgGNw0ht8B3d6A6D9_1H8s7qNuKnp3lKfl38uN1cZtvrn1eb9TYzK4AxE0yvNM8rawuwvJSlbIwE4LJABF6xusl1CZIzy1YcpAABpmJWWM2kQSOLU_Jtzt3rVg3BdTr8UV47dbneqqOWfKySuXiExH6d2SH43xPGUXUuGmxb3aOfosplJSQwDjyh1Yya4GMMaF-ygaljQeqgXhWkjgWpuaDk_fx0Zqo7bF6cz40kYDMDmB7z6DComB7YG2xcQDOqxrv_OPMXmb2l4g</recordid><startdate>202401</startdate><enddate>202401</enddate><creator>Ngnindji-Youdje, Yannick</creator><creator>Lontsi-Demano, Michel</creator><creator>Diarra, Adama Zan</creator><creator>Makaila, Ahmat Mahamat</creator><creator>Tchuinkam, Timoléon</creator><creator>Berenger, Jean-Michel</creator><creator>Parola, Philippe</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-2765-3349</orcidid><orcidid>https://orcid.org/0000-0002-8497-2792</orcidid></search><sort><creationdate>202401</creationdate><title>Morphological, molecular, and MALDI-TOF MS identification of bed bugs and associated Wolbachia species from Cameroon</title><author>Ngnindji-Youdje, Yannick ; 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After vanishing from the public eye for more than 50 years, bed bugs have resurged to become one of the most widely discussed and heavily researched insect pests in the world. This study presents the basic information of infestations of tropical bed bugs, Cimex hemipterus (Hemiptera: Cimicidae), in Cameroon. A total of 248 immature stage and adult bed bug specimens were collected from households and a travel agency in Yaoundé and Douala, Cameroon. The ability of MALDI-TOF MS to identify bed bugs was tested using heads for adults and cephalothoraxes for immature stages. Microorganism screening was performed by qPCR and confirmed by regular PCR and sequencing. Based on morphometrical criteria, four stages of immature bed bugs are represented. Of the 248 bed bug specimens morphologically identified as Cimex hemipterus, 246 (77 males, 65 females and 104 immature specimens) were submitted to MALDI-TOF MS analysis. Of the 222 adults and immature specimens tested, 122 (59.9 %) produced good quality MALDI-TOF MS spectra (35 adults and 87 immature specimens). Blind testing allowed species level identification of 98.21 % of adult and immature C. hemipterus. Among the bacteria tested, only Wolbachia DNA was found in 12/246 (4.8 %) bed bugs. More surveys in the country are warranted to assess the true level of bed bug infestations, in order to take appropriate action for their control.
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| ispartof | Acta tropica, 2024-01, Vol.249, p.107086-107086, Article 107086 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacteriology Bed bugs Bedbugs - genetics Bedbugs - microbiology Cameroon Cardiology and cardiovascular system Cimex hemipterus Ectoparasitic Infestations Emerging diseases Female Human health and pathology Infectious diseases Life Sciences MALDI-TOF MS Male Microbiology and Parasitology Parasitology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Virology Wolbachia Wolbachia - genetics |
| title | Morphological, molecular, and MALDI-TOF MS identification of bed bugs and associated Wolbachia species from Cameroon |
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