Analysis of Ecstasy (MDMA)‐induced transcriptional responses in the rat cortex
ABSTRACT 3,4‐Methylenedioxymethamphetamine (MDMA, ecstasy) is a popular drug of abuse. MDMA is pharmacologically classified as an entactogen because of its affinities to classical hallucinogens and stimulants. Oral ingestion of a single dose of the drug is associated with euphoria, elevated self‐con...
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| Veröffentlicht in: | The FASEB journal 2002-12, Vol.16 (14), p.1887-1894 |
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| container_issue | 14 |
| container_start_page | 1887 |
| container_title | The FASEB journal |
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| creator | Thiriet, Nathalie Ladenheim, Bruce McCoy, Michael T. Lud Cadet, Jean |
| description | ABSTRACT
3,4‐Methylenedioxymethamphetamine (MDMA, ecstasy) is a popular drug of abuse. MDMA is pharmacologically classified as an entactogen because of its affinities to classical hallucinogens and stimulants. Oral ingestion of a single dose of the drug is associated with euphoria, elevated self‐confidence, and heightened sensory awareness in humans. Evidence for neurotoxicity in the human serotonin (5‐HT) system has been provided. In rats, a single injection of MDMA induces hyperthermia and formation of reactive oxygen species. These effects may cause MDMA‐associated, long‐term 5‐HT depletion, with the cortex being quite sensitive to the biochemical effects of MDMA. It has been suggested that these MDMA effects may be associated with molecular changes in this brain region. To test these ideas, we have made use of the cDNA array analysis, which can provide a more global view of the molecular changes secondary to MDMA injections. Our results show that the genes regulated by MDMA encode proteins that belong to signaling pathways, transcription regulators, or xenobiotic metabolism. Our observations indicate that cortical cells respond to the acute administration of MDMA by modulating transcription of several genes that might lead to long‐term changes in the brain.—Thiriet, N., Ladenheim, B., McCoy, M. T., Cadet, J. L. Analysis of Ecstasy (MDMA) ‐induced transcriptional responses in the rat cortex. FASEB J. 16, 1887–1894 (2002) |
| doi_str_mv | 10.1096/fj.02-0502com |
| format | Article |
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3,4‐Methylenedioxymethamphetamine (MDMA, ecstasy) is a popular drug of abuse. MDMA is pharmacologically classified as an entactogen because of its affinities to classical hallucinogens and stimulants. Oral ingestion of a single dose of the drug is associated with euphoria, elevated self‐confidence, and heightened sensory awareness in humans. Evidence for neurotoxicity in the human serotonin (5‐HT) system has been provided. In rats, a single injection of MDMA induces hyperthermia and formation of reactive oxygen species. These effects may cause MDMA‐associated, long‐term 5‐HT depletion, with the cortex being quite sensitive to the biochemical effects of MDMA. It has been suggested that these MDMA effects may be associated with molecular changes in this brain region. To test these ideas, we have made use of the cDNA array analysis, which can provide a more global view of the molecular changes secondary to MDMA injections. Our results show that the genes regulated by MDMA encode proteins that belong to signaling pathways, transcription regulators, or xenobiotic metabolism. Our observations indicate that cortical cells respond to the acute administration of MDMA by modulating transcription of several genes that might lead to long‐term changes in the brain.—Thiriet, N., Ladenheim, B., McCoy, M. T., Cadet, J. L. Analysis of Ecstasy (MDMA) ‐induced transcriptional responses in the rat cortex. FASEB J. 16, 1887–1894 (2002)</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.02-0502com</identifier><identifier>PMID: 12468453</identifier><language>eng</language><publisher>United States: Federation of American Society of Experimental Biology</publisher><subject>Animals ; cDNA arrays ; Cerebral Cortex - metabolism ; cortex ; Cytoskeletal Proteins - biosynthesis ; Cytoskeletal Proteins - genetics ; Extracellular Matrix Proteins - biosynthesis ; Extracellular Matrix Proteins - genetics ; gene expression ; Gene Expression Profiling ; Gene Expression Regulation - drug effects ; Life Sciences ; MDMA ; N-Methyl-3,4-methylenedioxyamphetamine - pharmacology ; Oligonucleotide Array Sequence Analysis ; Polymerase Chain Reaction ; Rats ; Rats, Sprague-Dawley ; Receptors, Serotonin - biosynthesis ; Receptors, Serotonin - genetics ; RNA, Messenger - biosynthesis ; Signal Transduction - drug effects ; SYBR green PCR ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Transcription, Genetic - drug effects</subject><ispartof>The FASEB journal, 2002-12, Vol.16 (14), p.1887-1894</ispartof><rights>FASEB</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437M-d97ea0c4add68bd49db36d205d7427342b37290b6abe858b4b7718b6f1f89663</citedby><cites>FETCH-LOGICAL-c437M-d97ea0c4add68bd49db36d205d7427342b37290b6abe858b4b7718b6f1f89663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1096%2Ffj.02-0502com$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1096%2Ffj.02-0502com$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12468453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04393505$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Thiriet, Nathalie</creatorcontrib><creatorcontrib>Ladenheim, Bruce</creatorcontrib><creatorcontrib>McCoy, Michael T.</creatorcontrib><creatorcontrib>Lud Cadet, Jean</creatorcontrib><title>Analysis of Ecstasy (MDMA)‐induced transcriptional responses in the rat cortex</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>ABSTRACT
3,4‐Methylenedioxymethamphetamine (MDMA, ecstasy) is a popular drug of abuse. MDMA is pharmacologically classified as an entactogen because of its affinities to classical hallucinogens and stimulants. Oral ingestion of a single dose of the drug is associated with euphoria, elevated self‐confidence, and heightened sensory awareness in humans. Evidence for neurotoxicity in the human serotonin (5‐HT) system has been provided. In rats, a single injection of MDMA induces hyperthermia and formation of reactive oxygen species. These effects may cause MDMA‐associated, long‐term 5‐HT depletion, with the cortex being quite sensitive to the biochemical effects of MDMA. It has been suggested that these MDMA effects may be associated with molecular changes in this brain region. To test these ideas, we have made use of the cDNA array analysis, which can provide a more global view of the molecular changes secondary to MDMA injections. Our results show that the genes regulated by MDMA encode proteins that belong to signaling pathways, transcription regulators, or xenobiotic metabolism. Our observations indicate that cortical cells respond to the acute administration of MDMA by modulating transcription of several genes that might lead to long‐term changes in the brain.—Thiriet, N., Ladenheim, B., McCoy, M. T., Cadet, J. L. Analysis of Ecstasy (MDMA) ‐induced transcriptional responses in the rat cortex. FASEB J. 16, 1887–1894 (2002)</description><subject>Animals</subject><subject>cDNA arrays</subject><subject>Cerebral Cortex - metabolism</subject><subject>cortex</subject><subject>Cytoskeletal Proteins - biosynthesis</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Extracellular Matrix Proteins - biosynthesis</subject><subject>Extracellular Matrix Proteins - genetics</subject><subject>gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Life Sciences</subject><subject>MDMA</subject><subject>N-Methyl-3,4-methylenedioxyamphetamine - pharmacology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Serotonin - biosynthesis</subject><subject>Receptors, Serotonin - genetics</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Signal Transduction - drug effects</subject><subject>SYBR green PCR</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic - drug effects</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90MtOGzEYhmGrKioBuuy28qqCxcDvw3g83YVAoFUiKpW95dMIR5Nxak-A7LgErpErYaJE7a4ry9ajV_KH0BcC5wRqcdEszoEWUAK1cfkBjUjJoBBSwEc0AlnTQggmD9FRzgsAIEDEJ3RIKBeSl2yEfo073W5yyDg2-NrmXucNPp1fzcdnby-voXNr6x3uk-6yTWHVhzh4nHxexS77jEOH-wePk-6xjan3zyfooNFt9p_35zG6n17fT26L2d3Nj8l4VljOqnnh6sprsFw7J6RxvHaGCUehdBWnFePUsIrWYIQ2XpbScFNVRBrRkEbWw5eO0dku-6BbtUphqdNGRR3U7Ximtm_AWc1KKB_JYL_t7CrFP2ufe7UM2fq21Z2P66yIlEQKuo0WO2hTzDn55m-ZgNqurZqFAqr2aw_-6z68Nkvv_un9vAP4vgNPofWb_9fU9Pclnf4Eur1P7ubsHWe3jSk</recordid><startdate>200212</startdate><enddate>200212</enddate><creator>Thiriet, Nathalie</creator><creator>Ladenheim, Bruce</creator><creator>McCoy, Michael T.</creator><creator>Lud Cadet, Jean</creator><general>Federation of American Society of Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>1XC</scope></search><sort><creationdate>200212</creationdate><title>Analysis of Ecstasy (MDMA)‐induced transcriptional responses in the rat cortex</title><author>Thiriet, Nathalie ; Ladenheim, Bruce ; McCoy, Michael T. ; Lud Cadet, Jean</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437M-d97ea0c4add68bd49db36d205d7427342b37290b6abe858b4b7718b6f1f89663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>cDNA arrays</topic><topic>Cerebral Cortex - metabolism</topic><topic>cortex</topic><topic>Cytoskeletal Proteins - biosynthesis</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Extracellular Matrix Proteins - biosynthesis</topic><topic>Extracellular Matrix Proteins - genetics</topic><topic>gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Life Sciences</topic><topic>MDMA</topic><topic>N-Methyl-3,4-methylenedioxyamphetamine - pharmacology</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Serotonin - biosynthesis</topic><topic>Receptors, Serotonin - genetics</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Signal Transduction - drug effects</topic><topic>SYBR green PCR</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - genetics</topic><topic>Transcription, Genetic - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thiriet, Nathalie</creatorcontrib><creatorcontrib>Ladenheim, Bruce</creatorcontrib><creatorcontrib>McCoy, Michael T.</creatorcontrib><creatorcontrib>Lud Cadet, Jean</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thiriet, Nathalie</au><au>Ladenheim, Bruce</au><au>McCoy, Michael T.</au><au>Lud Cadet, Jean</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Ecstasy (MDMA)‐induced transcriptional responses in the rat cortex</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2002-12</date><risdate>2002</risdate><volume>16</volume><issue>14</issue><spage>1887</spage><epage>1894</epage><pages>1887-1894</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>ABSTRACT
3,4‐Methylenedioxymethamphetamine (MDMA, ecstasy) is a popular drug of abuse. MDMA is pharmacologically classified as an entactogen because of its affinities to classical hallucinogens and stimulants. Oral ingestion of a single dose of the drug is associated with euphoria, elevated self‐confidence, and heightened sensory awareness in humans. Evidence for neurotoxicity in the human serotonin (5‐HT) system has been provided. In rats, a single injection of MDMA induces hyperthermia and formation of reactive oxygen species. These effects may cause MDMA‐associated, long‐term 5‐HT depletion, with the cortex being quite sensitive to the biochemical effects of MDMA. It has been suggested that these MDMA effects may be associated with molecular changes in this brain region. To test these ideas, we have made use of the cDNA array analysis, which can provide a more global view of the molecular changes secondary to MDMA injections. Our results show that the genes regulated by MDMA encode proteins that belong to signaling pathways, transcription regulators, or xenobiotic metabolism. Our observations indicate that cortical cells respond to the acute administration of MDMA by modulating transcription of several genes that might lead to long‐term changes in the brain.—Thiriet, N., Ladenheim, B., McCoy, M. T., Cadet, J. L. Analysis of Ecstasy (MDMA) ‐induced transcriptional responses in the rat cortex. FASEB J. 16, 1887–1894 (2002)</abstract><cop>United States</cop><pub>Federation of American Society of Experimental Biology</pub><pmid>12468453</pmid><doi>10.1096/fj.02-0502com</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals cDNA arrays Cerebral Cortex - metabolism cortex Cytoskeletal Proteins - biosynthesis Cytoskeletal Proteins - genetics Extracellular Matrix Proteins - biosynthesis Extracellular Matrix Proteins - genetics gene expression Gene Expression Profiling Gene Expression Regulation - drug effects Life Sciences MDMA N-Methyl-3,4-methylenedioxyamphetamine - pharmacology Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Rats Rats, Sprague-Dawley Receptors, Serotonin - biosynthesis Receptors, Serotonin - genetics RNA, Messenger - biosynthesis Signal Transduction - drug effects SYBR green PCR Transcription Factors - biosynthesis Transcription Factors - genetics Transcription, Genetic - drug effects |
| title | Analysis of Ecstasy (MDMA)‐induced transcriptional responses in the rat cortex |
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