Expression of protein complexes using multiple Escherichia coli protein co-expression systems: A benchmarking study

Escherichia coli (E. coli) remains the most commonly used host for recombinant protein expression. It is well known that a variety of experimental factors influence the protein production level as well as the solubility profile of over-expressed proteins. This becomes increasingly important for opti...

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Veröffentlicht in:Journal of structural biology 2011-08, Vol.175 (2), p.159-170
Hauptverfasser: Busso, Didier, Peleg, Yoav, Heidebrecht, Tatjana, Romier, Christophe, Jacobovitch, Yossi, Dantes, Ada, Salim, Loubna, Troesch, Edouard, Schuetz, Anja, Heinemann, Udo, Folkers, Gert E., Geerlof, Arie, Wilmanns, Matthias, Polewacz, Andrea, Quedenau, Claudia, Büssow, Konrad, Adamson, Rachel, Blagova, Elena, Walton, Julia, Cartwright, Jared L., Bird, Louise E., Owens, Raymond J., Berrow, Nick S., Wilson, Keith S., Sussman, Joel L., Perrakis, Anastassis, Celie, Patrick H.N.
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Sprache:eng
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