Development of a cockroach (Blattella germanica) qPCR for the objective measurement of exposure at home
Cockroach allergens have a greater impact on asthma morbidity than those from dust mites, cats, and dogs. The American cockroach (Periplaneta americana) and the German cockroach (Blattella germanica) are most frequently responsible for sensitization. The worldwide prevalence of allergic sensitizatio...
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| Veröffentlicht in: | The Science of the total environment 2023-03, Vol.864, p.160965-160965, Article 160965 |
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| creator | Rocchi, Steffi Valot, Benoit Laboissière, Audrey Guitton, Audrey Scherer, Emeline Millon, Laurence Reboux, Gabriel |
| description | Cockroach allergens have a greater impact on asthma morbidity than those from dust mites, cats, and dogs. The American cockroach (Periplaneta americana) and the German cockroach (Blattella germanica) are most frequently responsible for sensitization. The worldwide prevalence of allergic sensitization has been estimated at 2 to 26 % and is influenced by unfavorable socioeconomic conditions. Exposure is generally measured by determining antigen levels in dust or through insect trapping. We developed a real-time quantitative PCR (qPCR) method to provide an objective measurement of B. germanica levels in dwellings.
The specificity of the qPCR primers and TaqMan® hydrolysis probe was validated in silico with 18S rRNA sequences. No amplification was observed for other species of cockroaches, with the exception of Blattella nipponica, which is not common indoors. From 2018 to 2021, exposure to B. germanica was detected and quantified in 27 of 389 dwellings in Bourgogne-Franche-Comté (mean = 333.8; median = 9.1 and maximum = 5304 copy number equivalents) and in 236 of 3193 ELFE cohort dwellings in mainland France in 2011 (mean = 15.6; median < 1 and maximum = 1275 copy number equivalents). The distribution of dwellings testing positive for cockroaches (7 %) differed among the 12 regions of France: |
| doi_str_mv | 10.1016/j.scitotenv.2022.160965 |
| format | Article |
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The specificity of the qPCR primers and TaqMan® hydrolysis probe was validated in silico with 18S rRNA sequences. No amplification was observed for other species of cockroaches, with the exception of Blattella nipponica, which is not common indoors. From 2018 to 2021, exposure to B. germanica was detected and quantified in 27 of 389 dwellings in Bourgogne-Franche-Comté (mean = 333.8; median = 9.1 and maximum = 5304 copy number equivalents) and in 236 of 3193 ELFE cohort dwellings in mainland France in 2011 (mean = 15.6; median < 1 and maximum = 1275 copy number equivalents). The distribution of dwellings testing positive for cockroaches (7 %) differed among the 12 regions of France: <1 % in two regions, between 1 and 5 % in eight regions, 16.5 % in two regions and 35 % around Paris.
Exposure measurements by the EDC sampling and qPCR methods are effective ways to assess the exposure to cockroaches in dwellings. A knowledge of the level of exposure to cockroaches is particularly important for asthmatic patients, particularly those not allergic to other common antigens.
[Display omitted]
•qPCR provides an objective measurement of Blattella germanica levels in dwellings.•Blattella germanica DNA is not only detected in low incomes dwellings and/or where insects are observed.•Blattella germanica detection differs among French regions.•Electrostatic dust collector and qPCR could be a practical and specific tool to assess exposure to cockroaches.•qPCR results would allow better targeting of the prick tests carried out on allergic patients.</description><identifier>ISSN: 0048-9697</identifier><identifier>EISSN: 1879-1026</identifier><identifier>DOI: 10.1016/j.scitotenv.2022.160965</identifier><identifier>PMID: 36526200</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Allergens ; Allergens - analysis ; Animals ; Asthma ; Asthma - epidemiology ; Blattellidae ; Cockroach qPCR ; Dogs ; Dust ; Dwellings ; Electrostatic dust collector ; Hypersensitivity ; Hypersensitivity - epidemiology ; Indoor environment ; Life Sciences ; Polymerase Chain Reaction</subject><ispartof>The Science of the total environment, 2023-03, Vol.864, p.160965-160965, Article 160965</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c400t-c5f5698680254752cfafe76e9afd56823591ea6a79e74e16a0b2532ca883ea883</cites><orcidid>0000-0001-9262-1127 ; 0000-0001-5939-3409</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0048969722080688$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36526200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://univ-fcomte.hal.science/hal-04212326$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rocchi, Steffi</creatorcontrib><creatorcontrib>Valot, Benoit</creatorcontrib><creatorcontrib>Laboissière, Audrey</creatorcontrib><creatorcontrib>Guitton, Audrey</creatorcontrib><creatorcontrib>Scherer, Emeline</creatorcontrib><creatorcontrib>Millon, Laurence</creatorcontrib><creatorcontrib>Reboux, Gabriel</creatorcontrib><title>Development of a cockroach (Blattella germanica) qPCR for the objective measurement of exposure at home</title><title>The Science of the total environment</title><addtitle>Sci Total Environ</addtitle><description>Cockroach allergens have a greater impact on asthma morbidity than those from dust mites, cats, and dogs. The American cockroach (Periplaneta americana) and the German cockroach (Blattella germanica) are most frequently responsible for sensitization. The worldwide prevalence of allergic sensitization has been estimated at 2 to 26 % and is influenced by unfavorable socioeconomic conditions. Exposure is generally measured by determining antigen levels in dust or through insect trapping. We developed a real-time quantitative PCR (qPCR) method to provide an objective measurement of B. germanica levels in dwellings.
The specificity of the qPCR primers and TaqMan® hydrolysis probe was validated in silico with 18S rRNA sequences. No amplification was observed for other species of cockroaches, with the exception of Blattella nipponica, which is not common indoors. From 2018 to 2021, exposure to B. germanica was detected and quantified in 27 of 389 dwellings in Bourgogne-Franche-Comté (mean = 333.8; median = 9.1 and maximum = 5304 copy number equivalents) and in 236 of 3193 ELFE cohort dwellings in mainland France in 2011 (mean = 15.6; median < 1 and maximum = 1275 copy number equivalents). The distribution of dwellings testing positive for cockroaches (7 %) differed among the 12 regions of France: <1 % in two regions, between 1 and 5 % in eight regions, 16.5 % in two regions and 35 % around Paris.
Exposure measurements by the EDC sampling and qPCR methods are effective ways to assess the exposure to cockroaches in dwellings. A knowledge of the level of exposure to cockroaches is particularly important for asthmatic patients, particularly those not allergic to other common antigens.
[Display omitted]
•qPCR provides an objective measurement of Blattella germanica levels in dwellings.•Blattella germanica DNA is not only detected in low incomes dwellings and/or where insects are observed.•Blattella germanica detection differs among French regions.•Electrostatic dust collector and qPCR could be a practical and specific tool to assess exposure to cockroaches.•qPCR results would allow better targeting of the prick tests carried out on allergic patients.</description><subject>Allergens</subject><subject>Allergens - analysis</subject><subject>Animals</subject><subject>Asthma</subject><subject>Asthma - epidemiology</subject><subject>Blattellidae</subject><subject>Cockroach qPCR</subject><subject>Dogs</subject><subject>Dust</subject><subject>Dwellings</subject><subject>Electrostatic dust collector</subject><subject>Hypersensitivity</subject><subject>Hypersensitivity - epidemiology</subject><subject>Indoor environment</subject><subject>Life Sciences</subject><subject>Polymerase Chain Reaction</subject><issn>0048-9697</issn><issn>1879-1026</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFP2zAUxy3ENDq2rwA-wiGd7cR2cizdGJMqgRA7W6_uC3VJ4mK70fbtSRTodT7YsvV7_ye_HyGXnM054-r7bh6tSz5h188FE2LOFauUPCEzXuoq40yoUzJjrCizSlX6jHyJcceGpUv-mZzlSgolGJuR5x_YY-P3LXaJ-poCtd6-BA92S69uGkgJmwboM4YWOmfhmr4-LB9p7QNNW6R-vUObXI-0RYiHgB85-HfvxzuFRLe-xa_kUw1NxG_v5zn5c_vzaXmXre5__V4uVpktGEuZlbVUValKJmShpbA11KgVVlBvpCpFLiuOoEBXqAvkCthayFxYKMscx-2cXE-5W2jMPrgWwj_jwZm7xcqMb6wQXORC9XxgryZ2H_zrAWMyrYt2_G-H_hCN0FJKrbjQA6on1AYfY8D6mM2ZGY2YnTkaMaMRMxkZKi_emxzWLW6OdR8KBmAxATiMpXcYxiDsLG5cGGZrNt79t8kb2QWgTA</recordid><startdate>20230315</startdate><enddate>20230315</enddate><creator>Rocchi, Steffi</creator><creator>Valot, Benoit</creator><creator>Laboissière, Audrey</creator><creator>Guitton, Audrey</creator><creator>Scherer, Emeline</creator><creator>Millon, Laurence</creator><creator>Reboux, Gabriel</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-9262-1127</orcidid><orcidid>https://orcid.org/0000-0001-5939-3409</orcidid></search><sort><creationdate>20230315</creationdate><title>Development of a cockroach (Blattella germanica) qPCR for the objective measurement of exposure at home</title><author>Rocchi, Steffi ; Valot, Benoit ; Laboissière, Audrey ; Guitton, Audrey ; Scherer, Emeline ; Millon, Laurence ; Reboux, Gabriel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-c5f5698680254752cfafe76e9afd56823591ea6a79e74e16a0b2532ca883ea883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Allergens</topic><topic>Allergens - analysis</topic><topic>Animals</topic><topic>Asthma</topic><topic>Asthma - epidemiology</topic><topic>Blattellidae</topic><topic>Cockroach qPCR</topic><topic>Dogs</topic><topic>Dust</topic><topic>Dwellings</topic><topic>Electrostatic dust collector</topic><topic>Hypersensitivity</topic><topic>Hypersensitivity - epidemiology</topic><topic>Indoor environment</topic><topic>Life Sciences</topic><topic>Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rocchi, Steffi</creatorcontrib><creatorcontrib>Valot, Benoit</creatorcontrib><creatorcontrib>Laboissière, Audrey</creatorcontrib><creatorcontrib>Guitton, Audrey</creatorcontrib><creatorcontrib>Scherer, Emeline</creatorcontrib><creatorcontrib>Millon, Laurence</creatorcontrib><creatorcontrib>Reboux, Gabriel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The Science of the total environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rocchi, Steffi</au><au>Valot, Benoit</au><au>Laboissière, Audrey</au><au>Guitton, Audrey</au><au>Scherer, Emeline</au><au>Millon, Laurence</au><au>Reboux, Gabriel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a cockroach (Blattella germanica) qPCR for the objective measurement of exposure at home</atitle><jtitle>The Science of the total environment</jtitle><addtitle>Sci Total Environ</addtitle><date>2023-03-15</date><risdate>2023</risdate><volume>864</volume><spage>160965</spage><epage>160965</epage><pages>160965-160965</pages><artnum>160965</artnum><issn>0048-9697</issn><eissn>1879-1026</eissn><abstract>Cockroach allergens have a greater impact on asthma morbidity than those from dust mites, cats, and dogs. The American cockroach (Periplaneta americana) and the German cockroach (Blattella germanica) are most frequently responsible for sensitization. The worldwide prevalence of allergic sensitization has been estimated at 2 to 26 % and is influenced by unfavorable socioeconomic conditions. Exposure is generally measured by determining antigen levels in dust or through insect trapping. We developed a real-time quantitative PCR (qPCR) method to provide an objective measurement of B. germanica levels in dwellings.
The specificity of the qPCR primers and TaqMan® hydrolysis probe was validated in silico with 18S rRNA sequences. No amplification was observed for other species of cockroaches, with the exception of Blattella nipponica, which is not common indoors. From 2018 to 2021, exposure to B. germanica was detected and quantified in 27 of 389 dwellings in Bourgogne-Franche-Comté (mean = 333.8; median = 9.1 and maximum = 5304 copy number equivalents) and in 236 of 3193 ELFE cohort dwellings in mainland France in 2011 (mean = 15.6; median < 1 and maximum = 1275 copy number equivalents). The distribution of dwellings testing positive for cockroaches (7 %) differed among the 12 regions of France: <1 % in two regions, between 1 and 5 % in eight regions, 16.5 % in two regions and 35 % around Paris.
Exposure measurements by the EDC sampling and qPCR methods are effective ways to assess the exposure to cockroaches in dwellings. A knowledge of the level of exposure to cockroaches is particularly important for asthmatic patients, particularly those not allergic to other common antigens.
[Display omitted]
•qPCR provides an objective measurement of Blattella germanica levels in dwellings.•Blattella germanica DNA is not only detected in low incomes dwellings and/or where insects are observed.•Blattella germanica detection differs among French regions.•Electrostatic dust collector and qPCR could be a practical and specific tool to assess exposure to cockroaches.•qPCR results would allow better targeting of the prick tests carried out on allergic patients.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36526200</pmid><doi>10.1016/j.scitotenv.2022.160965</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-9262-1127</orcidid><orcidid>https://orcid.org/0000-0001-5939-3409</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Allergens Allergens - analysis Animals Asthma Asthma - epidemiology Blattellidae Cockroach qPCR Dogs Dust Dwellings Electrostatic dust collector Hypersensitivity Hypersensitivity - epidemiology Indoor environment Life Sciences Polymerase Chain Reaction |
| title | Development of a cockroach (Blattella germanica) qPCR for the objective measurement of exposure at home |
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