Separation and Quantification by Ion-Association Capillary Zone Electrophoresis of Unsaturated Disaccharide Units of Chondroitin Sulfates and Oligosaccharides Derived from Hyaluronan
An analytical method has been developed for assay of unsaturated disaccharides of chondroitin sulfates and of oligosaccharides (tetra- and hexasaccharides) of hyaluronan, using ion-association capillary zone electrophoresis. Samples were applied at the anode (the usual polarity), using a borate buff...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1998-11, Vol.70 (22), p.4780-4786 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Payan, E Presle, N Lapicque, F Jouzeau, J. Y Bordji, K Oerther, S Miralles, G Mainard, D Netter, P |
| description | An analytical method has been developed for assay of unsaturated disaccharides of chondroitin sulfates and of oligosaccharides (tetra- and hexasaccharides) of hyaluronan, using ion-association capillary zone electrophoresis. Samples were applied at the anode (the usual polarity), using a borate buffer modified by an ion-pairing reagent, tetrabutylammonium (TBA) phosphate, and the effect of the concentration of the ion-pairing reagent on various electrophoretic parameters (electroosmotic flow, electrophoretic mobility of products, capacity factors) was observed. Increasing concentrations of the reagent led to a decrease of ζ potential, probably due to specific adsorption of the quaternary ammonium ion onto the capillary wall. The authors propose a mechanism of separation, in which anionic borate complexes are formed and interact with TBA ion inside the capillary tube. The capillary electrophoretic system described is potentially a powerful method for specific measurement of the concentrations in joint tissues of chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronan, whose relative abundances may vary in various diseases or after local treatments with, for example, antiinflammatory drugs, chondroprotective agents, or orthopedic implants. |
| doi_str_mv | 10.1021/ac9800558 |
| format | Article |
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Y ; Bordji, K ; Oerther, S ; Miralles, G ; Mainard, D ; Netter, P</creator><creatorcontrib>Payan, E ; Presle, N ; Lapicque, F ; Jouzeau, J. Y ; Bordji, K ; Oerther, S ; Miralles, G ; Mainard, D ; Netter, P</creatorcontrib><description>An analytical method has been developed for assay of unsaturated disaccharides of chondroitin sulfates and of oligosaccharides (tetra- and hexasaccharides) of hyaluronan, using ion-association capillary zone electrophoresis. Samples were applied at the anode (the usual polarity), using a borate buffer modified by an ion-pairing reagent, tetrabutylammonium (TBA) phosphate, and the effect of the concentration of the ion-pairing reagent on various electrophoretic parameters (electroosmotic flow, electrophoretic mobility of products, capacity factors) was observed. Increasing concentrations of the reagent led to a decrease of ζ potential, probably due to specific adsorption of the quaternary ammonium ion onto the capillary wall. The authors propose a mechanism of separation, in which anionic borate complexes are formed and interact with TBA ion inside the capillary tube. The capillary electrophoretic system described is potentially a powerful method for specific measurement of the concentrations in joint tissues of chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronan, whose relative abundances may vary in various diseases or after local treatments with, for example, antiinflammatory drugs, chondroprotective agents, or orthopedic implants.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac9800558</identifier><identifier>PMID: 9844574</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Carbohydrates ; Chemistry ; Chondroitin Sulfates - analysis ; Disaccharides - analysis ; Electrophoresis, Capillary ; Fundamental and applied biological sciences. Psychology ; Heterosides ; Hyaluronic Acid - analysis ; Indicators and Reagents ; Life Sciences ; Oligosaccharides - analysis ; Other biological molecules</subject><ispartof>Analytical chemistry (Washington), 1998-11, Vol.70 (22), p.4780-4786</ispartof><rights>Copyright © 1998 American Chemical Society</rights><rights>1999 INIST-CNRS</rights><rights>Copyright American Chemical Society Nov 15, 1998</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a504t-e8476925fceeda57ae8f1f62b5a332377f1f4a7c93694400c93ebab5b0c8e7613</citedby><cites>FETCH-LOGICAL-a504t-e8476925fceeda57ae8f1f62b5a332377f1f4a7c93694400c93ebab5b0c8e7613</cites><orcidid>0000-0003-1944-1538 ; 0000-0003-4088-4181</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac9800558$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac9800558$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1619308$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9844574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04191008$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Payan, E</creatorcontrib><creatorcontrib>Presle, N</creatorcontrib><creatorcontrib>Lapicque, F</creatorcontrib><creatorcontrib>Jouzeau, J. Y</creatorcontrib><creatorcontrib>Bordji, K</creatorcontrib><creatorcontrib>Oerther, S</creatorcontrib><creatorcontrib>Miralles, G</creatorcontrib><creatorcontrib>Mainard, D</creatorcontrib><creatorcontrib>Netter, P</creatorcontrib><title>Separation and Quantification by Ion-Association Capillary Zone Electrophoresis of Unsaturated Disaccharide Units of Chondroitin Sulfates and Oligosaccharides Derived from Hyaluronan</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>An analytical method has been developed for assay of unsaturated disaccharides of chondroitin sulfates and of oligosaccharides (tetra- and hexasaccharides) of hyaluronan, using ion-association capillary zone electrophoresis. Samples were applied at the anode (the usual polarity), using a borate buffer modified by an ion-pairing reagent, tetrabutylammonium (TBA) phosphate, and the effect of the concentration of the ion-pairing reagent on various electrophoretic parameters (electroosmotic flow, electrophoretic mobility of products, capacity factors) was observed. Increasing concentrations of the reagent led to a decrease of ζ potential, probably due to specific adsorption of the quaternary ammonium ion onto the capillary wall. The authors propose a mechanism of separation, in which anionic borate complexes are formed and interact with TBA ion inside the capillary tube. The capillary electrophoretic system described is potentially a powerful method for specific measurement of the concentrations in joint tissues of chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronan, whose relative abundances may vary in various diseases or after local treatments with, for example, antiinflammatory drugs, chondroprotective agents, or orthopedic implants.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Chemistry</subject><subject>Chondroitin Sulfates - analysis</subject><subject>Disaccharides - analysis</subject><subject>Electrophoresis, Capillary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heterosides</subject><subject>Hyaluronic Acid - analysis</subject><subject>Indicators and Reagents</subject><subject>Life Sciences</subject><subject>Oligosaccharides - analysis</subject><subject>Other biological molecules</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkV1v0zAUhiMEGmVwwQ9AshAgcRE4zpedy6r76KRKK-omJm6sE8ehHqkd7GRa_xi_D3epWgmubJ_38fv6-ETRWwpfKCT0K8qSA-Q5fxZNaJ5AXHCePI8mAJDGCQN4Gb3y_h6AUqDFSXRS8izLWTaJ_qxUhw57bQ1BU5NvA5peN1qOpWpLrqyJp95bqcfSDDvdtui25Ic1ipy3SvbOdmvrlNee2IbcGo_9EExVTc60RynX6HStgqD7J2K2tqZ2VvfakNXQNgH1T_HXrf5pjzc8OVNOPwSfxtkNmW-xHZw1aF5HLxpsvXqzX0-j24vzm9k8XlxfXs2mixhzyPpY8YwVZZI3Uqkac4aKN7QpkirHNE1SxsIpQybLtCizDCBsVIVVXoHkihU0PY0-j75rbEXn9Cb0LSxqMZ8uxK4GGS0pAH_YsZ9GtnP296B8LzbaSxX-yig7eMGAQsFYEsD3_4D3dnAm9CESyniRpsCPydJZ751qDvEUxG7o4jD0wL7bGw7VRtUHcj_loH_Y6-glto1DI7U_Gha0HCPjEdO-V48HGd0vUbCU5eJmuRJL9p0t7_iduAz8x5FH6Y8t_P-8v0AI0Wg</recordid><startdate>19981115</startdate><enddate>19981115</enddate><creator>Payan, E</creator><creator>Presle, N</creator><creator>Lapicque, F</creator><creator>Jouzeau, J. 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Y</au><au>Bordji, K</au><au>Oerther, S</au><au>Miralles, G</au><au>Mainard, D</au><au>Netter, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and Quantification by Ion-Association Capillary Zone Electrophoresis of Unsaturated Disaccharide Units of Chondroitin Sulfates and Oligosaccharides Derived from Hyaluronan</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1998-11-15</date><risdate>1998</risdate><volume>70</volume><issue>22</issue><spage>4780</spage><epage>4786</epage><pages>4780-4786</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>An analytical method has been developed for assay of unsaturated disaccharides of chondroitin sulfates and of oligosaccharides (tetra- and hexasaccharides) of hyaluronan, using ion-association capillary zone electrophoresis. Samples were applied at the anode (the usual polarity), using a borate buffer modified by an ion-pairing reagent, tetrabutylammonium (TBA) phosphate, and the effect of the concentration of the ion-pairing reagent on various electrophoretic parameters (electroosmotic flow, electrophoretic mobility of products, capacity factors) was observed. Increasing concentrations of the reagent led to a decrease of ζ potential, probably due to specific adsorption of the quaternary ammonium ion onto the capillary wall. The authors propose a mechanism of separation, in which anionic borate complexes are formed and interact with TBA ion inside the capillary tube. The capillary electrophoretic system described is potentially a powerful method for specific measurement of the concentrations in joint tissues of chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronan, whose relative abundances may vary in various diseases or after local treatments with, for example, antiinflammatory drugs, chondroprotective agents, or orthopedic implants.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>9844574</pmid><doi>10.1021/ac9800558</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1944-1538</orcidid><orcidid>https://orcid.org/0000-0003-4088-4181</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical chemistry (Washington), 1998-11, Vol.70 (22), p.4780-4786 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrates Chemistry Chondroitin Sulfates - analysis Disaccharides - analysis Electrophoresis, Capillary Fundamental and applied biological sciences. Psychology Heterosides Hyaluronic Acid - analysis Indicators and Reagents Life Sciences Oligosaccharides - analysis Other biological molecules |
| title | Separation and Quantification by Ion-Association Capillary Zone Electrophoresis of Unsaturated Disaccharide Units of Chondroitin Sulfates and Oligosaccharides Derived from Hyaluronan |
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