Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol
Genetically-modified Enterococcus faecalis has a potential of survival and can be used in ethanolic fermentations. Fermentation profiles of E. faecalis JH2-2 were assessed using glucose and lactose as carbon sources. Deletion of lactate dehydrogenase ( ldh ) genes increased the ethanol production fr...
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| Veröffentlicht in: | Biotechnology letters 2012-09, Vol.34 (9), p.1651-1657 |
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| creator | Rana, N. F. Gente, S. Rincé, A. Auffray, Y. Laplace, J. M. |
| description | Genetically-modified
Enterococcus faecalis
has a potential of survival and can be used in ethanolic fermentations. Fermentation profiles of
E. faecalis
JH2-2 were assessed using glucose and lactose as carbon sources. Deletion of lactate dehydrogenase (
ldh
) genes increased the ethanol production from 0.25 to 0.82 g/l, which was further increased to 0.96 g/l by the insertion of a pyruvate decarboxylase (
pdc
) gene (from
Sarcina ventriculi
or
Clostridium
acetobutylicum
) in place
ldh1
. When grown on lactose, the
pdcSv
and
pdcCa
showed 13.6 and 17.6 U mg
−1
of
pdc
specific activity, respectively. Highest activity (47 U mg
−1
) and ethanol concentration (2.3 g/l) were obtained with
pdcCa
using an expression plasmid. Formate and acetate were also produced in high quantities. Transcriptional analysis showed that aldehyde alcohol dehydrogenase gene was upregulated up to 16-fold. Further optimizations are required for higher ethanol production. |
| doi_str_mv | 10.1007/s10529-012-0964-x |
| format | Article |
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Enterococcus faecalis
has a potential of survival and can be used in ethanolic fermentations. Fermentation profiles of
E. faecalis
JH2-2 were assessed using glucose and lactose as carbon sources. Deletion of lactate dehydrogenase (
ldh
) genes increased the ethanol production from 0.25 to 0.82 g/l, which was further increased to 0.96 g/l by the insertion of a pyruvate decarboxylase (
pdc
) gene (from
Sarcina ventriculi
or
Clostridium
acetobutylicum
) in place
ldh1
. When grown on lactose, the
pdcSv
and
pdcCa
showed 13.6 and 17.6 U mg
−1
of
pdc
specific activity, respectively. Highest activity (47 U mg
−1
) and ethanol concentration (2.3 g/l) were obtained with
pdcCa
using an expression plasmid. Formate and acetate were also produced in high quantities. Transcriptional analysis showed that aldehyde alcohol dehydrogenase gene was upregulated up to 16-fold. Further optimizations are required for higher ethanol production.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-012-0964-x</identifier><identifier>PMID: 22628022</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Acetic Acid - metabolism ; Applied Microbiology ; Bacteria ; Biochemistry ; Biodiesel fuels ; Biofuels ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Carbon sources ; Clostridium acetobutylicum ; Clostridium acetobutylicum - enzymology ; Clostridium acetobutylicum - genetics ; Dehydrogenase ; Enterococcus faecalis ; Enterococcus faecalis - genetics ; Enterococcus faecalis - metabolism ; Ethanol ; Ethanol - metabolism ; Fermentation ; Formates - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Gene Knockout Techniques ; Genetic engineering ; Glucose - metabolism ; Hydro-Lyases - genetics ; Hydro-Lyases - metabolism ; Lactose - metabolism ; Life Sciences ; Metabolic Engineering ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Microbiology ; Organisms, Genetically Modified ; Original Research Paper ; Pyruvate Dehydrogenase Complex - genetics ; Pyruvate Dehydrogenase Complex - metabolism ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Sarcina</subject><ispartof>Biotechnology letters, 2012-09, Vol.34 (9), p.1651-1657</ispartof><rights>Springer Science+Business Media B.V. 2012</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-43b90a0a29de1dd89cc0f7293cf0152f2182eaf965bdaf45fc39912b7acdeca63</citedby><cites>FETCH-LOGICAL-c535t-43b90a0a29de1dd89cc0f7293cf0152f2182eaf965bdaf45fc39912b7acdeca63</cites><orcidid>0000-0002-0165-7288</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-012-0964-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-012-0964-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26211909$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22628022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://normandie-univ.hal.science/hal-04158326$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rana, N. F.</creatorcontrib><creatorcontrib>Gente, S.</creatorcontrib><creatorcontrib>Rincé, A.</creatorcontrib><creatorcontrib>Auffray, Y.</creatorcontrib><creatorcontrib>Laplace, J. M.</creatorcontrib><title>Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Genetically-modified
Enterococcus faecalis
has a potential of survival and can be used in ethanolic fermentations. Fermentation profiles of
E. faecalis
JH2-2 were assessed using glucose and lactose as carbon sources. Deletion of lactate dehydrogenase (
ldh
) genes increased the ethanol production from 0.25 to 0.82 g/l, which was further increased to 0.96 g/l by the insertion of a pyruvate decarboxylase (
pdc
) gene (from
Sarcina ventriculi
or
Clostridium
acetobutylicum
) in place
ldh1
. When grown on lactose, the
pdcSv
and
pdcCa
showed 13.6 and 17.6 U mg
−1
of
pdc
specific activity, respectively. Highest activity (47 U mg
−1
) and ethanol concentration (2.3 g/l) were obtained with
pdcCa
using an expression plasmid. Formate and acetate were also produced in high quantities. Transcriptional analysis showed that aldehyde alcohol dehydrogenase gene was upregulated up to 16-fold. Further optimizations are required for higher ethanol production.</description><subject>Acetic Acid - metabolism</subject><subject>Applied Microbiology</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biodiesel fuels</subject><subject>Biofuels</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Carbon sources</subject><subject>Clostridium acetobutylicum</subject><subject>Clostridium acetobutylicum - enzymology</subject><subject>Clostridium acetobutylicum - genetics</subject><subject>Dehydrogenase</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecalis - genetics</subject><subject>Enterococcus faecalis - metabolism</subject><subject>Ethanol</subject><subject>Ethanol - metabolism</subject><subject>Fermentation</subject><subject>Formates - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Knockout Techniques</subject><subject>Genetic engineering</subject><subject>Glucose - metabolism</subject><subject>Hydro-Lyases - genetics</subject><subject>Hydro-Lyases - metabolism</subject><subject>Lactose - metabolism</subject><subject>Life Sciences</subject><subject>Metabolic Engineering</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Microbiology</subject><subject>Organisms, Genetically Modified</subject><subject>Original Research Paper</subject><subject>Pyruvate Dehydrogenase Complex - genetics</subject><subject>Pyruvate Dehydrogenase Complex - metabolism</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sarcina</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU2LFDEQhoMo7jj6A7xIQAQ9tKaS7mRyXJZ1VxjwoueQzsdMlp5kTLqX9eg_N22P6wcInhLqfarqLV6EngN5C4SIdwVIR2VDgDZE8ra5e4BW0AnWcCH4Q7Qi0ELTtZKeoSel3BBCpCDiMTqjlNMNoXSFvl256MZg8CHZ4IPRY0ixYB0tDnHMyU5mruDk8d6NLqch7dJU8NEavKutpWL4Ms6KScZUxWtn9BDqJ2UcxoKn4mbo-MewPiQ37nVMw1P0yOuhuGend40-v7_8dHHdbD9efbg43zamY93YtKyXRBNNpXVg7UYaQ7ygkhlPoKOewoY67SXveqt923nDpATaC21sNcTZGr1Z5u71oI45HHT-qpIO6vp8q-YaaaHbMMpvobKvF7aa_jK5MqpDKMYNg46uXq-ASNYKYIz-B8oE55S1rKIv_0Jv0pRjPfoHxYABmXfDQpmcSsnO35sFoubY1RK7qrGrOXZ1V3tenCZP_cHZ-46fOVfg1QnQpabjs44mlF8cpwCyHrVGdOFKleLO5d8t_mv7d7L5xig</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Rana, N. 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F. ; Gente, S. ; Rincé, A. ; Auffray, Y. ; Laplace, J. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-43b90a0a29de1dd89cc0f7293cf0152f2182eaf965bdaf45fc39912b7acdeca63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acetic Acid - metabolism</topic><topic>Applied Microbiology</topic><topic>Bacteria</topic><topic>Biochemistry</topic><topic>Biodiesel fuels</topic><topic>Biofuels</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Carbon sources</topic><topic>Clostridium acetobutylicum</topic><topic>Clostridium acetobutylicum - enzymology</topic><topic>Clostridium acetobutylicum - genetics</topic><topic>Dehydrogenase</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecalis - genetics</topic><topic>Enterococcus faecalis - metabolism</topic><topic>Ethanol</topic><topic>Ethanol - metabolism</topic><topic>Fermentation</topic><topic>Formates - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Knockout Techniques</topic><topic>Genetic engineering</topic><topic>Glucose - metabolism</topic><topic>Hydro-Lyases - genetics</topic><topic>Hydro-Lyases - metabolism</topic><topic>Lactose - metabolism</topic><topic>Life Sciences</topic><topic>Metabolic Engineering</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Microbiology</topic><topic>Organisms, Genetically Modified</topic><topic>Original Research Paper</topic><topic>Pyruvate Dehydrogenase Complex - genetics</topic><topic>Pyruvate Dehydrogenase Complex - metabolism</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Sarcina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rana, N. 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F.</au><au>Gente, S.</au><au>Rincé, A.</au><au>Auffray, Y.</au><au>Laplace, J. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>34</volume><issue>9</issue><spage>1651</spage><epage>1657</epage><pages>1651-1657</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Genetically-modified
Enterococcus faecalis
has a potential of survival and can be used in ethanolic fermentations. Fermentation profiles of
E. faecalis
JH2-2 were assessed using glucose and lactose as carbon sources. Deletion of lactate dehydrogenase (
ldh
) genes increased the ethanol production from 0.25 to 0.82 g/l, which was further increased to 0.96 g/l by the insertion of a pyruvate decarboxylase (
pdc
) gene (from
Sarcina ventriculi
or
Clostridium
acetobutylicum
) in place
ldh1
. When grown on lactose, the
pdcSv
and
pdcCa
showed 13.6 and 17.6 U mg
−1
of
pdc
specific activity, respectively. Highest activity (47 U mg
−1
) and ethanol concentration (2.3 g/l) were obtained with
pdcCa
using an expression plasmid. Formate and acetate were also produced in high quantities. Transcriptional analysis showed that aldehyde alcohol dehydrogenase gene was upregulated up to 16-fold. Further optimizations are required for higher ethanol production.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>22628022</pmid><doi>10.1007/s10529-012-0964-x</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-0165-7288</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 2012-09, Vol.34 (9), p.1651-1657 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_04158326v1 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Acetic Acid - metabolism Applied Microbiology Bacteria Biochemistry Biodiesel fuels Biofuels Biological and medical sciences Biomedical and Life Sciences Biotechnology Carbon sources Clostridium acetobutylicum Clostridium acetobutylicum - enzymology Clostridium acetobutylicum - genetics Dehydrogenase Enterococcus faecalis Enterococcus faecalis - genetics Enterococcus faecalis - metabolism Ethanol Ethanol - metabolism Fermentation Formates - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Profiling Gene Expression Regulation, Bacterial Gene Knockout Techniques Genetic engineering Glucose - metabolism Hydro-Lyases - genetics Hydro-Lyases - metabolism Lactose - metabolism Life Sciences Metabolic Engineering Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Microbiology Organisms, Genetically Modified Original Research Paper Pyruvate Dehydrogenase Complex - genetics Pyruvate Dehydrogenase Complex - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Sarcina |
| title | Genetic modifications and introduction of heterologous pdc genes in Enterococcus faecalis for its use in production of bioethanol |
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