In vitro follicular growth affects oocyte imprinting establishment in mice
In vitro folliculogenesis of cryopreserved ovarian tissue could be an effective method for insuring fertility for patients who receive gonadotoxic treatment. Although several culture systems have been described for growing female gametes in vitro , the production of competent oocytes for further dev...
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| Veröffentlicht in: | European journal of human genetics : EJHG 2003-07, Vol.11 (7), p.493-496 |
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| container_title | European journal of human genetics : EJHG |
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| creator | Kerjean, Antoine Couvert, Philippe Heams, Thomas Chalas, Céline Poirier, Karine Chelly, Jamel Jouannet, Pierre Paldi, Andras Poirot, Catherine |
| description | In vitro
folliculogenesis of cryopreserved ovarian tissue could be an effective method for insuring fertility for patients who receive gonadotoxic treatment. Although several culture systems have been described for growing female gametes
in vitro
, the production of competent oocytes for further development remains a considerable challenge. The purpose of our study was to determine whether maternal primary imprinting progresses normally during mouse oocyte growth
in vitro
. We analysed the DNA methylation status of differentially methylated regions of the imprinted genes
H19
,
Mest/Peg1
and
Igf2R
using fully grown germinal vesicle-stage oocytes (fg oocytes) produced by
in vitro
folliculogenesis from early preantral follicles. When compared to fg oocytes removal from control females, we observed after
in vitro
development, a loss of methylation at the
Igf2R
locus in six out of seven independent experiments and
Mest/Peg1
locus (one out of seven), and a gain of methylation at the
H19
locus (one out of seven). These results provide insight into the dysregulation of the process of primary imprinting during oocyte growth
in vitro
and highlight the need for effective new biomarkers to identify complete nuclear reprogramming competence after
in vitro
folliculogenesis. |
| doi_str_mv | 10.1038/sj.ejhg.5200990 |
| format | Article |
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folliculogenesis of cryopreserved ovarian tissue could be an effective method for insuring fertility for patients who receive gonadotoxic treatment. Although several culture systems have been described for growing female gametes
in vitro
, the production of competent oocytes for further development remains a considerable challenge. The purpose of our study was to determine whether maternal primary imprinting progresses normally during mouse oocyte growth
in vitro
. We analysed the DNA methylation status of differentially methylated regions of the imprinted genes
H19
,
Mest/Peg1
and
Igf2R
using fully grown germinal vesicle-stage oocytes (fg oocytes) produced by
in vitro
folliculogenesis from early preantral follicles. When compared to fg oocytes removal from control females, we observed after
in vitro
development, a loss of methylation at the
Igf2R
locus in six out of seven independent experiments and
Mest/Peg1
locus (one out of seven), and a gain of methylation at the
H19
locus (one out of seven). These results provide insight into the dysregulation of the process of primary imprinting during oocyte growth
in vitro
and highlight the need for effective new biomarkers to identify complete nuclear reprogramming competence after
in vitro
folliculogenesis.</description><identifier>ISSN: 1018-4813</identifier><identifier>EISSN: 1476-5438</identifier><identifier>DOI: 10.1038/sj.ejhg.5200990</identifier><identifier>PMID: 12825069</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Bioinformatics ; Biological and medical sciences ; Biomarkers ; Biomedical and Life Sciences ; Biomedicine ; Cytogenetics ; DNA Methylation ; Female ; Gene Expression ; Genetics ; Genital system. Mammary gland ; Genomic Imprinting - physiology ; Human Genetics ; Investigative techniques, diagnostic techniques (general aspects) ; Life Sciences ; Medical sciences ; Mice ; Oocytes - physiology ; Ovarian Follicle - growth & development ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Polymerase Chain Reaction ; Proteins - genetics ; RNA, Long Noncoding ; RNA, Untranslated</subject><ispartof>European journal of human genetics : EJHG, 2003-07, Vol.11 (7), p.493-496</ispartof><rights>Springer Nature Switzerland AG 2003</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jul 2003</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-76b1303975eb351347a343cd44719df19cb0068d1627eee5bbf77b5ce80415f33</citedby><cites>FETCH-LOGICAL-c532t-76b1303975eb351347a343cd44719df19cb0068d1627eee5bbf77b5ce80415f33</cites><orcidid>0000-0003-0128-0115 ; 0000-0003-4446-7625</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/sj.ejhg.5200990$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/sj.ejhg.5200990$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14972995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12825069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04144186$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Kerjean, Antoine</creatorcontrib><creatorcontrib>Couvert, Philippe</creatorcontrib><creatorcontrib>Heams, Thomas</creatorcontrib><creatorcontrib>Chalas, Céline</creatorcontrib><creatorcontrib>Poirier, Karine</creatorcontrib><creatorcontrib>Chelly, Jamel</creatorcontrib><creatorcontrib>Jouannet, Pierre</creatorcontrib><creatorcontrib>Paldi, Andras</creatorcontrib><creatorcontrib>Poirot, Catherine</creatorcontrib><title>In vitro follicular growth affects oocyte imprinting establishment in mice</title><title>European journal of human genetics : EJHG</title><addtitle>Eur J Hum Genet</addtitle><addtitle>Eur J Hum Genet</addtitle><description>In vitro
folliculogenesis of cryopreserved ovarian tissue could be an effective method for insuring fertility for patients who receive gonadotoxic treatment. Although several culture systems have been described for growing female gametes
in vitro
, the production of competent oocytes for further development remains a considerable challenge. The purpose of our study was to determine whether maternal primary imprinting progresses normally during mouse oocyte growth
in vitro
. We analysed the DNA methylation status of differentially methylated regions of the imprinted genes
H19
,
Mest/Peg1
and
Igf2R
using fully grown germinal vesicle-stage oocytes (fg oocytes) produced by
in vitro
folliculogenesis from early preantral follicles. When compared to fg oocytes removal from control females, we observed after
in vitro
development, a loss of methylation at the
Igf2R
locus in six out of seven independent experiments and
Mest/Peg1
locus (one out of seven), and a gain of methylation at the
H19
locus (one out of seven). These results provide insight into the dysregulation of the process of primary imprinting during oocyte growth
in vitro
and highlight the need for effective new biomarkers to identify complete nuclear reprogramming competence after
in vitro
folliculogenesis.</description><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cytogenetics</subject><subject>DNA Methylation</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Genetics</subject><subject>Genital system. Mammary gland</subject><subject>Genomic Imprinting - physiology</subject><subject>Human Genetics</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Life Sciences</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Oocytes - physiology</subject><subject>Ovarian Follicle - growth & development</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. 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Mammary gland</topic><topic>Genomic Imprinting - physiology</topic><topic>Human Genetics</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Life Sciences</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Oocytes - physiology</topic><topic>Ovarian Follicle - growth & development</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. 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folliculogenesis of cryopreserved ovarian tissue could be an effective method for insuring fertility for patients who receive gonadotoxic treatment. Although several culture systems have been described for growing female gametes
in vitro
, the production of competent oocytes for further development remains a considerable challenge. The purpose of our study was to determine whether maternal primary imprinting progresses normally during mouse oocyte growth
in vitro
. We analysed the DNA methylation status of differentially methylated regions of the imprinted genes
H19
,
Mest/Peg1
and
Igf2R
using fully grown germinal vesicle-stage oocytes (fg oocytes) produced by
in vitro
folliculogenesis from early preantral follicles. When compared to fg oocytes removal from control females, we observed after
in vitro
development, a loss of methylation at the
Igf2R
locus in six out of seven independent experiments and
Mest/Peg1
locus (one out of seven), and a gain of methylation at the
H19
locus (one out of seven). These results provide insight into the dysregulation of the process of primary imprinting during oocyte growth
in vitro
and highlight the need for effective new biomarkers to identify complete nuclear reprogramming competence after
in vitro
folliculogenesis.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>12825069</pmid><doi>10.1038/sj.ejhg.5200990</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-0128-0115</orcidid><orcidid>https://orcid.org/0000-0003-4446-7625</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1018-4813 |
| ispartof | European journal of human genetics : EJHG, 2003-07, Vol.11 (7), p.493-496 |
| issn | 1018-4813 1476-5438 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_04144186v1 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; SpringerLink Journals - AutoHoldings |
| subjects | Animals Bioinformatics Biological and medical sciences Biomarkers Biomedical and Life Sciences Biomedicine Cytogenetics DNA Methylation Female Gene Expression Genetics Genital system. Mammary gland Genomic Imprinting - physiology Human Genetics Investigative techniques, diagnostic techniques (general aspects) Life Sciences Medical sciences Mice Oocytes - physiology Ovarian Follicle - growth & development Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Proteins - genetics RNA, Long Noncoding RNA, Untranslated |
| title | In vitro follicular growth affects oocyte imprinting establishment in mice |
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