Investigation of the Plutonium(IV) Interactions with Two Variants of the EF-Hand Ca-Binding Site I of Calmodulin
Due to its presence in the nuclear industry and its strong radiotoxicity, plutonium is an actinide of major interest in the event of internal contamination. To improve the understanding of its mechanisms of transport and accumulation in the body, the complexation of Pu(IV) to the most common protei...
Gespeichert in:
| Veröffentlicht in: | Inorganic chemistry 2023-05, Vol.62 (21), p.8334-8346 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 8346 |
|---|---|
| container_issue | 21 |
| container_start_page | 8334 |
| container_title | Inorganic chemistry |
| container_volume | 62 |
| creator | Daronnat, Loïc Holfeltz, Vanessa Boubals, Nathalie Dumas, Thomas Guilbaud, Philippe Martinez, Diego Moreno Moisy, Philippe Sauge-Merle, Sandrine Lemaire, David Solari, Pier Lorenzo Berthon, Laurence Berthomieu, Catherine |
| description | Due to its presence in the nuclear industry and its strong radiotoxicity, plutonium is an actinide of major interest in the event of internal contamination. To improve the understanding of its mechanisms of transport and accumulation in the body, the complexation of Pu(IV) to the most common protein calcium-binding motif found in cells, the EF-hand motif of calmodulin, was investigated. Visible and X-ray absorption spectroscopies (XAS) in solution made it possible to investigate the speciation of plutonium at physiological pH (pH 7.4) and pH 6 in two variants of the calmodulin Ca-binding site I and using Pu(IV) in different media: carbonate, chloride, or nitrate solutions. Three different species of Pu were identified in the samples, with formation of 1:1 Pu(IV):calmodulin peptide complexes, Pu(IV) reduction, and formation of peptide-mediated Pu(IV) hexanuclear cluster. |
| doi_str_mv | 10.1021/acs.inorgchem.3c00845 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_04102795v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2813889037</sourcerecordid><originalsourceid>FETCH-LOGICAL-a333t-2ef91125650cf672034683b8f515312ec43ea68b9de96709dd8fbb365ee1a27d3</originalsourceid><addsrcrecordid>eNqFkVFv2yAUhdG0qc3a_oRNPLYPzi7GYPzYRe1iKdImrav6hrCNEyobMsCt-u-HlTSve-IKfefcq3MQ-kJgSSAn31QblsY6v213elzSFkAU7ANaEJZDxgg8fUQLgDQTzqtz9DmEZwCoaMHP0DktiSgoLxZoX9sXHaLZqmicxa7Hcafxr2GKzpppvK4fb3Bto_aqnYGAX03c4YdXhx-VN8rG8K65u8_WynZ4pbLvxnbGbvFvEzWuZ2ClhtF102DsJfrUqyHoq-N7gf7c3z2s1tnm5496dbvJFKU0ZrnuK0Jyxhm0PS9zSIcL2oieEUZJrtuCasVFU3W64iVUXSf6pqGcaU1UXnb0At0cfHdqkHtvRuXfpFNGrm83cv6DIuVYVuyFJPb6wO69-zulOORoQquHQVntpiBzQagQFdAyoeyAtt6F4HV_8iYg52JkKkaeipHHYpLu63HF1Iy6O6nem0gAOQCz_tlN3qZ0_mP6D5OynI0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2813889037</pqid></control><display><type>article</type><title>Investigation of the Plutonium(IV) Interactions with Two Variants of the EF-Hand Ca-Binding Site I of Calmodulin</title><source>ACS Publications</source><source>MEDLINE</source><creator>Daronnat, Loïc ; Holfeltz, Vanessa ; Boubals, Nathalie ; Dumas, Thomas ; Guilbaud, Philippe ; Martinez, Diego Moreno ; Moisy, Philippe ; Sauge-Merle, Sandrine ; Lemaire, David ; Solari, Pier Lorenzo ; Berthon, Laurence ; Berthomieu, Catherine</creator><creatorcontrib>Daronnat, Loïc ; Holfeltz, Vanessa ; Boubals, Nathalie ; Dumas, Thomas ; Guilbaud, Philippe ; Martinez, Diego Moreno ; Moisy, Philippe ; Sauge-Merle, Sandrine ; Lemaire, David ; Solari, Pier Lorenzo ; Berthon, Laurence ; Berthomieu, Catherine</creatorcontrib><description>Due to its presence in the nuclear industry and its strong radiotoxicity, plutonium is an actinide of major interest in the event of internal contamination. To improve the understanding of its mechanisms of transport and accumulation in the body, the complexation of Pu(IV) to the most common protein calcium-binding motif found in cells, the EF-hand motif of calmodulin, was investigated. Visible and X-ray absorption spectroscopies (XAS) in solution made it possible to investigate the speciation of plutonium at physiological pH (pH 7.4) and pH 6 in two variants of the calmodulin Ca-binding site I and using Pu(IV) in different media: carbonate, chloride, or nitrate solutions. Three different species of Pu were identified in the samples, with formation of 1:1 Pu(IV):calmodulin peptide complexes, Pu(IV) reduction, and formation of peptide-mediated Pu(IV) hexanuclear cluster.</description><identifier>ISSN: 0020-1669</identifier><identifier>EISSN: 1520-510X</identifier><identifier>DOI: 10.1021/acs.inorgchem.3c00845</identifier><identifier>PMID: 37184364</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Binding Sites ; Calcium ; Calmodulin ; Life Sciences ; Oxidation-Reduction ; Plutonium - chemistry</subject><ispartof>Inorganic chemistry, 2023-05, Vol.62 (21), p.8334-8346</ispartof><rights>2023 American Chemical Society</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-a333t-2ef91125650cf672034683b8f515312ec43ea68b9de96709dd8fbb365ee1a27d3</cites><orcidid>0000-0003-3474-8474 ; 0000-0001-6425-6484 ; 0000-0002-9331-0846 ; 0000-0001-9777-8326 ; 0000-0001-8784-5396 ; 0000-0003-2721-3885</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.inorgchem.3c00845$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.inorgchem.3c00845$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37184364$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04102795$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Daronnat, Loïc</creatorcontrib><creatorcontrib>Holfeltz, Vanessa</creatorcontrib><creatorcontrib>Boubals, Nathalie</creatorcontrib><creatorcontrib>Dumas, Thomas</creatorcontrib><creatorcontrib>Guilbaud, Philippe</creatorcontrib><creatorcontrib>Martinez, Diego Moreno</creatorcontrib><creatorcontrib>Moisy, Philippe</creatorcontrib><creatorcontrib>Sauge-Merle, Sandrine</creatorcontrib><creatorcontrib>Lemaire, David</creatorcontrib><creatorcontrib>Solari, Pier Lorenzo</creatorcontrib><creatorcontrib>Berthon, Laurence</creatorcontrib><creatorcontrib>Berthomieu, Catherine</creatorcontrib><title>Investigation of the Plutonium(IV) Interactions with Two Variants of the EF-Hand Ca-Binding Site I of Calmodulin</title><title>Inorganic chemistry</title><addtitle>Inorg. Chem</addtitle><description>Due to its presence in the nuclear industry and its strong radiotoxicity, plutonium is an actinide of major interest in the event of internal contamination. To improve the understanding of its mechanisms of transport and accumulation in the body, the complexation of Pu(IV) to the most common protein calcium-binding motif found in cells, the EF-hand motif of calmodulin, was investigated. Visible and X-ray absorption spectroscopies (XAS) in solution made it possible to investigate the speciation of plutonium at physiological pH (pH 7.4) and pH 6 in two variants of the calmodulin Ca-binding site I and using Pu(IV) in different media: carbonate, chloride, or nitrate solutions. Three different species of Pu were identified in the samples, with formation of 1:1 Pu(IV):calmodulin peptide complexes, Pu(IV) reduction, and formation of peptide-mediated Pu(IV) hexanuclear cluster.</description><subject>Binding Sites</subject><subject>Calcium</subject><subject>Calmodulin</subject><subject>Life Sciences</subject><subject>Oxidation-Reduction</subject><subject>Plutonium - chemistry</subject><issn>0020-1669</issn><issn>1520-510X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFv2yAUhdG0qc3a_oRNPLYPzi7GYPzYRe1iKdImrav6hrCNEyobMsCt-u-HlTSve-IKfefcq3MQ-kJgSSAn31QblsY6v213elzSFkAU7ANaEJZDxgg8fUQLgDQTzqtz9DmEZwCoaMHP0DktiSgoLxZoX9sXHaLZqmicxa7Hcafxr2GKzpppvK4fb3Bto_aqnYGAX03c4YdXhx-VN8rG8K65u8_WynZ4pbLvxnbGbvFvEzWuZ2ClhtF102DsJfrUqyHoq-N7gf7c3z2s1tnm5496dbvJFKU0ZrnuK0Jyxhm0PS9zSIcL2oieEUZJrtuCasVFU3W64iVUXSf6pqGcaU1UXnb0At0cfHdqkHtvRuXfpFNGrm83cv6DIuVYVuyFJPb6wO69-zulOORoQquHQVntpiBzQagQFdAyoeyAtt6F4HV_8iYg52JkKkaeipHHYpLu63HF1Iy6O6nem0gAOQCz_tlN3qZ0_mP6D5OynI0</recordid><startdate>20230529</startdate><enddate>20230529</enddate><creator>Daronnat, Loïc</creator><creator>Holfeltz, Vanessa</creator><creator>Boubals, Nathalie</creator><creator>Dumas, Thomas</creator><creator>Guilbaud, Philippe</creator><creator>Martinez, Diego Moreno</creator><creator>Moisy, Philippe</creator><creator>Sauge-Merle, Sandrine</creator><creator>Lemaire, David</creator><creator>Solari, Pier Lorenzo</creator><creator>Berthon, Laurence</creator><creator>Berthomieu, Catherine</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-3474-8474</orcidid><orcidid>https://orcid.org/0000-0001-6425-6484</orcidid><orcidid>https://orcid.org/0000-0002-9331-0846</orcidid><orcidid>https://orcid.org/0000-0001-9777-8326</orcidid><orcidid>https://orcid.org/0000-0001-8784-5396</orcidid><orcidid>https://orcid.org/0000-0003-2721-3885</orcidid></search><sort><creationdate>20230529</creationdate><title>Investigation of the Plutonium(IV) Interactions with Two Variants of the EF-Hand Ca-Binding Site I of Calmodulin</title><author>Daronnat, Loïc ; Holfeltz, Vanessa ; Boubals, Nathalie ; Dumas, Thomas ; Guilbaud, Philippe ; Martinez, Diego Moreno ; Moisy, Philippe ; Sauge-Merle, Sandrine ; Lemaire, David ; Solari, Pier Lorenzo ; Berthon, Laurence ; Berthomieu, Catherine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a333t-2ef91125650cf672034683b8f515312ec43ea68b9de96709dd8fbb365ee1a27d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Binding Sites</topic><topic>Calcium</topic><topic>Calmodulin</topic><topic>Life Sciences</topic><topic>Oxidation-Reduction</topic><topic>Plutonium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Daronnat, Loïc</creatorcontrib><creatorcontrib>Holfeltz, Vanessa</creatorcontrib><creatorcontrib>Boubals, Nathalie</creatorcontrib><creatorcontrib>Dumas, Thomas</creatorcontrib><creatorcontrib>Guilbaud, Philippe</creatorcontrib><creatorcontrib>Martinez, Diego Moreno</creatorcontrib><creatorcontrib>Moisy, Philippe</creatorcontrib><creatorcontrib>Sauge-Merle, Sandrine</creatorcontrib><creatorcontrib>Lemaire, David</creatorcontrib><creatorcontrib>Solari, Pier Lorenzo</creatorcontrib><creatorcontrib>Berthon, Laurence</creatorcontrib><creatorcontrib>Berthomieu, Catherine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Inorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daronnat, Loïc</au><au>Holfeltz, Vanessa</au><au>Boubals, Nathalie</au><au>Dumas, Thomas</au><au>Guilbaud, Philippe</au><au>Martinez, Diego Moreno</au><au>Moisy, Philippe</au><au>Sauge-Merle, Sandrine</au><au>Lemaire, David</au><au>Solari, Pier Lorenzo</au><au>Berthon, Laurence</au><au>Berthomieu, Catherine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of the Plutonium(IV) Interactions with Two Variants of the EF-Hand Ca-Binding Site I of Calmodulin</atitle><jtitle>Inorganic chemistry</jtitle><addtitle>Inorg. Chem</addtitle><date>2023-05-29</date><risdate>2023</risdate><volume>62</volume><issue>21</issue><spage>8334</spage><epage>8346</epage><pages>8334-8346</pages><issn>0020-1669</issn><eissn>1520-510X</eissn><abstract>Due to its presence in the nuclear industry and its strong radiotoxicity, plutonium is an actinide of major interest in the event of internal contamination. To improve the understanding of its mechanisms of transport and accumulation in the body, the complexation of Pu(IV) to the most common protein calcium-binding motif found in cells, the EF-hand motif of calmodulin, was investigated. Visible and X-ray absorption spectroscopies (XAS) in solution made it possible to investigate the speciation of plutonium at physiological pH (pH 7.4) and pH 6 in two variants of the calmodulin Ca-binding site I and using Pu(IV) in different media: carbonate, chloride, or nitrate solutions. Three different species of Pu were identified in the samples, with formation of 1:1 Pu(IV):calmodulin peptide complexes, Pu(IV) reduction, and formation of peptide-mediated Pu(IV) hexanuclear cluster.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>37184364</pmid><doi>10.1021/acs.inorgchem.3c00845</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-3474-8474</orcidid><orcidid>https://orcid.org/0000-0001-6425-6484</orcidid><orcidid>https://orcid.org/0000-0002-9331-0846</orcidid><orcidid>https://orcid.org/0000-0001-9777-8326</orcidid><orcidid>https://orcid.org/0000-0001-8784-5396</orcidid><orcidid>https://orcid.org/0000-0003-2721-3885</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0020-1669 |
| ispartof | Inorganic chemistry, 2023-05, Vol.62 (21), p.8334-8346 |
| issn | 0020-1669 1520-510X |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_04102795v1 |
| source | ACS Publications; MEDLINE |
| subjects | Binding Sites Calcium Calmodulin Life Sciences Oxidation-Reduction Plutonium - chemistry |
| title | Investigation of the Plutonium(IV) Interactions with Two Variants of the EF-Hand Ca-Binding Site I of Calmodulin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T20%3A47%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Investigation%20of%20the%20Plutonium(IV)%20Interactions%20with%20Two%20Variants%20of%20the%20EF-Hand%20Ca-Binding%20Site%20I%20of%20Calmodulin&rft.jtitle=Inorganic%20chemistry&rft.au=Daronnat,%20Loi%CC%88c&rft.date=2023-05-29&rft.volume=62&rft.issue=21&rft.spage=8334&rft.epage=8346&rft.pages=8334-8346&rft.issn=0020-1669&rft.eissn=1520-510X&rft_id=info:doi/10.1021/acs.inorgchem.3c00845&rft_dat=%3Cproquest_hal_p%3E2813889037%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2813889037&rft_id=info:pmid/37184364&rfr_iscdi=true |