Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons
Recently, we and others reported that the doublecortin gene is responsible for X-linked lissencephaly and subcortical laminar heterotopia. Here, we show that Doublecortin is expressed in the brain throughout the period of corticogenesis in migrating and differentiating neurons. Immunohistochemical s...
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| Veröffentlicht in: | Neuron (Cambridge, Mass.) Mass.), 1999-06, Vol.23 (2), p.247-256 |
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| creator | Francis, F Koulakoff, A Boucher, D Chafey, P Schaar, B Vinet, M C Friocourt, G McDonnell, N Reiner, O Kahn, A McConnell, S K Berwald-Netter, Y Denoulet, P Chelly, J |
| description | Recently, we and others reported that the doublecortin gene is responsible for X-linked lissencephaly and subcortical laminar heterotopia. Here, we show that Doublecortin is expressed in the brain throughout the period of corticogenesis in migrating and differentiating neurons. Immunohistochemical studies show its localization in the soma and leading processes of tangentially migrating neurons, and a strong axonal labeling is observed in differentiating neurons. In cultured neurons, Doublecortin expression is highest in the distal parts of developing processes. We demonstrate by sedimentation and microscopy studies that Doublecortin is associated with microtubules (MTs) and postulate that it is a novel MAP. Our data suggest that the cortical dysgeneses associated with the loss of Doublecortin function might result from abnormal cytoskeletal dynamics in neuronal cell development. |
| doi_str_mv | 10.1016/s0896-6273(00)80777-1 |
| format | Article |
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Our data suggest that the cortical dysgeneses associated with the loss of Doublecortin function might result from abnormal cytoskeletal dynamics in neuronal cell development.</description><identifier>ISSN: 0896-6273</identifier><identifier>DOI: 10.1016/s0896-6273(00)80777-1</identifier><identifier>PMID: 10399932</identifier><language>eng</language><publisher>United States: Elsevier</publisher><subject>Animals ; Antibody Specificity ; Biochemistry, Molecular Biology ; Brain - cytology ; Brain - embryology ; Brain - metabolism ; Cell Differentiation - physiology ; Cell Movement - physiology ; Cells, Cultured ; Cytoskeleton - metabolism ; Cytoskeleton - ultrastructure ; Genetics ; Immunohistochemistry ; In Situ Hybridization ; Life Sciences ; Mice ; Microtubule-Associated Proteins - biosynthesis ; Microtubule-Associated Proteins - physiology ; Neurons - metabolism ; Neurons - physiology ; Neurons - ultrastructure ; Neurons and Cognition ; Neuropeptides - biosynthesis ; Neuropeptides - physiology ; Phosphoproteins - biosynthesis ; Phosphoproteins - physiology ; Rats ; Rats, Long-Evans ; RNA, Messenger - biosynthesis ; Tubulin - isolation & purification ; Tubulin - metabolism</subject><ispartof>Neuron (Cambridge, Mass.), 1999-06, Vol.23 (2), p.247-256</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c535t-6a348038917f6e8d97bcb2b7c5baf642db93f22316552cc773fdbcfdfa029cb93</citedby><cites>FETCH-LOGICAL-c535t-6a348038917f6e8d97bcb2b7c5baf642db93f22316552cc773fdbcfdfa029cb93</cites><orcidid>0000-0001-6681-7644</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10399932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04016864$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Francis, F</creatorcontrib><creatorcontrib>Koulakoff, A</creatorcontrib><creatorcontrib>Boucher, D</creatorcontrib><creatorcontrib>Chafey, P</creatorcontrib><creatorcontrib>Schaar, B</creatorcontrib><creatorcontrib>Vinet, M C</creatorcontrib><creatorcontrib>Friocourt, G</creatorcontrib><creatorcontrib>McDonnell, N</creatorcontrib><creatorcontrib>Reiner, O</creatorcontrib><creatorcontrib>Kahn, A</creatorcontrib><creatorcontrib>McConnell, S K</creatorcontrib><creatorcontrib>Berwald-Netter, Y</creatorcontrib><creatorcontrib>Denoulet, P</creatorcontrib><creatorcontrib>Chelly, J</creatorcontrib><title>Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons</title><title>Neuron (Cambridge, Mass.)</title><addtitle>Neuron</addtitle><description>Recently, we and others reported that the doublecortin gene is responsible for X-linked lissencephaly and subcortical laminar heterotopia. 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Koulakoff, A ; Boucher, D ; Chafey, P ; Schaar, B ; Vinet, M C ; Friocourt, G ; McDonnell, N ; Reiner, O ; Kahn, A ; McConnell, S K ; Berwald-Netter, Y ; Denoulet, P ; Chelly, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c535t-6a348038917f6e8d97bcb2b7c5baf642db93f22316552cc773fdbcfdfa029cb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Biochemistry, Molecular Biology</topic><topic>Brain - cytology</topic><topic>Brain - embryology</topic><topic>Brain - metabolism</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Movement - physiology</topic><topic>Cells, Cultured</topic><topic>Cytoskeleton - metabolism</topic><topic>Cytoskeleton - ultrastructure</topic><topic>Genetics</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Microtubule-Associated Proteins - biosynthesis</topic><topic>Microtubule-Associated Proteins - physiology</topic><topic>Neurons - metabolism</topic><topic>Neurons - physiology</topic><topic>Neurons - ultrastructure</topic><topic>Neurons and Cognition</topic><topic>Neuropeptides - biosynthesis</topic><topic>Neuropeptides - physiology</topic><topic>Phosphoproteins - biosynthesis</topic><topic>Phosphoproteins - physiology</topic><topic>Rats</topic><topic>Rats, Long-Evans</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Tubulin - isolation & purification</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Francis, F</creatorcontrib><creatorcontrib>Koulakoff, A</creatorcontrib><creatorcontrib>Boucher, D</creatorcontrib><creatorcontrib>Chafey, P</creatorcontrib><creatorcontrib>Schaar, B</creatorcontrib><creatorcontrib>Vinet, M C</creatorcontrib><creatorcontrib>Friocourt, G</creatorcontrib><creatorcontrib>McDonnell, N</creatorcontrib><creatorcontrib>Reiner, O</creatorcontrib><creatorcontrib>Kahn, A</creatorcontrib><creatorcontrib>McConnell, S K</creatorcontrib><creatorcontrib>Berwald-Netter, Y</creatorcontrib><creatorcontrib>Denoulet, P</creatorcontrib><creatorcontrib>Chelly, J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Neuron (Cambridge, Mass.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Francis, F</au><au>Koulakoff, A</au><au>Boucher, D</au><au>Chafey, P</au><au>Schaar, B</au><au>Vinet, M C</au><au>Friocourt, G</au><au>McDonnell, N</au><au>Reiner, O</au><au>Kahn, A</au><au>McConnell, S K</au><au>Berwald-Netter, Y</au><au>Denoulet, P</au><au>Chelly, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons</atitle><jtitle>Neuron (Cambridge, Mass.)</jtitle><addtitle>Neuron</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>23</volume><issue>2</issue><spage>247</spage><epage>256</epage><pages>247-256</pages><issn>0896-6273</issn><abstract>Recently, we and others reported that the doublecortin gene is responsible for X-linked lissencephaly and subcortical laminar heterotopia. 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| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibody Specificity Biochemistry, Molecular Biology Brain - cytology Brain - embryology Brain - metabolism Cell Differentiation - physiology Cell Movement - physiology Cells, Cultured Cytoskeleton - metabolism Cytoskeleton - ultrastructure Genetics Immunohistochemistry In Situ Hybridization Life Sciences Mice Microtubule-Associated Proteins - biosynthesis Microtubule-Associated Proteins - physiology Neurons - metabolism Neurons - physiology Neurons - ultrastructure Neurons and Cognition Neuropeptides - biosynthesis Neuropeptides - physiology Phosphoproteins - biosynthesis Phosphoproteins - physiology Rats Rats, Long-Evans RNA, Messenger - biosynthesis Tubulin - isolation & purification Tubulin - metabolism |
| title | Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons |
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