Regulation of Interleukin‑6 in Head and Neck Squamous Cell Carcinoma Is Related to Papillomavirus Infection
The prevalence of head and neck squamous cell carcinoma (HNSCC) related to human papillomavirus (HPV) is increasing, unlike tobacco- and alcohol-associated cancers. To gain a clearer understanding of the molecular mechanisms implicated in HNSCC, depending on the presence or not of a viral sequence,...
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| Veröffentlicht in: | Journal of proteome research 2014-02, Vol.13 (2), p.1002-1011 |
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| creator | Guerrera, Ida Chiara Quetier, Ivan Fetouchi, Rachid Moreau, Frederique Vauloup-Fellous, Christelle Lekbaby, Bouchra Rousselot, Caroline Chhuon, Cerina Edelman, Aleksander Lefevre, Marine Nicolas, Jean-Claude Kremsdorf, Dina Lacau Saint Guily, Jean Soussan, Patrick |
| description | The prevalence of head and neck squamous cell carcinoma (HNSCC) related to human papillomavirus (HPV) is increasing, unlike tobacco- and alcohol-associated cancers. To gain a clearer understanding of the molecular mechanisms implicated in HNSCC, depending on the presence or not of a viral sequence, we investigated the expression of proteins detected in the tumor regions of HNSCC patients. Twenty-two untreated HNSCC patients were selected according to the presence of HPV-16. For six patients, tumor and controlateral healthy tissues were tested for viral detection before quantitative proteomic analysis. After confirmation by Western blot, proteins were connected into a network, leading to investigate interleukin-6 (IL-6) by immunocytochemistry and ELISA. 41 ± 5% of proteins quantified by proteomics were differentially expressed in tumor compared with healthy regions. Among them, 36 proteins were retained as modulated in HPV-16 positive or negative tumors, including cytokeratins, tubulins, annexin A1, and serpin B1. Network analysis suggested a central role of IL-6, confirmed by overexpression of IL-6 in tumor tissues as in sera of HPV-negative HNSCC compared with HPV-16-positive tumors. This modulation may contribute to the survival and proliferation of cancer cells, although it was not related to tumor stage or to the level of HPV-16 DNA. |
| doi_str_mv | 10.1021/pr401009f |
| format | Article |
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To gain a clearer understanding of the molecular mechanisms implicated in HNSCC, depending on the presence or not of a viral sequence, we investigated the expression of proteins detected in the tumor regions of HNSCC patients. Twenty-two untreated HNSCC patients were selected according to the presence of HPV-16. For six patients, tumor and controlateral healthy tissues were tested for viral detection before quantitative proteomic analysis. After confirmation by Western blot, proteins were connected into a network, leading to investigate interleukin-6 (IL-6) by immunocytochemistry and ELISA. 41 ± 5% of proteins quantified by proteomics were differentially expressed in tumor compared with healthy regions. Among them, 36 proteins were retained as modulated in HPV-16 positive or negative tumors, including cytokeratins, tubulins, annexin A1, and serpin B1. Network analysis suggested a central role of IL-6, confirmed by overexpression of IL-6 in tumor tissues as in sera of HPV-negative HNSCC compared with HPV-16-positive tumors. This modulation may contribute to the survival and proliferation of cancer cells, although it was not related to tumor stage or to the level of HPV-16 DNA.</description><identifier>ISSN: 1535-3893</identifier><identifier>EISSN: 1535-3907</identifier><identifier>DOI: 10.1021/pr401009f</identifier><identifier>PMID: 24405156</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Aged ; Base Sequence ; Blotting, Western ; Cancer ; Carcinoma, Squamous Cell - complications ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - virology ; Case-Control Studies ; Cohort Studies ; DNA Primers ; Enzyme-Linked Immunosorbent Assay ; Female ; Head and Neck Neoplasms - complications ; Head and Neck Neoplasms - metabolism ; Head and Neck Neoplasms - virology ; Human papillomavirus 16 - isolation & purification ; Humans ; Interleukin-6 - metabolism ; Life Sciences ; Male ; Middle Aged ; Papillomavirus Infections - complications ; Papillomavirus Infections - metabolism ; Papillomavirus Infections - virology ; Real-Time Polymerase Chain Reaction ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Viral Load</subject><ispartof>Journal of proteome research, 2014-02, Vol.13 (2), p.1002-1011</ispartof><rights>Copyright © 2014 American Chemical Society</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a349t-41744cbc2bfca640d18df2421e32c97a292b1cb6494f91111d1f1924acb8c4cd3</citedby><cites>FETCH-LOGICAL-a349t-41744cbc2bfca640d18df2421e32c97a292b1cb6494f91111d1f1924acb8c4cd3</cites><orcidid>0000-0002-4832-6793 ; 0000-0003-2706-7371 ; 0000-0003-1347-7492 ; 0000-0001-6627-4264</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/pr401009f$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/pr401009f$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,780,784,885,2756,27067,27915,27916,56729,56779</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24405156$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-04012942$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Guerrera, Ida Chiara</creatorcontrib><creatorcontrib>Quetier, Ivan</creatorcontrib><creatorcontrib>Fetouchi, Rachid</creatorcontrib><creatorcontrib>Moreau, Frederique</creatorcontrib><creatorcontrib>Vauloup-Fellous, Christelle</creatorcontrib><creatorcontrib>Lekbaby, Bouchra</creatorcontrib><creatorcontrib>Rousselot, Caroline</creatorcontrib><creatorcontrib>Chhuon, Cerina</creatorcontrib><creatorcontrib>Edelman, Aleksander</creatorcontrib><creatorcontrib>Lefevre, Marine</creatorcontrib><creatorcontrib>Nicolas, Jean-Claude</creatorcontrib><creatorcontrib>Kremsdorf, Dina</creatorcontrib><creatorcontrib>Lacau Saint Guily, Jean</creatorcontrib><creatorcontrib>Soussan, Patrick</creatorcontrib><title>Regulation of Interleukin‑6 in Head and Neck Squamous Cell Carcinoma Is Related to Papillomavirus Infection</title><title>Journal of proteome research</title><addtitle>J. Proteome Res</addtitle><description>The prevalence of head and neck squamous cell carcinoma (HNSCC) related to human papillomavirus (HPV) is increasing, unlike tobacco- and alcohol-associated cancers. To gain a clearer understanding of the molecular mechanisms implicated in HNSCC, depending on the presence or not of a viral sequence, we investigated the expression of proteins detected in the tumor regions of HNSCC patients. Twenty-two untreated HNSCC patients were selected according to the presence of HPV-16. For six patients, tumor and controlateral healthy tissues were tested for viral detection before quantitative proteomic analysis. After confirmation by Western blot, proteins were connected into a network, leading to investigate interleukin-6 (IL-6) by immunocytochemistry and ELISA. 41 ± 5% of proteins quantified by proteomics were differentially expressed in tumor compared with healthy regions. Among them, 36 proteins were retained as modulated in HPV-16 positive or negative tumors, including cytokeratins, tubulins, annexin A1, and serpin B1. Network analysis suggested a central role of IL-6, confirmed by overexpression of IL-6 in tumor tissues as in sera of HPV-negative HNSCC compared with HPV-16-positive tumors. This modulation may contribute to the survival and proliferation of cancer cells, although it was not related to tumor stage or to the level of HPV-16 DNA.</description><subject>Aged</subject><subject>Base Sequence</subject><subject>Blotting, Western</subject><subject>Cancer</subject><subject>Carcinoma, Squamous Cell - complications</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - virology</subject><subject>Case-Control Studies</subject><subject>Cohort Studies</subject><subject>DNA Primers</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Head and Neck Neoplasms - complications</subject><subject>Head and Neck Neoplasms - metabolism</subject><subject>Head and Neck Neoplasms - virology</subject><subject>Human papillomavirus 16 - isolation & purification</subject><subject>Humans</subject><subject>Interleukin-6 - metabolism</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Papillomavirus Infections - complications</subject><subject>Papillomavirus Infections - metabolism</subject><subject>Papillomavirus Infections - virology</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Viral Load</subject><issn>1535-3893</issn><issn>1535-3907</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkcFOGzEQhq2qqKG0B14A-YJEDwGP15uNjyiCJlLUVqE9W7NeGwy7drB3I3HrK_QVeRIcBcKlvow1-vSN5h9CjoGdA-NwsY6CAWPSfiCHUBbluJCs-vj2n8piRD6ndM8YlBUrPpERF4KVUE4OSbcyt0OLvQueBksXvjexNcOD889__02o83RusKHoG_rD6Ad68zhgF4ZEZ6Zt6Qyjdj50SBeJrkz2mIb2gf7CtWvb3N-4mNmFt0ZvR3whBxbbZL6-1iPy5_rq92w-Xv78vphdLsdYCNmPBVRC6Frz2mqcCNbAtLFccDAF17JCLnkNup4IKayE_BqwILlAXU-10E1xRL7tvHfYqnV0HcYnFdCp-eVSbXssB8al4BvI7NmOXcfwOJjUq84lnbdDb_KiCoSUUEAJ1btWx5BSNHbvBqa2l1D7S2T25FU71J1p9uRb9Bk43QGok7oPQ_Q5kf-IXgADyI9a</recordid><startdate>20140207</startdate><enddate>20140207</enddate><creator>Guerrera, Ida Chiara</creator><creator>Quetier, Ivan</creator><creator>Fetouchi, Rachid</creator><creator>Moreau, Frederique</creator><creator>Vauloup-Fellous, Christelle</creator><creator>Lekbaby, Bouchra</creator><creator>Rousselot, Caroline</creator><creator>Chhuon, Cerina</creator><creator>Edelman, Aleksander</creator><creator>Lefevre, Marine</creator><creator>Nicolas, Jean-Claude</creator><creator>Kremsdorf, Dina</creator><creator>Lacau Saint Guily, Jean</creator><creator>Soussan, Patrick</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4832-6793</orcidid><orcidid>https://orcid.org/0000-0003-2706-7371</orcidid><orcidid>https://orcid.org/0000-0003-1347-7492</orcidid><orcidid>https://orcid.org/0000-0001-6627-4264</orcidid></search><sort><creationdate>20140207</creationdate><title>Regulation of Interleukin‑6 in Head and Neck Squamous Cell Carcinoma Is Related to Papillomavirus Infection</title><author>Guerrera, Ida Chiara ; Quetier, Ivan ; Fetouchi, Rachid ; Moreau, Frederique ; Vauloup-Fellous, Christelle ; Lekbaby, Bouchra ; Rousselot, Caroline ; Chhuon, Cerina ; Edelman, Aleksander ; Lefevre, Marine ; Nicolas, Jean-Claude ; Kremsdorf, Dina ; Lacau Saint Guily, Jean ; Soussan, Patrick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a349t-41744cbc2bfca640d18df2421e32c97a292b1cb6494f91111d1f1924acb8c4cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aged</topic><topic>Base Sequence</topic><topic>Blotting, Western</topic><topic>Cancer</topic><topic>Carcinoma, Squamous Cell - complications</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - virology</topic><topic>Case-Control Studies</topic><topic>Cohort Studies</topic><topic>DNA Primers</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Head and Neck Neoplasms - complications</topic><topic>Head and Neck Neoplasms - metabolism</topic><topic>Head and Neck Neoplasms - virology</topic><topic>Human papillomavirus 16 - isolation & purification</topic><topic>Humans</topic><topic>Interleukin-6 - metabolism</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Papillomavirus Infections - complications</topic><topic>Papillomavirus Infections - metabolism</topic><topic>Papillomavirus Infections - virology</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Viral Load</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guerrera, Ida Chiara</creatorcontrib><creatorcontrib>Quetier, Ivan</creatorcontrib><creatorcontrib>Fetouchi, Rachid</creatorcontrib><creatorcontrib>Moreau, Frederique</creatorcontrib><creatorcontrib>Vauloup-Fellous, Christelle</creatorcontrib><creatorcontrib>Lekbaby, Bouchra</creatorcontrib><creatorcontrib>Rousselot, Caroline</creatorcontrib><creatorcontrib>Chhuon, Cerina</creatorcontrib><creatorcontrib>Edelman, Aleksander</creatorcontrib><creatorcontrib>Lefevre, Marine</creatorcontrib><creatorcontrib>Nicolas, Jean-Claude</creatorcontrib><creatorcontrib>Kremsdorf, Dina</creatorcontrib><creatorcontrib>Lacau Saint Guily, Jean</creatorcontrib><creatorcontrib>Soussan, Patrick</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of proteome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guerrera, Ida Chiara</au><au>Quetier, Ivan</au><au>Fetouchi, Rachid</au><au>Moreau, Frederique</au><au>Vauloup-Fellous, Christelle</au><au>Lekbaby, Bouchra</au><au>Rousselot, Caroline</au><au>Chhuon, Cerina</au><au>Edelman, Aleksander</au><au>Lefevre, Marine</au><au>Nicolas, Jean-Claude</au><au>Kremsdorf, Dina</au><au>Lacau Saint Guily, Jean</au><au>Soussan, Patrick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Interleukin‑6 in Head and Neck Squamous Cell Carcinoma Is Related to Papillomavirus Infection</atitle><jtitle>Journal of proteome research</jtitle><addtitle>J. Proteome Res</addtitle><date>2014-02-07</date><risdate>2014</risdate><volume>13</volume><issue>2</issue><spage>1002</spage><epage>1011</epage><pages>1002-1011</pages><issn>1535-3893</issn><eissn>1535-3907</eissn><abstract>The prevalence of head and neck squamous cell carcinoma (HNSCC) related to human papillomavirus (HPV) is increasing, unlike tobacco- and alcohol-associated cancers. To gain a clearer understanding of the molecular mechanisms implicated in HNSCC, depending on the presence or not of a viral sequence, we investigated the expression of proteins detected in the tumor regions of HNSCC patients. Twenty-two untreated HNSCC patients were selected according to the presence of HPV-16. For six patients, tumor and controlateral healthy tissues were tested for viral detection before quantitative proteomic analysis. After confirmation by Western blot, proteins were connected into a network, leading to investigate interleukin-6 (IL-6) by immunocytochemistry and ELISA. 41 ± 5% of proteins quantified by proteomics were differentially expressed in tumor compared with healthy regions. Among them, 36 proteins were retained as modulated in HPV-16 positive or negative tumors, including cytokeratins, tubulins, annexin A1, and serpin B1. Network analysis suggested a central role of IL-6, confirmed by overexpression of IL-6 in tumor tissues as in sera of HPV-negative HNSCC compared with HPV-16-positive tumors. This modulation may contribute to the survival and proliferation of cancer cells, although it was not related to tumor stage or to the level of HPV-16 DNA.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24405156</pmid><doi>10.1021/pr401009f</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-4832-6793</orcidid><orcidid>https://orcid.org/0000-0003-2706-7371</orcidid><orcidid>https://orcid.org/0000-0003-1347-7492</orcidid><orcidid>https://orcid.org/0000-0001-6627-4264</orcidid></addata></record> |
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| ispartof | Journal of proteome research, 2014-02, Vol.13 (2), p.1002-1011 |
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| subjects | Aged Base Sequence Blotting, Western Cancer Carcinoma, Squamous Cell - complications Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - virology Case-Control Studies Cohort Studies DNA Primers Enzyme-Linked Immunosorbent Assay Female Head and Neck Neoplasms - complications Head and Neck Neoplasms - metabolism Head and Neck Neoplasms - virology Human papillomavirus 16 - isolation & purification Humans Interleukin-6 - metabolism Life Sciences Male Middle Aged Papillomavirus Infections - complications Papillomavirus Infections - metabolism Papillomavirus Infections - virology Real-Time Polymerase Chain Reaction Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Viral Load |
| title | Regulation of Interleukin‑6 in Head and Neck Squamous Cell Carcinoma Is Related to Papillomavirus Infection |
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