Inhibition of cardiac baroreflex by noxious thermal stimuli: A key role for lateral paragigantocellular serotonergic cells
The present study was designed to identify the neuronal mechanisms causing cardiac baroreflex inhibition associated with thermal nociception in rats. Under urethane-anesthesia, noxious thermal stimuli ⩾48 °C were found to inhibit the cardiac baroreflex, whereas noxious stimuli ⩽46 °C had no effect....
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| Veröffentlicht in: | Pain (Amsterdam) 2009-12, Vol.146 (3), p.315-324 |
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| description | The present study was designed to identify the neuronal mechanisms causing cardiac baroreflex inhibition associated with thermal nociception in rats. Under urethane-anesthesia, noxious thermal stimuli ⩾48
°C were found to inhibit the cardiac baroreflex, whereas noxious stimuli ⩽46
°C had no effect. Using double immunohistochemical labeling, noxious stimuli ⩾48
°C were found to evoke primarily a strong expression of Fos protein (Fos) encoded by
c-fos gene in serotonergic neurons of lateral paragigantocellular reticular nucleus (LPGi). Noxious stimuli ⩽46
°C did not evoke Fos expression in any serotonergic neurons of the brainstem. Local blockade of neuronal activity by bilateral microinjections of fluorescent muscimol (a GABA
A receptor agonist tagged with a fluorophore that allowed visualization of the injections) into both the LPGi and the raphe magnus nucleus prevented the inhibitory effect of noxious stimuli ⩾48
°C on the cardiac baroreflex. Bilateral microinjections of granisetron (a 5-HT
3 antagonist) within the nucleus tractus solitarius also prevented the inhibition of cardiac baroreflex elicited by noxious stimuli ⩾48
°C. These results show that activation of serotonergic cells in the LPGi is critical to trigger nucleus tractus solitarius-mediated cardiac baroreflex inhibition elicited by intense thermal noxious stimuli. |
| doi_str_mv | 10.1016/j.pain.2009.09.018 |
| format | Article |
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°C were found to inhibit the cardiac baroreflex, whereas noxious stimuli ⩽46
°C had no effect. Using double immunohistochemical labeling, noxious stimuli ⩾48
°C were found to evoke primarily a strong expression of Fos protein (Fos) encoded by
c-fos gene in serotonergic neurons of lateral paragigantocellular reticular nucleus (LPGi). Noxious stimuli ⩽46
°C did not evoke Fos expression in any serotonergic neurons of the brainstem. Local blockade of neuronal activity by bilateral microinjections of fluorescent muscimol (a GABA
A receptor agonist tagged with a fluorophore that allowed visualization of the injections) into both the LPGi and the raphe magnus nucleus prevented the inhibitory effect of noxious stimuli ⩾48
°C on the cardiac baroreflex. Bilateral microinjections of granisetron (a 5-HT
3 antagonist) within the nucleus tractus solitarius also prevented the inhibition of cardiac baroreflex elicited by noxious stimuli ⩾48
°C. These results show that activation of serotonergic cells in the LPGi is critical to trigger nucleus tractus solitarius-mediated cardiac baroreflex inhibition elicited by intense thermal noxious stimuli.</description><identifier>ISSN: 0304-3959</identifier><identifier>EISSN: 1872-6623</identifier><identifier>DOI: 10.1016/j.pain.2009.09.018</identifier><identifier>PMID: 19879048</identifier><identifier>CODEN: PAINDB</identifier><language>eng</language><publisher>Philadelphia, PA: Elsevier B.V</publisher><subject>Animals ; Baroreflex - physiology ; Biological and medical sciences ; c-Fos ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; GABA Agonists - pharmacology ; Genes, fos ; Granisetron - administration & dosage ; Granisetron - pharmacology ; Heart - physiopathology ; Heart rate ; Hot Temperature ; Immunohistochemistry ; Life Sciences ; Male ; Medulla Oblongata - physiopathology ; Microinjections ; Muscimol - pharmacology ; Pain ; Pain - physiopathology ; Perception ; Proprioception. Interoception. Pain perception ; Psychology. Psychoanalysis. Psychiatry ; Psychology. Psychophysiology ; Raphe magnus ; Raphe Nuclei - physiopathology ; Rats ; Rats, Sprague-Dawley ; Serotonin ; Serotonin - physiology ; Serotonin Antagonists - administration & dosage ; Serotonin Antagonists - pharmacology ; Solitary Nucleus - physiopathology ; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors ; Tractus solitarius ; Ventromedial medulla ; Vertebrates: nervous system and sense organs</subject><ispartof>Pain (Amsterdam), 2009-12, Vol.146 (3), p.315-324</ispartof><rights>2009 International Association for the Study of Pain</rights><rights>Lippincott Williams & Wilkins, Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5307-93ffe1a679072bff5fc3d28c3226e0055e6bebb680ffed32e5a1859b1a397a4b3</citedby><cites>FETCH-LOGICAL-c5307-93ffe1a679072bff5fc3d28c3226e0055e6bebb680ffed32e5a1859b1a397a4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22177656$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19879048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03988109$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Gau, Rémi</creatorcontrib><creatorcontrib>Sévoz-Couche, Caroline</creatorcontrib><creatorcontrib>Laguzzi, Raùl</creatorcontrib><creatorcontrib>Hamon, Michel</creatorcontrib><creatorcontrib>Bernard, Jean-François</creatorcontrib><title>Inhibition of cardiac baroreflex by noxious thermal stimuli: A key role for lateral paragigantocellular serotonergic cells</title><title>Pain (Amsterdam)</title><addtitle>Pain</addtitle><description>The present study was designed to identify the neuronal mechanisms causing cardiac baroreflex inhibition associated with thermal nociception in rats. Under urethane-anesthesia, noxious thermal stimuli ⩾48
°C were found to inhibit the cardiac baroreflex, whereas noxious stimuli ⩽46
°C had no effect. Using double immunohistochemical labeling, noxious stimuli ⩾48
°C were found to evoke primarily a strong expression of Fos protein (Fos) encoded by
c-fos gene in serotonergic neurons of lateral paragigantocellular reticular nucleus (LPGi). Noxious stimuli ⩽46
°C did not evoke Fos expression in any serotonergic neurons of the brainstem. Local blockade of neuronal activity by bilateral microinjections of fluorescent muscimol (a GABA
A receptor agonist tagged with a fluorophore that allowed visualization of the injections) into both the LPGi and the raphe magnus nucleus prevented the inhibitory effect of noxious stimuli ⩾48
°C on the cardiac baroreflex. Bilateral microinjections of granisetron (a 5-HT
3 antagonist) within the nucleus tractus solitarius also prevented the inhibition of cardiac baroreflex elicited by noxious stimuli ⩾48
°C. These results show that activation of serotonergic cells in the LPGi is critical to trigger nucleus tractus solitarius-mediated cardiac baroreflex inhibition elicited by intense thermal noxious stimuli.</description><subject>Animals</subject><subject>Baroreflex - physiology</subject><subject>Biological and medical sciences</subject><subject>c-Fos</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GABA Agonists - pharmacology</subject><subject>Genes, fos</subject><subject>Granisetron - administration & dosage</subject><subject>Granisetron - pharmacology</subject><subject>Heart - physiopathology</subject><subject>Heart rate</subject><subject>Hot Temperature</subject><subject>Immunohistochemistry</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medulla Oblongata - physiopathology</subject><subject>Microinjections</subject><subject>Muscimol - pharmacology</subject><subject>Pain</subject><subject>Pain - physiopathology</subject><subject>Perception</subject><subject>Proprioception. Interoception. Pain perception</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychology. Psychophysiology</subject><subject>Raphe magnus</subject><subject>Raphe Nuclei - physiopathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Serotonin</subject><subject>Serotonin - physiology</subject><subject>Serotonin Antagonists - administration & dosage</subject><subject>Serotonin Antagonists - pharmacology</subject><subject>Solitary Nucleus - physiopathology</subject><subject>Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors</subject><subject>Tractus solitarius</subject><subject>Ventromedial medulla</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3959</issn><issn>1872-6623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxSMEokvhC3BAviDEIYv_bBwbcVlV0FZaiQucrYkz2fXWGy920nb59DjKqr0hjWRp_Hszo_eK4j2jS0aZ_LJfHsH1S06pXk7F1ItiwVTNSym5eFksqKCrUuhKXxRvUtpTSjnn-nVxwbSqNV2pRfH3tt-5xg0u9CR0xEJsHVjSQAwRO4-PpDmRPjy6MCYy7DAewJM0uMPo3VeyJnd4IjF4JF2IxMOAMf8fIcLWbaEfgkXvRw-RJIxhCD3GrbNk6qa3xasOfMJ35_ey-P3j-6-rm3Lz8_r2ar0pbSVoXWrRdchA5oNr3nRd1VnRcmUF5xIprSqUDTaNVDRzreBYAVOVbhgIXcOqEZfF53nuDrw5RneAeDIBnLlZb8zUo0Irxai-Z5n9NLPHGP6MmAZzcGm6FnrMDpharJigWulM8pm0MaSUvXoazaiZ4jF7M8VjpnjMVExl0Yfz-LE5YPssOeeRgY9nAJIF30XorUtPHOesrmUlM7eauYfgs-fpzo8PGM0OwQ87k4OmUmhZTrsZpxUtc4fVWfZtlmH2-95lRbIOe4uti2gH0wb3v_P_AXDdv2E</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Gau, Rémi</creator><creator>Sévoz-Couche, Caroline</creator><creator>Laguzzi, Raùl</creator><creator>Hamon, Michel</creator><creator>Bernard, Jean-François</creator><general>Elsevier B.V</general><general>Lippincott Williams & Wilkins, Inc</general><general>Elsevier</general><general>Lippincott, Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20091201</creationdate><title>Inhibition of cardiac baroreflex by noxious thermal stimuli: A key role for lateral paragigantocellular serotonergic cells</title><author>Gau, Rémi ; Sévoz-Couche, Caroline ; Laguzzi, Raùl ; Hamon, Michel ; Bernard, Jean-François</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5307-93ffe1a679072bff5fc3d28c3226e0055e6bebb680ffed32e5a1859b1a397a4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Baroreflex - physiology</topic><topic>Biological and medical sciences</topic><topic>c-Fos</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GABA Agonists - pharmacology</topic><topic>Genes, fos</topic><topic>Granisetron - administration & dosage</topic><topic>Granisetron - pharmacology</topic><topic>Heart - physiopathology</topic><topic>Heart rate</topic><topic>Hot Temperature</topic><topic>Immunohistochemistry</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medulla Oblongata - physiopathology</topic><topic>Microinjections</topic><topic>Muscimol - pharmacology</topic><topic>Pain</topic><topic>Pain - physiopathology</topic><topic>Perception</topic><topic>Proprioception. Interoception. Pain perception</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychology. Psychophysiology</topic><topic>Raphe magnus</topic><topic>Raphe Nuclei - physiopathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Serotonin</topic><topic>Serotonin - physiology</topic><topic>Serotonin Antagonists - administration & dosage</topic><topic>Serotonin Antagonists - pharmacology</topic><topic>Solitary Nucleus - physiopathology</topic><topic>Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors</topic><topic>Tractus solitarius</topic><topic>Ventromedial medulla</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gau, Rémi</creatorcontrib><creatorcontrib>Sévoz-Couche, Caroline</creatorcontrib><creatorcontrib>Laguzzi, Raùl</creatorcontrib><creatorcontrib>Hamon, Michel</creatorcontrib><creatorcontrib>Bernard, Jean-François</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Pain (Amsterdam)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gau, Rémi</au><au>Sévoz-Couche, Caroline</au><au>Laguzzi, Raùl</au><au>Hamon, Michel</au><au>Bernard, Jean-François</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of cardiac baroreflex by noxious thermal stimuli: A key role for lateral paragigantocellular serotonergic cells</atitle><jtitle>Pain (Amsterdam)</jtitle><addtitle>Pain</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>146</volume><issue>3</issue><spage>315</spage><epage>324</epage><pages>315-324</pages><issn>0304-3959</issn><eissn>1872-6623</eissn><coden>PAINDB</coden><abstract>The present study was designed to identify the neuronal mechanisms causing cardiac baroreflex inhibition associated with thermal nociception in rats. Under urethane-anesthesia, noxious thermal stimuli ⩾48
°C were found to inhibit the cardiac baroreflex, whereas noxious stimuli ⩽46
°C had no effect. Using double immunohistochemical labeling, noxious stimuli ⩾48
°C were found to evoke primarily a strong expression of Fos protein (Fos) encoded by
c-fos gene in serotonergic neurons of lateral paragigantocellular reticular nucleus (LPGi). Noxious stimuli ⩽46
°C did not evoke Fos expression in any serotonergic neurons of the brainstem. Local blockade of neuronal activity by bilateral microinjections of fluorescent muscimol (a GABA
A receptor agonist tagged with a fluorophore that allowed visualization of the injections) into both the LPGi and the raphe magnus nucleus prevented the inhibitory effect of noxious stimuli ⩾48
°C on the cardiac baroreflex. Bilateral microinjections of granisetron (a 5-HT
3 antagonist) within the nucleus tractus solitarius also prevented the inhibition of cardiac baroreflex elicited by noxious stimuli ⩾48
°C. These results show that activation of serotonergic cells in the LPGi is critical to trigger nucleus tractus solitarius-mediated cardiac baroreflex inhibition elicited by intense thermal noxious stimuli.</abstract><cop>Philadelphia, PA</cop><pub>Elsevier B.V</pub><pmid>19879048</pmid><doi>10.1016/j.pain.2009.09.018</doi><tpages>10</tpages></addata></record> |
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| ispartof | Pain (Amsterdam), 2009-12, Vol.146 (3), p.315-324 |
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| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Baroreflex - physiology Biological and medical sciences c-Fos Fluorescent Dyes Fundamental and applied biological sciences. Psychology GABA Agonists - pharmacology Genes, fos Granisetron - administration & dosage Granisetron - pharmacology Heart - physiopathology Heart rate Hot Temperature Immunohistochemistry Life Sciences Male Medulla Oblongata - physiopathology Microinjections Muscimol - pharmacology Pain Pain - physiopathology Perception Proprioception. Interoception. Pain perception Psychology. Psychoanalysis. Psychiatry Psychology. Psychophysiology Raphe magnus Raphe Nuclei - physiopathology Rats Rats, Sprague-Dawley Serotonin Serotonin - physiology Serotonin Antagonists - administration & dosage Serotonin Antagonists - pharmacology Solitary Nucleus - physiopathology Somesthesis and somesthetic pathways (proprioception, exteroception, nociception) interoception electrolocation. Sensory receptors Tractus solitarius Ventromedial medulla Vertebrates: nervous system and sense organs |
| title | Inhibition of cardiac baroreflex by noxious thermal stimuli: A key role for lateral paragigantocellular serotonergic cells |
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