Silica-coated gold nanorods biofunctionalization for localized surface plasmon resonance (LSPR) biosensing
We introduce here the engineering of nanobiosensors designed from gold nanorods coated with an ultrathin layer of silica (AuNR@SiO2) and biofunctionalized with antibodies for the Localized Surface Plasmon Resonance (LSPR) biosensing of proteins. Despite the outstanding properties of AuNRs, their use...
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description | We introduce here the engineering of nanobiosensors designed from gold nanorods coated with an ultrathin layer of silica (AuNR@SiO2) and biofunctionalized with antibodies for the Localized Surface Plasmon Resonance (LSPR) biosensing of proteins. Despite the outstanding properties of AuNRs, their use for LSPR biosensing is limited due to the presence of the surfactant cetyltrimethylammonium bromide (CTAB) – mandatory for their synthesis - which forms a strongly-bounded and positively-charged bilayer at their surface and significantly complicates their bio-functionalization. When coated with a thin layer of silica, these nanomaterials exhibit an improved sensitivity to refractive index change which augurs for better analytical performances. Here, we undertook an in-depth investigation of the biofunctionalization of AuNR@SiO2via three different routes to design and test a label-free LSPR biosensor operating in solution. In the first route, we took advantage of the negatively charged external silica shell to immobilize anti-rabbit IgG antibody by electrostatic physisorption. In the second and third routes, the silica surface was reacted with thiol or aldehyde terminated silanes, subsequently utilized to covalently attach anti-rabbit IgG antibody to the surface. The resulting nanoprobes were characterized by a wide range of physical methods (TEM, XPS, DLS, ELS and UV-Visible spectroscopy) then tested for the biosensing of rabbit-IgG. The three nanobiosensors maintain an excellent colloidal stability after analyte recognition and exhibit extremely high analytical performances in terms of specificity and dynamic range, with an LoD down to 12 ng/mL.
[Display omitted]
•Three routes for surface functionalization of silica-coated gold nanorods were designed and characterized.•Nanobioconjugates were engineered by attaching antibodies to core-shell nanorods.•The nanobiosensors enabled sensitive, specific, and label-free detection of a protein analyte.•An LoD of 12 ng/mL was reached using benchtop UV-Visible spectrometer for readout.•Biofunctionalization route strongly impacted the analytical performances. |
doi_str_mv | 10.1016/j.talanta.2022.124245 |
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[Display omitted]
•Three routes for surface functionalization of silica-coated gold nanorods were designed and characterized.•Nanobioconjugates were engineered by attaching antibodies to core-shell nanorods.•The nanobiosensors enabled sensitive, specific, and label-free detection of a protein analyte.•An LoD of 12 ng/mL was reached using benchtop UV-Visible spectrometer for readout.•Biofunctionalization route strongly impacted the analytical performances.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2022.124245</identifier><identifier>PMID: 36610258</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Biosensing Techniques ; Chemical Sciences ; Core−Shell ; Gold - chemistry ; Gold nanorods ; Immunoglobulin G ; Immunosensing ; LSPR ; Nanotubes - chemistry ; Silica coating ; Silicon Dioxide - chemistry ; Surface chemistry ; Surface Plasmon Resonance - methods</subject><ispartof>Talanta (Oxford), 2023-04, Vol.255, p.124245-124245, Article 124245</ispartof><rights>2023 Elsevier B.V.</rights><rights>Copyright © 2023 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-9f8094b497a7bd68b1cd40cbec94902e4337a90f0ccd8d80d8d313105275f3723</citedby><cites>FETCH-LOGICAL-c376t-9f8094b497a7bd68b1cd40cbec94902e4337a90f0ccd8d80d8d313105275f3723</cites><orcidid>0000-0002-5500-0951 ; 0000-0003-3296-8430 ; 0000-0002-8006-1123 ; 0000-0003-3039-5659 ; 0000-0003-1935-4207</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2022.124245$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36610258$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03937215$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pellas, Vincent</creatorcontrib><creatorcontrib>Sallem, Fadoua</creatorcontrib><creatorcontrib>Blanchard, Juliette</creatorcontrib><creatorcontrib>Miche, Antoine</creatorcontrib><creatorcontrib>Concheso, Sara Martinez</creatorcontrib><creatorcontrib>Méthivier, Christophe</creatorcontrib><creatorcontrib>Salmain, Michèle</creatorcontrib><creatorcontrib>Boujday, Souhir</creatorcontrib><title>Silica-coated gold nanorods biofunctionalization for localized surface plasmon resonance (LSPR) biosensing</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>We introduce here the engineering of nanobiosensors designed from gold nanorods coated with an ultrathin layer of silica (AuNR@SiO2) and biofunctionalized with antibodies for the Localized Surface Plasmon Resonance (LSPR) biosensing of proteins. Despite the outstanding properties of AuNRs, their use for LSPR biosensing is limited due to the presence of the surfactant cetyltrimethylammonium bromide (CTAB) – mandatory for their synthesis - which forms a strongly-bounded and positively-charged bilayer at their surface and significantly complicates their bio-functionalization. When coated with a thin layer of silica, these nanomaterials exhibit an improved sensitivity to refractive index change which augurs for better analytical performances. Here, we undertook an in-depth investigation of the biofunctionalization of AuNR@SiO2via three different routes to design and test a label-free LSPR biosensor operating in solution. In the first route, we took advantage of the negatively charged external silica shell to immobilize anti-rabbit IgG antibody by electrostatic physisorption. In the second and third routes, the silica surface was reacted with thiol or aldehyde terminated silanes, subsequently utilized to covalently attach anti-rabbit IgG antibody to the surface. The resulting nanoprobes were characterized by a wide range of physical methods (TEM, XPS, DLS, ELS and UV-Visible spectroscopy) then tested for the biosensing of rabbit-IgG. The three nanobiosensors maintain an excellent colloidal stability after analyte recognition and exhibit extremely high analytical performances in terms of specificity and dynamic range, with an LoD down to 12 ng/mL.
[Display omitted]
•Three routes for surface functionalization of silica-coated gold nanorods were designed and characterized.•Nanobioconjugates were engineered by attaching antibodies to core-shell nanorods.•The nanobiosensors enabled sensitive, specific, and label-free detection of a protein analyte.•An LoD of 12 ng/mL was reached using benchtop UV-Visible spectrometer for readout.•Biofunctionalization route strongly impacted the analytical performances.</description><subject>Biosensing Techniques</subject><subject>Chemical Sciences</subject><subject>Core−Shell</subject><subject>Gold - chemistry</subject><subject>Gold nanorods</subject><subject>Immunoglobulin G</subject><subject>Immunosensing</subject><subject>LSPR</subject><subject>Nanotubes - chemistry</subject><subject>Silica coating</subject><subject>Silicon Dioxide - chemistry</subject><subject>Surface chemistry</subject><subject>Surface Plasmon Resonance - methods</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9r2zAUxUXpaLJ2H2HFj-mDs6s_tqynUsq2FgIba_csZEnOFBQrlexC9-kn4zSve5HE1e-cC-cg9BnDGgOuv-zWg_KqH9SaACFrTBhh1Rla4obTklacnqMlABWlwAwW6GNKOwAgFOgFWtC6xkCqZol2T847rUod1GBNsQ3eFL3qQwwmFa0L3djrwYVeefdXTY-iC7HwQU-DLEhj7JS2xcGrtM-_0aYM93my2jz9_HUzeSTbJ9dvr9CHTvlkPx3vS_T729fn-4dy8-P74_3dptSU10MpugYEa5ngiremblqsDQPdWi2YAGIZpVwJ6EBr05gG8kExxVARXnWUE3qJbmbfP8rLQ3R7Fd9kUE4-3G3kNMupZA5Xrzizq5k9xPAy2jTIvUva-pysDWOShNdYNCwLMlrNqI4hpWi7kzcGOVUid_JYiZwqkXMlWXd9XDG2e2tOqvcOMnA7AzaH8upslEk7myM0Llo9SBPcf1b8AyOVn34</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Pellas, Vincent</creator><creator>Sallem, Fadoua</creator><creator>Blanchard, Juliette</creator><creator>Miche, Antoine</creator><creator>Concheso, Sara Martinez</creator><creator>Méthivier, Christophe</creator><creator>Salmain, Michèle</creator><creator>Boujday, Souhir</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-5500-0951</orcidid><orcidid>https://orcid.org/0000-0003-3296-8430</orcidid><orcidid>https://orcid.org/0000-0002-8006-1123</orcidid><orcidid>https://orcid.org/0000-0003-3039-5659</orcidid><orcidid>https://orcid.org/0000-0003-1935-4207</orcidid></search><sort><creationdate>20230401</creationdate><title>Silica-coated gold nanorods biofunctionalization for localized surface plasmon resonance (LSPR) biosensing</title><author>Pellas, Vincent ; 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Despite the outstanding properties of AuNRs, their use for LSPR biosensing is limited due to the presence of the surfactant cetyltrimethylammonium bromide (CTAB) – mandatory for their synthesis - which forms a strongly-bounded and positively-charged bilayer at their surface and significantly complicates their bio-functionalization. When coated with a thin layer of silica, these nanomaterials exhibit an improved sensitivity to refractive index change which augurs for better analytical performances. Here, we undertook an in-depth investigation of the biofunctionalization of AuNR@SiO2via three different routes to design and test a label-free LSPR biosensor operating in solution. In the first route, we took advantage of the negatively charged external silica shell to immobilize anti-rabbit IgG antibody by electrostatic physisorption. In the second and third routes, the silica surface was reacted with thiol or aldehyde terminated silanes, subsequently utilized to covalently attach anti-rabbit IgG antibody to the surface. The resulting nanoprobes were characterized by a wide range of physical methods (TEM, XPS, DLS, ELS and UV-Visible spectroscopy) then tested for the biosensing of rabbit-IgG. The three nanobiosensors maintain an excellent colloidal stability after analyte recognition and exhibit extremely high analytical performances in terms of specificity and dynamic range, with an LoD down to 12 ng/mL.
[Display omitted]
•Three routes for surface functionalization of silica-coated gold nanorods were designed and characterized.•Nanobioconjugates were engineered by attaching antibodies to core-shell nanorods.•The nanobiosensors enabled sensitive, specific, and label-free detection of a protein analyte.•An LoD of 12 ng/mL was reached using benchtop UV-Visible spectrometer for readout.•Biofunctionalization route strongly impacted the analytical performances.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>36610258</pmid><doi>10.1016/j.talanta.2022.124245</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-5500-0951</orcidid><orcidid>https://orcid.org/0000-0003-3296-8430</orcidid><orcidid>https://orcid.org/0000-0002-8006-1123</orcidid><orcidid>https://orcid.org/0000-0003-3039-5659</orcidid><orcidid>https://orcid.org/0000-0003-1935-4207</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Biosensing Techniques Chemical Sciences Core−Shell Gold - chemistry Gold nanorods Immunoglobulin G Immunosensing LSPR Nanotubes - chemistry Silica coating Silicon Dioxide - chemistry Surface chemistry Surface Plasmon Resonance - methods |
title | Silica-coated gold nanorods biofunctionalization for localized surface plasmon resonance (LSPR) biosensing |
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