Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France
The occurrence of the hemolysin genes, tdh and trh, in Vibrio parahaemolyticus strains isolated from environmental samples collected in two French coastal areas, clinical samples, and seafood products imported into France was studied. Polymerase chain reaction (PCR) with two sets of primers was used...
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| Veröffentlicht in: | International journal of food microbiology 2004-03, Vol.91 (3), p.319-325 |
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| container_title | International journal of food microbiology |
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| creator | Robert-Pillot, Annick Guénolé, Alain Lesne, Jean Delesmont, Régis Fournier, Jean-Michel Quilici, Marie-Laure |
| description | The occurrence of the hemolysin genes,
tdh and
trh, in
Vibrio parahaemolyticus strains isolated from environmental samples collected in two French coastal areas, clinical samples, and seafood products imported into France was studied. Polymerase chain reaction (PCR) with two sets of primers was used to detect the hemolysin genes. Most of the clinical isolates (91%) and 1.5% of the isolates from seafood possessed the hemolysin genes. Three and fifteen percent, respectively, of the two groups of environmental strains carried the hemolysin genes depending on the geographic site. The
tdh and
trh genes play important roles in virulence. Thus, our results indicate that pathogenic
V. parahaemolyticus isolates are present in French coastal areas and in seafood imported into France. Furthermore, they may also be present in French seafood products. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2003.07.006 |
| format | Article |
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tdh and
trh, in
Vibrio parahaemolyticus strains isolated from environmental samples collected in two French coastal areas, clinical samples, and seafood products imported into France was studied. Polymerase chain reaction (PCR) with two sets of primers was used to detect the hemolysin genes. Most of the clinical isolates (91%) and 1.5% of the isolates from seafood possessed the hemolysin genes. Three and fifteen percent, respectively, of the two groups of environmental strains carried the hemolysin genes depending on the geographic site. The
tdh and
trh genes play important roles in virulence. Thus, our results indicate that pathogenic
V. parahaemolyticus isolates are present in French coastal areas and in seafood imported into France. Furthermore, they may also be present in French seafood products.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2003.07.006</identifier><identifier>PMID: 14984780</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; bacterial contamination ; Biological and medical sciences ; coastal water ; Environmental Microbiology ; feces ; Fish and seafood industries ; food contamination ; Food Contamination - analysis ; Food industries ; Food Microbiology ; food pathogens ; France ; French coastal areas ; Fundamental and applied biological sciences. Psychology ; genes ; hemolysin genes ; Hemolysin Proteins - biosynthesis ; Hemolysin Proteins - genetics ; hemolysins ; humans ; imports ; isolation ; Life Sciences ; Marine ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Santé publique et épidémiologie ; screening ; Seafood - microbiology ; seawater ; Shellfish ; Shellfish - microbiology ; tdh and trh genes ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - genetics ; Vibrio parahaemolyticus - isolation & purification ; Vibrio parahaemolyticus - pathogenicity ; virulence ; Virulence - genetics ; virulence genes ; Water</subject><ispartof>International journal of food microbiology, 2004-03, Vol.91 (3), p.319-325</ispartof><rights>2002 Elsevier B.V.</rights><rights>2004 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-764fb11fc347210a85985a3bee8d648f43e1ea6989eb1be3c3c4c9c9ec319f713</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2003.07.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15483948$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14984780$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://ehesp.hal.science/hal-03866336$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Robert-Pillot, Annick</creatorcontrib><creatorcontrib>Guénolé, Alain</creatorcontrib><creatorcontrib>Lesne, Jean</creatorcontrib><creatorcontrib>Delesmont, Régis</creatorcontrib><creatorcontrib>Fournier, Jean-Michel</creatorcontrib><creatorcontrib>Quilici, Marie-Laure</creatorcontrib><title>Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>The occurrence of the hemolysin genes,
tdh and
trh, in
Vibrio parahaemolyticus strains isolated from environmental samples collected in two French coastal areas, clinical samples, and seafood products imported into France was studied. Polymerase chain reaction (PCR) with two sets of primers was used to detect the hemolysin genes. Most of the clinical isolates (91%) and 1.5% of the isolates from seafood possessed the hemolysin genes. Three and fifteen percent, respectively, of the two groups of environmental strains carried the hemolysin genes depending on the geographic site. The
tdh and
trh genes play important roles in virulence. Thus, our results indicate that pathogenic
V. parahaemolyticus isolates are present in French coastal areas and in seafood imported into France. Furthermore, they may also be present in French seafood products.</description><subject>Animals</subject><subject>bacterial contamination</subject><subject>Biological and medical sciences</subject><subject>coastal water</subject><subject>Environmental Microbiology</subject><subject>feces</subject><subject>Fish and seafood industries</subject><subject>food contamination</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>France</subject><subject>French coastal areas</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>hemolysin genes</subject><subject>Hemolysin Proteins - biosynthesis</subject><subject>Hemolysin Proteins - genetics</subject><subject>hemolysins</subject><subject>humans</subject><subject>imports</subject><subject>isolation</subject><subject>Life Sciences</subject><subject>Marine</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Santé publique et épidémiologie</subject><subject>screening</subject><subject>Seafood - microbiology</subject><subject>seawater</subject><subject>Shellfish</subject><subject>Shellfish - microbiology</subject><subject>tdh and trh genes</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - genetics</subject><subject>Vibrio parahaemolyticus - isolation & purification</subject><subject>Vibrio parahaemolyticus - pathogenicity</subject><subject>virulence</subject><subject>Virulence - genetics</subject><subject>virulence genes</subject><subject>Water</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkstuEzEUhi0EoiHwCmAWILHIYMdzsZdVRClSpC6gbK0znuOOo5k42JNGfSzeELsTUZYsLFvH338u_k3Ie84Kznj9eVe4nfW-G50JvlgzJgrWFIzVz8iCy0atRFmz52SRWLniNasuyKsYd4yxSgj2klzwUsmykWxBft8YcwwB9wapt3TqkU5dT2Hf0Sn09A73GKnb05-uDc7TAwToAUc_PEzOHNNV9ANMibHBj_SUjiE-qgOcaOxxGKyLPTV-GNBM2OVc08nTq1wyxyFOMFAICLPuMU9EyPNRNx58mFVT1kBq8zV5YWGI-Oa8L8nt1Zcfm-vV9ubrt83ldmVKtZ5WTV3alnNrRNmsOQNZKVmBaBFlV5fSlgI5Qq2kwpa3KIwwpVFGoRFc2YaLJfk05-1h0IfgRggP2oPT15dbnWNMyLoWor7P7MeZPQT_64hx0qOLJg0Pe_THqLlkdcWbKoFqBpNxMQa0fzNzprO3eqf_8VZnbzVrdPI2ad-eixzbEbsn5dnMBHw4AxANDDY_l4tPXFVKodJaknczZ8FruAuJuf2-ZlwwpoRoxDoRm5nA9MD3DoOOxuU_0rmQbNSdd__R8B9SzNTL</recordid><startdate>20040315</startdate><enddate>20040315</enddate><creator>Robert-Pillot, Annick</creator><creator>Guénolé, Alain</creator><creator>Lesne, Jean</creator><creator>Delesmont, Régis</creator><creator>Fournier, Jean-Michel</creator><creator>Quilici, Marie-Laure</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>1XC</scope></search><sort><creationdate>20040315</creationdate><title>Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France</title><author>Robert-Pillot, Annick ; Guénolé, Alain ; Lesne, Jean ; Delesmont, Régis ; Fournier, Jean-Michel ; Quilici, Marie-Laure</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-764fb11fc347210a85985a3bee8d648f43e1ea6989eb1be3c3c4c9c9ec319f713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>bacterial contamination</topic><topic>Biological and medical sciences</topic><topic>coastal water</topic><topic>Environmental Microbiology</topic><topic>feces</topic><topic>Fish and seafood industries</topic><topic>food contamination</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>France</topic><topic>French coastal areas</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>hemolysin genes</topic><topic>Hemolysin Proteins - biosynthesis</topic><topic>Hemolysin Proteins - genetics</topic><topic>hemolysins</topic><topic>humans</topic><topic>imports</topic><topic>isolation</topic><topic>Life Sciences</topic><topic>Marine</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Santé publique et épidémiologie</topic><topic>screening</topic><topic>Seafood - microbiology</topic><topic>seawater</topic><topic>Shellfish</topic><topic>Shellfish - microbiology</topic><topic>tdh and trh genes</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio parahaemolyticus - genetics</topic><topic>Vibrio parahaemolyticus - isolation & purification</topic><topic>Vibrio parahaemolyticus - pathogenicity</topic><topic>virulence</topic><topic>Virulence - genetics</topic><topic>virulence genes</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robert-Pillot, Annick</creatorcontrib><creatorcontrib>Guénolé, Alain</creatorcontrib><creatorcontrib>Lesne, Jean</creatorcontrib><creatorcontrib>Delesmont, Régis</creatorcontrib><creatorcontrib>Fournier, Jean-Michel</creatorcontrib><creatorcontrib>Quilici, Marie-Laure</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robert-Pillot, Annick</au><au>Guénolé, Alain</au><au>Lesne, Jean</au><au>Delesmont, Régis</au><au>Fournier, Jean-Michel</au><au>Quilici, Marie-Laure</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2004-03-15</date><risdate>2004</risdate><volume>91</volume><issue>3</issue><spage>319</spage><epage>325</epage><pages>319-325</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>The occurrence of the hemolysin genes,
tdh and
trh, in
Vibrio parahaemolyticus strains isolated from environmental samples collected in two French coastal areas, clinical samples, and seafood products imported into France was studied. Polymerase chain reaction (PCR) with two sets of primers was used to detect the hemolysin genes. Most of the clinical isolates (91%) and 1.5% of the isolates from seafood possessed the hemolysin genes. Three and fifteen percent, respectively, of the two groups of environmental strains carried the hemolysin genes depending on the geographic site. The
tdh and
trh genes play important roles in virulence. Thus, our results indicate that pathogenic
V. parahaemolyticus isolates are present in French coastal areas and in seafood imported into France. Furthermore, they may also be present in French seafood products.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>14984780</pmid><doi>10.1016/j.ijfoodmicro.2003.07.006</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-1605 |
| ispartof | International journal of food microbiology, 2004-03, Vol.91 (3), p.319-325 |
| issn | 0168-1605 1879-3460 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03866336v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals bacterial contamination Biological and medical sciences coastal water Environmental Microbiology feces Fish and seafood industries food contamination Food Contamination - analysis Food industries Food Microbiology food pathogens France French coastal areas Fundamental and applied biological sciences. Psychology genes hemolysin genes Hemolysin Proteins - biosynthesis Hemolysin Proteins - genetics hemolysins humans imports isolation Life Sciences Marine polymerase chain reaction Polymerase Chain Reaction - methods Santé publique et épidémiologie screening Seafood - microbiology seawater Shellfish Shellfish - microbiology tdh and trh genes Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - isolation & purification Vibrio parahaemolyticus - pathogenicity virulence Virulence - genetics virulence genes Water |
| title | Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France |
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