Analysis and purification of ssRNA and dsRNA molecules using asymmetrical flow field flow fractionation

•AF4 facilitated purification of long ssRNA and dsRNA molecules in native conditions.•AF4 preserved native RNA conformation and enabled detection of RNA multimers.•New information on solution properties of RNA was obtained.•Use of semipreparative AF4 channel allowed milligram RNA loads.•AF4 purified...

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