In situ production of innate immune cells in murine white adipose tissue
White adipose tissue (WAT) is the focus of new interest because of the presence of an abundant and complex immune cell population that is involved in key pathologies such as metabolic syndrome. Based on in vivo reconstitution assays, it is thought that these immune cells are derived from the bone ma...
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| Veröffentlicht in: | Blood 2012-12, Vol.120 (25), p.4952-4962 |
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| creator | Poglio, Sandrine De Toni, Fabienne Lewandowski, Daniel Minot, Adeline Arnaud, Emmanuelle Barroca, Vilma Laharrague, Patrick Casteilla, Louis Cousin, Béatrice |
| description | White adipose tissue (WAT) is the focus of new interest because of the presence of an abundant and complex immune cell population that is involved in key pathologies such as metabolic syndrome. Based on in vivo reconstitution assays, it is thought that these immune cells are derived from the bone marrow (BM). However, previous studies have shown that WAT exhibits specific hematopoietic activity exerted by an unknown subpopulation of cells. In the present study, we prospectively isolated a peculiar hematopoietic stem/progenitor cell population from murine WAT. The cells are phenotypically similar to BM hematopoietic stem cells and are able to differentiate into both myeloid and lymphoid lineages in vitro. In competitive repopulation assays in vivo, they reconstituted the innate immune compartment in WAT preferentially and more efficiently than BM cells, but did not reconstitute hematopoietic organs. They were also able to give rise to multilineage engraftment in both secondary recipients and in utero transplantation. Therefore, we propose that WAT hematopoietic cells constitute a population of immature cells that are able to renew innate immune cell populations. Considering the amount of WAT in adults, our results suggest that WAT hematopoietic activity controls WAT inflammatory processes and also supports innate immune responses in other organs. |
| doi_str_mv | 10.1182/blood-2012-01-406959 |
| format | Article |
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Based on in vivo reconstitution assays, it is thought that these immune cells are derived from the bone marrow (BM). However, previous studies have shown that WAT exhibits specific hematopoietic activity exerted by an unknown subpopulation of cells. In the present study, we prospectively isolated a peculiar hematopoietic stem/progenitor cell population from murine WAT. The cells are phenotypically similar to BM hematopoietic stem cells and are able to differentiate into both myeloid and lymphoid lineages in vitro. In competitive repopulation assays in vivo, they reconstituted the innate immune compartment in WAT preferentially and more efficiently than BM cells, but did not reconstitute hematopoietic organs. They were also able to give rise to multilineage engraftment in both secondary recipients and in utero transplantation. Therefore, we propose that WAT hematopoietic cells constitute a population of immature cells that are able to renew innate immune cell populations. Considering the amount of WAT in adults, our results suggest that WAT hematopoietic activity controls WAT inflammatory processes and also supports innate immune responses in other organs.</description><identifier>ISSN: 0006-4971</identifier><identifier>ISSN: 1528-0020</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood-2012-01-406959</identifier><identifier>PMID: 23071275</identifier><language>eng</language><publisher>Washington, DC: Elsevier Inc</publisher><subject>Adipose Tissue, White - cytology ; Adipose Tissue, White - immunology ; Adipose Tissue, White - transplantation ; Animals ; Antigens, Ly - analysis ; Biological and medical sciences ; Cell Differentiation ; Female ; Hematologic and hematopoietic diseases ; Hematopoietic Stem Cells - cytology ; Hematopoietic Stem Cells - immunology ; Immunity, Innate ; Killer Cells, Natural - cytology ; Killer Cells, Natural - immunology ; Life Sciences ; Lymphocytes - cytology ; Lymphocytes - immunology ; Male ; Medical sciences ; Membrane Proteins - analysis ; Mice ; Mice, Inbred C57BL ; Myeloid Cells - cytology ; Myeloid Cells - immunology ; Proto-Oncogene Proteins c-kit - analysis</subject><ispartof>Blood, 2012-12, Vol.120 (25), p.4952-4962</ispartof><rights>2012 American Society of Hematology</rights><rights>2014 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-4b615abeddd649dae0630b9c5cf4cd94b6e69766ef1cace9be73c22e02725dcc3</citedby><cites>FETCH-LOGICAL-c505t-4b615abeddd649dae0630b9c5cf4cd94b6e69766ef1cace9be73c22e02725dcc3</cites><orcidid>0000-0003-2952-4601 ; 0000-0001-9647-3248</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26727021$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23071275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03737786$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Poglio, Sandrine</creatorcontrib><creatorcontrib>De Toni, Fabienne</creatorcontrib><creatorcontrib>Lewandowski, Daniel</creatorcontrib><creatorcontrib>Minot, Adeline</creatorcontrib><creatorcontrib>Arnaud, Emmanuelle</creatorcontrib><creatorcontrib>Barroca, Vilma</creatorcontrib><creatorcontrib>Laharrague, Patrick</creatorcontrib><creatorcontrib>Casteilla, Louis</creatorcontrib><creatorcontrib>Cousin, Béatrice</creatorcontrib><title>In situ production of innate immune cells in murine white adipose tissue</title><title>Blood</title><addtitle>Blood</addtitle><description>White adipose tissue (WAT) is the focus of new interest because of the presence of an abundant and complex immune cell population that is involved in key pathologies such as metabolic syndrome. 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Considering the amount of WAT in adults, our results suggest that WAT hematopoietic activity controls WAT inflammatory processes and also supports innate immune responses in other organs.</description><subject>Adipose Tissue, White - cytology</subject><subject>Adipose Tissue, White - immunology</subject><subject>Adipose Tissue, White - transplantation</subject><subject>Animals</subject><subject>Antigens, Ly - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Female</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Immunity, Innate</subject><subject>Killer Cells, Natural - cytology</subject><subject>Killer Cells, Natural - immunology</subject><subject>Life Sciences</subject><subject>Lymphocytes - cytology</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Proteins - analysis</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Myeloid Cells - cytology</subject><subject>Myeloid Cells - immunology</subject><subject>Proto-Oncogene Proteins c-kit - analysis</subject><issn>0006-4971</issn><issn>1528-0020</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFO3DAQhq2qqGxp36BCuVRqD2nHdmyvL0gIQRdpJS70bDn2RBgl8WInVLx9vWQLN3qyZuYbzz_zE_KFwg9K1-xn28foawaU1UDrBqQW-h1ZUcHWNQCD92QFALJutKLH5GPO9wC04Ux8IMeMg6JMiRXZXI9VDtNc7VL0s5tCHKvYVWEc7YRVGIZ5xMph3-eSq4Y5hRL_uQulaH3YxYzVFHKe8RM56myf8fPhPSG_ry5vLzb19ubX9cX5tnYCxFQ3raTCtui9l432FkFyaLUTrmuc16WMUispsaPOOtQtKu4YQ2CKCe8cPyHfl3_vbG92KQw2PZlog9mcb80-B1xxpdbykRb228KW5R5mzJMZQt4vY0eMczZUCCq5gjX_P8oaYJRxoQvaLKhLMeeE3YsMCmZvjXm2xuytMUDNYk1pOz1MmNsB_UvTPy8K8PUA2Oxs3yU7upBfOamYKhoKd7ZwWM78GDCZ7AKODn1I6CbjY3hbyV80gav6</recordid><startdate>20121213</startdate><enddate>20121213</enddate><creator>Poglio, Sandrine</creator><creator>De Toni, Fabienne</creator><creator>Lewandowski, Daniel</creator><creator>Minot, Adeline</creator><creator>Arnaud, Emmanuelle</creator><creator>Barroca, Vilma</creator><creator>Laharrague, Patrick</creator><creator>Casteilla, Louis</creator><creator>Cousin, Béatrice</creator><general>Elsevier Inc</general><general>Americain Society of Hematology</general><general>American Society of Hematology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2952-4601</orcidid><orcidid>https://orcid.org/0000-0001-9647-3248</orcidid></search><sort><creationdate>20121213</creationdate><title>In situ production of innate immune cells in murine white adipose tissue</title><author>Poglio, Sandrine ; De Toni, Fabienne ; Lewandowski, Daniel ; Minot, Adeline ; Arnaud, Emmanuelle ; Barroca, Vilma ; Laharrague, Patrick ; Casteilla, Louis ; Cousin, Béatrice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-4b615abeddd649dae0630b9c5cf4cd94b6e69766ef1cace9be73c22e02725dcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adipose Tissue, White - cytology</topic><topic>Adipose Tissue, White - immunology</topic><topic>Adipose Tissue, White - transplantation</topic><topic>Animals</topic><topic>Antigens, Ly - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Female</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Hematopoietic Stem Cells - immunology</topic><topic>Immunity, Innate</topic><topic>Killer Cells, Natural - cytology</topic><topic>Killer Cells, Natural - immunology</topic><topic>Life Sciences</topic><topic>Lymphocytes - cytology</topic><topic>Lymphocytes - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Proteins - analysis</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Myeloid Cells - cytology</topic><topic>Myeloid Cells - immunology</topic><topic>Proto-Oncogene Proteins c-kit - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poglio, Sandrine</creatorcontrib><creatorcontrib>De Toni, Fabienne</creatorcontrib><creatorcontrib>Lewandowski, Daniel</creatorcontrib><creatorcontrib>Minot, Adeline</creatorcontrib><creatorcontrib>Arnaud, Emmanuelle</creatorcontrib><creatorcontrib>Barroca, Vilma</creatorcontrib><creatorcontrib>Laharrague, Patrick</creatorcontrib><creatorcontrib>Casteilla, Louis</creatorcontrib><creatorcontrib>Cousin, Béatrice</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poglio, Sandrine</au><au>De Toni, Fabienne</au><au>Lewandowski, Daniel</au><au>Minot, Adeline</au><au>Arnaud, Emmanuelle</au><au>Barroca, Vilma</au><au>Laharrague, Patrick</au><au>Casteilla, Louis</au><au>Cousin, Béatrice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ production of innate immune cells in murine white adipose tissue</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>2012-12-13</date><risdate>2012</risdate><volume>120</volume><issue>25</issue><spage>4952</spage><epage>4962</epage><pages>4952-4962</pages><issn>0006-4971</issn><issn>1528-0020</issn><eissn>1528-0020</eissn><abstract>White adipose tissue (WAT) is the focus of new interest because of the presence of an abundant and complex immune cell population that is involved in key pathologies such as metabolic syndrome. Based on in vivo reconstitution assays, it is thought that these immune cells are derived from the bone marrow (BM). However, previous studies have shown that WAT exhibits specific hematopoietic activity exerted by an unknown subpopulation of cells. In the present study, we prospectively isolated a peculiar hematopoietic stem/progenitor cell population from murine WAT. The cells are phenotypically similar to BM hematopoietic stem cells and are able to differentiate into both myeloid and lymphoid lineages in vitro. In competitive repopulation assays in vivo, they reconstituted the innate immune compartment in WAT preferentially and more efficiently than BM cells, but did not reconstitute hematopoietic organs. They were also able to give rise to multilineage engraftment in both secondary recipients and in utero transplantation. Therefore, we propose that WAT hematopoietic cells constitute a population of immature cells that are able to renew innate immune cell populations. Considering the amount of WAT in adults, our results suggest that WAT hematopoietic activity controls WAT inflammatory processes and also supports innate immune responses in other organs.</abstract><cop>Washington, DC</cop><pub>Elsevier Inc</pub><pmid>23071275</pmid><doi>10.1182/blood-2012-01-406959</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-2952-4601</orcidid><orcidid>https://orcid.org/0000-0001-9647-3248</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipose Tissue, White - cytology Adipose Tissue, White - immunology Adipose Tissue, White - transplantation Animals Antigens, Ly - analysis Biological and medical sciences Cell Differentiation Female Hematologic and hematopoietic diseases Hematopoietic Stem Cells - cytology Hematopoietic Stem Cells - immunology Immunity, Innate Killer Cells, Natural - cytology Killer Cells, Natural - immunology Life Sciences Lymphocytes - cytology Lymphocytes - immunology Male Medical sciences Membrane Proteins - analysis Mice Mice, Inbred C57BL Myeloid Cells - cytology Myeloid Cells - immunology Proto-Oncogene Proteins c-kit - analysis |
| title | In situ production of innate immune cells in murine white adipose tissue |
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