The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23
Nitrate and potassium nutrition is tightly coordinated in vascular plants. Physiological and molecular genetics studies have demonstrated that several NPF/NRT1 nitrate transporters have a significant impact on both uptake and the root-shoot partition of these nutrients. However, how these traits are...
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| Veröffentlicht in: | Plant physiology and biochemistry 2021-11, Vol.168, p.239-251 |
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| creator | Morales de los Ríos, Laura Corratgé-Faillie, Claire Raddatz, Natalia Mendoza, Imelda Lindahl, Marika de Angeli, Alexis Lacombe, Benoit Quintero, Francisco J. Pardo, José M. |
| description | Nitrate and potassium nutrition is tightly coordinated in vascular plants. Physiological and molecular genetics studies have demonstrated that several NPF/NRT1 nitrate transporters have a significant impact on both uptake and the root-shoot partition of these nutrients. However, how these traits are biochemically connected remain controversial since some NPF proteins, e.g. NPF7.3/NRT1.5, have been suggested to mediate K+/H+ exchange instead of nitrate fluxes. Here we show that NPF6.2/NRT1.4, a protein that gates nitrate accumulation at the leaf petiole of Arabidopsis thaliana, also affects the root/shoot distribution of potassium. We demonstrate that NPF6.2/NRT1.4 is a plasma membrane nitrate transporter phosphorylated at threonine-98 by the CIPK23 protein kinase that is a regulatory hub for nitrogen and potassium nutrition. Heterologous expression of NPF6.2/NRT1.4 and NPF7.3/NRT1.5 in yeast mutants with altered potassium uptake and efflux systems showed no evidence of nitrate-dependent potassium transport by these proteins.
•Nitrate and potassium nutrition is tightly coordinated in vascular plants.•Protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter.•Mutant plants lacking NPF6.2/NRT1.4 have disturbed potassium contents.•NPF6.2/NRT1.4 is a target of protein kinase CIPK23. |
| doi_str_mv | 10.1016/j.plaphy.2021.10.016 |
| format | Article |
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•Nitrate and potassium nutrition is tightly coordinated in vascular plants.•Protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter.•Mutant plants lacking NPF6.2/NRT1.4 have disturbed potassium contents.•NPF6.2/NRT1.4 is a target of protein kinase CIPK23.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>EISSN: 0981-9428</identifier><identifier>DOI: 10.1016/j.plaphy.2021.10.016</identifier><identifier>PMID: 34656860</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Anion Transport Proteins ; Anion Transport Proteins - genetics ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Cell Membrane ; Cell Membrane - metabolism ; Ion transport ; Life Sciences ; Mutation ; Nitrate ; Nitrate Transporters ; Nitrates ; Nitrates - metabolism ; Plant nutrition ; Plant Proteins ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Roots ; Plant Roots - metabolism ; Plant Shoots ; Plant Shoots - metabolism ; Potassium ; Protein Kinases ; Vegetal Biology</subject><ispartof>Plant physiology and biochemistry, 2021-11, Vol.168, p.239-251</ispartof><rights>2021 Consejo Superior de Investigaciones Cientificas</rights><rights>Copyright © 2021 Consejo Superior de Investigaciones Cientificas. Published by Elsevier Masson SAS.. All rights reserved.</rights><rights>Attribution - NonCommercial - NoDerivatives</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-6a2e15856b65826b1aa5481ffc965dc43b438f732e604bc6f0bed6447e9af43a3</citedby><cites>FETCH-LOGICAL-c442t-6a2e15856b65826b1aa5481ffc965dc43b438f732e604bc6f0bed6447e9af43a3</cites><orcidid>0000-0002-5738-6712 ; 0000-0002-1801-4131 ; 0000-0001-9924-3093 ; 0000-0003-4510-8624 ; 0000-0002-2673-3961 ; 0000-0003-3072-7932</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0981942821005350$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34656860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-03713227$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Morales de los Ríos, Laura</creatorcontrib><creatorcontrib>Corratgé-Faillie, Claire</creatorcontrib><creatorcontrib>Raddatz, Natalia</creatorcontrib><creatorcontrib>Mendoza, Imelda</creatorcontrib><creatorcontrib>Lindahl, Marika</creatorcontrib><creatorcontrib>de Angeli, Alexis</creatorcontrib><creatorcontrib>Lacombe, Benoit</creatorcontrib><creatorcontrib>Quintero, Francisco J.</creatorcontrib><creatorcontrib>Pardo, José M.</creatorcontrib><title>The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Nitrate and potassium nutrition is tightly coordinated in vascular plants. Physiological and molecular genetics studies have demonstrated that several NPF/NRT1 nitrate transporters have a significant impact on both uptake and the root-shoot partition of these nutrients. However, how these traits are biochemically connected remain controversial since some NPF proteins, e.g. NPF7.3/NRT1.5, have been suggested to mediate K+/H+ exchange instead of nitrate fluxes. Here we show that NPF6.2/NRT1.4, a protein that gates nitrate accumulation at the leaf petiole of Arabidopsis thaliana, also affects the root/shoot distribution of potassium. We demonstrate that NPF6.2/NRT1.4 is a plasma membrane nitrate transporter phosphorylated at threonine-98 by the CIPK23 protein kinase that is a regulatory hub for nitrogen and potassium nutrition. Heterologous expression of NPF6.2/NRT1.4 and NPF7.3/NRT1.5 in yeast mutants with altered potassium uptake and efflux systems showed no evidence of nitrate-dependent potassium transport by these proteins.
•Nitrate and potassium nutrition is tightly coordinated in vascular plants.•Protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter.•Mutant plants lacking NPF6.2/NRT1.4 have disturbed potassium contents.•NPF6.2/NRT1.4 is a target of protein kinase CIPK23.</description><subject>Anion Transport Proteins</subject><subject>Anion Transport Proteins - genetics</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Cell Membrane</subject><subject>Cell Membrane - metabolism</subject><subject>Ion transport</subject><subject>Life Sciences</subject><subject>Mutation</subject><subject>Nitrate</subject><subject>Nitrate Transporters</subject><subject>Nitrates</subject><subject>Nitrates - metabolism</subject><subject>Plant nutrition</subject><subject>Plant Proteins</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Roots</subject><subject>Plant Roots - metabolism</subject><subject>Plant Shoots</subject><subject>Plant Shoots - metabolism</subject><subject>Potassium</subject><subject>Protein Kinases</subject><subject>Vegetal Biology</subject><issn>0981-9428</issn><issn>1873-2690</issn><issn>0981-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhS0EotPCP0DIS1gk9SuOs0EajSitGJUKDWvrJrlhPOSF7anUf49HaWfJxpaOv3uO7UPIB85yzri-PuRzD_P-KRdM8CTlSXxFVtyUMhO6Yq_JilWGZ5US5oJchnBgjAlVyrfkQipdaKPZisTdHunaQ-3aaQ4u0NlPEd1I7x9udC6u73_ueK5oOgCa8sIAdMCh9jAiHV30EJGmdQzz5CN6CmObyAj-N0Y6dWe7P26EgHRz9_BdyHfkTQd9wPfP-xX5dfN1t7nNtj--3W3W26xRSsRMg0BemELXujBC1xygUIZ3XVPpom2UrJU0XSkFaqbqRnesxlYrVWIFnZIgr8jnxXcPvZ29G8A_2QmcvV1v7UljsuRSiPKRJ_bTwqYb_z1iiHZwocG-Ty-djsGKwkgpSq1YQtWCNn4KwWN39ubMnrqxB7t0Y0_dnNQkprGPzwnHesD2PPRSRgK-LACmP3l06G1oHI4Nts5jE207uf8n_AOXAZ-Z</recordid><startdate>202111</startdate><enddate>202111</enddate><creator>Morales de los Ríos, Laura</creator><creator>Corratgé-Faillie, Claire</creator><creator>Raddatz, Natalia</creator><creator>Mendoza, Imelda</creator><creator>Lindahl, Marika</creator><creator>de Angeli, Alexis</creator><creator>Lacombe, Benoit</creator><creator>Quintero, Francisco J.</creator><creator>Pardo, José M.</creator><general>Elsevier Masson SAS</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-5738-6712</orcidid><orcidid>https://orcid.org/0000-0002-1801-4131</orcidid><orcidid>https://orcid.org/0000-0001-9924-3093</orcidid><orcidid>https://orcid.org/0000-0003-4510-8624</orcidid><orcidid>https://orcid.org/0000-0002-2673-3961</orcidid><orcidid>https://orcid.org/0000-0003-3072-7932</orcidid></search><sort><creationdate>202111</creationdate><title>The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23</title><author>Morales de los Ríos, Laura ; Corratgé-Faillie, Claire ; Raddatz, Natalia ; Mendoza, Imelda ; Lindahl, Marika ; de Angeli, Alexis ; Lacombe, Benoit ; Quintero, Francisco J. ; Pardo, José M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-6a2e15856b65826b1aa5481ffc965dc43b438f732e604bc6f0bed6447e9af43a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Anion Transport Proteins</topic><topic>Anion Transport Proteins - genetics</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Cell Membrane</topic><topic>Cell Membrane - metabolism</topic><topic>Ion transport</topic><topic>Life Sciences</topic><topic>Mutation</topic><topic>Nitrate</topic><topic>Nitrate Transporters</topic><topic>Nitrates</topic><topic>Nitrates - metabolism</topic><topic>Plant nutrition</topic><topic>Plant Proteins</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Roots</topic><topic>Plant Roots - metabolism</topic><topic>Plant Shoots</topic><topic>Plant Shoots - metabolism</topic><topic>Potassium</topic><topic>Protein Kinases</topic><topic>Vegetal Biology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morales de los Ríos, Laura</creatorcontrib><creatorcontrib>Corratgé-Faillie, Claire</creatorcontrib><creatorcontrib>Raddatz, Natalia</creatorcontrib><creatorcontrib>Mendoza, Imelda</creatorcontrib><creatorcontrib>Lindahl, Marika</creatorcontrib><creatorcontrib>de Angeli, Alexis</creatorcontrib><creatorcontrib>Lacombe, Benoit</creatorcontrib><creatorcontrib>Quintero, Francisco J.</creatorcontrib><creatorcontrib>Pardo, José M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morales de los Ríos, Laura</au><au>Corratgé-Faillie, Claire</au><au>Raddatz, Natalia</au><au>Mendoza, Imelda</au><au>Lindahl, Marika</au><au>de Angeli, Alexis</au><au>Lacombe, Benoit</au><au>Quintero, Francisco J.</au><au>Pardo, José M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2021-11</date><risdate>2021</risdate><volume>168</volume><spage>239</spage><epage>251</epage><pages>239-251</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><eissn>0981-9428</eissn><abstract>Nitrate and potassium nutrition is tightly coordinated in vascular plants. Physiological and molecular genetics studies have demonstrated that several NPF/NRT1 nitrate transporters have a significant impact on both uptake and the root-shoot partition of these nutrients. However, how these traits are biochemically connected remain controversial since some NPF proteins, e.g. NPF7.3/NRT1.5, have been suggested to mediate K+/H+ exchange instead of nitrate fluxes. Here we show that NPF6.2/NRT1.4, a protein that gates nitrate accumulation at the leaf petiole of Arabidopsis thaliana, also affects the root/shoot distribution of potassium. We demonstrate that NPF6.2/NRT1.4 is a plasma membrane nitrate transporter phosphorylated at threonine-98 by the CIPK23 protein kinase that is a regulatory hub for nitrogen and potassium nutrition. Heterologous expression of NPF6.2/NRT1.4 and NPF7.3/NRT1.5 in yeast mutants with altered potassium uptake and efflux systems showed no evidence of nitrate-dependent potassium transport by these proteins.
•Nitrate and potassium nutrition is tightly coordinated in vascular plants.•Protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter.•Mutant plants lacking NPF6.2/NRT1.4 have disturbed potassium contents.•NPF6.2/NRT1.4 is a target of protein kinase CIPK23.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>34656860</pmid><doi>10.1016/j.plaphy.2021.10.016</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-5738-6712</orcidid><orcidid>https://orcid.org/0000-0002-1801-4131</orcidid><orcidid>https://orcid.org/0000-0001-9924-3093</orcidid><orcidid>https://orcid.org/0000-0003-4510-8624</orcidid><orcidid>https://orcid.org/0000-0002-2673-3961</orcidid><orcidid>https://orcid.org/0000-0003-3072-7932</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology and biochemistry, 2021-11, Vol.168, p.239-251 |
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| subjects | Anion Transport Proteins Anion Transport Proteins - genetics Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Cell Membrane Cell Membrane - metabolism Ion transport Life Sciences Mutation Nitrate Nitrate Transporters Nitrates Nitrates - metabolism Plant nutrition Plant Proteins Plant Proteins - genetics Plant Proteins - metabolism Plant Roots Plant Roots - metabolism Plant Shoots Plant Shoots - metabolism Potassium Protein Kinases Vegetal Biology |
| title | The Arabidopsis protein NPF6.2/NRT1.4 is a plasma membrane nitrate transporter and a target of protein kinase CIPK23 |
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