Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells
[Display omitted] Treatment for CNS related diseases are limited by the difficulty of the drugs to cross the blood–brain barrier (BBB). The functionalization of polymeric nanoparticles (NPs) coated with the surfactants polysorbate 80 (PS80) and poloxamer 188 (P188), have shown promising results as d...
Gespeichert in:
| Veröffentlicht in: | International journal of pharmaceutics 2022-06, Vol.621, p.121780-121780, Article 121780 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 121780 |
|---|---|
| container_issue | |
| container_start_page | 121780 |
| container_title | International journal of pharmaceutics |
| container_volume | 621 |
| creator | Moya, Elisa L.J. Lombardo, Sonia M. Vandenhaute, Elodie Schneider, Marc Mysiorek, Caroline Türeli, Akif E. Kanda, Takashi Shimizu, Fumitaka Sano, Yasuteru Maubon, Nathalie Gosselet, Fabien Günday-Türeli, Nazende Dehouck, Marie-Pierre |
| description | [Display omitted]
Treatment for CNS related diseases are limited by the difficulty of the drugs to cross the blood–brain barrier (BBB). The functionalization of polymeric nanoparticles (NPs) coated with the surfactants polysorbate 80 (PS80) and poloxamer 188 (P188), have shown promising results as drugs carriers are able to cross the BBB on animal models. In this study, poly(lactide-co-glycolide) (PLGA) NPs coated with PS80 and P188, labelled with a fluorescent dye were tested on human pre-clinical in vitro model to evaluate and compare their uptake profiles, mechanisms of transport and crossing over human brain-like endothelial cells (BLECs) mimicking the human BBB. In addition, these NPs were produced using a method facilitating their reproducible production at high scale, the MicroJet reactor® technology. Results showed that both formulations were biocompatible and able to be internalized within the BLECs in different uptake profiles depending on their coating: P188 NP showed higher internalization capacity than PS80 NP. Both NPs uptakes were ATP-dependent, following more than one endocytosis pathway with colocalization in the early endosomes, ending with a NPs release in the brain compartment. Thus, both surfactant-coated PLGA NPs are interesting formulations for delivery to the brain through the BBB, presenting different uptake profiles. |
| doi_str_mv | 10.1016/j.ijpharm.2022.121780 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_03707013v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378517322003350</els_id><sourcerecordid>2659605888</sourcerecordid><originalsourceid>FETCH-LOGICAL-c446t-864cef2168ceaf95944b6210a78a0d49bdea49d84e0c0b889a9272956ebb707a3</originalsourceid><addsrcrecordid>eNqFkU-P0zAQxS0EYsvCRwD5yB7StR0ndk6oWrF_pEpwgLM1cSaKS2IX2ynab0-qlL1yGs3oNzNP7xHykbMtZ7y-PWzd4ThAnLaCCbHlgivNXpEN16osSqnq12TDSqWLiqvyirxL6cAYqwUv35KrsqqYlEJtSPfkM0aw2QVPQ0_THPulA5-pDZCxo9_3DzvqwYcjxOzsiIn-cXmgztOTyzHQYZ7A0zaC88XofiFF34U84OhgpBbHMb0nb3oYE3641Gvy8_7rj7vHYv_t4eluty-slHUudC0t9oLX2iL0TdVI2S6CGSgNrJNN2yHIptMSmWWt1g00QommqrFtFVNQXpOb9e4AozlGN0F8NgGcedztzXm2GMIU4-WJL-znlT3G8HvGlM3k0lkteAxzMqKumppVWusFrVbUxpBSxP7lNmfmHIY5mEsY5hyGWcNY9j5dXszthN3L1j_3F-DLCuBiyslhNMk69BY7F9Fm0wX3nxd_AbJjnWo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2659605888</pqid></control><display><type>article</type><title>Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells</title><source>Elsevier ScienceDirect Journals</source><creator>Moya, Elisa L.J. ; Lombardo, Sonia M. ; Vandenhaute, Elodie ; Schneider, Marc ; Mysiorek, Caroline ; Türeli, Akif E. ; Kanda, Takashi ; Shimizu, Fumitaka ; Sano, Yasuteru ; Maubon, Nathalie ; Gosselet, Fabien ; Günday-Türeli, Nazende ; Dehouck, Marie-Pierre</creator><creatorcontrib>Moya, Elisa L.J. ; Lombardo, Sonia M. ; Vandenhaute, Elodie ; Schneider, Marc ; Mysiorek, Caroline ; Türeli, Akif E. ; Kanda, Takashi ; Shimizu, Fumitaka ; Sano, Yasuteru ; Maubon, Nathalie ; Gosselet, Fabien ; Günday-Türeli, Nazende ; Dehouck, Marie-Pierre</creatorcontrib><description>[Display omitted]
Treatment for CNS related diseases are limited by the difficulty of the drugs to cross the blood–brain barrier (BBB). The functionalization of polymeric nanoparticles (NPs) coated with the surfactants polysorbate 80 (PS80) and poloxamer 188 (P188), have shown promising results as drugs carriers are able to cross the BBB on animal models. In this study, poly(lactide-co-glycolide) (PLGA) NPs coated with PS80 and P188, labelled with a fluorescent dye were tested on human pre-clinical in vitro model to evaluate and compare their uptake profiles, mechanisms of transport and crossing over human brain-like endothelial cells (BLECs) mimicking the human BBB. In addition, these NPs were produced using a method facilitating their reproducible production at high scale, the MicroJet reactor® technology. Results showed that both formulations were biocompatible and able to be internalized within the BLECs in different uptake profiles depending on their coating: P188 NP showed higher internalization capacity than PS80 NP. Both NPs uptakes were ATP-dependent, following more than one endocytosis pathway with colocalization in the early endosomes, ending with a NPs release in the brain compartment. Thus, both surfactant-coated PLGA NPs are interesting formulations for delivery to the brain through the BBB, presenting different uptake profiles.</description><identifier>ISSN: 0378-5173</identifier><identifier>EISSN: 1873-3476</identifier><identifier>DOI: 10.1016/j.ijpharm.2022.121780</identifier><identifier>PMID: 35504427</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; BBB in vitro models ; Blood-Brain Barrier (BBB) ; Blood-Brain Barrier / metabolism ; Brain / metabolism ; Drug Carriers / metabolism ; Endothelial Cells / metabolism ; Excipients / metabolism ; Humans Nanoparticles ; Life Sciences ; Nanocarriers ; Poloxamer / metabolism ; Poloxamer 188 ; Poly(lactide-co-glycolide) nanoparticles ; Polysorbate 80 ; Polysorbates Pulmonary Surfactants / metabolism ; Surface-Active Agents / metabolism</subject><ispartof>International journal of pharmaceutics, 2022-06, Vol.621, p.121780-121780, Article 121780</ispartof><rights>2022 Université d'Artois, service facturier</rights><rights>Copyright © 2022 Université d'Artois, service facturier. Published by Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-864cef2168ceaf95944b6210a78a0d49bdea49d84e0c0b889a9272956ebb707a3</citedby><cites>FETCH-LOGICAL-c446t-864cef2168ceaf95944b6210a78a0d49bdea49d84e0c0b889a9272956ebb707a3</cites><orcidid>0000-0002-8538-5012 ; 0000-0003-2766-0052 ; 0000-0002-6310-4785 ; 0000-0001-7703-2535 ; 0000-0002-9260-7357 ; 0000-0002-9217-3202 ; 0000-0003-2338-8821 ; 0000-0002-0481-5026</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpharm.2022.121780$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35504427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://univ-artois.hal.science/hal-03707013$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Moya, Elisa L.J.</creatorcontrib><creatorcontrib>Lombardo, Sonia M.</creatorcontrib><creatorcontrib>Vandenhaute, Elodie</creatorcontrib><creatorcontrib>Schneider, Marc</creatorcontrib><creatorcontrib>Mysiorek, Caroline</creatorcontrib><creatorcontrib>Türeli, Akif E.</creatorcontrib><creatorcontrib>Kanda, Takashi</creatorcontrib><creatorcontrib>Shimizu, Fumitaka</creatorcontrib><creatorcontrib>Sano, Yasuteru</creatorcontrib><creatorcontrib>Maubon, Nathalie</creatorcontrib><creatorcontrib>Gosselet, Fabien</creatorcontrib><creatorcontrib>Günday-Türeli, Nazende</creatorcontrib><creatorcontrib>Dehouck, Marie-Pierre</creatorcontrib><title>Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells</title><title>International journal of pharmaceutics</title><addtitle>Int J Pharm</addtitle><description>[Display omitted]
Treatment for CNS related diseases are limited by the difficulty of the drugs to cross the blood–brain barrier (BBB). The functionalization of polymeric nanoparticles (NPs) coated with the surfactants polysorbate 80 (PS80) and poloxamer 188 (P188), have shown promising results as drugs carriers are able to cross the BBB on animal models. In this study, poly(lactide-co-glycolide) (PLGA) NPs coated with PS80 and P188, labelled with a fluorescent dye were tested on human pre-clinical in vitro model to evaluate and compare their uptake profiles, mechanisms of transport and crossing over human brain-like endothelial cells (BLECs) mimicking the human BBB. In addition, these NPs were produced using a method facilitating their reproducible production at high scale, the MicroJet reactor® technology. Results showed that both formulations were biocompatible and able to be internalized within the BLECs in different uptake profiles depending on their coating: P188 NP showed higher internalization capacity than PS80 NP. Both NPs uptakes were ATP-dependent, following more than one endocytosis pathway with colocalization in the early endosomes, ending with a NPs release in the brain compartment. Thus, both surfactant-coated PLGA NPs are interesting formulations for delivery to the brain through the BBB, presenting different uptake profiles.</description><subject>Animals</subject><subject>BBB in vitro models</subject><subject>Blood-Brain Barrier (BBB)</subject><subject>Blood-Brain Barrier / metabolism</subject><subject>Brain / metabolism</subject><subject>Drug Carriers / metabolism</subject><subject>Endothelial Cells / metabolism</subject><subject>Excipients / metabolism</subject><subject>Humans Nanoparticles</subject><subject>Life Sciences</subject><subject>Nanocarriers</subject><subject>Poloxamer / metabolism</subject><subject>Poloxamer 188</subject><subject>Poly(lactide-co-glycolide) nanoparticles</subject><subject>Polysorbate 80</subject><subject>Polysorbates Pulmonary Surfactants / metabolism</subject><subject>Surface-Active Agents / metabolism</subject><issn>0378-5173</issn><issn>1873-3476</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkU-P0zAQxS0EYsvCRwD5yB7StR0ndk6oWrF_pEpwgLM1cSaKS2IX2ynab0-qlL1yGs3oNzNP7xHykbMtZ7y-PWzd4ThAnLaCCbHlgivNXpEN16osSqnq12TDSqWLiqvyirxL6cAYqwUv35KrsqqYlEJtSPfkM0aw2QVPQ0_THPulA5-pDZCxo9_3DzvqwYcjxOzsiIn-cXmgztOTyzHQYZ7A0zaC88XofiFF34U84OhgpBbHMb0nb3oYE3641Gvy8_7rj7vHYv_t4eluty-slHUudC0t9oLX2iL0TdVI2S6CGSgNrJNN2yHIptMSmWWt1g00QommqrFtFVNQXpOb9e4AozlGN0F8NgGcedztzXm2GMIU4-WJL-znlT3G8HvGlM3k0lkteAxzMqKumppVWusFrVbUxpBSxP7lNmfmHIY5mEsY5hyGWcNY9j5dXszthN3L1j_3F-DLCuBiyslhNMk69BY7F9Fm0wX3nxd_AbJjnWo</recordid><startdate>20220610</startdate><enddate>20220610</enddate><creator>Moya, Elisa L.J.</creator><creator>Lombardo, Sonia M.</creator><creator>Vandenhaute, Elodie</creator><creator>Schneider, Marc</creator><creator>Mysiorek, Caroline</creator><creator>Türeli, Akif E.</creator><creator>Kanda, Takashi</creator><creator>Shimizu, Fumitaka</creator><creator>Sano, Yasuteru</creator><creator>Maubon, Nathalie</creator><creator>Gosselet, Fabien</creator><creator>Günday-Türeli, Nazende</creator><creator>Dehouck, Marie-Pierre</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-8538-5012</orcidid><orcidid>https://orcid.org/0000-0003-2766-0052</orcidid><orcidid>https://orcid.org/0000-0002-6310-4785</orcidid><orcidid>https://orcid.org/0000-0001-7703-2535</orcidid><orcidid>https://orcid.org/0000-0002-9260-7357</orcidid><orcidid>https://orcid.org/0000-0002-9217-3202</orcidid><orcidid>https://orcid.org/0000-0003-2338-8821</orcidid><orcidid>https://orcid.org/0000-0002-0481-5026</orcidid></search><sort><creationdate>20220610</creationdate><title>Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells</title><author>Moya, Elisa L.J. ; Lombardo, Sonia M. ; Vandenhaute, Elodie ; Schneider, Marc ; Mysiorek, Caroline ; Türeli, Akif E. ; Kanda, Takashi ; Shimizu, Fumitaka ; Sano, Yasuteru ; Maubon, Nathalie ; Gosselet, Fabien ; Günday-Türeli, Nazende ; Dehouck, Marie-Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-864cef2168ceaf95944b6210a78a0d49bdea49d84e0c0b889a9272956ebb707a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animals</topic><topic>BBB in vitro models</topic><topic>Blood-Brain Barrier (BBB)</topic><topic>Blood-Brain Barrier / metabolism</topic><topic>Brain / metabolism</topic><topic>Drug Carriers / metabolism</topic><topic>Endothelial Cells / metabolism</topic><topic>Excipients / metabolism</topic><topic>Humans Nanoparticles</topic><topic>Life Sciences</topic><topic>Nanocarriers</topic><topic>Poloxamer / metabolism</topic><topic>Poloxamer 188</topic><topic>Poly(lactide-co-glycolide) nanoparticles</topic><topic>Polysorbate 80</topic><topic>Polysorbates Pulmonary Surfactants / metabolism</topic><topic>Surface-Active Agents / metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moya, Elisa L.J.</creatorcontrib><creatorcontrib>Lombardo, Sonia M.</creatorcontrib><creatorcontrib>Vandenhaute, Elodie</creatorcontrib><creatorcontrib>Schneider, Marc</creatorcontrib><creatorcontrib>Mysiorek, Caroline</creatorcontrib><creatorcontrib>Türeli, Akif E.</creatorcontrib><creatorcontrib>Kanda, Takashi</creatorcontrib><creatorcontrib>Shimizu, Fumitaka</creatorcontrib><creatorcontrib>Sano, Yasuteru</creatorcontrib><creatorcontrib>Maubon, Nathalie</creatorcontrib><creatorcontrib>Gosselet, Fabien</creatorcontrib><creatorcontrib>Günday-Türeli, Nazende</creatorcontrib><creatorcontrib>Dehouck, Marie-Pierre</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>International journal of pharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moya, Elisa L.J.</au><au>Lombardo, Sonia M.</au><au>Vandenhaute, Elodie</au><au>Schneider, Marc</au><au>Mysiorek, Caroline</au><au>Türeli, Akif E.</au><au>Kanda, Takashi</au><au>Shimizu, Fumitaka</au><au>Sano, Yasuteru</au><au>Maubon, Nathalie</au><au>Gosselet, Fabien</au><au>Günday-Türeli, Nazende</au><au>Dehouck, Marie-Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells</atitle><jtitle>International journal of pharmaceutics</jtitle><addtitle>Int J Pharm</addtitle><date>2022-06-10</date><risdate>2022</risdate><volume>621</volume><spage>121780</spage><epage>121780</epage><pages>121780-121780</pages><artnum>121780</artnum><issn>0378-5173</issn><eissn>1873-3476</eissn><abstract>[Display omitted]
Treatment for CNS related diseases are limited by the difficulty of the drugs to cross the blood–brain barrier (BBB). The functionalization of polymeric nanoparticles (NPs) coated with the surfactants polysorbate 80 (PS80) and poloxamer 188 (P188), have shown promising results as drugs carriers are able to cross the BBB on animal models. In this study, poly(lactide-co-glycolide) (PLGA) NPs coated with PS80 and P188, labelled with a fluorescent dye were tested on human pre-clinical in vitro model to evaluate and compare their uptake profiles, mechanisms of transport and crossing over human brain-like endothelial cells (BLECs) mimicking the human BBB. In addition, these NPs were produced using a method facilitating their reproducible production at high scale, the MicroJet reactor® technology. Results showed that both formulations were biocompatible and able to be internalized within the BLECs in different uptake profiles depending on their coating: P188 NP showed higher internalization capacity than PS80 NP. Both NPs uptakes were ATP-dependent, following more than one endocytosis pathway with colocalization in the early endosomes, ending with a NPs release in the brain compartment. Thus, both surfactant-coated PLGA NPs are interesting formulations for delivery to the brain through the BBB, presenting different uptake profiles.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>35504427</pmid><doi>10.1016/j.ijpharm.2022.121780</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-8538-5012</orcidid><orcidid>https://orcid.org/0000-0003-2766-0052</orcidid><orcidid>https://orcid.org/0000-0002-6310-4785</orcidid><orcidid>https://orcid.org/0000-0001-7703-2535</orcidid><orcidid>https://orcid.org/0000-0002-9260-7357</orcidid><orcidid>https://orcid.org/0000-0002-9217-3202</orcidid><orcidid>https://orcid.org/0000-0003-2338-8821</orcidid><orcidid>https://orcid.org/0000-0002-0481-5026</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-5173 |
| ispartof | International journal of pharmaceutics, 2022-06, Vol.621, p.121780-121780, Article 121780 |
| issn | 0378-5173 1873-3476 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03707013v1 |
| source | Elsevier ScienceDirect Journals |
| subjects | Animals BBB in vitro models Blood-Brain Barrier (BBB) Blood-Brain Barrier / metabolism Brain / metabolism Drug Carriers / metabolism Endothelial Cells / metabolism Excipients / metabolism Humans Nanoparticles Life Sciences Nanocarriers Poloxamer / metabolism Poloxamer 188 Poly(lactide-co-glycolide) nanoparticles Polysorbate 80 Polysorbates Pulmonary Surfactants / metabolism Surface-Active Agents / metabolism |
| title | Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T16%3A14%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Interaction%20of%20surfactant%20coated%20PLGA%20nanoparticles%20with%20in%20vitro%20human%20brain-like%20endothelial%20cells&rft.jtitle=International%20journal%20of%20pharmaceutics&rft.au=Moya,%20Elisa%20L.J.&rft.date=2022-06-10&rft.volume=621&rft.spage=121780&rft.epage=121780&rft.pages=121780-121780&rft.artnum=121780&rft.issn=0378-5173&rft.eissn=1873-3476&rft_id=info:doi/10.1016/j.ijpharm.2022.121780&rft_dat=%3Cproquest_hal_p%3E2659605888%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2659605888&rft_id=info:pmid/35504427&rft_els_id=S0378517322003350&rfr_iscdi=true |