Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation

Poly(ethylene glycol) diglycidyl ether (PEGDE) is widely used as an additive for cross-linking polymers bearing amine, hydroxyl, or carboxyl groups. However, the idea of using PEGDE alone for immobilizing proteins on biosensors has never been thoroughly explored. We report the successful fabrication...

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Veröffentlicht in:	Biosensors & bioelectronics 2011-06, Vol.26 (10), p.3993-4000
Hauptverfasser:	Vasylieva, Natalia, Barnych, Bogdan, Meiller, Anne, Maucler, Caroline, Pollegioni, Loredano, Lin, Jian-Sheng, Barbier, Daniel, Marinesco, Stéphane
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Oxidoreductases Amperometry Animals Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Brain Chemistry Cross-Linking Reagents D-Amino-Acid Oxidase d-Serine Enzymatic electrode Enzymes, Immobilized Epoxy Resins Fixatives Fundamental and applied biological sciences. Psychology General aspects Glucose Glucose - analysis Glucose Oxidase Glutamate Glutamic Acid - analysis Glutaral Immobilization techniques In vivo monitoring Life Sciences Male Methods. Procedures. Technologies Rats Rats, Wistar Serine - analysis Various methods and equipments
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