Poly- and Oligosaccharide Ulva sp. Fractions from Enzyme-Assisted Extraction Modulate the Metabolism of Extracellular Matrix in Human Skin Fibroblasts: Potential in Anti-Aging Dermo-Cosmetic Applications
sp. is known to be a source of bioactive compounds such as ulvans, but their biological activity on human dermal fibroblast extracellular matrix (ECM) is poorly reported. In this work, the regulation of ECM has been investigated for the first time at both proteomic and transcriptomic levels in norma...
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| Veröffentlicht in: | Marine drugs 2021-03, Vol.19 (3), p.156 |
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| creator | Fournière, Mathilde Bedoux, Gilles Lebonvallet, Nicolas Leschiera, Raphaël Le Goff-Pain, Claudie Bourgougnon, Nathalie Latire, Thomas |
| description | sp. is known to be a source of bioactive compounds such as ulvans, but their biological activity on human dermal fibroblast extracellular matrix (ECM) is poorly reported. In this work, the regulation of ECM has been investigated for the first time at both proteomic and transcriptomic levels in normal human skin dermal fibroblasts, after 48 h of incubation with poly- and oligosaccharide fractions from
sp. obtained after enzyme-assisted extraction and depolymerization. Cell proliferation enhancement (up to +68%) without exhibiting any cytotoxic effect on fibroblasts was demonstrated at 50 and 1000 µg/mL by both fractions. At the proteomic level, polysaccharide fractions at 1000 µg/mL enhanced the most the synthesis of glycosaminoglycans (GAGs, up to +57%), total collagen, especially types I (up to +217%) and III, as well as the synthesis and activity of MMP-1 (Matrix Metalloproteinase-1, up to +309%). In contrast, oligosaccharide fractions had no effect on GAGs synthesis but exhibited similarities for collagens and MMP-1 regulation. At the transcriptomic level, the decrease of
and
expression, and increase of
and
expression, confirmed the modulation of ECM metabolism by both fractions. Our research emphasizes that poly- and oligosaccharide
sp. fractions exhibit interesting biological activities and supports their potential use in the area of skin renewal for anti-aging dermo-cosmetic applications. |
| doi_str_mv | 10.3390/md19030156 |
| format | Article |
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sp. obtained after enzyme-assisted extraction and depolymerization. Cell proliferation enhancement (up to +68%) without exhibiting any cytotoxic effect on fibroblasts was demonstrated at 50 and 1000 µg/mL by both fractions. At the proteomic level, polysaccharide fractions at 1000 µg/mL enhanced the most the synthesis of glycosaminoglycans (GAGs, up to +57%), total collagen, especially types I (up to +217%) and III, as well as the synthesis and activity of MMP-1 (Matrix Metalloproteinase-1, up to +309%). In contrast, oligosaccharide fractions had no effect on GAGs synthesis but exhibited similarities for collagens and MMP-1 regulation. At the transcriptomic level, the decrease of
and
expression, and increase of
and
expression, confirmed the modulation of ECM metabolism by both fractions. Our research emphasizes that poly- and oligosaccharide
sp. fractions exhibit interesting biological activities and supports their potential use in the area of skin renewal for anti-aging dermo-cosmetic applications.</description><identifier>ISSN: 1660-3397</identifier><identifier>EISSN: 1660-3397</identifier><identifier>DOI: 10.3390/md19030156</identifier><identifier>PMID: 33802739</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Acids ; Ageing ; Aging ; Algae ; Analytical chemistry ; Bioactive compounds ; Biological activity ; Carbohydrates ; Cell proliferation ; Chemical Sciences ; Clean technology ; Collagen ; Collagen (type I) ; Cosmetics ; Cytotoxicity ; Depolymerization ; Enzymes ; Extracellular ; Extracellular matrix ; Fibroblasts ; Glycosaminoglycans ; Heparan sulfate ; human dermal fibroblast ; Incubation period ; Interstitial collagenase ; Life Sciences ; Matrix metalloproteinase ; Matrix metalloproteinases ; Metabolism ; Metalloproteinase ; Molecular weight ; Oligosaccharides ; Polysaccharides ; Proliferation ; Proteins ; Proteomics ; Renewal ; seaweed ; Skin ; Synthesis ; Ulva sp</subject><ispartof>Marine drugs, 2021-03, Vol.19 (3), p.156</ispartof><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c506t-ee1c8b872d34198a7e134dc1b3921be23452c70112e92f2bb68eb4cc6c3913da3</citedby><cites>FETCH-LOGICAL-c506t-ee1c8b872d34198a7e134dc1b3921be23452c70112e92f2bb68eb4cc6c3913da3</cites><orcidid>0000-0002-5270-2325 ; 0000-0001-9780-6067 ; 0000-0003-4108-1073 ; 0000-0001-7755-3357</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002389/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8002389/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2095,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33802739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://ubs.hal.science/hal-03639068$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Fournière, Mathilde</creatorcontrib><creatorcontrib>Bedoux, Gilles</creatorcontrib><creatorcontrib>Lebonvallet, Nicolas</creatorcontrib><creatorcontrib>Leschiera, Raphaël</creatorcontrib><creatorcontrib>Le Goff-Pain, Claudie</creatorcontrib><creatorcontrib>Bourgougnon, Nathalie</creatorcontrib><creatorcontrib>Latire, Thomas</creatorcontrib><title>Poly- and Oligosaccharide Ulva sp. Fractions from Enzyme-Assisted Extraction Modulate the Metabolism of Extracellular Matrix in Human Skin Fibroblasts: Potential in Anti-Aging Dermo-Cosmetic Applications</title><title>Marine drugs</title><addtitle>Mar Drugs</addtitle><description>sp. is known to be a source of bioactive compounds such as ulvans, but their biological activity on human dermal fibroblast extracellular matrix (ECM) is poorly reported. In this work, the regulation of ECM has been investigated for the first time at both proteomic and transcriptomic levels in normal human skin dermal fibroblasts, after 48 h of incubation with poly- and oligosaccharide fractions from
sp. obtained after enzyme-assisted extraction and depolymerization. Cell proliferation enhancement (up to +68%) without exhibiting any cytotoxic effect on fibroblasts was demonstrated at 50 and 1000 µg/mL by both fractions. At the proteomic level, polysaccharide fractions at 1000 µg/mL enhanced the most the synthesis of glycosaminoglycans (GAGs, up to +57%), total collagen, especially types I (up to +217%) and III, as well as the synthesis and activity of MMP-1 (Matrix Metalloproteinase-1, up to +309%). In contrast, oligosaccharide fractions had no effect on GAGs synthesis but exhibited similarities for collagens and MMP-1 regulation. At the transcriptomic level, the decrease of
and
expression, and increase of
and
expression, confirmed the modulation of ECM metabolism by both fractions. Our research emphasizes that poly- and oligosaccharide
sp. fractions exhibit interesting biological activities and supports their potential use in the area of skin renewal for anti-aging dermo-cosmetic applications.</description><subject>Acids</subject><subject>Ageing</subject><subject>Aging</subject><subject>Algae</subject><subject>Analytical chemistry</subject><subject>Bioactive compounds</subject><subject>Biological activity</subject><subject>Carbohydrates</subject><subject>Cell proliferation</subject><subject>Chemical Sciences</subject><subject>Clean technology</subject><subject>Collagen</subject><subject>Collagen (type I)</subject><subject>Cosmetics</subject><subject>Cytotoxicity</subject><subject>Depolymerization</subject><subject>Enzymes</subject><subject>Extracellular</subject><subject>Extracellular matrix</subject><subject>Fibroblasts</subject><subject>Glycosaminoglycans</subject><subject>Heparan sulfate</subject><subject>human dermal fibroblast</subject><subject>Incubation period</subject><subject>Interstitial collagenase</subject><subject>Life Sciences</subject><subject>Matrix metalloproteinase</subject><subject>Matrix metalloproteinases</subject><subject>Metabolism</subject><subject>Metalloproteinase</subject><subject>Molecular weight</subject><subject>Oligosaccharides</subject><subject>Polysaccharides</subject><subject>Proliferation</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Renewal</subject><subject>seaweed</subject><subject>Skin</subject><subject>Synthesis</subject><subject>Ulva sp</subject><issn>1660-3397</issn><issn>1660-3397</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNpdks9uEzEQxlcIREvhwgMgS1wAaYu93r89IK1CQiolaiXo2Zr1ziYO3nWwnajhFXkpnCaUtiePZn7-Zsb-ougto-ecV_Rz37KKcsqy_Fl0yvKcxiFdPH8Qn0SvnFtRyrOySl9GJ5yXNCl4dRr9uTZ6FxMYWnKl1cI4kHIJVrVIbvQWiFufk4kF6ZUZHOms6cl4-L3rMa6dU85jS8a3_giQuWk3GjwSv0QyRw-N0cr1xHRHCrUOgCVz8FbdEjWQ6aaHgXz_GcKJaqxpNDjvLsi18Th4BXoP1SGK64UaFuQr2t7EI-N69EqSer3WSsLdeK-jFx1oh2-O51l0Mxn_GE3j2dW3y1E9i2VGcx8jMlk2ZZG0PGVVCQUynraSNbxKWIMJT7NEFpSxBKukS5omL7FJpcwlrxhvgZ9Flwfd1sBKrK3qwe6EASXuEsYuBNgwnEaRFkXW5kWW0DwLzbqm64oUkFHouqzqaND6ctBab5oeWxl2tqAfiT6uDGopFmYrSkoTXlZB4ONBYPnk2rSeiX2O8jyYJC-3LLAfjs2s-bVB50Wv3P5PYECzcSLJaJmVjGdFQN8_QVdmY4fwrHuKB82U5oH6dKCkNc5Z7O4nYFTsvSn-ezPA7x6ueo_-MyP_Czap4ZU</recordid><startdate>20210317</startdate><enddate>20210317</enddate><creator>Fournière, Mathilde</creator><creator>Bedoux, Gilles</creator><creator>Lebonvallet, Nicolas</creator><creator>Leschiera, Raphaël</creator><creator>Le Goff-Pain, Claudie</creator><creator>Bourgougnon, Nathalie</creator><creator>Latire, Thomas</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7TN</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-5270-2325</orcidid><orcidid>https://orcid.org/0000-0001-9780-6067</orcidid><orcidid>https://orcid.org/0000-0003-4108-1073</orcidid><orcidid>https://orcid.org/0000-0001-7755-3357</orcidid></search><sort><creationdate>20210317</creationdate><title>Poly- and Oligosaccharide Ulva sp. Fractions from Enzyme-Assisted Extraction Modulate the Metabolism of Extracellular Matrix in Human Skin Fibroblasts: Potential in Anti-Aging Dermo-Cosmetic Applications</title><author>Fournière, Mathilde ; Bedoux, Gilles ; Lebonvallet, Nicolas ; Leschiera, Raphaël ; Le Goff-Pain, Claudie ; Bourgougnon, Nathalie ; Latire, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-ee1c8b872d34198a7e134dc1b3921be23452c70112e92f2bb68eb4cc6c3913da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Acids</topic><topic>Ageing</topic><topic>Aging</topic><topic>Algae</topic><topic>Analytical chemistry</topic><topic>Bioactive compounds</topic><topic>Biological activity</topic><topic>Carbohydrates</topic><topic>Cell proliferation</topic><topic>Chemical Sciences</topic><topic>Clean technology</topic><topic>Collagen</topic><topic>Collagen (type 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Gilles</creatorcontrib><creatorcontrib>Lebonvallet, Nicolas</creatorcontrib><creatorcontrib>Leschiera, Raphaël</creatorcontrib><creatorcontrib>Le Goff-Pain, Claudie</creatorcontrib><creatorcontrib>Bourgougnon, Nathalie</creatorcontrib><creatorcontrib>Latire, Thomas</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni 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Fractions from Enzyme-Assisted Extraction Modulate the Metabolism of Extracellular Matrix in Human Skin Fibroblasts: Potential in Anti-Aging Dermo-Cosmetic Applications</atitle><jtitle>Marine drugs</jtitle><addtitle>Mar Drugs</addtitle><date>2021-03-17</date><risdate>2021</risdate><volume>19</volume><issue>3</issue><spage>156</spage><pages>156-</pages><issn>1660-3397</issn><eissn>1660-3397</eissn><abstract>sp. is known to be a source of bioactive compounds such as ulvans, but their biological activity on human dermal fibroblast extracellular matrix (ECM) is poorly reported. In this work, the regulation of ECM has been investigated for the first time at both proteomic and transcriptomic levels in normal human skin dermal fibroblasts, after 48 h of incubation with poly- and oligosaccharide fractions from
sp. obtained after enzyme-assisted extraction and depolymerization. Cell proliferation enhancement (up to +68%) without exhibiting any cytotoxic effect on fibroblasts was demonstrated at 50 and 1000 µg/mL by both fractions. At the proteomic level, polysaccharide fractions at 1000 µg/mL enhanced the most the synthesis of glycosaminoglycans (GAGs, up to +57%), total collagen, especially types I (up to +217%) and III, as well as the synthesis and activity of MMP-1 (Matrix Metalloproteinase-1, up to +309%). In contrast, oligosaccharide fractions had no effect on GAGs synthesis but exhibited similarities for collagens and MMP-1 regulation. At the transcriptomic level, the decrease of
and
expression, and increase of
and
expression, confirmed the modulation of ECM metabolism by both fractions. Our research emphasizes that poly- and oligosaccharide
sp. fractions exhibit interesting biological activities and supports their potential use in the area of skin renewal for anti-aging dermo-cosmetic applications.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33802739</pmid><doi>10.3390/md19030156</doi><orcidid>https://orcid.org/0000-0002-5270-2325</orcidid><orcidid>https://orcid.org/0000-0001-9780-6067</orcidid><orcidid>https://orcid.org/0000-0003-4108-1073</orcidid><orcidid>https://orcid.org/0000-0001-7755-3357</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Acids Ageing Aging Algae Analytical chemistry Bioactive compounds Biological activity Carbohydrates Cell proliferation Chemical Sciences Clean technology Collagen Collagen (type I) Cosmetics Cytotoxicity Depolymerization Enzymes Extracellular Extracellular matrix Fibroblasts Glycosaminoglycans Heparan sulfate human dermal fibroblast Incubation period Interstitial collagenase Life Sciences Matrix metalloproteinase Matrix metalloproteinases Metabolism Metalloproteinase Molecular weight Oligosaccharides Polysaccharides Proliferation Proteins Proteomics Renewal seaweed Skin Synthesis Ulva sp |
| title | Poly- and Oligosaccharide Ulva sp. Fractions from Enzyme-Assisted Extraction Modulate the Metabolism of Extracellular Matrix in Human Skin Fibroblasts: Potential in Anti-Aging Dermo-Cosmetic Applications |
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