Genetic and biocatalytic basis of formate dependent growth of Escherichia coli strains evolved in continuous culture

The reductive glycine pathway was described as the most energetically favorable synthetic route of aerobic formate assimilation. Here we report the successful implementation of formatotrophy in Escherichia coli by means of a stepwise adaptive evolution strategy. Medium swap and turbidostat regimes o...

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Veröffentlicht in:	Metabolic engineering 2022-07, Vol.72, p.200-214
Hauptverfasser:	Delmas, Valérie A., Perchat, Nadia, Monet, Oriane, Fouré, Marion, Darii, Ekatarina, Roche, David, Dubois, Ivan, Pateau, Emilie, Perret, Alain, Döring, Volker, Bouzon, Madeleine
Format:	Artikel
Sprache:	eng
Schlagworte:	Continuous culture Escherichia coli Evolution Formate assimilation Life Sciences Lipoamide dehydrogenase Methylene-H4F dehydrogenase/cyclohydrolase Mutation analysis One carbon metabolism
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