Identification and expression of a CHMO from the Pseudomonas aeruginosa strain Pa1242: application to the bioconversion of Cyrene™ into a key precursor ( S )-γ-hydroxymethyl-butyrolactone
A sequence from the complete genome of the Pseudomonas aeruginosa strain Pa1242 was identified to code for a potentially new cyclohexanone monooxygenase (CHMO). The latter was discovered using the Basic Alignment Search Tool (BLAST) with the DNA sequence of the CHMO from Acinetobacter sp. NCIB 9871...
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| Veröffentlicht in: | Green chemistry : an international journal and green chemistry resource : GC 2021-04, Vol.23 (7), p.2694-2702 |
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| container_title | Green chemistry : an international journal and green chemistry resource : GC |
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| creator | Mouterde, Louis M. M. Couvreur, Julien Langlait, Maxime M. J. Brunois, Fanny Allais, Florent |
| description | A sequence from the complete genome of the
Pseudomonas aeruginosa
strain Pa1242 was identified to code for a potentially new cyclohexanone monooxygenase (CHMO). The latter was discovered using the Basic Alignment Search Tool (BLAST) with the DNA sequence of the CHMO from
Acinetobacter
sp. NCIB 9871 as the parent sequence considering that this enzyme family maintains a relatively high query cover (>95%) and identity percentage (>50%) among many different microorganisms. This was confirmed by the bioconversion of the cellulose-based green solvent Cyrene™ into a key precursor (
S
)-γ-hydroxymethyl-butyrolactone (
2H-HBO
) using a new strain of
E. coli
BL21(DE3) containing a plasmid designed for the overexpression of the unprecedented CHMO (pLM7). Besides confirming the CHMO activity of this novel sequence, this bioconversion allows the obtention of
2H-HBO
while maintaining the naturalness of the process. The optimal culture conditions were assessed through a Design of Experiments (DoE), and the control of the pH and O
2
level allowed to reach a working concentration of 20 g L
−1
of Cyrene™ with total conversion and a productivity rate of 401 ± 36 mg L
−1
h
−1
. |
| doi_str_mv | 10.1039/D0GC04321D |
| format | Article |
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Pseudomonas aeruginosa
strain Pa1242 was identified to code for a potentially new cyclohexanone monooxygenase (CHMO). The latter was discovered using the Basic Alignment Search Tool (BLAST) with the DNA sequence of the CHMO from
Acinetobacter
sp. NCIB 9871 as the parent sequence considering that this enzyme family maintains a relatively high query cover (>95%) and identity percentage (>50%) among many different microorganisms. This was confirmed by the bioconversion of the cellulose-based green solvent Cyrene™ into a key precursor (
S
)-γ-hydroxymethyl-butyrolactone (
2H-HBO
) using a new strain of
E. coli
BL21(DE3) containing a plasmid designed for the overexpression of the unprecedented CHMO (pLM7). Besides confirming the CHMO activity of this novel sequence, this bioconversion allows the obtention of
2H-HBO
while maintaining the naturalness of the process. The optimal culture conditions were assessed through a Design of Experiments (DoE), and the control of the pH and O
2
level allowed to reach a working concentration of 20 g L
−1
of Cyrene™ with total conversion and a productivity rate of 401 ± 36 mg L
−1
h
−1
.</description><identifier>ISSN: 1463-9262</identifier><identifier>EISSN: 1463-9270</identifier><identifier>DOI: 10.1039/D0GC04321D</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Bioconversion ; Biotechnology ; Butyrolactone ; Cell culture ; Cellulose ; Cyclohexanone ; Cyclohexanone monooxygenase ; Deoxyribonucleic acid ; Design of experiments ; DNA ; E coli ; Genomes ; Green chemistry ; Life Sciences ; Mass spectroscopy ; Microorganisms ; NMR ; Nuclear magnetic resonance ; Nucleotide sequence ; Precursors ; Pseudomonas aeruginosa</subject><ispartof>Green chemistry : an international journal and green chemistry resource : GC, 2021-04, Vol.23 (7), p.2694-2702</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c223t-45e385703d7f970b655c539598906ad1854918662e3be1cc6881b5434e7f438f3</citedby><cites>FETCH-LOGICAL-c223t-45e385703d7f970b655c539598906ad1854918662e3be1cc6881b5434e7f438f3</cites><orcidid>0000-0002-4096-1629 ; 0000-0003-4132-6210 ; 0000-0002-6532-0668</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://agroparistech.hal.science/hal-03591042$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Mouterde, Louis M. M.</creatorcontrib><creatorcontrib>Couvreur, Julien</creatorcontrib><creatorcontrib>Langlait, Maxime M. J.</creatorcontrib><creatorcontrib>Brunois, Fanny</creatorcontrib><creatorcontrib>Allais, Florent</creatorcontrib><title>Identification and expression of a CHMO from the Pseudomonas aeruginosa strain Pa1242: application to the bioconversion of Cyrene™ into a key precursor ( S )-γ-hydroxymethyl-butyrolactone</title><title>Green chemistry : an international journal and green chemistry resource : GC</title><description>A sequence from the complete genome of the
Pseudomonas aeruginosa
strain Pa1242 was identified to code for a potentially new cyclohexanone monooxygenase (CHMO). The latter was discovered using the Basic Alignment Search Tool (BLAST) with the DNA sequence of the CHMO from
Acinetobacter
sp. NCIB 9871 as the parent sequence considering that this enzyme family maintains a relatively high query cover (>95%) and identity percentage (>50%) among many different microorganisms. This was confirmed by the bioconversion of the cellulose-based green solvent Cyrene™ into a key precursor (
S
)-γ-hydroxymethyl-butyrolactone (
2H-HBO
) using a new strain of
E. coli
BL21(DE3) containing a plasmid designed for the overexpression of the unprecedented CHMO (pLM7). Besides confirming the CHMO activity of this novel sequence, this bioconversion allows the obtention of
2H-HBO
while maintaining the naturalness of the process. The optimal culture conditions were assessed through a Design of Experiments (DoE), and the control of the pH and O
2
level allowed to reach a working concentration of 20 g L
−1
of Cyrene™ with total conversion and a productivity rate of 401 ± 36 mg L
−1
h
−1
.</description><subject>Bioconversion</subject><subject>Biotechnology</subject><subject>Butyrolactone</subject><subject>Cell culture</subject><subject>Cellulose</subject><subject>Cyclohexanone</subject><subject>Cyclohexanone monooxygenase</subject><subject>Deoxyribonucleic acid</subject><subject>Design of experiments</subject><subject>DNA</subject><subject>E coli</subject><subject>Genomes</subject><subject>Green chemistry</subject><subject>Life Sciences</subject><subject>Mass spectroscopy</subject><subject>Microorganisms</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Nucleotide sequence</subject><subject>Precursors</subject><subject>Pseudomonas aeruginosa</subject><issn>1463-9262</issn><issn>1463-9270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpFkUtu1EAQhi0EEiGw4QQtsSGRDP203ewiBzKRBiUSsLba7TLTwdNluttRvOckXCH7HIFDcBI8DAlSSfXQV3-V9GfZS0bfMCr021N6VlMpODt9lB0wWYhc85I-fqgL_jR7FuMVpYyVhTzI7s478Mn1zprk0BPjOwI3Y4AYdy32xJB69fGC9AG3JG2AXEaYOtyiN5EYCNNX5zEaElMwzpNLw7jk74gZx-FeM-HfxdahRX8N4V65ngN4-P3jJ3F-YQz5BjNZTtspRAzkNflEjvJft_lm7gLezFtIm3nI2ynNAQdjE3p4nj3pzRDhxb98mH358P5zvcrXF2fn9ck6t5yLlEsFolIlFV3Z65K2hVJWCa10pWlhOlYpqVlVFBxEC8zaoqpYq6SQUPZSVL04zI72uhszNGNwWxPmBo1rVifrZjejQmlGJb9mC_tqz44Bv08QU3OFU_DLew1XjOklhFyo4z1lA8YYoH-QZbTZedn891L8Af7TkyQ</recordid><startdate>20210413</startdate><enddate>20210413</enddate><creator>Mouterde, Louis M. 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M.</creatorcontrib><creatorcontrib>Couvreur, Julien</creatorcontrib><creatorcontrib>Langlait, Maxime M. J.</creatorcontrib><creatorcontrib>Brunois, Fanny</creatorcontrib><creatorcontrib>Allais, Florent</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Materials Research Database</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mouterde, Louis M. M.</au><au>Couvreur, Julien</au><au>Langlait, Maxime M. J.</au><au>Brunois, Fanny</au><au>Allais, Florent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and expression of a CHMO from the Pseudomonas aeruginosa strain Pa1242: application to the bioconversion of Cyrene™ into a key precursor ( S )-γ-hydroxymethyl-butyrolactone</atitle><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle><date>2021-04-13</date><risdate>2021</risdate><volume>23</volume><issue>7</issue><spage>2694</spage><epage>2702</epage><pages>2694-2702</pages><issn>1463-9262</issn><eissn>1463-9270</eissn><abstract>A sequence from the complete genome of the
Pseudomonas aeruginosa
strain Pa1242 was identified to code for a potentially new cyclohexanone monooxygenase (CHMO). The latter was discovered using the Basic Alignment Search Tool (BLAST) with the DNA sequence of the CHMO from
Acinetobacter
sp. NCIB 9871 as the parent sequence considering that this enzyme family maintains a relatively high query cover (>95%) and identity percentage (>50%) among many different microorganisms. This was confirmed by the bioconversion of the cellulose-based green solvent Cyrene™ into a key precursor (
S
)-γ-hydroxymethyl-butyrolactone (
2H-HBO
) using a new strain of
E. coli
BL21(DE3) containing a plasmid designed for the overexpression of the unprecedented CHMO (pLM7). Besides confirming the CHMO activity of this novel sequence, this bioconversion allows the obtention of
2H-HBO
while maintaining the naturalness of the process. The optimal culture conditions were assessed through a Design of Experiments (DoE), and the control of the pH and O
2
level allowed to reach a working concentration of 20 g L
−1
of Cyrene™ with total conversion and a productivity rate of 401 ± 36 mg L
−1
h
−1
.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/D0GC04321D</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-4096-1629</orcidid><orcidid>https://orcid.org/0000-0003-4132-6210</orcidid><orcidid>https://orcid.org/0000-0002-6532-0668</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1463-9262 |
| ispartof | Green chemistry : an international journal and green chemistry resource : GC, 2021-04, Vol.23 (7), p.2694-2702 |
| issn | 1463-9262 1463-9270 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03591042v1 |
| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Bioconversion Biotechnology Butyrolactone Cell culture Cellulose Cyclohexanone Cyclohexanone monooxygenase Deoxyribonucleic acid Design of experiments DNA E coli Genomes Green chemistry Life Sciences Mass spectroscopy Microorganisms NMR Nuclear magnetic resonance Nucleotide sequence Precursors Pseudomonas aeruginosa |
| title | Identification and expression of a CHMO from the Pseudomonas aeruginosa strain Pa1242: application to the bioconversion of Cyrene™ into a key precursor ( S )-γ-hydroxymethyl-butyrolactone |
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