Osmoporation is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus
The objective of this study was to evaluate the performance of Lactobacillus acidophilus cells as a novel encapsulating carrier for fisetin via osmoporation. Initially, the effects of osmotic pressure and initial fisetin concentration on the performance of the osmoporation process were evaluated. Th...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2022-02, Vol.106 (3), p.1031-1044 |
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| creator | de Andrade, Eduardo Wagner Vasconcelos Dupont, Sebastien Beney, Laurent Hoskin, Roberta Targino da Silva Pedrini, Márcia Regina |
| description | The objective of this study was to evaluate the performance of
Lactobacillus acidophilus
cells as a novel encapsulating carrier for fisetin via osmoporation. Initially, the effects of osmotic pressure and initial fisetin concentration on the performance of the osmoporation process were evaluated. The best results were achieved when 15 MPa was applied, while the maximum loading capacity was reached when fisetin concentration of 2.0 mg·mL
−1
was used. For these conditions, the cell viability, encapsulation efficiency (EE), and encapsulated fisetin content (EF) were 72%, 28%, and 0.990 mg, respectively. Further, the encapsulation was confirmed by Fourier transform-infrared (FT-IR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) analysis. DSC thermograms revealed an increase of 40 °C in the melting point of fisetin after encapsulation. In addition, the enhancement of fisetin bioaccessibility by osmoporated biocapsules is shown for the first time in the literature. When the fisetin biocapsules were subjected to in vitro gastrointestinal digestion, 99.6% of the encapsulated content were retained through the gastric stage and 45.5% were released during the intestinal stage, despite no active cells were detected during simulated digestion. These results suggest that alive cells are required for an effective osmoporation-assisted encapsulation process; however, osmoporated biocapsules can efficiently protect and preserve labile compounds, independently of their activity. Overall, this study demonstrated that osmoporation using probiotic
L. acidophilus
is a simple, versatile, and efficient technique to encapsulate and deliver lipophilic fisetin for food applications.
Key points
•Fisetin is efficiently encapsulated into L. acidophilus via osmoporation.
•Fisetin bioaccessibility is improved by osmoporation into L. acidophilus.
•Release mechanisms of osmoporation carriers are independent of the cell activity. |
| doi_str_mv | 10.1007/s00253-021-11735-8 |
| format | Article |
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Lactobacillus acidophilus
cells as a novel encapsulating carrier for fisetin via osmoporation. Initially, the effects of osmotic pressure and initial fisetin concentration on the performance of the osmoporation process were evaluated. The best results were achieved when 15 MPa was applied, while the maximum loading capacity was reached when fisetin concentration of 2.0 mg·mL
−1
was used. For these conditions, the cell viability, encapsulation efficiency (EE), and encapsulated fisetin content (EF) were 72%, 28%, and 0.990 mg, respectively. Further, the encapsulation was confirmed by Fourier transform-infrared (FT-IR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) analysis. DSC thermograms revealed an increase of 40 °C in the melting point of fisetin after encapsulation. In addition, the enhancement of fisetin bioaccessibility by osmoporated biocapsules is shown for the first time in the literature. When the fisetin biocapsules were subjected to in vitro gastrointestinal digestion, 99.6% of the encapsulated content were retained through the gastric stage and 45.5% were released during the intestinal stage, despite no active cells were detected during simulated digestion. These results suggest that alive cells are required for an effective osmoporation-assisted encapsulation process; however, osmoporated biocapsules can efficiently protect and preserve labile compounds, independently of their activity. Overall, this study demonstrated that osmoporation using probiotic
L. acidophilus
is a simple, versatile, and efficient technique to encapsulate and deliver lipophilic fisetin for food applications.
Key points
•Fisetin is efficiently encapsulated into L. acidophilus via osmoporation.
•Fisetin bioaccessibility is improved by osmoporation into L. acidophilus.
•Release mechanisms of osmoporation carriers are independent of the cell activity.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-021-11735-8</identifier><identifier>PMID: 35024920</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Bioavailability ; Biochemistry, Molecular Biology ; Bioflavonoids ; Biomedical and Life Sciences ; Biotechnological Products and Process Engineering ; Biotechnology ; Calorimetry ; Cell viability ; Differential scanning calorimetry ; Diffraction ; Digestion ; Digestive system ; Electronic equipment and supplies ; Encapsulation ; Flavones ; Flavonoids ; Flavonols ; Fourier analysis ; Fourier transform infrared spectroscopy ; Fourier transforms ; Gastrointestinal tract ; Identification and classification ; Infrared analysis ; Lactobacilli ; Lactobacillus ; Lactobacillus acidophilus ; Life Sciences ; Lipophilic ; Melting point ; Melting points ; Microbial Genetics and Genomics ; Microbiology ; Osmosis ; Osmotic pressure ; Performance evaluation ; Plastic embedment ; Pressure effects ; Probiotics ; Spectroscopy, Fourier Transform Infrared ; X-ray diffraction ; X-rays</subject><ispartof>Applied microbiology and biotechnology, 2022-02, Vol.106 (3), p.1031-1044</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><rights>COPYRIGHT 2022 Springer</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-4fffb3db52d7f030e00eebd18e4d42cbb8310452cb24dcabb70b063d377715563</citedby><cites>FETCH-LOGICAL-c406t-4fffb3db52d7f030e00eebd18e4d42cbb8310452cb24dcabb70b063d377715563</cites><orcidid>0000-0002-3737-3500 ; 0000-0003-0900-4983 ; 0000-0001-6293-9636 ; 0000-0002-2757-2506</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-021-11735-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-021-11735-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,777,781,882,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35024920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://institut-agro-dijon.hal.science/hal-03559567$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>de Andrade, Eduardo Wagner Vasconcelos</creatorcontrib><creatorcontrib>Dupont, Sebastien</creatorcontrib><creatorcontrib>Beney, Laurent</creatorcontrib><creatorcontrib>Hoskin, Roberta Targino</creatorcontrib><creatorcontrib>da Silva Pedrini, Márcia Regina</creatorcontrib><title>Osmoporation is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>The objective of this study was to evaluate the performance of
Lactobacillus acidophilus
cells as a novel encapsulating carrier for fisetin via osmoporation. Initially, the effects of osmotic pressure and initial fisetin concentration on the performance of the osmoporation process were evaluated. The best results were achieved when 15 MPa was applied, while the maximum loading capacity was reached when fisetin concentration of 2.0 mg·mL
−1
was used. For these conditions, the cell viability, encapsulation efficiency (EE), and encapsulated fisetin content (EF) were 72%, 28%, and 0.990 mg, respectively. Further, the encapsulation was confirmed by Fourier transform-infrared (FT-IR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) analysis. DSC thermograms revealed an increase of 40 °C in the melting point of fisetin after encapsulation. In addition, the enhancement of fisetin bioaccessibility by osmoporated biocapsules is shown for the first time in the literature. When the fisetin biocapsules were subjected to in vitro gastrointestinal digestion, 99.6% of the encapsulated content were retained through the gastric stage and 45.5% were released during the intestinal stage, despite no active cells were detected during simulated digestion. These results suggest that alive cells are required for an effective osmoporation-assisted encapsulation process; however, osmoporated biocapsules can efficiently protect and preserve labile compounds, independently of their activity. Overall, this study demonstrated that osmoporation using probiotic
L. acidophilus
is a simple, versatile, and efficient technique to encapsulate and deliver lipophilic fisetin for food applications.
Key points
•Fisetin is efficiently encapsulated into L. acidophilus via osmoporation.
•Fisetin bioaccessibility is improved by osmoporation into L. acidophilus.
•Release mechanisms of osmoporation carriers are independent of the cell activity.</description><subject>Analysis</subject><subject>Bioavailability</subject><subject>Biochemistry, Molecular Biology</subject><subject>Bioflavonoids</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnological Products and Process Engineering</subject><subject>Biotechnology</subject><subject>Calorimetry</subject><subject>Cell viability</subject><subject>Differential scanning calorimetry</subject><subject>Diffraction</subject><subject>Digestion</subject><subject>Digestive system</subject><subject>Electronic equipment and supplies</subject><subject>Encapsulation</subject><subject>Flavones</subject><subject>Flavonoids</subject><subject>Flavonols</subject><subject>Fourier analysis</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Fourier transforms</subject><subject>Gastrointestinal tract</subject><subject>Identification and classification</subject><subject>Infrared analysis</subject><subject>Lactobacilli</subject><subject>Lactobacillus</subject><subject>Lactobacillus acidophilus</subject><subject>Life Sciences</subject><subject>Lipophilic</subject><subject>Melting point</subject><subject>Melting points</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Osmosis</subject><subject>Osmotic pressure</subject><subject>Performance evaluation</subject><subject>Plastic embedment</subject><subject>Pressure effects</subject><subject>Probiotics</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>X-ray 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is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus</title><author>de Andrade, Eduardo Wagner Vasconcelos ; Dupont, Sebastien ; Beney, Laurent ; Hoskin, Roberta Targino ; da Silva Pedrini, Márcia Regina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-4fffb3db52d7f030e00eebd18e4d42cbb8310452cb24dcabb70b063d377715563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Bioavailability</topic><topic>Biochemistry, Molecular Biology</topic><topic>Bioflavonoids</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnological Products and Process Engineering</topic><topic>Biotechnology</topic><topic>Calorimetry</topic><topic>Cell viability</topic><topic>Differential scanning calorimetry</topic><topic>Diffraction</topic><topic>Digestion</topic><topic>Digestive system</topic><topic>Electronic equipment and supplies</topic><topic>Encapsulation</topic><topic>Flavones</topic><topic>Flavonoids</topic><topic>Flavonols</topic><topic>Fourier analysis</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Fourier transforms</topic><topic>Gastrointestinal tract</topic><topic>Identification and classification</topic><topic>Infrared analysis</topic><topic>Lactobacilli</topic><topic>Lactobacillus</topic><topic>Lactobacillus acidophilus</topic><topic>Life Sciences</topic><topic>Lipophilic</topic><topic>Melting point</topic><topic>Melting points</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Osmosis</topic><topic>Osmotic pressure</topic><topic>Performance evaluation</topic><topic>Plastic embedment</topic><topic>Pressure effects</topic><topic>Probiotics</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>X-ray 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biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Andrade, Eduardo Wagner Vasconcelos</au><au>Dupont, Sebastien</au><au>Beney, Laurent</au><au>Hoskin, Roberta Targino</au><au>da Silva Pedrini, Márcia Regina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osmoporation is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2022-02-01</date><risdate>2022</risdate><volume>106</volume><issue>3</issue><spage>1031</spage><epage>1044</epage><pages>1031-1044</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>The objective of this study was to evaluate the performance of
Lactobacillus acidophilus
cells as a novel encapsulating carrier for fisetin via osmoporation. Initially, the effects of osmotic pressure and initial fisetin concentration on the performance of the osmoporation process were evaluated. The best results were achieved when 15 MPa was applied, while the maximum loading capacity was reached when fisetin concentration of 2.0 mg·mL
−1
was used. For these conditions, the cell viability, encapsulation efficiency (EE), and encapsulated fisetin content (EF) were 72%, 28%, and 0.990 mg, respectively. Further, the encapsulation was confirmed by Fourier transform-infrared (FT-IR), differential scanning calorimetry (DSC), and X-ray diffraction (XRD) analysis. DSC thermograms revealed an increase of 40 °C in the melting point of fisetin after encapsulation. In addition, the enhancement of fisetin bioaccessibility by osmoporated biocapsules is shown for the first time in the literature. When the fisetin biocapsules were subjected to in vitro gastrointestinal digestion, 99.6% of the encapsulated content were retained through the gastric stage and 45.5% were released during the intestinal stage, despite no active cells were detected during simulated digestion. These results suggest that alive cells are required for an effective osmoporation-assisted encapsulation process; however, osmoporated biocapsules can efficiently protect and preserve labile compounds, independently of their activity. Overall, this study demonstrated that osmoporation using probiotic
L. acidophilus
is a simple, versatile, and efficient technique to encapsulate and deliver lipophilic fisetin for food applications.
Key points
•Fisetin is efficiently encapsulated into L. acidophilus via osmoporation.
•Fisetin bioaccessibility is improved by osmoporation into L. acidophilus.
•Release mechanisms of osmoporation carriers are independent of the cell activity.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>35024920</pmid><doi>10.1007/s00253-021-11735-8</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-3737-3500</orcidid><orcidid>https://orcid.org/0000-0003-0900-4983</orcidid><orcidid>https://orcid.org/0000-0001-6293-9636</orcidid><orcidid>https://orcid.org/0000-0002-2757-2506</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2022-02, Vol.106 (3), p.1031-1044 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03559567v1 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Analysis Bioavailability Biochemistry, Molecular Biology Bioflavonoids Biomedical and Life Sciences Biotechnological Products and Process Engineering Biotechnology Calorimetry Cell viability Differential scanning calorimetry Diffraction Digestion Digestive system Electronic equipment and supplies Encapsulation Flavones Flavonoids Flavonols Fourier analysis Fourier transform infrared spectroscopy Fourier transforms Gastrointestinal tract Identification and classification Infrared analysis Lactobacilli Lactobacillus Lactobacillus acidophilus Life Sciences Lipophilic Melting point Melting points Microbial Genetics and Genomics Microbiology Osmosis Osmotic pressure Performance evaluation Plastic embedment Pressure effects Probiotics Spectroscopy, Fourier Transform Infrared X-ray diffraction X-rays |
| title | Osmoporation is a versatile technique to encapsulate fisetin using the probiotic bacteria Lactobacillus acidophilus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T16%3A45%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Osmoporation%20is%20a%20versatile%20technique%20to%20encapsulate%20fisetin%20using%20the%20probiotic%20bacteria%20Lactobacillus%20acidophilus&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=de%20Andrade,%20Eduardo%20Wagner%20Vasconcelos&rft.date=2022-02-01&rft.volume=106&rft.issue=3&rft.spage=1031&rft.epage=1044&rft.pages=1031-1044&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-021-11735-8&rft_dat=%3Cgale_hal_p%3EA691982552%3C/gale_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2625411527&rft_id=info:pmid/35024920&rft_galeid=A691982552&rfr_iscdi=true |