Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation
Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and...
Gespeichert in:
| Veröffentlicht in: | Development (Cambridge) 2004-10, Vol.131 (20), p.5127-5138 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5138 |
|---|---|
| container_issue | 20 |
| container_start_page | 5127 |
| container_title | Development (Cambridge) |
| container_volume | 131 |
| creator | Aigouy, Benoît Van de Bor, Véronique Boeglin, Marcel Giangrande, Angela |
| description | Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and mitotic apparatus rearrangements during division and migration. Single cell labelling identifies different glia: pioneers with high filopodial, exploratory, activity and, less active followers. In combination with time-lapse, altering this cellular environment by genetic means or cell ablation has allowed to us define the role of specific cell-cell interactions. First, neurone-glia interactions are not necessary for glia motility but do affect the direction of migration. Second, repulsive interactions between glia control the extent of movement. Finally, autonomous cues control proliferation. |
| doi_str_mv | 10.1242/dev.01398 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_03447948v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66928863</sourcerecordid><originalsourceid>FETCH-LOGICAL-c486t-98e82e47ccbe790dcec362fef3cfb88cf8e74baff7bdc4f4f053447d3d1129f63</originalsourceid><addsrcrecordid>eNqFkUFr3DAQhUVpaTZJD_0DRadCDt5qLNmSjiEkTWGhl_QsZHm0qyKvtpJ3S_597XhpjjkNM3zzZh6PkM_A1lCL-luPpzUDrtU7sgIhZaWh1u_JiumGVaA1XJDLUn4zxngr5UdyAY1oNLBmRcJTGLCK9lCQ2n1PHcZIbRftGNKeZjyhjXTcIc0pIk1-AcJ-xGzdzJSpoT4-020Mlg5hm5fVWewwLQWPy-SafPA2Fvx0rlfk18P9091jtfn5_cfd7aZyQrVjpRWqGoV0rkOpWe_Q8bb26LnznVLOK5Sis97LrnfCC88aLoTseQ-Tad_yK3Kz6O5sNIccBpufTbLBPN5uzDxjM6-FOsHEfl3Y6dM_RyyjGUKZHdo9pmMxbatrpVr-JghSghAAr9ddTqVk9P9fAGbmsMwUlnkJa2K_nEWP3YD9K3lOZwLWZythu_sbMpoupJi2oYxl1sGYDgY4mJqZBmrJ_wHHXqDP</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17714411</pqid></control><display><type>article</type><title>Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><source>Company of Biologists</source><creator>Aigouy, Benoît ; Van de Bor, Véronique ; Boeglin, Marcel ; Giangrande, Angela</creator><creatorcontrib>Aigouy, Benoît ; Van de Bor, Véronique ; Boeglin, Marcel ; Giangrande, Angela</creatorcontrib><description>Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and mitotic apparatus rearrangements during division and migration. Single cell labelling identifies different glia: pioneers with high filopodial, exploratory, activity and, less active followers. In combination with time-lapse, altering this cellular environment by genetic means or cell ablation has allowed to us define the role of specific cell-cell interactions. First, neurone-glia interactions are not necessary for glia motility but do affect the direction of migration. Second, repulsive interactions between glia control the extent of movement. Finally, autonomous cues control proliferation.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.01398</identifier><identifier>PMID: 15459105</identifier><language>eng</language><publisher>England: The Company of Biologists Limited</publisher><subject>Animals ; Cell Communication - physiology ; Cell Division - physiology ; Cell Movement - physiology ; Cytoskeleton - physiology ; Diptera ; Drosophila - growth & development ; Drosophila - physiology ; Life Sciences ; Neuroglia - cytology ; Neuroglia - physiology ; Pupa - growth & development ; Pupa - physiology ; Time Factors ; Wings, Animal - growth & development ; Wings, Animal - physiology</subject><ispartof>Development (Cambridge), 2004-10, Vol.131 (20), p.5127-5138</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-98e82e47ccbe790dcec362fef3cfb88cf8e74baff7bdc4f4f053447d3d1129f63</citedby><cites>FETCH-LOGICAL-c486t-98e82e47ccbe790dcec362fef3cfb88cf8e74baff7bdc4f4f053447d3d1129f63</cites><orcidid>0000-0001-6278-5120</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,3665,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15459105$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03447948$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Aigouy, Benoît</creatorcontrib><creatorcontrib>Van de Bor, Véronique</creatorcontrib><creatorcontrib>Boeglin, Marcel</creatorcontrib><creatorcontrib>Giangrande, Angela</creatorcontrib><title>Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and mitotic apparatus rearrangements during division and migration. Single cell labelling identifies different glia: pioneers with high filopodial, exploratory, activity and, less active followers. In combination with time-lapse, altering this cellular environment by genetic means or cell ablation has allowed to us define the role of specific cell-cell interactions. First, neurone-glia interactions are not necessary for glia motility but do affect the direction of migration. Second, repulsive interactions between glia control the extent of movement. Finally, autonomous cues control proliferation.</description><subject>Animals</subject><subject>Cell Communication - physiology</subject><subject>Cell Division - physiology</subject><subject>Cell Movement - physiology</subject><subject>Cytoskeleton - physiology</subject><subject>Diptera</subject><subject>Drosophila - growth & development</subject><subject>Drosophila - physiology</subject><subject>Life Sciences</subject><subject>Neuroglia - cytology</subject><subject>Neuroglia - physiology</subject><subject>Pupa - growth & development</subject><subject>Pupa - physiology</subject><subject>Time Factors</subject><subject>Wings, Animal - growth & development</subject><subject>Wings, Animal - physiology</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFr3DAQhUVpaTZJD_0DRadCDt5qLNmSjiEkTWGhl_QsZHm0qyKvtpJ3S_597XhpjjkNM3zzZh6PkM_A1lCL-luPpzUDrtU7sgIhZaWh1u_JiumGVaA1XJDLUn4zxngr5UdyAY1oNLBmRcJTGLCK9lCQ2n1PHcZIbRftGNKeZjyhjXTcIc0pIk1-AcJ-xGzdzJSpoT4-020Mlg5hm5fVWewwLQWPy-SafPA2Fvx0rlfk18P9091jtfn5_cfd7aZyQrVjpRWqGoV0rkOpWe_Q8bb26LnznVLOK5Sis97LrnfCC88aLoTseQ-Tad_yK3Kz6O5sNIccBpufTbLBPN5uzDxjM6-FOsHEfl3Y6dM_RyyjGUKZHdo9pmMxbatrpVr-JghSghAAr9ddTqVk9P9fAGbmsMwUlnkJa2K_nEWP3YD9K3lOZwLWZythu_sbMpoupJi2oYxl1sGYDgY4mJqZBmrJ_wHHXqDP</recordid><startdate>20041015</startdate><enddate>20041015</enddate><creator>Aigouy, Benoît</creator><creator>Van de Bor, Véronique</creator><creator>Boeglin, Marcel</creator><creator>Giangrande, Angela</creator><general>The Company of Biologists Limited</general><general>Company of Biologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-6278-5120</orcidid></search><sort><creationdate>20041015</creationdate><title>Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation</title><author>Aigouy, Benoît ; Van de Bor, Véronique ; Boeglin, Marcel ; Giangrande, Angela</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-98e82e47ccbe790dcec362fef3cfb88cf8e74baff7bdc4f4f053447d3d1129f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Cell Communication - physiology</topic><topic>Cell Division - physiology</topic><topic>Cell Movement - physiology</topic><topic>Cytoskeleton - physiology</topic><topic>Diptera</topic><topic>Drosophila - growth & development</topic><topic>Drosophila - physiology</topic><topic>Life Sciences</topic><topic>Neuroglia - cytology</topic><topic>Neuroglia - physiology</topic><topic>Pupa - growth & development</topic><topic>Pupa - physiology</topic><topic>Time Factors</topic><topic>Wings, Animal - growth & development</topic><topic>Wings, Animal - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aigouy, Benoît</creatorcontrib><creatorcontrib>Van de Bor, Véronique</creatorcontrib><creatorcontrib>Boeglin, Marcel</creatorcontrib><creatorcontrib>Giangrande, Angela</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aigouy, Benoît</au><au>Van de Bor, Véronique</au><au>Boeglin, Marcel</au><au>Giangrande, Angela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>2004-10-15</date><risdate>2004</risdate><volume>131</volume><issue>20</issue><spage>5127</spage><epage>5138</epage><pages>5127-5138</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and mitotic apparatus rearrangements during division and migration. Single cell labelling identifies different glia: pioneers with high filopodial, exploratory, activity and, less active followers. In combination with time-lapse, altering this cellular environment by genetic means or cell ablation has allowed to us define the role of specific cell-cell interactions. First, neurone-glia interactions are not necessary for glia motility but do affect the direction of migration. Second, repulsive interactions between glia control the extent of movement. Finally, autonomous cues control proliferation.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>15459105</pmid><doi>10.1242/dev.01398</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-6278-5120</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 2004-10, Vol.131 (20), p.5127-5138 |
| issn | 0950-1991 1477-9129 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03447948v1 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Cell Communication - physiology Cell Division - physiology Cell Movement - physiology Cytoskeleton - physiology Diptera Drosophila - growth & development Drosophila - physiology Life Sciences Neuroglia - cytology Neuroglia - physiology Pupa - growth & development Pupa - physiology Time Factors Wings, Animal - growth & development Wings, Animal - physiology |
| title | Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T06%3A03%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Time-lapse%20and%20cell%20ablation%20reveal%20the%20role%20of%20cell%20interactions%20in%20fly%20glia%20migration%20and%20proliferation&rft.jtitle=Development%20(Cambridge)&rft.au=Aigouy,%20Beno%C3%AEt&rft.date=2004-10-15&rft.volume=131&rft.issue=20&rft.spage=5127&rft.epage=5138&rft.pages=5127-5138&rft.issn=0950-1991&rft.eissn=1477-9129&rft_id=info:doi/10.1242/dev.01398&rft_dat=%3Cproquest_hal_p%3E66928863%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17714411&rft_id=info:pmid/15459105&rfr_iscdi=true |