Physical training and hypertension have opposite effects on endothelial brain-derived neurotrophic factor expression
Changes in circulating brain-derived neurotrophic factor (BDNF) levels were reported in patients with or at risk for cardiovascular diseases associated with endothelial dysfunction, suggesting a link between BDNF and endothelial functionality. However, little is known on cardiovascular BDNF. Our aim...
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| Veröffentlicht in: | Cardiovascular research 2013-12, Vol.100 (3), p.374-382 |
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| container_title | Cardiovascular research |
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| creator | Prigent-Tessier, Anne Quirié, Aurore Maguin-Gaté, Katy Szostak, Justyna Mossiat, Claude Nappey, Maude Devaux, Sylvie Marie, Christine Demougeot, Céline |
| description | Changes in circulating brain-derived neurotrophic factor (BDNF) levels were reported in patients with or at risk for cardiovascular diseases associated with endothelial dysfunction, suggesting a link between BDNF and endothelial functionality. However, little is known on cardiovascular BDNF. Our aim was to investigate levels/localization, function, and relevance of cardiovascular BDNF.
BDNF levels (western blotting) and localization (immunostaining) were assessed in the heart and aorta from rats with impaired (spontaneously hypertensive rats [SHR]), normal (Wistar Kyoto rats [WKY]), and improved (SHR and WKY subjected to physical training) endothelial function. BDNF levels were also measured in cultured endothelial cells (CECs) subjected to low and high shear stress. The cardiovascular effects of BDNF were investigated in isolated aortic rings and hearts. The results showed high BDNF levels in the heart and aorta, the expression being prominent in endothelial cells as compared with other cell types. Exogenous BDNF vasodilated aortic rings but changed neither coronary flow nor cardiac contractility. Hypertension was associated with decreased expression of BDNF in the endothelium, whereas physical training led to endothelial BDNF up-regulation not only in WKY but also in SHR. Exposure of CECs to high shear stress stimulated BDNF production and secretion.
Cardiovascular BDNF is mainly localized within endothelial cells in which its expression is dependent on endothelial function. These results open new perspectives on the role of endothelial BDNF in cardiovascular health. |
| doi_str_mv | 10.1093/cvr/cvt219 |
| format | Article |
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BDNF levels (western blotting) and localization (immunostaining) were assessed in the heart and aorta from rats with impaired (spontaneously hypertensive rats [SHR]), normal (Wistar Kyoto rats [WKY]), and improved (SHR and WKY subjected to physical training) endothelial function. BDNF levels were also measured in cultured endothelial cells (CECs) subjected to low and high shear stress. The cardiovascular effects of BDNF were investigated in isolated aortic rings and hearts. The results showed high BDNF levels in the heart and aorta, the expression being prominent in endothelial cells as compared with other cell types. Exogenous BDNF vasodilated aortic rings but changed neither coronary flow nor cardiac contractility. Hypertension was associated with decreased expression of BDNF in the endothelium, whereas physical training led to endothelial BDNF up-regulation not only in WKY but also in SHR. Exposure of CECs to high shear stress stimulated BDNF production and secretion.
Cardiovascular BDNF is mainly localized within endothelial cells in which its expression is dependent on endothelial function. These results open new perspectives on the role of endothelial BDNF in cardiovascular health.</description><identifier>ISSN: 0008-6363</identifier><identifier>EISSN: 1755-3245</identifier><identifier>DOI: 10.1093/cvr/cvt219</identifier><identifier>PMID: 24092446</identifier><language>eng</language><publisher>England: Oxford University Press (OUP)</publisher><subject>Animal biology ; Animals ; Aorta, Thoracic - metabolism ; Aorta, Thoracic - physiopathology ; Brain-Derived Neurotrophic Factor - metabolism ; Cells, Cultured ; Coronary Circulation ; Coronary Vessels - metabolism ; Coronary Vessels - physiopathology ; Disease Models, Animal ; Endothelial Cells - metabolism ; Hypertension - metabolism ; Hypertension - physiopathology ; Life Sciences ; Male ; Myocardial Contraction ; Physical Conditioning, Animal ; Rats ; Rats, Inbred SHR ; Rats, Inbred WKY ; Regional Blood Flow ; Stress, Mechanical ; Time Factors ; Vasodilation ; Ventricular Function, Left ; Ventricular Pressure</subject><ispartof>Cardiovascular research, 2013-12, Vol.100 (3), p.374-382</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-d9d30dbc32cdab45950ba97314566b2f86b39d1433d150f76795a1e7cff1b3033</citedby><cites>FETCH-LOGICAL-c464t-d9d30dbc32cdab45950ba97314566b2f86b39d1433d150f76795a1e7cff1b3033</cites><orcidid>0000-0003-1894-7908 ; 0000-0002-8926-1834</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24092446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03408736$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Prigent-Tessier, Anne</creatorcontrib><creatorcontrib>Quirié, Aurore</creatorcontrib><creatorcontrib>Maguin-Gaté, Katy</creatorcontrib><creatorcontrib>Szostak, Justyna</creatorcontrib><creatorcontrib>Mossiat, Claude</creatorcontrib><creatorcontrib>Nappey, Maude</creatorcontrib><creatorcontrib>Devaux, Sylvie</creatorcontrib><creatorcontrib>Marie, Christine</creatorcontrib><creatorcontrib>Demougeot, Céline</creatorcontrib><title>Physical training and hypertension have opposite effects on endothelial brain-derived neurotrophic factor expression</title><title>Cardiovascular research</title><addtitle>Cardiovasc Res</addtitle><description>Changes in circulating brain-derived neurotrophic factor (BDNF) levels were reported in patients with or at risk for cardiovascular diseases associated with endothelial dysfunction, suggesting a link between BDNF and endothelial functionality. However, little is known on cardiovascular BDNF. Our aim was to investigate levels/localization, function, and relevance of cardiovascular BDNF.
BDNF levels (western blotting) and localization (immunostaining) were assessed in the heart and aorta from rats with impaired (spontaneously hypertensive rats [SHR]), normal (Wistar Kyoto rats [WKY]), and improved (SHR and WKY subjected to physical training) endothelial function. BDNF levels were also measured in cultured endothelial cells (CECs) subjected to low and high shear stress. The cardiovascular effects of BDNF were investigated in isolated aortic rings and hearts. The results showed high BDNF levels in the heart and aorta, the expression being prominent in endothelial cells as compared with other cell types. Exogenous BDNF vasodilated aortic rings but changed neither coronary flow nor cardiac contractility. Hypertension was associated with decreased expression of BDNF in the endothelium, whereas physical training led to endothelial BDNF up-regulation not only in WKY but also in SHR. Exposure of CECs to high shear stress stimulated BDNF production and secretion.
Cardiovascular BDNF is mainly localized within endothelial cells in which its expression is dependent on endothelial function. These results open new perspectives on the role of endothelial BDNF in cardiovascular health.</description><subject>Animal biology</subject><subject>Animals</subject><subject>Aorta, Thoracic - metabolism</subject><subject>Aorta, Thoracic - physiopathology</subject><subject>Brain-Derived Neurotrophic Factor - metabolism</subject><subject>Cells, Cultured</subject><subject>Coronary Circulation</subject><subject>Coronary Vessels - metabolism</subject><subject>Coronary Vessels - physiopathology</subject><subject>Disease Models, Animal</subject><subject>Endothelial Cells - metabolism</subject><subject>Hypertension - metabolism</subject><subject>Hypertension - physiopathology</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Myocardial Contraction</subject><subject>Physical Conditioning, Animal</subject><subject>Rats</subject><subject>Rats, Inbred SHR</subject><subject>Rats, Inbred WKY</subject><subject>Regional Blood Flow</subject><subject>Stress, Mechanical</subject><subject>Time Factors</subject><subject>Vasodilation</subject><subject>Ventricular Function, Left</subject><subject>Ventricular Pressure</subject><issn>0008-6363</issn><issn>1755-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU9PGzEQxa0KBCHlwgeofIRKS-31n42PCJWmUqRyoGfLa4-7Rpv11nai5tvXUYDDaDQzv_fm8BC6oeSeEsW-2X2qVVqqPqEF7YRoWMvFGVoQQlaNZJJdoqucX-soRMcv0GXLiWo5lwtUnodDDtaMuCQTpjD9wWZyeDjMkApMOcQJD2YPOM5zzKEABu_BlozrASYXywBjqPL-KG8cpLAHhyfYpVhSnIdgsTe2xITh35wgHx0_o3NvxgzXb32Jfj99f3lcN5tfP34-PmwayyUvjVOOEddb1lpnei6UIL1RHaNcSNm3fiV7phzljDkqiO9kp4Sh0Fnvac8IY0t0d_IdzKjnFLYmHXQ0Qa8fNvq4I4yTVcfknlb29sTOKf7dQS56G7KFcTQTxF3W9amiQq6orOjXE2pTzDmB__CmRB8T0TURfUqkwl_efHf9FtwH-h4B-w_yt4nJ</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Prigent-Tessier, Anne</creator><creator>Quirié, Aurore</creator><creator>Maguin-Gaté, Katy</creator><creator>Szostak, Justyna</creator><creator>Mossiat, Claude</creator><creator>Nappey, Maude</creator><creator>Devaux, Sylvie</creator><creator>Marie, Christine</creator><creator>Demougeot, Céline</creator><general>Oxford University Press (OUP)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-1894-7908</orcidid><orcidid>https://orcid.org/0000-0002-8926-1834</orcidid></search><sort><creationdate>20131201</creationdate><title>Physical training and hypertension have opposite effects on endothelial brain-derived neurotrophic factor expression</title><author>Prigent-Tessier, Anne ; Quirié, Aurore ; Maguin-Gaté, Katy ; Szostak, Justyna ; Mossiat, Claude ; Nappey, Maude ; Devaux, Sylvie ; Marie, Christine ; Demougeot, Céline</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-d9d30dbc32cdab45950ba97314566b2f86b39d1433d150f76795a1e7cff1b3033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal biology</topic><topic>Animals</topic><topic>Aorta, Thoracic - metabolism</topic><topic>Aorta, Thoracic - physiopathology</topic><topic>Brain-Derived Neurotrophic Factor - metabolism</topic><topic>Cells, Cultured</topic><topic>Coronary Circulation</topic><topic>Coronary Vessels - metabolism</topic><topic>Coronary Vessels - physiopathology</topic><topic>Disease Models, Animal</topic><topic>Endothelial Cells - metabolism</topic><topic>Hypertension - metabolism</topic><topic>Hypertension - physiopathology</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Myocardial Contraction</topic><topic>Physical Conditioning, Animal</topic><topic>Rats</topic><topic>Rats, Inbred SHR</topic><topic>Rats, Inbred WKY</topic><topic>Regional Blood Flow</topic><topic>Stress, Mechanical</topic><topic>Time Factors</topic><topic>Vasodilation</topic><topic>Ventricular Function, Left</topic><topic>Ventricular Pressure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prigent-Tessier, Anne</creatorcontrib><creatorcontrib>Quirié, Aurore</creatorcontrib><creatorcontrib>Maguin-Gaté, Katy</creatorcontrib><creatorcontrib>Szostak, Justyna</creatorcontrib><creatorcontrib>Mossiat, Claude</creatorcontrib><creatorcontrib>Nappey, Maude</creatorcontrib><creatorcontrib>Devaux, Sylvie</creatorcontrib><creatorcontrib>Marie, Christine</creatorcontrib><creatorcontrib>Demougeot, Céline</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Cardiovascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prigent-Tessier, Anne</au><au>Quirié, Aurore</au><au>Maguin-Gaté, Katy</au><au>Szostak, Justyna</au><au>Mossiat, Claude</au><au>Nappey, Maude</au><au>Devaux, Sylvie</au><au>Marie, Christine</au><au>Demougeot, Céline</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physical training and hypertension have opposite effects on endothelial brain-derived neurotrophic factor expression</atitle><jtitle>Cardiovascular research</jtitle><addtitle>Cardiovasc Res</addtitle><date>2013-12-01</date><risdate>2013</risdate><volume>100</volume><issue>3</issue><spage>374</spage><epage>382</epage><pages>374-382</pages><issn>0008-6363</issn><eissn>1755-3245</eissn><abstract>Changes in circulating brain-derived neurotrophic factor (BDNF) levels were reported in patients with or at risk for cardiovascular diseases associated with endothelial dysfunction, suggesting a link between BDNF and endothelial functionality. However, little is known on cardiovascular BDNF. Our aim was to investigate levels/localization, function, and relevance of cardiovascular BDNF.
BDNF levels (western blotting) and localization (immunostaining) were assessed in the heart and aorta from rats with impaired (spontaneously hypertensive rats [SHR]), normal (Wistar Kyoto rats [WKY]), and improved (SHR and WKY subjected to physical training) endothelial function. BDNF levels were also measured in cultured endothelial cells (CECs) subjected to low and high shear stress. The cardiovascular effects of BDNF were investigated in isolated aortic rings and hearts. The results showed high BDNF levels in the heart and aorta, the expression being prominent in endothelial cells as compared with other cell types. Exogenous BDNF vasodilated aortic rings but changed neither coronary flow nor cardiac contractility. Hypertension was associated with decreased expression of BDNF in the endothelium, whereas physical training led to endothelial BDNF up-regulation not only in WKY but also in SHR. Exposure of CECs to high shear stress stimulated BDNF production and secretion.
Cardiovascular BDNF is mainly localized within endothelial cells in which its expression is dependent on endothelial function. These results open new perspectives on the role of endothelial BDNF in cardiovascular health.</abstract><cop>England</cop><pub>Oxford University Press (OUP)</pub><pmid>24092446</pmid><doi>10.1093/cvr/cvt219</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-1894-7908</orcidid><orcidid>https://orcid.org/0000-0002-8926-1834</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0008-6363 |
| ispartof | Cardiovascular research, 2013-12, Vol.100 (3), p.374-382 |
| issn | 0008-6363 1755-3245 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animal biology Animals Aorta, Thoracic - metabolism Aorta, Thoracic - physiopathology Brain-Derived Neurotrophic Factor - metabolism Cells, Cultured Coronary Circulation Coronary Vessels - metabolism Coronary Vessels - physiopathology Disease Models, Animal Endothelial Cells - metabolism Hypertension - metabolism Hypertension - physiopathology Life Sciences Male Myocardial Contraction Physical Conditioning, Animal Rats Rats, Inbred SHR Rats, Inbred WKY Regional Blood Flow Stress, Mechanical Time Factors Vasodilation Ventricular Function, Left Ventricular Pressure |
| title | Physical training and hypertension have opposite effects on endothelial brain-derived neurotrophic factor expression |
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