Electrochemical Trimethylamine N -Oxide Biosensor with Enzyme-Based Oxygen-Scavenging Membrane for Long-Term Operation under Ambient Air
An amperometric trimethylamine -oxide (TMAO) biosensor is reported, where TMAO reductase (TorA) and glucose oxidase (GOD) and catalase (Cat) were immobilized on the electrode surface, enabling measurements of mediated enzymatic TMAO reduction at low potential under ambient air conditions. The oxygen...
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| Veröffentlicht in: | Biosensors (Basel) 2021-03, Vol.11 (4), p.98 |
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| creator | Waffo, Armel F T Mitrova, Biljana Tiedemann, Kim Iobbi-Nivol, Chantal Leimkühler, Silke Wollenberger, Ulla |
| description | An amperometric trimethylamine
-oxide (TMAO) biosensor is reported, where TMAO reductase (TorA) and glucose oxidase (GOD) and catalase (Cat) were immobilized on the electrode surface, enabling measurements of mediated enzymatic TMAO reduction at low potential under ambient air conditions. The oxygen anti-interference membrane composed of GOD, Cat and polyvinyl alcohol (PVA) hydrogel, together with glucose concentration, was optimized until the O
reduction current of a Clark-type electrode was completely suppressed for at least 3 h. For the preparation of the TMAO biosensor,
TorA was purified under anaerobic conditions and immobilized on the surface of a carbon electrode and covered by the optimized O
scavenging membrane. The TMAO sensor operates at a potential of -0.8 V vs. Ag/AgCl (1 M KCl), where the reduction of methylviologen (MV) is recorded. The sensor signal depends linearly on TMAO concentrations between 2 µM and 15 mM, with a sensitivity of 2.75 ± 1.7 µA/mM. The developed biosensor is characterized by a response time of about 33 s and an operational stability over 3 weeks. Furthermore, measurements of TMAO concentration were performed in 10% human serum, where the lowest detectable concentration is of 10 µM TMAO. |
| doi_str_mv | 10.3390/bios11040098 |
| format | Article |
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-oxide (TMAO) biosensor is reported, where TMAO reductase (TorA) and glucose oxidase (GOD) and catalase (Cat) were immobilized on the electrode surface, enabling measurements of mediated enzymatic TMAO reduction at low potential under ambient air conditions. The oxygen anti-interference membrane composed of GOD, Cat and polyvinyl alcohol (PVA) hydrogel, together with glucose concentration, was optimized until the O
reduction current of a Clark-type electrode was completely suppressed for at least 3 h. For the preparation of the TMAO biosensor,
TorA was purified under anaerobic conditions and immobilized on the surface of a carbon electrode and covered by the optimized O
scavenging membrane. The TMAO sensor operates at a potential of -0.8 V vs. Ag/AgCl (1 M KCl), where the reduction of methylviologen (MV) is recorded. The sensor signal depends linearly on TMAO concentrations between 2 µM and 15 mM, with a sensitivity of 2.75 ± 1.7 µA/mM. The developed biosensor is characterized by a response time of about 33 s and an operational stability over 3 weeks. Furthermore, measurements of TMAO concentration were performed in 10% human serum, where the lowest detectable concentration is of 10 µM TMAO.</description><identifier>ISSN: 2079-6374</identifier><identifier>EISSN: 2079-6374</identifier><identifier>DOI: 10.3390/bios11040098</identifier><identifier>PMID: 33801724</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Anaerobic conditions ; Biosensing Techniques ; biosensor ; Biosensors ; Carbon ; Catalase ; Chromatography ; E coli ; Electrical measurement ; Electrochemistry ; Electrodes ; Enzymes ; Escherichia coli ; Glucose ; Glucose Oxidase ; Hemodialysis ; Human performance ; Humans ; Hydrogels ; immobilized enzyme ; Laboratories ; Life Sciences ; Mass spectrometry ; Membranes ; Metabolism ; Methylamines - analysis ; Microbiota ; Molybdenum ; multienzyme electrode ; Oxygen ; oxygen scavenger ; Polyvinyl alcohol ; Potassium chloride ; Reductases ; Reduction ; Response time ; Scavenging ; Scientific imaging ; Silver chloride ; TMAO-reductase ; Trimethylamine ; trimethylamine N-oxide</subject><ispartof>Biosensors (Basel), 2021-03, Vol.11 (4), p.98</ispartof><rights>2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Attribution</rights><rights>2021 by the authors. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-be95b8a98ad8fe6456dd38d4c5bdceeac595cfaf5390fbf8331913dfe6eff2a13</citedby><cites>FETCH-LOGICAL-c512t-be95b8a98ad8fe6456dd38d4c5bdceeac595cfaf5390fbf8331913dfe6eff2a13</cites><orcidid>0000-0002-7088-8928 ; 0000-0003-4021-1438 ; 0000-0002-2462-3283</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066520/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8066520/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33801724$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03367470$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Waffo, Armel F T</creatorcontrib><creatorcontrib>Mitrova, Biljana</creatorcontrib><creatorcontrib>Tiedemann, Kim</creatorcontrib><creatorcontrib>Iobbi-Nivol, Chantal</creatorcontrib><creatorcontrib>Leimkühler, Silke</creatorcontrib><creatorcontrib>Wollenberger, Ulla</creatorcontrib><title>Electrochemical Trimethylamine N -Oxide Biosensor with Enzyme-Based Oxygen-Scavenging Membrane for Long-Term Operation under Ambient Air</title><title>Biosensors (Basel)</title><addtitle>Biosensors (Basel)</addtitle><description>An amperometric trimethylamine
-oxide (TMAO) biosensor is reported, where TMAO reductase (TorA) and glucose oxidase (GOD) and catalase (Cat) were immobilized on the electrode surface, enabling measurements of mediated enzymatic TMAO reduction at low potential under ambient air conditions. The oxygen anti-interference membrane composed of GOD, Cat and polyvinyl alcohol (PVA) hydrogel, together with glucose concentration, was optimized until the O
reduction current of a Clark-type electrode was completely suppressed for at least 3 h. For the preparation of the TMAO biosensor,
TorA was purified under anaerobic conditions and immobilized on the surface of a carbon electrode and covered by the optimized O
scavenging membrane. The TMAO sensor operates at a potential of -0.8 V vs. Ag/AgCl (1 M KCl), where the reduction of methylviologen (MV) is recorded. The sensor signal depends linearly on TMAO concentrations between 2 µM and 15 mM, with a sensitivity of 2.75 ± 1.7 µA/mM. The developed biosensor is characterized by a response time of about 33 s and an operational stability over 3 weeks. Furthermore, measurements of TMAO concentration were performed in 10% human serum, where the lowest detectable concentration is of 10 µM TMAO.</description><subject>Anaerobic conditions</subject><subject>Biosensing Techniques</subject><subject>biosensor</subject><subject>Biosensors</subject><subject>Carbon</subject><subject>Catalase</subject><subject>Chromatography</subject><subject>E coli</subject><subject>Electrical measurement</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Glucose</subject><subject>Glucose Oxidase</subject><subject>Hemodialysis</subject><subject>Human performance</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>immobilized enzyme</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Membranes</subject><subject>Metabolism</subject><subject>Methylamines - 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Silke</au><au>Wollenberger, Ulla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical Trimethylamine N -Oxide Biosensor with Enzyme-Based Oxygen-Scavenging Membrane for Long-Term Operation under Ambient Air</atitle><jtitle>Biosensors (Basel)</jtitle><addtitle>Biosensors (Basel)</addtitle><date>2021-03-27</date><risdate>2021</risdate><volume>11</volume><issue>4</issue><spage>98</spage><pages>98-</pages><issn>2079-6374</issn><eissn>2079-6374</eissn><abstract>An amperometric trimethylamine
-oxide (TMAO) biosensor is reported, where TMAO reductase (TorA) and glucose oxidase (GOD) and catalase (Cat) were immobilized on the electrode surface, enabling measurements of mediated enzymatic TMAO reduction at low potential under ambient air conditions. The oxygen anti-interference membrane composed of GOD, Cat and polyvinyl alcohol (PVA) hydrogel, together with glucose concentration, was optimized until the O
reduction current of a Clark-type electrode was completely suppressed for at least 3 h. For the preparation of the TMAO biosensor,
TorA was purified under anaerobic conditions and immobilized on the surface of a carbon electrode and covered by the optimized O
scavenging membrane. The TMAO sensor operates at a potential of -0.8 V vs. Ag/AgCl (1 M KCl), where the reduction of methylviologen (MV) is recorded. The sensor signal depends linearly on TMAO concentrations between 2 µM and 15 mM, with a sensitivity of 2.75 ± 1.7 µA/mM. The developed biosensor is characterized by a response time of about 33 s and an operational stability over 3 weeks. Furthermore, measurements of TMAO concentration were performed in 10% human serum, where the lowest detectable concentration is of 10 µM TMAO.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>33801724</pmid><doi>10.3390/bios11040098</doi><orcidid>https://orcid.org/0000-0002-7088-8928</orcidid><orcidid>https://orcid.org/0000-0003-4021-1438</orcidid><orcidid>https://orcid.org/0000-0002-2462-3283</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; MDPI - Multidisciplinary Digital Publishing Institute; PubMed Central |
| subjects | Anaerobic conditions Biosensing Techniques biosensor Biosensors Carbon Catalase Chromatography E coli Electrical measurement Electrochemistry Electrodes Enzymes Escherichia coli Glucose Glucose Oxidase Hemodialysis Human performance Humans Hydrogels immobilized enzyme Laboratories Life Sciences Mass spectrometry Membranes Metabolism Methylamines - analysis Microbiota Molybdenum multienzyme electrode Oxygen oxygen scavenger Polyvinyl alcohol Potassium chloride Reductases Reduction Response time Scavenging Scientific imaging Silver chloride TMAO-reductase Trimethylamine trimethylamine N-oxide |
| title | Electrochemical Trimethylamine N -Oxide Biosensor with Enzyme-Based Oxygen-Scavenging Membrane for Long-Term Operation under Ambient Air |
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