Localization of Free and Bound Biotin in Cells from Green Pea Leaves

Cytosol and vacuoles from protoplasts, chloroplasts, and mitochondria from green pea ( Pisum sativum) leaves were purified and examined for their biotin content. The bulk of free biotin was shown to be exclusively associated with the cytosolic fraction at a concentration of about 4 pmol/mg protein a...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1993-05, Vol.303 (1), p.67-73
Hauptverfasser:	Baldet, P., Alban, C., Axiotis, S., Douce, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	bioassays Biological and medical sciences biosintesis biosynthese biosynthesis biotin Biotin - chemistry biotina biotine Carboxylic Ester Hydrolases - metabolism Cell biochemistry Cell physiology chloroplaste chloroplasts Chloroplasts - chemistry cloroplasto dosage biologique ensayo biologico escherichia Fabaceae - chemistry feuille Fundamental and applied biological sciences. Psychology hojas leaves Life Sciences mitochondria Mitochondria - chemistry mitochondrie mitocondria pisum sativum Plant physiology and development Plant Proteins - chemistry Plants, Medicinal protoplaste protoplastos protoplasts Solubility Subcellular Fractions - chemistry Vacuoles - chemistry Vegetal Biology
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