AtDTX25, a member of the multidrug and toxic compound extrusion family, is a vacuolar ascorbate transporter that controls intracellular iron cycling in Arabidopsis
Summary Iron (Fe) is an essential element, its transport is regulated by the cell redox balance. In seeds, Fe enters the embryo as Fe2+ and is stored in vacuoles as Fe3+. Through its ferric reduction activity, ascorbate plays a major role in Fe redox state and therefore Fe transport within the seed....
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| Veröffentlicht in: | The New phytologist 2021-09, Vol.231 (5), p.1956-1967 |
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| container_end_page | 1967 |
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| container_issue | 5 |
| container_start_page | 1956 |
| container_title | The New phytologist |
| container_volume | 231 |
| creator | Hoang, Minh Thi Thanh Almeida, Diego Chay, Sandrine Alcon, Carine Corratge‐Faillie, Claire Curie, Catherine Mari, Stephane |
| description | Summary
Iron (Fe) is an essential element, its transport is regulated by the cell redox balance. In seeds, Fe enters the embryo as Fe2+ and is stored in vacuoles as Fe3+. Through its ferric reduction activity, ascorbate plays a major role in Fe redox state and therefore Fe transport within the seed.
We searched for ascorbate membrane transporters responsible for controlling Fe reduction by screening the yeast ferric reductase‐deficient fre1 strain and isolated AtDTX25, a member of the Multidrug And Toxic compound Extrusion (MATE) family.
AtDTX25 was shown to mediate ascorbate efflux when expressed in yeast and Xenopus oocytes, in a pH‐dependent manner. In planta, AtDTX25 is highly expressed during germination and encodes a vacuolar membrane protein. Isolated vacuoles from AtDTX25‐1 knockout mutant contained less ascorbate and more Fe than wild‐type (WT), and mutant seedlings were highly sensitive to Fe deficiency. Iron imaging further showed that the remobilisation of Fe from vacuoles was highly impaired in mutant seedlings.
Taken together, our results established AtDTX25 as a vacuolar ascorbate transporter, required during germination to promote the reduction of the pool of stored Fe3+ and its remobilisation to feed the developing seedling. |
| doi_str_mv | 10.1111/nph.17526 |
| format | Article |
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Iron (Fe) is an essential element, its transport is regulated by the cell redox balance. In seeds, Fe enters the embryo as Fe2+ and is stored in vacuoles as Fe3+. Through its ferric reduction activity, ascorbate plays a major role in Fe redox state and therefore Fe transport within the seed.
We searched for ascorbate membrane transporters responsible for controlling Fe reduction by screening the yeast ferric reductase‐deficient fre1 strain and isolated AtDTX25, a member of the Multidrug And Toxic compound Extrusion (MATE) family.
AtDTX25 was shown to mediate ascorbate efflux when expressed in yeast and Xenopus oocytes, in a pH‐dependent manner. In planta, AtDTX25 is highly expressed during germination and encodes a vacuolar membrane protein. Isolated vacuoles from AtDTX25‐1 knockout mutant contained less ascorbate and more Fe than wild‐type (WT), and mutant seedlings were highly sensitive to Fe deficiency. Iron imaging further showed that the remobilisation of Fe from vacuoles was highly impaired in mutant seedlings.
Taken together, our results established AtDTX25 as a vacuolar ascorbate transporter, required during germination to promote the reduction of the pool of stored Fe3+ and its remobilisation to feed the developing seedling.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/nph.17526</identifier><identifier>PMID: 34080200</identifier><language>eng</language><publisher>Lancaster: Wiley Subscription Services, Inc</publisher><subject>Animal embryos ; Arabidopsis ; ascorbate ; Ascorbic acid ; Efflux ; Embryos ; Extrusion ; Gametocytes ; Germination ; Iron ; Iron deficiency ; Life Sciences ; MATE transporter ; Membrane proteins ; Membranes ; Mutants ; Nutrient deficiency ; Oocytes ; Oxidoreductions ; Redox properties ; Reductases ; Reduction ; Seedlings ; Seeds ; Transport ; vacuole ; Vacuoles ; Vegetal Biology ; Yeast ; Yeasts</subject><ispartof>The New phytologist, 2021-09, Vol.231 (5), p.1956-1967</ispartof><rights>2021 The Authors © 2021 New Phytologist Foundation</rights><rights>Copyright © 2021 New Phytologist Trust</rights><rights>Attribution</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3996-b3900d6d3e4469741ca2e864899494bb036df12fc999f34cce1ec5f7a5c7ba003</citedby><cites>FETCH-LOGICAL-c3996-b3900d6d3e4469741ca2e864899494bb036df12fc999f34cce1ec5f7a5c7ba003</cites><orcidid>0000-0001-5413-7844 ; 0000-0002-8884-1175 ; 0000-0001-7040-8253 ; 0000-0003-3296-5029 ; 0000-0002-5738-6712</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fnph.17526$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fnph.17526$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://hal.inrae.fr/hal-03258348$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoang, Minh Thi Thanh</creatorcontrib><creatorcontrib>Almeida, Diego</creatorcontrib><creatorcontrib>Chay, Sandrine</creatorcontrib><creatorcontrib>Alcon, Carine</creatorcontrib><creatorcontrib>Corratge‐Faillie, Claire</creatorcontrib><creatorcontrib>Curie, Catherine</creatorcontrib><creatorcontrib>Mari, Stephane</creatorcontrib><title>AtDTX25, a member of the multidrug and toxic compound extrusion family, is a vacuolar ascorbate transporter that controls intracellular iron cycling in Arabidopsis</title><title>The New phytologist</title><description>Summary
Iron (Fe) is an essential element, its transport is regulated by the cell redox balance. In seeds, Fe enters the embryo as Fe2+ and is stored in vacuoles as Fe3+. Through its ferric reduction activity, ascorbate plays a major role in Fe redox state and therefore Fe transport within the seed.
We searched for ascorbate membrane transporters responsible for controlling Fe reduction by screening the yeast ferric reductase‐deficient fre1 strain and isolated AtDTX25, a member of the Multidrug And Toxic compound Extrusion (MATE) family.
AtDTX25 was shown to mediate ascorbate efflux when expressed in yeast and Xenopus oocytes, in a pH‐dependent manner. In planta, AtDTX25 is highly expressed during germination and encodes a vacuolar membrane protein. Isolated vacuoles from AtDTX25‐1 knockout mutant contained less ascorbate and more Fe than wild‐type (WT), and mutant seedlings were highly sensitive to Fe deficiency. Iron imaging further showed that the remobilisation of Fe from vacuoles was highly impaired in mutant seedlings.
Taken together, our results established AtDTX25 as a vacuolar ascorbate transporter, required during germination to promote the reduction of the pool of stored Fe3+ and its remobilisation to feed the developing seedling.</description><subject>Animal embryos</subject><subject>Arabidopsis</subject><subject>ascorbate</subject><subject>Ascorbic acid</subject><subject>Efflux</subject><subject>Embryos</subject><subject>Extrusion</subject><subject>Gametocytes</subject><subject>Germination</subject><subject>Iron</subject><subject>Iron deficiency</subject><subject>Life Sciences</subject><subject>MATE transporter</subject><subject>Membrane proteins</subject><subject>Membranes</subject><subject>Mutants</subject><subject>Nutrient deficiency</subject><subject>Oocytes</subject><subject>Oxidoreductions</subject><subject>Redox properties</subject><subject>Reductases</subject><subject>Reduction</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Transport</subject><subject>vacuole</subject><subject>Vacuoles</subject><subject>Vegetal Biology</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kctu1DAUhi0EokNhwRtYYoXUtL7FiZejchmkUemilbqzThyn48qJg-2UzvPwongYBKt6c2Sf73yyzo_Qe0rOaTkX07w7p03N5Au0okKqqqW8eYlWhLC2kkLenaA3KT0QQlQt2Wt0wgVpCSNkhX6t86ebO1afYcCjHTsbcRhw3lk8Lj67Pi73GKYe5_DkDDZhnMNSrvYpxyW5MOEBRuf3Z9ilYngEswQPEUMyIXaQLc4RpjSHmIs57yAXx5Rj8Am7UsFY75fDhItFZvbGu-m-tPA6Quf6MCeX3qJXA_hk3_2tp-j2y-eby021_f712-V6WxmulKw6rgjpZc-tKDtoBDXAbCtFq5RQousIl_1A2WCUUgMXxlhqTT00UJumA0L4Kfp49O7A6zm6EeJeB3B6s97qwxvhrG65aB9pYT8c2TmGH4tNWT-EJU7le5rVdaNaWjL4bzQxpBTt8E9LiT5Ep0t0-k90hb04sj-dt_vnQX11vTlO_AbVW5sJ</recordid><startdate>202109</startdate><enddate>202109</enddate><creator>Hoang, Minh Thi Thanh</creator><creator>Almeida, Diego</creator><creator>Chay, Sandrine</creator><creator>Alcon, Carine</creator><creator>Corratge‐Faillie, Claire</creator><creator>Curie, Catherine</creator><creator>Mari, Stephane</creator><general>Wiley Subscription Services, Inc</general><general>Wiley</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-5413-7844</orcidid><orcidid>https://orcid.org/0000-0002-8884-1175</orcidid><orcidid>https://orcid.org/0000-0001-7040-8253</orcidid><orcidid>https://orcid.org/0000-0003-3296-5029</orcidid><orcidid>https://orcid.org/0000-0002-5738-6712</orcidid></search><sort><creationdate>202109</creationdate><title>AtDTX25, a member of the multidrug and toxic compound extrusion family, is a vacuolar ascorbate transporter that controls intracellular iron cycling in Arabidopsis</title><author>Hoang, Minh Thi Thanh ; Almeida, Diego ; Chay, Sandrine ; Alcon, Carine ; Corratge‐Faillie, Claire ; Curie, Catherine ; Mari, Stephane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3996-b3900d6d3e4469741ca2e864899494bb036df12fc999f34cce1ec5f7a5c7ba003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal embryos</topic><topic>Arabidopsis</topic><topic>ascorbate</topic><topic>Ascorbic acid</topic><topic>Efflux</topic><topic>Embryos</topic><topic>Extrusion</topic><topic>Gametocytes</topic><topic>Germination</topic><topic>Iron</topic><topic>Iron deficiency</topic><topic>Life Sciences</topic><topic>MATE transporter</topic><topic>Membrane proteins</topic><topic>Membranes</topic><topic>Mutants</topic><topic>Nutrient deficiency</topic><topic>Oocytes</topic><topic>Oxidoreductions</topic><topic>Redox properties</topic><topic>Reductases</topic><topic>Reduction</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Transport</topic><topic>vacuole</topic><topic>Vacuoles</topic><topic>Vegetal Biology</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoang, Minh Thi Thanh</creatorcontrib><creatorcontrib>Almeida, Diego</creatorcontrib><creatorcontrib>Chay, Sandrine</creatorcontrib><creatorcontrib>Alcon, Carine</creatorcontrib><creatorcontrib>Corratge‐Faillie, Claire</creatorcontrib><creatorcontrib>Curie, Catherine</creatorcontrib><creatorcontrib>Mari, Stephane</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoang, Minh Thi Thanh</au><au>Almeida, Diego</au><au>Chay, Sandrine</au><au>Alcon, Carine</au><au>Corratge‐Faillie, Claire</au><au>Curie, Catherine</au><au>Mari, Stephane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AtDTX25, a member of the multidrug and toxic compound extrusion family, is a vacuolar ascorbate transporter that controls intracellular iron cycling in Arabidopsis</atitle><jtitle>The New phytologist</jtitle><date>2021-09</date><risdate>2021</risdate><volume>231</volume><issue>5</issue><spage>1956</spage><epage>1967</epage><pages>1956-1967</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Summary
Iron (Fe) is an essential element, its transport is regulated by the cell redox balance. In seeds, Fe enters the embryo as Fe2+ and is stored in vacuoles as Fe3+. Through its ferric reduction activity, ascorbate plays a major role in Fe redox state and therefore Fe transport within the seed.
We searched for ascorbate membrane transporters responsible for controlling Fe reduction by screening the yeast ferric reductase‐deficient fre1 strain and isolated AtDTX25, a member of the Multidrug And Toxic compound Extrusion (MATE) family.
AtDTX25 was shown to mediate ascorbate efflux when expressed in yeast and Xenopus oocytes, in a pH‐dependent manner. In planta, AtDTX25 is highly expressed during germination and encodes a vacuolar membrane protein. Isolated vacuoles from AtDTX25‐1 knockout mutant contained less ascorbate and more Fe than wild‐type (WT), and mutant seedlings were highly sensitive to Fe deficiency. Iron imaging further showed that the remobilisation of Fe from vacuoles was highly impaired in mutant seedlings.
Taken together, our results established AtDTX25 as a vacuolar ascorbate transporter, required during germination to promote the reduction of the pool of stored Fe3+ and its remobilisation to feed the developing seedling.</abstract><cop>Lancaster</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34080200</pmid><doi>10.1111/nph.17526</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-5413-7844</orcidid><orcidid>https://orcid.org/0000-0002-8884-1175</orcidid><orcidid>https://orcid.org/0000-0001-7040-8253</orcidid><orcidid>https://orcid.org/0000-0003-3296-5029</orcidid><orcidid>https://orcid.org/0000-0002-5738-6712</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | The New phytologist, 2021-09, Vol.231 (5), p.1956-1967 |
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| subjects | Animal embryos Arabidopsis ascorbate Ascorbic acid Efflux Embryos Extrusion Gametocytes Germination Iron Iron deficiency Life Sciences MATE transporter Membrane proteins Membranes Mutants Nutrient deficiency Oocytes Oxidoreductions Redox properties Reductases Reduction Seedlings Seeds Transport vacuole Vacuoles Vegetal Biology Yeast Yeasts |
| title | AtDTX25, a member of the multidrug and toxic compound extrusion family, is a vacuolar ascorbate transporter that controls intracellular iron cycling in Arabidopsis |
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