Development and standardization of a specific real-time PCR assay for the rapid detection of Candida auris
Candida auris is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of C. auris , based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. Thi...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2021-07, Vol.40 (7), p.1547-1551 |
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| container_title | European journal of clinical microbiology & infectious diseases |
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| creator | Ibrahim, Ahmad Baron, Sophie Alexandra Yousfi, Hanane Hadjadj, Linda Lalaoui, Rym Morand, Serge Rolain, Jean-Marc Bittar, Fadi |
| description | Candida auris
is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of
C. auris
, based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. This system is reproducible and sensitive with a limit of detection of 13
C. auris
CFU/qPCR reaction. The 100% specificity of this system is confirmed on 2073 clinical and environmental samples, 50 different bacterial species, and 9
Candida
spp. (70 strains). This system is suitable to correctly identify
C. auris
infections and to trace its source. |
| doi_str_mv | 10.1007/s10096-021-04176-8 |
| format | Article |
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is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of
C. auris
, based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. This system is reproducible and sensitive with a limit of detection of 13
C. auris
CFU/qPCR reaction. The 100% specificity of this system is confirmed on 2073 clinical and environmental samples, 50 different bacterial species, and 9
Candida
spp. (70 strains). This system is suitable to correctly identify
C. auris
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is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of
C. auris
, based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. This system is reproducible and sensitive with a limit of detection of 13
C. auris
CFU/qPCR reaction. The 100% specificity of this system is confirmed on 2073 clinical and environmental samples, 50 different bacterial species, and 9
Candida
spp. (70 strains). This system is suitable to correctly identify
C. auris
infections and to trace its source.</description><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brief Report</subject><subject>Candida auris</subject><subject>Cardiology and cardiovascular system</subject><subject>Emerging diseases</subject><subject>Fungal infections</subject><subject>Glycosylphosphatidylinositol</subject><subject>Gram-negative bacteria</subject><subject>Gram-positive bacteria</subject><subject>Hospitals</subject><subject>Human health and pathology</subject><subject>Infectious diseases</subject><subject>Internal Medicine</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Nosocomial infection</subject><subject>Parasitology</subject><subject>Phosphatidylinositol</subject><subject>Standardization</subject><subject>Streptococcus infections</subject><subject>Virology</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9v3CAQxVHVqtls-wV6qJB6aQ6kwICxj9H2Tyqt1KpqzwgDbljZxgU7Uvrpw9ZJKuWQy4wEv_eG4SH0htFzRqn6kEttKkI5I1QwVZH6GdowAZIIUPAcbWgDgjSKwwk6zflAi6hW6iU6AZBMFu0GHT76a9_HafDjjM3ocJ5LNcmFv2YOccSxwwbnydvQBYuTNz2Zw-Dx990PbHI2N7iLCc9XHiczBYedn729V-6KV3AGmyWF_Aq96Eyf_eu7vkW_Pn_6ubsk-29fvu4u9sQK2syESw6Mc9c6xW3TNoK1qpLOeAu27AuqrrumEkwyB7aWTNCWAijaCW-cqyRs0dnqe2V6PaUwmHSjown68mKvj2cUOKuogmtW2PcrO6X4Z_F51kPI1ve9GX1csuaihrp8aWlb9O4ReohLGssmmkvBGAclVaH4StkUc06-e3gBo_qYml5T0yU1_S81fbR-e2e9tIN3D5L7mAoAK5DL1fjbp_-zn7C9BTo3n_I</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>Ibrahim, Ahmad</creator><creator>Baron, Sophie Alexandra</creator><creator>Yousfi, Hanane</creator><creator>Hadjadj, Linda</creator><creator>Lalaoui, Rym</creator><creator>Morand, Serge</creator><creator>Rolain, Jean-Marc</creator><creator>Bittar, Fadi</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-4052-344X</orcidid><orcidid>https://orcid.org/0000-0003-3986-7659</orcidid><orcidid>https://orcid.org/0000-0001-5358-1089</orcidid><orcidid>https://orcid.org/0000-0002-2402-4467</orcidid></search><sort><creationdate>20210701</creationdate><title>Development and standardization of a specific real-time PCR assay for the rapid detection of Candida auris</title><author>Ibrahim, Ahmad ; 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is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of
C. auris
, based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. This system is reproducible and sensitive with a limit of detection of 13
C. auris
CFU/qPCR reaction. The 100% specificity of this system is confirmed on 2073 clinical and environmental samples, 50 different bacterial species, and 9
Candida
spp. (70 strains). This system is suitable to correctly identify
C. auris
infections and to trace its source.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33515096</pmid><doi>10.1007/s10096-021-04176-8</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0003-4052-344X</orcidid><orcidid>https://orcid.org/0000-0003-3986-7659</orcidid><orcidid>https://orcid.org/0000-0001-5358-1089</orcidid><orcidid>https://orcid.org/0000-0002-2402-4467</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0934-9723 |
| ispartof | European journal of clinical microbiology & infectious diseases, 2021-07, Vol.40 (7), p.1547-1551 |
| issn | 0934-9723 1435-4373 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03216073v1 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Bacteria Bacteriology Biomedical and Life Sciences Biomedicine Brief Report Candida auris Cardiology and cardiovascular system Emerging diseases Fungal infections Glycosylphosphatidylinositol Gram-negative bacteria Gram-positive bacteria Hospitals Human health and pathology Infectious diseases Internal Medicine Life Sciences Medical Microbiology Microbiology and Parasitology Nosocomial infection Parasitology Phosphatidylinositol Standardization Streptococcus infections Virology |
| title | Development and standardization of a specific real-time PCR assay for the rapid detection of Candida auris |
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