Zebrafish (Danio rerio) larvae as a model for real‐time studies of propagating VHS virus infection, tissue tropism and neutrophil activity
Viral haemorrhagic septicaemia virus (VHSV) is a negative‐sense single‐stranded RNA virus that infects more than 140 different fish species. In this study, zebrafish larvae were employed as in vivo model organisms to investigate progression of disease, the correlation between propagation of the infe...
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| Veröffentlicht in: | Journal of fish diseases 2021-05, Vol.44 (5), p.563-571 |
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| creator | Marana, Moonika Haahr Schmidt, Jacob Günther Biacchesi, Stéphane Lorenzen, Niels Jørgensen, Louise von Gersdorff |
| description | Viral haemorrhagic septicaemia virus (VHSV) is a negative‐sense single‐stranded RNA virus that infects more than 140 different fish species. In this study, zebrafish larvae were employed as in vivo model organisms to investigate progression of disease, the correlation between propagation of the infection and irreversibility of disease, cell tropism and in situ neutrophil activity towards the VHSV‐infected cells. A recombinant VHSV strain, encoding “tomato” fluorescence (rVHSV‐Tomato), was used in zebrafish to be able to follow the progress of the infection in the live host in real‐time. Two‐day‐old zebrafish larvae were injected into the yolk sac with the recombinant virus. The virus titre peaked 96 hr post‐infection in zebrafish larvae kept at 18°C, and correlated with 33% mortality and high morbidity among the larvae. By utilizing the transgenic zebrafish line Tg(fli1:GFP)y1 with fluorescently tagged endothelial cells, we were able to demonstrate that the virus initially infected endothelial cells lining the blood vessels. By observing the rVHSV‐Tomato infection in the neutrophil reporter zebrafish line Tg(MPX:eGFP)i114, we inferred that only a subpopulation of the neutrophils responded to the virus infection. We conclude that the zebrafish larvae are suitable for real‐time studies of VHS virus infections, allowing in vivo dissection of host–virus interactions at the whole organism level. |
| doi_str_mv | 10.1111/jfd.13294 |
| format | Article |
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In this study, zebrafish larvae were employed as in vivo model organisms to investigate progression of disease, the correlation between propagation of the infection and irreversibility of disease, cell tropism and in situ neutrophil activity towards the VHSV‐infected cells. A recombinant VHSV strain, encoding “tomato” fluorescence (rVHSV‐Tomato), was used in zebrafish to be able to follow the progress of the infection in the live host in real‐time. Two‐day‐old zebrafish larvae were injected into the yolk sac with the recombinant virus. The virus titre peaked 96 hr post‐infection in zebrafish larvae kept at 18°C, and correlated with 33% mortality and high morbidity among the larvae. By utilizing the transgenic zebrafish line Tg(fli1:GFP)y1 with fluorescently tagged endothelial cells, we were able to demonstrate that the virus initially infected endothelial cells lining the blood vessels. By observing the rVHSV‐Tomato infection in the neutrophil reporter zebrafish line Tg(MPX:eGFP)i114, we inferred that only a subpopulation of the neutrophils responded to the virus infection. We conclude that the zebrafish larvae are suitable for real‐time studies of VHS virus infections, allowing in vivo dissection of host–virus interactions at the whole organism level.</description><identifier>ISSN: 0140-7775</identifier><identifier>EISSN: 1365-2761</identifier><identifier>DOI: 10.1111/jfd.13294</identifier><identifier>PMID: 33170959</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Animal biology ; Blood vessels ; Danio rerio ; Egg yolk ; Endothelial cells ; Fish ; Fluorescence ; Fluorescence in situ hybridization ; Freshwater fishes ; Hemorrhagic septicemia ; In vivo methods and tests ; in vivo visualization ; infection kinetics ; Infections ; Larvae ; Leukocytes (neutrophilic) ; Life Sciences ; Morbidity ; mortality ; neutrophil response ; Neutrophils ; Recombinants ; RNA viruses ; Septicaemia ; tissue tropism ; Tomatoes ; Tropism ; Tropisms ; Veterinary medicine and animal Health ; viral haemorrhagic septicaemia virus ; Viruses ; Yolk ; Yolk sac ; Zebrafish</subject><ispartof>Journal of fish diseases, 2021-05, Vol.44 (5), p.563-571</ispartof><rights>2020 John Wiley & Sons Ltd</rights><rights>2020 John Wiley & Sons Ltd.</rights><rights>Copyright © 2021 John Wiley & Sons Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3874-5c72819bda47f367c22f07d85a9087969a80a0946464b1fc464ad88b5cc5e4223</citedby><cites>FETCH-LOGICAL-c3874-5c72819bda47f367c22f07d85a9087969a80a0946464b1fc464ad88b5cc5e4223</cites><orcidid>0000-0002-6005-3249 ; 0000-0003-3535-6341 ; 0000-0002-7020-2391</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfd.13294$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfd.13294$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33170959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-03205358$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Marana, Moonika Haahr</creatorcontrib><creatorcontrib>Schmidt, Jacob Günther</creatorcontrib><creatorcontrib>Biacchesi, Stéphane</creatorcontrib><creatorcontrib>Lorenzen, Niels</creatorcontrib><creatorcontrib>Jørgensen, Louise von Gersdorff</creatorcontrib><title>Zebrafish (Danio rerio) larvae as a model for real‐time studies of propagating VHS virus infection, tissue tropism and neutrophil activity</title><title>Journal of fish diseases</title><addtitle>J Fish Dis</addtitle><description>Viral haemorrhagic septicaemia virus (VHSV) is a negative‐sense single‐stranded RNA virus that infects more than 140 different fish species. In this study, zebrafish larvae were employed as in vivo model organisms to investigate progression of disease, the correlation between propagation of the infection and irreversibility of disease, cell tropism and in situ neutrophil activity towards the VHSV‐infected cells. A recombinant VHSV strain, encoding “tomato” fluorescence (rVHSV‐Tomato), was used in zebrafish to be able to follow the progress of the infection in the live host in real‐time. Two‐day‐old zebrafish larvae were injected into the yolk sac with the recombinant virus. The virus titre peaked 96 hr post‐infection in zebrafish larvae kept at 18°C, and correlated with 33% mortality and high morbidity among the larvae. By utilizing the transgenic zebrafish line Tg(fli1:GFP)y1 with fluorescently tagged endothelial cells, we were able to demonstrate that the virus initially infected endothelial cells lining the blood vessels. By observing the rVHSV‐Tomato infection in the neutrophil reporter zebrafish line Tg(MPX:eGFP)i114, we inferred that only a subpopulation of the neutrophils responded to the virus infection. We conclude that the zebrafish larvae are suitable for real‐time studies of VHS virus infections, allowing in vivo dissection of host–virus interactions at the whole organism level.</description><subject>Animal biology</subject><subject>Blood vessels</subject><subject>Danio rerio</subject><subject>Egg yolk</subject><subject>Endothelial cells</subject><subject>Fish</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Freshwater fishes</subject><subject>Hemorrhagic septicemia</subject><subject>In vivo methods and tests</subject><subject>in vivo visualization</subject><subject>infection kinetics</subject><subject>Infections</subject><subject>Larvae</subject><subject>Leukocytes (neutrophilic)</subject><subject>Life Sciences</subject><subject>Morbidity</subject><subject>mortality</subject><subject>neutrophil response</subject><subject>Neutrophils</subject><subject>Recombinants</subject><subject>RNA viruses</subject><subject>Septicaemia</subject><subject>tissue tropism</subject><subject>Tomatoes</subject><subject>Tropism</subject><subject>Tropisms</subject><subject>Veterinary medicine and animal Health</subject><subject>viral haemorrhagic septicaemia virus</subject><subject>Viruses</subject><subject>Yolk</subject><subject>Yolk sac</subject><subject>Zebrafish</subject><issn>0140-7775</issn><issn>1365-2761</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1DAUhS0EokNhwQsgS2xaibT-ieN4WbWUKRqpC34WbKybxO545MRTOxk0Ox6ABc_Ik-DptEVCwndx5etP5_roIPSakhOaz-nKdieUM1U-QTPKK1EwWdGnaEZoSQoppThAL1JaEUKloNVzdMA5lUQJNUM_v5kmgnVpiY8uYHABRxNdOMYe4gYMhoQB96EzHtsQ8yP43z9-ja43OI1T50zCweJ1DGu4gdENN_jr_BPeuDgl7AZr2tGF4R0eXUqTwWPmXOoxDB0ezLS7Lp3HkKmNG7cv0TMLPplX9_0Qfbl8__l8XiyuP1ydny2KlteyLEQrWU1V00EpLa9ky5glsqsFKFJLVSmoCRBVVrkaatvcoKvrRrStMCVj_BAd73WX4PU6uh7iVgdwen620LsZ4YwILuoNzezRns0ebyeTRt271BrvYTBhSpqVQlVMCFln9O0_6CpMcchONBNEMcqEEn-XtzGkFI19_AElehenznHquzgz--ZecWp60z2SD_ll4HQPfHfebP-vpD9eXuwl_wCVaqlp</recordid><startdate>202105</startdate><enddate>202105</enddate><creator>Marana, Moonika Haahr</creator><creator>Schmidt, Jacob Günther</creator><creator>Biacchesi, Stéphane</creator><creator>Lorenzen, Niels</creator><creator>Jørgensen, Louise von Gersdorff</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-6005-3249</orcidid><orcidid>https://orcid.org/0000-0003-3535-6341</orcidid><orcidid>https://orcid.org/0000-0002-7020-2391</orcidid></search><sort><creationdate>202105</creationdate><title>Zebrafish (Danio rerio) larvae as a model for real‐time studies of propagating VHS virus infection, tissue tropism and neutrophil activity</title><author>Marana, Moonika Haahr ; Schmidt, Jacob Günther ; Biacchesi, Stéphane ; Lorenzen, Niels ; Jørgensen, Louise von Gersdorff</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3874-5c72819bda47f367c22f07d85a9087969a80a0946464b1fc464ad88b5cc5e4223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animal biology</topic><topic>Blood vessels</topic><topic>Danio rerio</topic><topic>Egg yolk</topic><topic>Endothelial cells</topic><topic>Fish</topic><topic>Fluorescence</topic><topic>Fluorescence in situ hybridization</topic><topic>Freshwater fishes</topic><topic>Hemorrhagic septicemia</topic><topic>In vivo methods and tests</topic><topic>in vivo visualization</topic><topic>infection kinetics</topic><topic>Infections</topic><topic>Larvae</topic><topic>Leukocytes (neutrophilic)</topic><topic>Life Sciences</topic><topic>Morbidity</topic><topic>mortality</topic><topic>neutrophil response</topic><topic>Neutrophils</topic><topic>Recombinants</topic><topic>RNA viruses</topic><topic>Septicaemia</topic><topic>tissue tropism</topic><topic>Tomatoes</topic><topic>Tropism</topic><topic>Tropisms</topic><topic>Veterinary medicine and animal Health</topic><topic>viral haemorrhagic septicaemia virus</topic><topic>Viruses</topic><topic>Yolk</topic><topic>Yolk sac</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marana, Moonika Haahr</creatorcontrib><creatorcontrib>Schmidt, Jacob Günther</creatorcontrib><creatorcontrib>Biacchesi, Stéphane</creatorcontrib><creatorcontrib>Lorenzen, Niels</creatorcontrib><creatorcontrib>Jørgensen, Louise von Gersdorff</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of fish diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marana, Moonika Haahr</au><au>Schmidt, Jacob Günther</au><au>Biacchesi, Stéphane</au><au>Lorenzen, Niels</au><au>Jørgensen, Louise von Gersdorff</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zebrafish (Danio rerio) larvae as a model for real‐time studies of propagating VHS virus infection, tissue tropism and neutrophil activity</atitle><jtitle>Journal of fish diseases</jtitle><addtitle>J Fish Dis</addtitle><date>2021-05</date><risdate>2021</risdate><volume>44</volume><issue>5</issue><spage>563</spage><epage>571</epage><pages>563-571</pages><issn>0140-7775</issn><eissn>1365-2761</eissn><abstract>Viral haemorrhagic septicaemia virus (VHSV) is a negative‐sense single‐stranded RNA virus that infects more than 140 different fish species. In this study, zebrafish larvae were employed as in vivo model organisms to investigate progression of disease, the correlation between propagation of the infection and irreversibility of disease, cell tropism and in situ neutrophil activity towards the VHSV‐infected cells. A recombinant VHSV strain, encoding “tomato” fluorescence (rVHSV‐Tomato), was used in zebrafish to be able to follow the progress of the infection in the live host in real‐time. Two‐day‐old zebrafish larvae were injected into the yolk sac with the recombinant virus. The virus titre peaked 96 hr post‐infection in zebrafish larvae kept at 18°C, and correlated with 33% mortality and high morbidity among the larvae. By utilizing the transgenic zebrafish line Tg(fli1:GFP)y1 with fluorescently tagged endothelial cells, we were able to demonstrate that the virus initially infected endothelial cells lining the blood vessels. By observing the rVHSV‐Tomato infection in the neutrophil reporter zebrafish line Tg(MPX:eGFP)i114, we inferred that only a subpopulation of the neutrophils responded to the virus infection. We conclude that the zebrafish larvae are suitable for real‐time studies of VHS virus infections, allowing in vivo dissection of host–virus interactions at the whole organism level.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>33170959</pmid><doi>10.1111/jfd.13294</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-6005-3249</orcidid><orcidid>https://orcid.org/0000-0003-3535-6341</orcidid><orcidid>https://orcid.org/0000-0002-7020-2391</orcidid></addata></record> |
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| subjects | Animal biology Blood vessels Danio rerio Egg yolk Endothelial cells Fish Fluorescence Fluorescence in situ hybridization Freshwater fishes Hemorrhagic septicemia In vivo methods and tests in vivo visualization infection kinetics Infections Larvae Leukocytes (neutrophilic) Life Sciences Morbidity mortality neutrophil response Neutrophils Recombinants RNA viruses Septicaemia tissue tropism Tomatoes Tropism Tropisms Veterinary medicine and animal Health viral haemorrhagic septicaemia virus Viruses Yolk Yolk sac Zebrafish |
| title | Zebrafish (Danio rerio) larvae as a model for real‐time studies of propagating VHS virus infection, tissue tropism and neutrophil activity |
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