Assessment of Complement Activation by Nanoparticles: Development of a SPR Based Method and Comparison with Current High Throughput Methods
Purpose A Surface Plasmon Resonance chip (SPR) was developed to study the activation of complement system triggered by nanomaterials in contact with human serum, which is an important concern today to warrant safety of nanomedicines. Methods The developed chip was tested for its specificity in compl...
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| Veröffentlicht in: | Pharmaceutical research 2018-07, Vol.35 (7), p.129-11, Article 129 |
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| creator | Coty, Jean-Baptiste Noiray, Magali Vauthier, Christine |
| description | Purpose
A Surface Plasmon Resonance chip (SPR) was developed to study the activation of complement system triggered by nanomaterials in contact with human serum, which is an important concern today to warrant safety of nanomedicines.
Methods
The developed chip was tested for its specificity in complex medium and its longevity of use. It was then employed to assess the release of complement fragments upon incubation of nanoparticles in serum. A comparison was made with other current methods assessing complement activation (μC-IE, ELISA).
Results
The SPR chip was found to give a consistent response for C3a release upon activation by nanoparticles. Results were similar to those obtained by μC-IE. However, ELISA detection of iC3b fragments showed an explained high non-specific background. The impact of sample preparation preceding the analysis was assessed with the newly develop SPR method. The removal of nanoparticles before analysis showed an important modification in the obtained response, possibly leading to false negative results.
Conclusion
The SPR chip developed in this work allows for an automated assessment of complement activation triggered by nanoparticles with possibility of multiplexed analysis. The design of the chip proved to give consistent results of complement activation by nanoparticles. |
| doi_str_mv | 10.1007/s11095-018-2406-5 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_03199958v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A724210201</galeid><sourcerecordid>A724210201</sourcerecordid><originalsourceid>FETCH-LOGICAL-c488t-7c3257d8801be55716f702524d0ea81f2270d32d7db21ab17fcdfe33eb75ae413</originalsourceid><addsrcrecordid>eNp1kl2L1DAUhoso7rj6A7yRgDd60fUkbSetd3X8GGH8QFfwLqTN6TRL23STdGR_g3_adDq7giC5CDl53pdzOG8UPaVwQQH4K0cpFFkMNI9ZCus4uxetaMaTuID05_1oBZylcc5TehY9cu4KAHJapA-jM1ZwgDVjq-h36Rw61-PgiWnIxvRjh8dXWXt9kF6bgVQ35LMczCit13WH7jV5iwfszHgrk-T712_kjXSoyCf0rVFEDuroJq12weKX9i3ZTNbOiq3et-SytWbat-PkTxL3OHrQyM7hk9N9Hv14_-5ys413Xz583JS7uE7z3Me8TljGVZ4DrTDLOF03HFjGUgUoc9owxkElTHFVMSoryptaNZgkWPFMYkqT8-jl4tvKToxW99LeCCO12JY7MdcgoUVRZPlhZl8s7GjN9YTOi167GrtODmgmJxgkLE0SYEVAn_-DXpnJDmGSmaIBS2E2vFiovexQ6KEx3so6HIW9rs2AjQ71MmyOUWBHAV0EtTXOWWzuWqYg5hiIJQYixEDMMRBZ0Dw7tTJVPao7xe3eA8AWwIWvYY_2b6__d_0DZl68Uw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2031243401</pqid></control><display><type>article</type><title>Assessment of Complement Activation by Nanoparticles: Development of a SPR Based Method and Comparison with Current High Throughput Methods</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Coty, Jean-Baptiste ; Noiray, Magali ; Vauthier, Christine</creator><creatorcontrib>Coty, Jean-Baptiste ; Noiray, Magali ; Vauthier, Christine</creatorcontrib><description>Purpose
A Surface Plasmon Resonance chip (SPR) was developed to study the activation of complement system triggered by nanomaterials in contact with human serum, which is an important concern today to warrant safety of nanomedicines.
Methods
The developed chip was tested for its specificity in complex medium and its longevity of use. It was then employed to assess the release of complement fragments upon incubation of nanoparticles in serum. A comparison was made with other current methods assessing complement activation (μC-IE, ELISA).
Results
The SPR chip was found to give a consistent response for C3a release upon activation by nanoparticles. Results were similar to those obtained by μC-IE. However, ELISA detection of iC3b fragments showed an explained high non-specific background. The impact of sample preparation preceding the analysis was assessed with the newly develop SPR method. The removal of nanoparticles before analysis showed an important modification in the obtained response, possibly leading to false negative results.
Conclusion
The SPR chip developed in this work allows for an automated assessment of complement activation triggered by nanoparticles with possibility of multiplexed analysis. The design of the chip proved to give consistent results of complement activation by nanoparticles.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1007/s11095-018-2406-5</identifier><identifier>PMID: 29700622</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Activation analysis ; Analysis ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedical Engineering and Bioengineering ; Biomedicine ; Complement activation ; Complement C3 - agonists ; Complement C3 - metabolism ; Electrophoresis - methods ; Enzyme-linked immunosorbent assay ; Ethylenediaminetetraacetic acid ; Fragments ; Goats ; High-Throughput Screening Assays - methods ; Humans ; Immunology ; Innate immunity ; Life Sciences ; Medical Law ; Methods ; Mice ; Nanoparticles ; Nanoparticles - administration & dosage ; Nanoparticles - metabolism ; Nanotechnology ; Pharmaceutical sciences ; Pharmacology ; Pharmacology/Toxicology ; Pharmacy ; Research Paper ; Sample preparation ; Surface plasmon resonance ; Surface Plasmon Resonance - methods</subject><ispartof>Pharmaceutical research, 2018-07, Vol.35 (7), p.129-11, Article 129</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Pharmaceutical Research is a copyright of Springer, (2018). All Rights Reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-7c3257d8801be55716f702524d0ea81f2270d32d7db21ab17fcdfe33eb75ae413</citedby><cites>FETCH-LOGICAL-c488t-7c3257d8801be55716f702524d0ea81f2270d32d7db21ab17fcdfe33eb75ae413</cites><orcidid>0000-0003-3963-0045 ; 0000-0003-4769-9683</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11095-018-2406-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11095-018-2406-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29700622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03199958$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Coty, Jean-Baptiste</creatorcontrib><creatorcontrib>Noiray, Magali</creatorcontrib><creatorcontrib>Vauthier, Christine</creatorcontrib><title>Assessment of Complement Activation by Nanoparticles: Development of a SPR Based Method and Comparison with Current High Throughput Methods</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><addtitle>Pharm Res</addtitle><description>Purpose
A Surface Plasmon Resonance chip (SPR) was developed to study the activation of complement system triggered by nanomaterials in contact with human serum, which is an important concern today to warrant safety of nanomedicines.
Methods
The developed chip was tested for its specificity in complex medium and its longevity of use. It was then employed to assess the release of complement fragments upon incubation of nanoparticles in serum. A comparison was made with other current methods assessing complement activation (μC-IE, ELISA).
Results
The SPR chip was found to give a consistent response for C3a release upon activation by nanoparticles. Results were similar to those obtained by μC-IE. However, ELISA detection of iC3b fragments showed an explained high non-specific background. The impact of sample preparation preceding the analysis was assessed with the newly develop SPR method. The removal of nanoparticles before analysis showed an important modification in the obtained response, possibly leading to false negative results.
Conclusion
The SPR chip developed in this work allows for an automated assessment of complement activation triggered by nanoparticles with possibility of multiplexed analysis. The design of the chip proved to give consistent results of complement activation by nanoparticles.</description><subject>Activation analysis</subject><subject>Analysis</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedicine</subject><subject>Complement activation</subject><subject>Complement C3 - agonists</subject><subject>Complement C3 - metabolism</subject><subject>Electrophoresis - methods</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fragments</subject><subject>Goats</subject><subject>High-Throughput Screening Assays - methods</subject><subject>Humans</subject><subject>Immunology</subject><subject>Innate immunity</subject><subject>Life Sciences</subject><subject>Medical Law</subject><subject>Methods</subject><subject>Mice</subject><subject>Nanoparticles</subject><subject>Nanoparticles - administration & dosage</subject><subject>Nanoparticles - metabolism</subject><subject>Nanotechnology</subject><subject>Pharmaceutical sciences</subject><subject>Pharmacology</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Research Paper</subject><subject>Sample preparation</subject><subject>Surface plasmon resonance</subject><subject>Surface Plasmon Resonance - methods</subject><issn>0724-8741</issn><issn>1573-904X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kl2L1DAUhoso7rj6A7yRgDd60fUkbSetd3X8GGH8QFfwLqTN6TRL23STdGR_g3_adDq7giC5CDl53pdzOG8UPaVwQQH4K0cpFFkMNI9ZCus4uxetaMaTuID05_1oBZylcc5TehY9cu4KAHJapA-jM1ZwgDVjq-h36Rw61-PgiWnIxvRjh8dXWXt9kF6bgVQ35LMczCit13WH7jV5iwfszHgrk-T712_kjXSoyCf0rVFEDuroJq12weKX9i3ZTNbOiq3et-SytWbat-PkTxL3OHrQyM7hk9N9Hv14_-5ys413Xz583JS7uE7z3Me8TljGVZ4DrTDLOF03HFjGUgUoc9owxkElTHFVMSoryptaNZgkWPFMYkqT8-jl4tvKToxW99LeCCO12JY7MdcgoUVRZPlhZl8s7GjN9YTOi167GrtODmgmJxgkLE0SYEVAn_-DXpnJDmGSmaIBS2E2vFiovexQ6KEx3so6HIW9rs2AjQ71MmyOUWBHAV0EtTXOWWzuWqYg5hiIJQYixEDMMRBZ0Dw7tTJVPao7xe3eA8AWwIWvYY_2b6__d_0DZl68Uw</recordid><startdate>20180701</startdate><enddate>20180701</enddate><creator>Coty, Jean-Baptiste</creator><creator>Noiray, Magali</creator><creator>Vauthier, Christine</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><general>American Association of Pharmaceutical Scientists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0003-3963-0045</orcidid><orcidid>https://orcid.org/0000-0003-4769-9683</orcidid></search><sort><creationdate>20180701</creationdate><title>Assessment of Complement Activation by Nanoparticles: Development of a SPR Based Method and Comparison with Current High Throughput Methods</title><author>Coty, Jean-Baptiste ; Noiray, Magali ; Vauthier, Christine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-7c3257d8801be55716f702524d0ea81f2270d32d7db21ab17fcdfe33eb75ae413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Activation analysis</topic><topic>Analysis</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedicine</topic><topic>Complement activation</topic><topic>Complement C3 - agonists</topic><topic>Complement C3 - metabolism</topic><topic>Electrophoresis - methods</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fragments</topic><topic>Goats</topic><topic>High-Throughput Screening Assays - methods</topic><topic>Humans</topic><topic>Immunology</topic><topic>Innate immunity</topic><topic>Life Sciences</topic><topic>Medical Law</topic><topic>Methods</topic><topic>Mice</topic><topic>Nanoparticles</topic><topic>Nanoparticles - administration & dosage</topic><topic>Nanoparticles - metabolism</topic><topic>Nanotechnology</topic><topic>Pharmaceutical sciences</topic><topic>Pharmacology</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Research Paper</topic><topic>Sample preparation</topic><topic>Surface plasmon resonance</topic><topic>Surface Plasmon Resonance - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coty, Jean-Baptiste</creatorcontrib><creatorcontrib>Noiray, Magali</creatorcontrib><creatorcontrib>Vauthier, Christine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coty, Jean-Baptiste</au><au>Noiray, Magali</au><au>Vauthier, Christine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of Complement Activation by Nanoparticles: Development of a SPR Based Method and Comparison with Current High Throughput Methods</atitle><jtitle>Pharmaceutical research</jtitle><stitle>Pharm Res</stitle><addtitle>Pharm Res</addtitle><date>2018-07-01</date><risdate>2018</risdate><volume>35</volume><issue>7</issue><spage>129</spage><epage>11</epage><pages>129-11</pages><artnum>129</artnum><issn>0724-8741</issn><eissn>1573-904X</eissn><abstract>Purpose
A Surface Plasmon Resonance chip (SPR) was developed to study the activation of complement system triggered by nanomaterials in contact with human serum, which is an important concern today to warrant safety of nanomedicines.
Methods
The developed chip was tested for its specificity in complex medium and its longevity of use. It was then employed to assess the release of complement fragments upon incubation of nanoparticles in serum. A comparison was made with other current methods assessing complement activation (μC-IE, ELISA).
Results
The SPR chip was found to give a consistent response for C3a release upon activation by nanoparticles. Results were similar to those obtained by μC-IE. However, ELISA detection of iC3b fragments showed an explained high non-specific background. The impact of sample preparation preceding the analysis was assessed with the newly develop SPR method. The removal of nanoparticles before analysis showed an important modification in the obtained response, possibly leading to false negative results.
Conclusion
The SPR chip developed in this work allows for an automated assessment of complement activation triggered by nanoparticles with possibility of multiplexed analysis. The design of the chip proved to give consistent results of complement activation by nanoparticles.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>29700622</pmid><doi>10.1007/s11095-018-2406-5</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3963-0045</orcidid><orcidid>https://orcid.org/0000-0003-4769-9683</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Activation analysis Analysis Animals Biochemistry Biomedical and Life Sciences Biomedical Engineering and Bioengineering Biomedicine Complement activation Complement C3 - agonists Complement C3 - metabolism Electrophoresis - methods Enzyme-linked immunosorbent assay Ethylenediaminetetraacetic acid Fragments Goats High-Throughput Screening Assays - methods Humans Immunology Innate immunity Life Sciences Medical Law Methods Mice Nanoparticles Nanoparticles - administration & dosage Nanoparticles - metabolism Nanotechnology Pharmaceutical sciences Pharmacology Pharmacology/Toxicology Pharmacy Research Paper Sample preparation Surface plasmon resonance Surface Plasmon Resonance - methods |
| title | Assessment of Complement Activation by Nanoparticles: Development of a SPR Based Method and Comparison with Current High Throughput Methods |
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