Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila
Use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to introduce RNA-protein crosslinks in the 40S and 60S subunits of the cytoplasmic ribosome of Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompan...
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| Veröffentlicht in: | Biochimie 1989-05, Vol.71 (5), p.667-679 |
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| container_end_page | 679 |
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| container_title | Biochimie |
| container_volume | 71 |
| creator | Petridou, Barbara Guerin, Marie-France Hayes, Françoise |
| description | Use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide to introduce RNA-protein crosslinks in the 40S and 60S subunits of the cytoplasmic ribosome of
Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompanied by extensive dimerization and aggregation of ribosomal subunits probably due to formation of interparticle protein-protein crosslinks. |
| doi_str_mv | 10.1016/0300-9084(89)90161-2 |
| format | Article |
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Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompanied by extensive dimerization and aggregation of ribosomal subunits probably due to formation of interparticle protein-protein crosslinks.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>carbodiimide</subject><subject>Cell structures and functions</subject><subject>Centrifugation, Density Gradient</subject><subject>Cross-Linking Reagents</subject><subject>crosslinking</subject><subject>Cytoplasm - metabolism</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Ethyldimethylaminopropyl Carbodiimide</subject><subject>Fundamental and applied biological sciences. 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Tetrahymena thermophila is described, and proteins linked covalently to 17S and 26S ribosomal RNAs are identified. RNA-protein crosslinking is accompanied by extensive dimerization and aggregation of ribosomal subunits probably due to formation of interparticle protein-protein crosslinks.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>2475179</pmid><doi>10.1016/0300-9084(89)90161-2</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9084 |
| ispartof | Biochimie, 1989-05, Vol.71 (5), p.667-679 |
| issn | 0300-9084 1638-6183 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences carbodiimide Cell structures and functions Centrifugation, Density Gradient Cross-Linking Reagents crosslinking Cytoplasm - metabolism Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Ethyldimethylaminopropyl Carbodiimide Fundamental and applied biological sciences. Psychology Life Sciences Molecular and cellular biology Peptide Mapping Ribosomal Proteins - metabolism Ribosome Ribosomes - drug effects Ribosomes - metabolism RNA - metabolism Tetrahymena Tetrahymena - drug effects Tetrahymena - metabolism |
| title | Protein-RNA crosslinking in the subunits of the cytoplasmic ribosome of Tetrahymena thermophila |
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