Validation of quantitative real-time RT-PCR assays for the detection of six honeybee viruses

•The accuracy of 5 RT-qPCRs was assessed for their ability to quantify Europe’s most frequently-detected viruses.•Honeybees collected in France (2012–2019) were tested to determine the frequency of each viral load.•Viral load thresholds were suggested to differentiate covert from overt infections. A...

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Veröffentlicht in:	Journal of virological methods 2019-08, Vol.270, p.70-78
Hauptverfasser:	Schurr, Frank, Tison, Amandine, Militano, Laura, Cheviron, Nicolas, Sircoulomb, Fabrice, Rivière, Marie-Pierre, Ribière-Chabert, Magali, Thiéry, Richard, Dubois, Eric
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal biology Diagnostic threshold Fluorogenic probe Life Sciences Precision Quantitative RT-PCR Trueness Veterinary medicine and animal Health
Online-Zugang:	Volltext
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