A novel model to study the dorsolateral migration of melanoblasts
Melanocytes derived from pluripotent neural crest cells migrate initially in the dorsolateral pathway between the ectoderm and dermomyotome. To understand the role of specific proteins involved in this cell migration, we looked for a cellular model that mimics the in vivo behavior of melanoblasts, a...
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| Veröffentlicht in: | Mechanisms of development 1999-12, Vol.89 (1), p.3-14 |
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| creator | Beauvais-Jouneau, Alice Pla, Patrick Bernex, Florence Dufour, Sylvie Salamero, Jean Fässler, Reinhard Panthier, Jean-Jacques Thiery, Jean Paul Larue, Lionel |
| description | Melanocytes derived from pluripotent neural crest cells migrate initially in the dorsolateral pathway between the ectoderm and dermomyotome. To understand the role of specific proteins involved in this cell migration, we looked for a cellular model that mimics the in vivo behavior of melanoblasts, and that allows functional studies of their migration. We report here that wild-type embryonic stem (ES) cells are able to follow the ventral and dorsolateral neural crest pathways after being grafted into chicken embryos. By contrast, a mutant ES cell line deficient for
β1 integrin subunits, proteins involved in cell–extracellular interactions, had a severely impaired migratory behavior. Interestingly, ES cells deficient for Kit, the tyrosine kinase receptor for the stem cell factor (SCF), behaved similarly to wild-type ES cells. Thus, grafting mouse ES cells into chicken embryos provides a new cellular system that allows both in vitro and in vivo studies of the molecular mechanisms controlling dorsolateral migration. |
| doi_str_mv | 10.1016/S0925-4773(99)00191-4 |
| format | Article |
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β1 integrin subunits, proteins involved in cell–extracellular interactions, had a severely impaired migratory behavior. Interestingly, ES cells deficient for Kit, the tyrosine kinase receptor for the stem cell factor (SCF), behaved similarly to wild-type ES cells. Thus, grafting mouse ES cells into chicken embryos provides a new cellular system that allows both in vitro and in vivo studies of the molecular mechanisms controlling dorsolateral migration.</description><identifier>ISSN: 0925-4773</identifier><identifier>EISSN: 1872-6356</identifier><identifier>DOI: 10.1016/S0925-4773(99)00191-4</identifier><identifier>PMID: 10559475</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Binding Sites ; Biomarkers ; Cell Line ; Cell Movement - physiology ; Chick Embryo ; DNA-Binding Proteins - genetics ; Embryo ; Embryonic Induction ; Embryonic stem cell ; Endothelin-3 - genetics ; Fibronectins - metabolism ; Fluorescent Dyes - metabolism ; Gene Expression Regulation, Developmental ; Integrin beta1 - genetics ; Integrin beta1 - metabolism ; Intramolecular Oxidoreductases - genetics ; Life Sciences ; Melanocyte ; Melanocytes - physiology ; Membrane Glycoproteins ; Mice ; Mice, Mutant Strains ; Microphthalmia-Associated Transcription Factor ; Monophenol Monooxygenase - genetics ; Mutation ; Nervous System - cytology ; Nervous System - embryology ; Neural crest ; Oxidoreductases ; Pigmented cell ; Proteins - genetics ; Proto-Oncogene Proteins c-kit - genetics ; Receptor, Endothelin B ; Receptors, Endothelin - genetics ; Snail Family Transcription Factors ; Stem Cell Transplantation ; Transcription Factors - genetics ; Xenograft ; β1 integrin</subject><ispartof>Mechanisms of development, 1999-12, Vol.89 (1), p.3-14</ispartof><rights>1999 Elsevier Science Ireland Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-825e3fecb033fd98b05f6156aad8186e0c38d322efed6e5a91d4197214d361783</citedby><cites>FETCH-LOGICAL-c442t-825e3fecb033fd98b05f6156aad8186e0c38d322efed6e5a91d4197214d361783</cites><orcidid>0000-0002-0145-6937 ; 0000-0002-5957-2340 ; 0000-0003-0366-4315 ; 0000-0002-8646-4935 ; 0000-0002-7966-0663 ; 0000-0002-2010-6107 ; 0000-0003-0478-5020</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0925-4773(99)00191-4$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10559475$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03110414$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Beauvais-Jouneau, Alice</creatorcontrib><creatorcontrib>Pla, Patrick</creatorcontrib><creatorcontrib>Bernex, Florence</creatorcontrib><creatorcontrib>Dufour, Sylvie</creatorcontrib><creatorcontrib>Salamero, Jean</creatorcontrib><creatorcontrib>Fässler, Reinhard</creatorcontrib><creatorcontrib>Panthier, Jean-Jacques</creatorcontrib><creatorcontrib>Thiery, Jean Paul</creatorcontrib><creatorcontrib>Larue, Lionel</creatorcontrib><title>A novel model to study the dorsolateral migration of melanoblasts</title><title>Mechanisms of development</title><addtitle>Mech Dev</addtitle><description>Melanocytes derived from pluripotent neural crest cells migrate initially in the dorsolateral pathway between the ectoderm and dermomyotome. To understand the role of specific proteins involved in this cell migration, we looked for a cellular model that mimics the in vivo behavior of melanoblasts, and that allows functional studies of their migration. We report here that wild-type embryonic stem (ES) cells are able to follow the ventral and dorsolateral neural crest pathways after being grafted into chicken embryos. By contrast, a mutant ES cell line deficient for
β1 integrin subunits, proteins involved in cell–extracellular interactions, had a severely impaired migratory behavior. Interestingly, ES cells deficient for Kit, the tyrosine kinase receptor for the stem cell factor (SCF), behaved similarly to wild-type ES cells. Thus, grafting mouse ES cells into chicken embryos provides a new cellular system that allows both in vitro and in vivo studies of the molecular mechanisms controlling dorsolateral migration.</description><subject>Animals</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Binding Sites</subject><subject>Biomarkers</subject><subject>Cell Line</subject><subject>Cell Movement - physiology</subject><subject>Chick Embryo</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Embryo</subject><subject>Embryonic Induction</subject><subject>Embryonic stem cell</subject><subject>Endothelin-3 - genetics</subject><subject>Fibronectins - metabolism</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Integrin beta1 - genetics</subject><subject>Integrin beta1 - metabolism</subject><subject>Intramolecular Oxidoreductases - genetics</subject><subject>Life Sciences</subject><subject>Melanocyte</subject><subject>Melanocytes - physiology</subject><subject>Membrane Glycoproteins</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Microphthalmia-Associated Transcription Factor</subject><subject>Monophenol Monooxygenase - genetics</subject><subject>Mutation</subject><subject>Nervous System - cytology</subject><subject>Nervous System - embryology</subject><subject>Neural crest</subject><subject>Oxidoreductases</subject><subject>Pigmented cell</subject><subject>Proteins - genetics</subject><subject>Proto-Oncogene Proteins c-kit - genetics</subject><subject>Receptor, Endothelin B</subject><subject>Receptors, Endothelin - genetics</subject><subject>Snail Family Transcription Factors</subject><subject>Stem Cell Transplantation</subject><subject>Transcription Factors - genetics</subject><subject>Xenograft</subject><subject>β1 integrin</subject><issn>0925-4773</issn><issn>1872-6356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLJDEQgMOirKO7P0HpkziHXlN5deckg_hYGPDg7jlkOtUa6Z5okhnw35uxRbx5qYKqrx58hBwD_QMU1Pk91UzWomn4mdZzSkFDLX6QGbQNqxWXao_MPpEDcpjSEy0UKPhJDoBKqUUjZ2SxqNZhi0M1BldiDlXKG_da5UesXIgpDDZjtKXvH6LNPqyr0FcjDnYdVoNNOf0i-70dEv7-yEfk__XVv8vbenl38_dysaw7IViuWyaR99itKOe90-2Kyl6BVNa6FlqFtOOt44xhj06htBqcAN0wEI4raFp-RObT3kc7mOfoRxtfTbDe3C6WZlejHIAKEFso7OnEPsfwssGUzehTh0P5GsMmGaVZw7iUBZQT2MWQUsT-czNQs_Ns3j2bnUSjtXn3bESZO_k4sFmN6L5MTWILcDEBWJRsPUaTOo_rDp2P2GXjgv_mxBvP8IuK</recordid><startdate>19991201</startdate><enddate>19991201</enddate><creator>Beauvais-Jouneau, Alice</creator><creator>Pla, Patrick</creator><creator>Bernex, Florence</creator><creator>Dufour, Sylvie</creator><creator>Salamero, Jean</creator><creator>Fässler, Reinhard</creator><creator>Panthier, Jean-Jacques</creator><creator>Thiery, Jean Paul</creator><creator>Larue, Lionel</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-0145-6937</orcidid><orcidid>https://orcid.org/0000-0002-5957-2340</orcidid><orcidid>https://orcid.org/0000-0003-0366-4315</orcidid><orcidid>https://orcid.org/0000-0002-8646-4935</orcidid><orcidid>https://orcid.org/0000-0002-7966-0663</orcidid><orcidid>https://orcid.org/0000-0002-2010-6107</orcidid><orcidid>https://orcid.org/0000-0003-0478-5020</orcidid></search><sort><creationdate>19991201</creationdate><title>A novel model to study the dorsolateral migration of melanoblasts</title><author>Beauvais-Jouneau, Alice ; Pla, Patrick ; Bernex, Florence ; Dufour, Sylvie ; Salamero, Jean ; Fässler, Reinhard ; Panthier, Jean-Jacques ; Thiery, Jean Paul ; Larue, Lionel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-825e3fecb033fd98b05f6156aad8186e0c38d322efed6e5a91d4197214d361783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Binding Sites</topic><topic>Biomarkers</topic><topic>Cell Line</topic><topic>Cell Movement - physiology</topic><topic>Chick Embryo</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Embryo</topic><topic>Embryonic Induction</topic><topic>Embryonic stem cell</topic><topic>Endothelin-3 - genetics</topic><topic>Fibronectins - metabolism</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Integrin beta1 - genetics</topic><topic>Integrin beta1 - metabolism</topic><topic>Intramolecular Oxidoreductases - genetics</topic><topic>Life Sciences</topic><topic>Melanocyte</topic><topic>Melanocytes - physiology</topic><topic>Membrane Glycoproteins</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Microphthalmia-Associated Transcription Factor</topic><topic>Monophenol Monooxygenase - genetics</topic><topic>Mutation</topic><topic>Nervous System - cytology</topic><topic>Nervous System - embryology</topic><topic>Neural crest</topic><topic>Oxidoreductases</topic><topic>Pigmented cell</topic><topic>Proteins - genetics</topic><topic>Proto-Oncogene Proteins c-kit - genetics</topic><topic>Receptor, Endothelin B</topic><topic>Receptors, Endothelin - genetics</topic><topic>Snail Family Transcription Factors</topic><topic>Stem Cell Transplantation</topic><topic>Transcription Factors - genetics</topic><topic>Xenograft</topic><topic>β1 integrin</topic><toplevel>online_resources</toplevel><creatorcontrib>Beauvais-Jouneau, Alice</creatorcontrib><creatorcontrib>Pla, Patrick</creatorcontrib><creatorcontrib>Bernex, Florence</creatorcontrib><creatorcontrib>Dufour, Sylvie</creatorcontrib><creatorcontrib>Salamero, Jean</creatorcontrib><creatorcontrib>Fässler, Reinhard</creatorcontrib><creatorcontrib>Panthier, Jean-Jacques</creatorcontrib><creatorcontrib>Thiery, Jean Paul</creatorcontrib><creatorcontrib>Larue, Lionel</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Mechanisms of development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Beauvais-Jouneau, Alice</au><au>Pla, Patrick</au><au>Bernex, Florence</au><au>Dufour, Sylvie</au><au>Salamero, Jean</au><au>Fässler, Reinhard</au><au>Panthier, Jean-Jacques</au><au>Thiery, Jean Paul</au><au>Larue, Lionel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel model to study the dorsolateral migration of melanoblasts</atitle><jtitle>Mechanisms of development</jtitle><addtitle>Mech Dev</addtitle><date>1999-12-01</date><risdate>1999</risdate><volume>89</volume><issue>1</issue><spage>3</spage><epage>14</epage><pages>3-14</pages><issn>0925-4773</issn><eissn>1872-6356</eissn><abstract>Melanocytes derived from pluripotent neural crest cells migrate initially in the dorsolateral pathway between the ectoderm and dermomyotome. To understand the role of specific proteins involved in this cell migration, we looked for a cellular model that mimics the in vivo behavior of melanoblasts, and that allows functional studies of their migration. We report here that wild-type embryonic stem (ES) cells are able to follow the ventral and dorsolateral neural crest pathways after being grafted into chicken embryos. By contrast, a mutant ES cell line deficient for
β1 integrin subunits, proteins involved in cell–extracellular interactions, had a severely impaired migratory behavior. Interestingly, ES cells deficient for Kit, the tyrosine kinase receptor for the stem cell factor (SCF), behaved similarly to wild-type ES cells. Thus, grafting mouse ES cells into chicken embryos provides a new cellular system that allows both in vitro and in vivo studies of the molecular mechanisms controlling dorsolateral migration.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>10559475</pmid><doi>10.1016/S0925-4773(99)00191-4</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-0145-6937</orcidid><orcidid>https://orcid.org/0000-0002-5957-2340</orcidid><orcidid>https://orcid.org/0000-0003-0366-4315</orcidid><orcidid>https://orcid.org/0000-0002-8646-4935</orcidid><orcidid>https://orcid.org/0000-0002-7966-0663</orcidid><orcidid>https://orcid.org/0000-0002-2010-6107</orcidid><orcidid>https://orcid.org/0000-0003-0478-5020</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Mechanisms of development, 1999-12, Vol.89 (1), p.3-14 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals beta-Galactosidase - genetics beta-Galactosidase - metabolism Binding Sites Biomarkers Cell Line Cell Movement - physiology Chick Embryo DNA-Binding Proteins - genetics Embryo Embryonic Induction Embryonic stem cell Endothelin-3 - genetics Fibronectins - metabolism Fluorescent Dyes - metabolism Gene Expression Regulation, Developmental Integrin beta1 - genetics Integrin beta1 - metabolism Intramolecular Oxidoreductases - genetics Life Sciences Melanocyte Melanocytes - physiology Membrane Glycoproteins Mice Mice, Mutant Strains Microphthalmia-Associated Transcription Factor Monophenol Monooxygenase - genetics Mutation Nervous System - cytology Nervous System - embryology Neural crest Oxidoreductases Pigmented cell Proteins - genetics Proto-Oncogene Proteins c-kit - genetics Receptor, Endothelin B Receptors, Endothelin - genetics Snail Family Transcription Factors Stem Cell Transplantation Transcription Factors - genetics Xenograft β1 integrin |
| title | A novel model to study the dorsolateral migration of melanoblasts |
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