TAF7 (TAFII55) Plays a Role in the Transcription Activation by c-Jun
c-Jun is a member of the AP-1 family of transcription factors regulating expression of specific target genes in a variety of cellular processes including proliferation, stress response, and tumorigenicity. In the present study we have analyzed the mechanism of c-Jun function as a transactivator with...
Gespeichert in:
Veröffentlicht in: | The Journal of biological chemistry 2003-04, Vol.278 (24), p.21510-21516 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 21516 |
---|---|
container_issue | 24 |
container_start_page | 21510 |
container_title | The Journal of biological chemistry |
container_volume | 278 |
creator | Munz, Christine Psichari, Eleni Mandilis, Dimitris Lavigne, Anne-Claire Spiliotaki, Maria Oehler, Thomas Davidson, Irwin Tora, Làszlo Angel, Peter Pintzas, Alexander |
description | c-Jun is a member of the AP-1 family of transcription factors regulating expression of specific target genes in a variety of cellular processes including proliferation, stress response, and tumorigenicity. In the present study we have analyzed the mechanism of c-Jun function as a transactivator with respect to members of the basal transcription machinery, TATA-binding proteinassociated factors (TAFs). We show that one member of the family, human TAF7 (formerly TAF II 55), physically interacts with c-Jun through two independent interaction domains, within the N-and C-terminal part of c-Jun. Interaction in vitro correlates with enhanced transactivation function of c-Jun in HEK293 and COS cells in the presence of increasing amounts of TAF7. TAF7 interacts preferentially with DNA-bound phosphorylated c-Jun, suggesting that TAF7 represents a novel c-Jun co-activator mediating activation of AP-1 target genes in response to extracellular signals. |
doi_str_mv | 10.1074/jbc.m212764200 |
format | Article |
fullrecord | <record><control><sourceid>hal</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_03108309v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_HAL_hal_03108309v1</sourcerecordid><originalsourceid>FETCH-hal_primary_oai_HAL_hal_03108309v13</originalsourceid><addsrcrecordid>eNqVjr1ug0AQhFdWIoOTtKm3jAvs3YPjTIkSW2C5sCyKdOiMiDjEj8URS7x97CgvkClmPo2mGIBXphWTCtb1uVi1goUKA0E0A5dp43u-5M8HcIkEe5GQGwcW1tZ0UxDxHBwWoQojqVz4yOKdwrebp6mUSzw2erKo8dQ3JZoOx6rEbNCdLQZzGU3fYVyM5qp_8Txh4e2_u2d4_NKNLV_-8gmWu232nniVbvLLYFo9THmvTZ7Eh_zekX-_SdGV_f9sfwC1-EPN</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>TAF7 (TAFII55) Plays a Role in the Transcription Activation by c-Jun</title><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Munz, Christine ; Psichari, Eleni ; Mandilis, Dimitris ; Lavigne, Anne-Claire ; Spiliotaki, Maria ; Oehler, Thomas ; Davidson, Irwin ; Tora, Làszlo ; Angel, Peter ; Pintzas, Alexander</creator><creatorcontrib>Munz, Christine ; Psichari, Eleni ; Mandilis, Dimitris ; Lavigne, Anne-Claire ; Spiliotaki, Maria ; Oehler, Thomas ; Davidson, Irwin ; Tora, Làszlo ; Angel, Peter ; Pintzas, Alexander</creatorcontrib><description>c-Jun is a member of the AP-1 family of transcription factors regulating expression of specific target genes in a variety of cellular processes including proliferation, stress response, and tumorigenicity. In the present study we have analyzed the mechanism of c-Jun function as a transactivator with respect to members of the basal transcription machinery, TATA-binding proteinassociated factors (TAFs). We show that one member of the family, human TAF7 (formerly TAF II 55), physically interacts with c-Jun through two independent interaction domains, within the N-and C-terminal part of c-Jun. Interaction in vitro correlates with enhanced transactivation function of c-Jun in HEK293 and COS cells in the presence of increasing amounts of TAF7. TAF7 interacts preferentially with DNA-bound phosphorylated c-Jun, suggesting that TAF7 represents a novel c-Jun co-activator mediating activation of AP-1 target genes in response to extracellular signals.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.m212764200</identifier><identifier>PMID: 12676957</identifier><language>eng</language><publisher>American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; Blotting, Western ; Cell Line ; COS Cells ; Glutathione Transferase ; Humans ; JNK Mitogen-Activated Protein Kinases ; Life Sciences ; Luciferases ; Mitogen-Activated Protein Kinases ; Oligonucleotides ; Phosphorylation ; Plasmids ; Precipitin Tests ; Protein Binding ; Protein Structure, Tertiary ; Recombinant Proteins ; TATA-Binding Protein Associated Factors ; Transcription Factor AP-1 ; Transcription Factor TFIID ; Transcription, Genetic ; Transcriptional Activation ; Transfection</subject><ispartof>The Journal of biological chemistry, 2003-04, Vol.278 (24), p.21510-21516</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-7398-2250 ; 0000-0001-9389-7029 ; 0000-0001-5533-1171 ; 0000-0001-9389-7029 ; 0000-0001-5533-1171 ; 0000-0001-7398-2250</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27903,27904</link.rule.ids><backlink>$$Uhttps://hal.science/hal-03108309$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Munz, Christine</creatorcontrib><creatorcontrib>Psichari, Eleni</creatorcontrib><creatorcontrib>Mandilis, Dimitris</creatorcontrib><creatorcontrib>Lavigne, Anne-Claire</creatorcontrib><creatorcontrib>Spiliotaki, Maria</creatorcontrib><creatorcontrib>Oehler, Thomas</creatorcontrib><creatorcontrib>Davidson, Irwin</creatorcontrib><creatorcontrib>Tora, Làszlo</creatorcontrib><creatorcontrib>Angel, Peter</creatorcontrib><creatorcontrib>Pintzas, Alexander</creatorcontrib><title>TAF7 (TAFII55) Plays a Role in the Transcription Activation by c-Jun</title><title>The Journal of biological chemistry</title><description>c-Jun is a member of the AP-1 family of transcription factors regulating expression of specific target genes in a variety of cellular processes including proliferation, stress response, and tumorigenicity. In the present study we have analyzed the mechanism of c-Jun function as a transactivator with respect to members of the basal transcription machinery, TATA-binding proteinassociated factors (TAFs). We show that one member of the family, human TAF7 (formerly TAF II 55), physically interacts with c-Jun through two independent interaction domains, within the N-and C-terminal part of c-Jun. Interaction in vitro correlates with enhanced transactivation function of c-Jun in HEK293 and COS cells in the presence of increasing amounts of TAF7. TAF7 interacts preferentially with DNA-bound phosphorylated c-Jun, suggesting that TAF7 represents a novel c-Jun co-activator mediating activation of AP-1 target genes in response to extracellular signals.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>COS Cells</subject><subject>Glutathione Transferase</subject><subject>Humans</subject><subject>JNK Mitogen-Activated Protein Kinases</subject><subject>Life Sciences</subject><subject>Luciferases</subject><subject>Mitogen-Activated Protein Kinases</subject><subject>Oligonucleotides</subject><subject>Phosphorylation</subject><subject>Plasmids</subject><subject>Precipitin Tests</subject><subject>Protein Binding</subject><subject>Protein Structure, Tertiary</subject><subject>Recombinant Proteins</subject><subject>TATA-Binding Protein Associated Factors</subject><subject>Transcription Factor AP-1</subject><subject>Transcription Factor TFIID</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><subject>Transfection</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqVjr1ug0AQhFdWIoOTtKm3jAvs3YPjTIkSW2C5sCyKdOiMiDjEj8URS7x97CgvkClmPo2mGIBXphWTCtb1uVi1goUKA0E0A5dp43u-5M8HcIkEe5GQGwcW1tZ0UxDxHBwWoQojqVz4yOKdwrebp6mUSzw2erKo8dQ3JZoOx6rEbNCdLQZzGU3fYVyM5qp_8Txh4e2_u2d4_NKNLV_-8gmWu232nniVbvLLYFo9THmvTZ7Eh_zekX-_SdGV_f9sfwC1-EPN</recordid><startdate>20030403</startdate><enddate>20030403</enddate><creator>Munz, Christine</creator><creator>Psichari, Eleni</creator><creator>Mandilis, Dimitris</creator><creator>Lavigne, Anne-Claire</creator><creator>Spiliotaki, Maria</creator><creator>Oehler, Thomas</creator><creator>Davidson, Irwin</creator><creator>Tora, Làszlo</creator><creator>Angel, Peter</creator><creator>Pintzas, Alexander</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0001-7398-2250</orcidid><orcidid>https://orcid.org/0000-0001-9389-7029</orcidid><orcidid>https://orcid.org/0000-0001-5533-1171</orcidid><orcidid>https://orcid.org/0000-0001-9389-7029</orcidid><orcidid>https://orcid.org/0000-0001-5533-1171</orcidid><orcidid>https://orcid.org/0000-0001-7398-2250</orcidid></search><sort><creationdate>20030403</creationdate><title>TAF7 (TAFII55) Plays a Role in the Transcription Activation by c-Jun</title><author>Munz, Christine ; Psichari, Eleni ; Mandilis, Dimitris ; Lavigne, Anne-Claire ; Spiliotaki, Maria ; Oehler, Thomas ; Davidson, Irwin ; Tora, Làszlo ; Angel, Peter ; Pintzas, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-hal_primary_oai_HAL_hal_03108309v13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>COS Cells</topic><topic>Glutathione Transferase</topic><topic>Humans</topic><topic>JNK Mitogen-Activated Protein Kinases</topic><topic>Life Sciences</topic><topic>Luciferases</topic><topic>Mitogen-Activated Protein Kinases</topic><topic>Oligonucleotides</topic><topic>Phosphorylation</topic><topic>Plasmids</topic><topic>Precipitin Tests</topic><topic>Protein Binding</topic><topic>Protein Structure, Tertiary</topic><topic>Recombinant Proteins</topic><topic>TATA-Binding Protein Associated Factors</topic><topic>Transcription Factor AP-1</topic><topic>Transcription Factor TFIID</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Munz, Christine</creatorcontrib><creatorcontrib>Psichari, Eleni</creatorcontrib><creatorcontrib>Mandilis, Dimitris</creatorcontrib><creatorcontrib>Lavigne, Anne-Claire</creatorcontrib><creatorcontrib>Spiliotaki, Maria</creatorcontrib><creatorcontrib>Oehler, Thomas</creatorcontrib><creatorcontrib>Davidson, Irwin</creatorcontrib><creatorcontrib>Tora, Làszlo</creatorcontrib><creatorcontrib>Angel, Peter</creatorcontrib><creatorcontrib>Pintzas, Alexander</creatorcontrib><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Munz, Christine</au><au>Psichari, Eleni</au><au>Mandilis, Dimitris</au><au>Lavigne, Anne-Claire</au><au>Spiliotaki, Maria</au><au>Oehler, Thomas</au><au>Davidson, Irwin</au><au>Tora, Làszlo</au><au>Angel, Peter</au><au>Pintzas, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TAF7 (TAFII55) Plays a Role in the Transcription Activation by c-Jun</atitle><jtitle>The Journal of biological chemistry</jtitle><date>2003-04-03</date><risdate>2003</risdate><volume>278</volume><issue>24</issue><spage>21510</spage><epage>21516</epage><pages>21510-21516</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>c-Jun is a member of the AP-1 family of transcription factors regulating expression of specific target genes in a variety of cellular processes including proliferation, stress response, and tumorigenicity. In the present study we have analyzed the mechanism of c-Jun function as a transactivator with respect to members of the basal transcription machinery, TATA-binding proteinassociated factors (TAFs). We show that one member of the family, human TAF7 (formerly TAF II 55), physically interacts with c-Jun through two independent interaction domains, within the N-and C-terminal part of c-Jun. Interaction in vitro correlates with enhanced transactivation function of c-Jun in HEK293 and COS cells in the presence of increasing amounts of TAF7. TAF7 interacts preferentially with DNA-bound phosphorylated c-Jun, suggesting that TAF7 represents a novel c-Jun co-activator mediating activation of AP-1 target genes in response to extracellular signals.</abstract><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>12676957</pmid><doi>10.1074/jbc.m212764200</doi><orcidid>https://orcid.org/0000-0001-7398-2250</orcidid><orcidid>https://orcid.org/0000-0001-9389-7029</orcidid><orcidid>https://orcid.org/0000-0001-5533-1171</orcidid><orcidid>https://orcid.org/0000-0001-9389-7029</orcidid><orcidid>https://orcid.org/0000-0001-5533-1171</orcidid><orcidid>https://orcid.org/0000-0001-7398-2250</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0021-9258 |
ispartof | The Journal of biological chemistry, 2003-04, Vol.278 (24), p.21510-21516 |
issn | 0021-9258 1083-351X |
language | eng |
recordid | cdi_hal_primary_oai_HAL_hal_03108309v1 |
source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | Animals Blotting, Western Cell Line COS Cells Glutathione Transferase Humans JNK Mitogen-Activated Protein Kinases Life Sciences Luciferases Mitogen-Activated Protein Kinases Oligonucleotides Phosphorylation Plasmids Precipitin Tests Protein Binding Protein Structure, Tertiary Recombinant Proteins TATA-Binding Protein Associated Factors Transcription Factor AP-1 Transcription Factor TFIID Transcription, Genetic Transcriptional Activation Transfection |
title | TAF7 (TAFII55) Plays a Role in the Transcription Activation by c-Jun |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T23%3A29%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-hal&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=TAF7%20(TAFII55)%20Plays%20a%20Role%20in%20the%20Transcription%20Activation%20by%20c-Jun&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Munz,%20Christine&rft.date=2003-04-03&rft.volume=278&rft.issue=24&rft.spage=21510&rft.epage=21516&rft.pages=21510-21516&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1074/jbc.m212764200&rft_dat=%3Chal%3Eoai_HAL_hal_03108309v1%3C/hal%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/12676957&rfr_iscdi=true |