An acetyltransferase assay for CREB-binding protein based on reverse phase–ultra-fast liquid chromatography of fluorescent histone H3 peptides

CREB-binding protein (CBP) is a lysine acetyltransferase that regulates transcription by acetylating histone and non-histone substrates. Defects in CBP activity are associated with hematologic malignancies, neurodisorders, and congenital malformations. Sensitive and quantitative enzymatic assays are...

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Veröffentlicht in:	Analytical biochemistry 2015-10, Vol.486, p.35-37
Hauptverfasser:	Duval, Romain, Fritsch, Lauriane, Bui, Linh-Chi, Berthelet, Jérémy, Guidez, Fabien, Mathieu, Cécile, Dupret, Jean-Marie, Chomienne, Christine, Ait-Si-Ali, Slimane, Rodrigues-Lima, Fernando
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography, High Pressure Liquid Chromatography, Reverse-Phase CREB-binding protein CREB-Binding Protein - metabolism Enzyme Assays - methods Fluorescent Dyes - chemistry Fluorescent Dyes - metabolism Fluorescent peptide Histone acetyltransferase Histone methyltransferases Histones - chemistry HPLC Humans Kinetics Life Sciences Molecular Sequence Data Peptide Fragments - chemistry Peptide Fragments - metabolism SETD2
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