Protection evaluation against Chlamydophila abortus challenge by DNA vaccination with a dnaK-encoding plasmid in pregnant and non-pregnant mice
Mice were intramuscularly immunized with a dnaK-encoding DNA plasmid. The protective effect of DNA immunization against Chlamydophila abortus infection was studied in pregnant and non-pregnant mice models. In non-pregnant mice, the dnaK vaccine induced a specific humoral response with the predominan...
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| Veröffentlicht in: | Veterinary research (Paris) 2002-05, Vol.33 (3), p.313-326 |
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| creator | HECHARD, Céline GREPINET, Olivier RODOLAKIS, Annie |
| description | Mice were intramuscularly immunized with a dnaK-encoding DNA plasmid. The protective effect of DNA immunization against Chlamydophila abortus infection was studied in pregnant and non-pregnant mice models. In non-pregnant mice, the dnaK vaccine induced a specific humoral response with the predominant IgG2a isotype, which failed to have in vitro neutralizing properties. No delayed-type hypersensitivity reaction was observed and the spleens of dnaK vaccinated-mice were not protected against C. abortus challenge. In pregnant mice, the dnaK vaccine induced a non-specific partial protection from abortion. This may be due to the immunogenic properties of the CpG motifs of bacterial DNA present in the vaccinal plasmid backbone. Nevertheless, spleens of dnaK vaccinated-pregnant mice were not protected. |
| doi_str_mv | 10.1051/vetres:2002019 |
| format | Article |
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The protective effect of DNA immunization against Chlamydophila abortus infection was studied in pregnant and non-pregnant mice models. In non-pregnant mice, the dnaK vaccine induced a specific humoral response with the predominant IgG2a isotype, which failed to have in vitro neutralizing properties. No delayed-type hypersensitivity reaction was observed and the spleens of dnaK vaccinated-mice were not protected against C. abortus challenge. In pregnant mice, the dnaK vaccine induced a non-specific partial protection from abortion. This may be due to the immunogenic properties of the CpG motifs of bacterial DNA present in the vaccinal plasmid backbone. Nevertheless, spleens of dnaK vaccinated-pregnant mice were not protected.</description><identifier>ISSN: 0928-4249</identifier><identifier>EISSN: 1297-9716</identifier><identifier>DOI: 10.1051/vetres:2002019</identifier><identifier>PMID: 12056482</identifier><language>eng</language><publisher>Les Ulis: EDP Sciences</publisher><subject>Animals ; Antibodies, Bacterial - biosynthesis ; Bacteriology ; Biological and medical sciences ; Chlamydophila - genetics ; Chlamydophila - immunology ; Chlamydophila Infections - prevention & control ; Chlamydophila Infections - veterinary ; Disease Models, Animal ; Escherichia coli Proteins ; Female ; Fundamental and applied biological sciences. Psychology ; HSP70 Heat-Shock Proteins - genetics ; HSP70 Heat-Shock Proteins - immunology ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - blood ; Injections, Intramuscular - veterinary ; Life Sciences ; Mice ; Microbiology ; Microbiology and Parasitology ; Plasmids ; Pregnancy ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Spleen - immunology ; Spleen - microbiology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology</subject><ispartof>Veterinary research (Paris), 2002-05, Vol.33 (3), p.313-326</ispartof><rights>2002 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-2d0642d52b6fc7fa3b34b4b0f0658b7eada335ad97c037672a0bb06c3a7943513</citedby><orcidid>0000-0001-5085-474X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13664730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12056482$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-03044507$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>HECHARD, Céline</creatorcontrib><creatorcontrib>GREPINET, Olivier</creatorcontrib><creatorcontrib>RODOLAKIS, Annie</creatorcontrib><title>Protection evaluation against Chlamydophila abortus challenge by DNA vaccination with a dnaK-encoding plasmid in pregnant and non-pregnant mice</title><title>Veterinary research (Paris)</title><addtitle>Vet Res</addtitle><description>Mice were intramuscularly immunized with a dnaK-encoding DNA plasmid. The protective effect of DNA immunization against Chlamydophila abortus infection was studied in pregnant and non-pregnant mice models. In non-pregnant mice, the dnaK vaccine induced a specific humoral response with the predominant IgG2a isotype, which failed to have in vitro neutralizing properties. No delayed-type hypersensitivity reaction was observed and the spleens of dnaK vaccinated-mice were not protected against C. abortus challenge. In pregnant mice, the dnaK vaccine induced a non-specific partial protection from abortion. This may be due to the immunogenic properties of the CpG motifs of bacterial DNA present in the vaccinal plasmid backbone. Nevertheless, spleens of dnaK vaccinated-pregnant mice were not protected.</description><subject>Animals</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chlamydophila - genetics</subject><subject>Chlamydophila - immunology</subject><subject>Chlamydophila Infections - prevention & control</subject><subject>Chlamydophila Infections - veterinary</subject><subject>Disease Models, Animal</subject><subject>Escherichia coli Proteins</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HSP70 Heat-Shock Proteins - genetics</subject><subject>HSP70 Heat-Shock Proteins - immunology</subject><subject>Immunoglobulin G - biosynthesis</subject><subject>Immunoglobulin G - blood</subject><subject>Injections, Intramuscular - veterinary</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Plasmids</subject><subject>Pregnancy</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Spleen - immunology</subject><subject>Spleen - microbiology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - immunology</subject><issn>0928-4249</issn><issn>1297-9716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UuP0zAQB_AIgdiycOWIfAGJQ5bxI3bCrSqPRVTAAc7R-JHWKLGDnRT1U_CVaUm1e-Q01ug3o7H-RfGcwg2Fir45uCm5_JYBMKDNg2JFWaPKRlH5sFhBw-pSMNFcFU9y_glAJa_E4-KKMqikqNmq-PMtxcmZycdA3AH7Gf89cYc-5Ils9j0ORxvHve-RoI5pmjMxe-x7F3aO6CN592VNDmiMD8vobz_tCRIb8HPpgonWhx0Ze8yDt8QHMia3CxgmgsGSEEN51xi8cU-LRx322T271Ovix4f33ze35fbrx0-b9bY0gsmpZBakYLZiWnZGdcg1F1po6EBWtVYOLXJeoW2UAa6kYghagzQcVSN4Rfl18XrZe_pKOyY_YDq2EX17u9625x5wEKICdTjbV4sdU_w1uzy1g8_G9T0GF-fcKloDb1TzX0hrIWpVVyd4s0CTYs7JdXcnUGjPubZLru0l19PAi8vmWQ_O3vNLkCfw8gIwG-y7hMH4fO-4lEJx4H8BhuGtvA</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>HECHARD, Céline</creator><creator>GREPINET, Olivier</creator><creator>RODOLAKIS, Annie</creator><general>EDP Sciences</general><general>BioMed Central</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5085-474X</orcidid></search><sort><creationdate>20020501</creationdate><title>Protection evaluation against Chlamydophila abortus challenge by DNA vaccination with a dnaK-encoding plasmid in pregnant and non-pregnant mice</title><author>HECHARD, Céline ; GREPINET, Olivier ; RODOLAKIS, Annie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-2d0642d52b6fc7fa3b34b4b0f0658b7eada335ad97c037672a0bb06c3a7943513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - biosynthesis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chlamydophila - genetics</topic><topic>Chlamydophila - immunology</topic><topic>Chlamydophila Infections - prevention & control</topic><topic>Chlamydophila Infections - veterinary</topic><topic>Disease Models, Animal</topic><topic>Escherichia coli Proteins</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HSP70 Heat-Shock Proteins - genetics</topic><topic>HSP70 Heat-Shock Proteins - immunology</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin G - blood</topic><topic>Injections, Intramuscular - veterinary</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Plasmids</topic><topic>Pregnancy</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Spleen - immunology</topic><topic>Spleen - microbiology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HECHARD, Céline</creatorcontrib><creatorcontrib>GREPINET, Olivier</creatorcontrib><creatorcontrib>RODOLAKIS, Annie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Veterinary research (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HECHARD, Céline</au><au>GREPINET, Olivier</au><au>RODOLAKIS, Annie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protection evaluation against Chlamydophila abortus challenge by DNA vaccination with a dnaK-encoding plasmid in pregnant and non-pregnant mice</atitle><jtitle>Veterinary research (Paris)</jtitle><addtitle>Vet Res</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>33</volume><issue>3</issue><spage>313</spage><epage>326</epage><pages>313-326</pages><issn>0928-4249</issn><eissn>1297-9716</eissn><abstract>Mice were intramuscularly immunized with a dnaK-encoding DNA plasmid. The protective effect of DNA immunization against Chlamydophila abortus infection was studied in pregnant and non-pregnant mice models. In non-pregnant mice, the dnaK vaccine induced a specific humoral response with the predominant IgG2a isotype, which failed to have in vitro neutralizing properties. No delayed-type hypersensitivity reaction was observed and the spleens of dnaK vaccinated-mice were not protected against C. abortus challenge. In pregnant mice, the dnaK vaccine induced a non-specific partial protection from abortion. This may be due to the immunogenic properties of the CpG motifs of bacterial DNA present in the vaccinal plasmid backbone. Nevertheless, spleens of dnaK vaccinated-pregnant mice were not protected.</abstract><cop>Les Ulis</cop><pub>EDP Sciences</pub><pmid>12056482</pmid><doi>10.1051/vetres:2002019</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-5085-474X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibodies, Bacterial - biosynthesis Bacteriology Biological and medical sciences Chlamydophila - genetics Chlamydophila - immunology Chlamydophila Infections - prevention & control Chlamydophila Infections - veterinary Disease Models, Animal Escherichia coli Proteins Female Fundamental and applied biological sciences. Psychology HSP70 Heat-Shock Proteins - genetics HSP70 Heat-Shock Proteins - immunology Immunoglobulin G - biosynthesis Immunoglobulin G - blood Injections, Intramuscular - veterinary Life Sciences Mice Microbiology Microbiology and Parasitology Plasmids Pregnancy Recombinant Proteins - genetics Recombinant Proteins - immunology Spleen - immunology Spleen - microbiology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA - administration & dosage Vaccines, DNA - immunology |
| title | Protection evaluation against Chlamydophila abortus challenge by DNA vaccination with a dnaK-encoding plasmid in pregnant and non-pregnant mice |
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