Sperm-induced currents at fertilization in sea urchin eggs injected with EGTA and neomycin
Membrane currents were measured in single voltage-clamped sea urchin eggs ( Lytechinus pictus and Lytechinus variegatus) that were injected with either EGTA or neomycin and inseminated. Although egg activation and the fertilization calcium wave were prevented by injection of either of these compound...
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| Veröffentlicht in: | Developmental biology 1992-06, Vol.151 (2), p.552-563 |
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| creator | Swann, Karl McCulloh, David H. McDougall, Alex Chambers, Edward L. Whitaker, Michael |
| description | Membrane currents were measured in single voltage-clamped sea urchin eggs (
Lytechinus pictus and
Lytechinus variegatus) that were injected with either EGTA or neomycin and inseminated. Although egg activation and the fertilization calcium wave were prevented by injection of either of these compounds, sperm attached and still elicited inward currents. Sperm-induced currents in EGTA-injected eggs had an abrupt onset, quickly reached a maximum, and then slowly declined in amplitude. Sperm incorporation occurred readily in EGTA-injected eggs. Similar results were obtained with another calcium chelator, BAPTA. In neomycin-injected eggs, sperm-induced currents generally had an abrupt onset and, in contrast to EGTA-injected eggs, the currents usually cut off rapidly. Sperm failed to enter the neomycin-injected eggs and the duration of sperm-induced currents in neomycin-injected eggs was markedly dependent upon the voltage-clamp holding potential, with shorter duration currents occurring at −70 than at −20 mV. The lability of the initial interaction between sperm and egg at negative holding potentials may explain why activation often fails when the egg membrane is voltage clamped at these potentials (Lynn
et al., Dev. Biol.
128, 305–323, 1988). |
| doi_str_mv | 10.1016/0012-1606(92)90193-K |
| format | Article |
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Lytechinus pictus and
Lytechinus variegatus) that were injected with either EGTA or neomycin and inseminated. Although egg activation and the fertilization calcium wave were prevented by injection of either of these compounds, sperm attached and still elicited inward currents. Sperm-induced currents in EGTA-injected eggs had an abrupt onset, quickly reached a maximum, and then slowly declined in amplitude. Sperm incorporation occurred readily in EGTA-injected eggs. Similar results were obtained with another calcium chelator, BAPTA. In neomycin-injected eggs, sperm-induced currents generally had an abrupt onset and, in contrast to EGTA-injected eggs, the currents usually cut off rapidly. Sperm failed to enter the neomycin-injected eggs and the duration of sperm-induced currents in neomycin-injected eggs was markedly dependent upon the voltage-clamp holding potential, with shorter duration currents occurring at −70 than at −20 mV. The lability of the initial interaction between sperm and egg at negative holding potentials may explain why activation often fails when the egg membrane is voltage clamped at these potentials (Lynn
et al., Dev. Biol.
128, 305–323, 1988).</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(92)90193-K</identifier><identifier>PMID: 1318235</identifier><identifier>CODEN: DEBIAO</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; biological fertilization ; Calcium Channels - drug effects ; Calcium Channels - physiology ; cell membranes ; Cellular Biology ; Echinodermata ; eggs ; EGTA ; Egtazic Acid - pharmacology ; Electrophysiology ; Female ; fertilization ; Fertilization - drug effects ; Fundamental and applied biological sciences. Psychology ; injection ; Invertebrates ; Ion Channel Gating ; Life Sciences ; Lytechinus ; Lytechinus pictus ; Lytechinus variegatus ; Male ; Marine ; membrane currents ; Membrane Potentials - drug effects ; Microinjections ; neomycin ; Neomycin - pharmacology ; Reproductive Biology ; Sea Urchins - physiology ; sperm ; Sperm-Ovum Interactions - drug effects</subject><ispartof>Developmental biology, 1992-06, Vol.151 (2), p.552-563</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c548t-2cef3fc1b0f65ae839b43e6afcbfb2dabb9c0b2942f43acc001bc539f99821603</citedby><cites>FETCH-LOGICAL-c548t-2cef3fc1b0f65ae839b43e6afcbfb2dabb9c0b2942f43acc001bc539f99821603</cites><orcidid>0000-0003-0324-3836</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0012-1606(92)90193-K$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5399791$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1318235$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-03025613$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Swann, Karl</creatorcontrib><creatorcontrib>McCulloh, David H.</creatorcontrib><creatorcontrib>McDougall, Alex</creatorcontrib><creatorcontrib>Chambers, Edward L.</creatorcontrib><creatorcontrib>Whitaker, Michael</creatorcontrib><title>Sperm-induced currents at fertilization in sea urchin eggs injected with EGTA and neomycin</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>Membrane currents were measured in single voltage-clamped sea urchin eggs (
Lytechinus pictus and
Lytechinus variegatus) that were injected with either EGTA or neomycin and inseminated. Although egg activation and the fertilization calcium wave were prevented by injection of either of these compounds, sperm attached and still elicited inward currents. Sperm-induced currents in EGTA-injected eggs had an abrupt onset, quickly reached a maximum, and then slowly declined in amplitude. Sperm incorporation occurred readily in EGTA-injected eggs. Similar results were obtained with another calcium chelator, BAPTA. In neomycin-injected eggs, sperm-induced currents generally had an abrupt onset and, in contrast to EGTA-injected eggs, the currents usually cut off rapidly. Sperm failed to enter the neomycin-injected eggs and the duration of sperm-induced currents in neomycin-injected eggs was markedly dependent upon the voltage-clamp holding potential, with shorter duration currents occurring at −70 than at −20 mV. The lability of the initial interaction between sperm and egg at negative holding potentials may explain why activation often fails when the egg membrane is voltage clamped at these potentials (Lynn
et al., Dev. Biol.
128, 305–323, 1988).</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>biological fertilization</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - physiology</subject><subject>cell membranes</subject><subject>Cellular Biology</subject><subject>Echinodermata</subject><subject>eggs</subject><subject>EGTA</subject><subject>Egtazic Acid - pharmacology</subject><subject>Electrophysiology</subject><subject>Female</subject><subject>fertilization</subject><subject>Fertilization - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>injection</subject><subject>Invertebrates</subject><subject>Ion Channel Gating</subject><subject>Life Sciences</subject><subject>Lytechinus</subject><subject>Lytechinus pictus</subject><subject>Lytechinus variegatus</subject><subject>Male</subject><subject>Marine</subject><subject>membrane currents</subject><subject>Membrane Potentials - drug effects</subject><subject>Microinjections</subject><subject>neomycin</subject><subject>Neomycin - pharmacology</subject><subject>Reproductive Biology</subject><subject>Sea Urchins - physiology</subject><subject>sperm</subject><subject>Sperm-Ovum Interactions - drug effects</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKkvhH4DkA0L0EPBHko0vSKuqtKgrcaBIiItlT8ZdV4mz2ElR-fU4ZLXc4GTL88xoXj-EvOTsHWe8fs8YFwWvWf1WiTPFuJLF9SOy4kxVRVWX3x6T1RF5Sp6ldMcYk00jT8gJl7wRslqR71_2GPvCh3YCbClMMWIYEzUjdRhH3_lfZvRDoD7QhIZOEXb5ire3KT_dIYy566cfd_Ti8mZDTWhpwKF_AB-ekyfOdAlfHM5T8vXjxc35VbH9fPnpfLMtoCqbsRCATjrglrm6MthIZUuJtXFgnRWtsVYBs0KVwpXSAORMFiqpnFKNyNnkKTlb5u5Mp_fR9yY-6MF4fbXZ6vmNSSaqmst7ntk3C7uPw48J06h7nwC7zuStp6TXQjXNumT_BXktuViXIoPlAkIcUorojitwpmdPepagZwlaCf3Hk77Oba8O8yfbY_u3aRGT668PdZPAdC6aAD4dsZxfrdWc58OCYf7ge49RJ_AYskofsxvdDv7fe_wG8wCuJw</recordid><startdate>19920601</startdate><enddate>19920601</enddate><creator>Swann, Karl</creator><creator>McCulloh, David H.</creator><creator>McDougall, Alex</creator><creator>Chambers, Edward L.</creator><creator>Whitaker, Michael</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0003-0324-3836</orcidid></search><sort><creationdate>19920601</creationdate><title>Sperm-induced currents at fertilization in sea urchin eggs injected with EGTA and neomycin</title><author>Swann, Karl ; McCulloh, David H. ; McDougall, Alex ; Chambers, Edward L. ; Whitaker, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c548t-2cef3fc1b0f65ae839b43e6afcbfb2dabb9c0b2942f43acc001bc539f99821603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>biological fertilization</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - physiology</topic><topic>cell membranes</topic><topic>Cellular Biology</topic><topic>Echinodermata</topic><topic>eggs</topic><topic>EGTA</topic><topic>Egtazic Acid - pharmacology</topic><topic>Electrophysiology</topic><topic>Female</topic><topic>fertilization</topic><topic>Fertilization - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>injection</topic><topic>Invertebrates</topic><topic>Ion Channel Gating</topic><topic>Life Sciences</topic><topic>Lytechinus</topic><topic>Lytechinus pictus</topic><topic>Lytechinus variegatus</topic><topic>Male</topic><topic>Marine</topic><topic>membrane currents</topic><topic>Membrane Potentials - drug effects</topic><topic>Microinjections</topic><topic>neomycin</topic><topic>Neomycin - pharmacology</topic><topic>Reproductive Biology</topic><topic>Sea Urchins - physiology</topic><topic>sperm</topic><topic>Sperm-Ovum Interactions - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Swann, Karl</creatorcontrib><creatorcontrib>McCulloh, David H.</creatorcontrib><creatorcontrib>McDougall, Alex</creatorcontrib><creatorcontrib>Chambers, Edward L.</creatorcontrib><creatorcontrib>Whitaker, Michael</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Swann, Karl</au><au>McCulloh, David H.</au><au>McDougall, Alex</au><au>Chambers, Edward L.</au><au>Whitaker, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm-induced currents at fertilization in sea urchin eggs injected with EGTA and neomycin</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1992-06-01</date><risdate>1992</risdate><volume>151</volume><issue>2</issue><spage>552</spage><epage>563</epage><pages>552-563</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><coden>DEBIAO</coden><abstract>Membrane currents were measured in single voltage-clamped sea urchin eggs (
Lytechinus pictus and
Lytechinus variegatus) that were injected with either EGTA or neomycin and inseminated. Although egg activation and the fertilization calcium wave were prevented by injection of either of these compounds, sperm attached and still elicited inward currents. Sperm-induced currents in EGTA-injected eggs had an abrupt onset, quickly reached a maximum, and then slowly declined in amplitude. Sperm incorporation occurred readily in EGTA-injected eggs. Similar results were obtained with another calcium chelator, BAPTA. In neomycin-injected eggs, sperm-induced currents generally had an abrupt onset and, in contrast to EGTA-injected eggs, the currents usually cut off rapidly. Sperm failed to enter the neomycin-injected eggs and the duration of sperm-induced currents in neomycin-injected eggs was markedly dependent upon the voltage-clamp holding potential, with shorter duration currents occurring at −70 than at −20 mV. The lability of the initial interaction between sperm and egg at negative holding potentials may explain why activation often fails when the egg membrane is voltage clamped at these potentials (Lynn
et al., Dev. Biol.
128, 305–323, 1988).</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>1318235</pmid><doi>10.1016/0012-1606(92)90193-K</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-0324-3836</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1606 |
| ispartof | Developmental biology, 1992-06, Vol.151 (2), p.552-563 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_03025613v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences biological fertilization Calcium Channels - drug effects Calcium Channels - physiology cell membranes Cellular Biology Echinodermata eggs EGTA Egtazic Acid - pharmacology Electrophysiology Female fertilization Fertilization - drug effects Fundamental and applied biological sciences. Psychology injection Invertebrates Ion Channel Gating Life Sciences Lytechinus Lytechinus pictus Lytechinus variegatus Male Marine membrane currents Membrane Potentials - drug effects Microinjections neomycin Neomycin - pharmacology Reproductive Biology Sea Urchins - physiology sperm Sperm-Ovum Interactions - drug effects |
| title | Sperm-induced currents at fertilization in sea urchin eggs injected with EGTA and neomycin |
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