Kinetic and structural characterization of therapeutic albumin chemical functionalization using complementary mass spectrometry techniques

•Chemical functionalization of HSA generating protein conjugates was investigated.•MALDI-TOF allowed estimating the number of species coupled to the peptide backbone.•MALDI-TOF provided the global reaction kinetic over the intact protein.•UPLC-MS/MS characterization enabled to precisely locate modif...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 2020-06, Vol.185, p.113242, Article 113242
Hauptverfasser:	Gahoual, Rabah, Bolbach, Gérard, Ould-Melha, Ikram, Clodic, Gilles, François, Yannis-Nicolas, Scherman, Daniel, Mignet, Nathalie, Houzé, Pascal
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical chemistry Bottom-up analysis Chemical functionalization Chemical Sciences Chemistry, Pharmaceutical - methods Crystallography, X-Ray Imidoesters - chemistry Immunoconjugates - analysis Immunoconjugates - chemistry Intact protein analysis Kinetic study Kinetics Lysine - analysis MALDI-TOF MS Protein conjugates Proteolysis Serum Albumin, Human - analysis Serum Albumin, Human - chemistry Serum Albumin, Human - ultrastructure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Tandem mass spectrometry Tandem Mass Spectrometry - methods
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