Crystallographic studies of the structured core domain of Knr4 fromSaccharomyces cerevisiae

Crystallographic studies of the structured core domain of Knr4 fromSaccharomyces cerevisiae

The potentially structured core domain of the intrinsically disordered protein Knr4 from Saccharomyces cerevisiae, comprising residues 80-340, was expressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method. Selenomethionine-containing (SeMet) protein was also purif...

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Veröffentlicht in:Acta crystallographica. Section F, Structural biology communications Structural biology communications, 2015, Vol.71 (9), p.1120-1124
Hauptverfasser: Julien, Sylviane, Tondl, Patrick, Durand, Fabien, Dagkessamanskaia, Adilia, van Tilbeurgh, Herman, François, Jean Marie, Mourey, Lionel, Zerbib, Didier, Martin-Yken, Hélène, Maveyraud, Laurent
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container_issue 9
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container_title Acta crystallographica. Section F, Structural biology communications
container_volume 71
creator Julien, Sylviane
Tondl, Patrick
Durand, Fabien
Dagkessamanskaia, Adilia
van Tilbeurgh, Herman
François, Jean Marie
Mourey, Lionel
Zerbib, Didier
Martin-Yken, Hélène
Maveyraud, Laurent
description The potentially structured core domain of the intrinsically disordered protein Knr4 from Saccharomyces cerevisiae, comprising residues 80-340, was expressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method. Selenomethionine-containing (SeMet) protein was also purified and crystallized. Crystals of both proteins belonged to space group P6 5 22, with unit-cell parameters a = b = 112.44, c = 265.21 Å for the native protein and a = b = 112.49, c = 262.21 Å for the SeMet protein, and diffracted to 3.50 and 3.60 Å resolution, respectively. There are two molecules in the asymmetric unit related by a twofold axis. The anomalous signal of selenium was recorded and yielded an electron-density map of sufficient quality to allow the identification of secondary-structure elements.
doi_str_mv 10.1107/s2053230x15012522
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title Crystallographic studies of the structured core domain of Knr4 fromSaccharomyces cerevisiae
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