Estimation of Kinetic Parameters Involved in Solid-Phase Immunoassays by Affinity Chromatography

Solid-phase immunoassays are based on the use of appropriate ligands and solid supports. In order to investigate the influence of the solid support on the interaction process, a predictive model of antibody/antigen interaction has been developed. Parameters of this model are estimated from dedicated...

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Veröffentlicht in:Industrial & engineering chemistry research 2020-06, Vol.59 (24), p.11113-11124
Hauptverfasser: Robin, Maëlenn, Tayakout-Fayolle, Mélaz, Pitault, Isabelle, Jallut, Christian, Drazek, Laurent
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container_end_page 11124
container_issue 24
container_start_page 11113
container_title Industrial & engineering chemistry research
container_volume 59
creator Robin, Maëlenn
Tayakout-Fayolle, Mélaz
Pitault, Isabelle
Jallut, Christian
Drazek, Laurent
description Solid-phase immunoassays are based on the use of appropriate ligands and solid supports. In order to investigate the influence of the solid support on the interaction process, a predictive model of antibody/antigen interaction has been developed. Parameters of this model are estimated from dedicated experiments like Surface Plas-mon Resonance and affinity chromatography by using least squares optimization techniques. Affinity chromatography is of great interest because, unlike Surface Plasmon Resonance, it enables estimation of kinetic parameters on any solid support used in immunoassays. The approach described above is illustrated through the kinetic modeling of the in-1
doi_str_mv 10.1021/acs.iecr.0c00922
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title Estimation of Kinetic Parameters Involved in Solid-Phase Immunoassays by Affinity Chromatography
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