SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation
Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a vers...
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| Veröffentlicht in: | Cell reports (Cambridge) 2020-07, Vol.32 (3), p.107934-107934, Article 107934 |
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| container_volume | 32 |
| creator | Ros, Oriol Baudet, Sarah Zagar, Yvrick Loulier, Karine Roche, Fiona Couvet, Sandrine Aghaie, Alain Atkins, Melody Louail, Alice Petit, Christine Metin, Christine Mechulam, Yves Nicol, Xavier |
| description | Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a versatile and genetically encoded chelator combining low- and high-affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.
[Display omitted]
•Development of SpiCee, a genetically encoded calcium scavenger•SpiCee inhibits calcium-dependent downstream pathways•SpiCee enables cell-specific manipulation of calcium-dependent processes in vivo•Subcellular targeting confers cell compartment specificity to SpiCee
Ros et al. develop SpiCee, a genetically encoded calcium chelator that enables the manipulation of this second messenger in single cells with subcellular specificity. SpiCee alters the migration of developing cortical neurons in vivo. Targeting of lipid rafts prevents axonal repulsion, whereas exclusion from this subcellular compartment does not. |
| doi_str_mv | 10.1016/j.celrep.2020.107934 |
| format | Article |
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[Display omitted]
•Development of SpiCee, a genetically encoded calcium scavenger•SpiCee inhibits calcium-dependent downstream pathways•SpiCee enables cell-specific manipulation of calcium-dependent processes in vivo•Subcellular targeting confers cell compartment specificity to SpiCee
Ros et al. develop SpiCee, a genetically encoded calcium chelator that enables the manipulation of this second messenger in single cells with subcellular specificity. SpiCee alters the migration of developing cortical neurons in vivo. Targeting of lipid rafts prevents axonal repulsion, whereas exclusion from this subcellular compartment does not.</description><identifier>ISSN: 2211-1247</identifier><identifier>EISSN: 2211-1247</identifier><identifier>DOI: 10.1016/j.celrep.2020.107934</identifier><identifier>PMID: 32697983</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Triphosphate - metabolism ; Animals ; axon guidance ; calcium ; Calcium - metabolism ; calcium buffer ; Calcium Signaling - drug effects ; calmodulin ; Cell Death - drug effects ; Cell Movement - drug effects ; Cell Survival - drug effects ; Cellular Biology ; Chelating Agents - pharmacology ; Development Biology ; EF hand ; Genetic Techniques ; HEK293 Cells ; Humans ; Life Sciences ; Mice, Inbred C57BL ; neuronal migration ; Neurons - cytology ; Neurons - drug effects ; parvalbumin ; Signal Transduction - drug effects ; single-cell pharmacology ; subcellular calcium manipulation ; subcellular compartment ; Subcellular Fractions - metabolism ; Thapsigargin - pharmacology</subject><ispartof>Cell reports (Cambridge), 2020-07, Vol.32 (3), p.107934-107934, Article 107934</ispartof><rights>2020 The Authors</rights><rights>Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-361c65411043e5652983fa50107ee2c176d7f14d40b8cb7be0a064e263466f1f3</citedby><cites>FETCH-LOGICAL-c508t-361c65411043e5652983fa50107ee2c176d7f14d40b8cb7be0a064e263466f1f3</cites><orcidid>0000-0003-3676-1891 ; 0000-0002-0310-8047 ; 0000-0001-6216-2708 ; 0000-0002-9069-002X ; 0000-0002-3247-7087</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,860,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32697983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02992312$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Ros, Oriol</creatorcontrib><creatorcontrib>Baudet, Sarah</creatorcontrib><creatorcontrib>Zagar, Yvrick</creatorcontrib><creatorcontrib>Loulier, Karine</creatorcontrib><creatorcontrib>Roche, Fiona</creatorcontrib><creatorcontrib>Couvet, Sandrine</creatorcontrib><creatorcontrib>Aghaie, Alain</creatorcontrib><creatorcontrib>Atkins, Melody</creatorcontrib><creatorcontrib>Louail, Alice</creatorcontrib><creatorcontrib>Petit, Christine</creatorcontrib><creatorcontrib>Metin, Christine</creatorcontrib><creatorcontrib>Mechulam, Yves</creatorcontrib><creatorcontrib>Nicol, Xavier</creatorcontrib><title>SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation</title><title>Cell reports (Cambridge)</title><addtitle>Cell Rep</addtitle><description>Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a versatile and genetically encoded chelator combining low- and high-affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.
[Display omitted]
•Development of SpiCee, a genetically encoded calcium scavenger•SpiCee inhibits calcium-dependent downstream pathways•SpiCee enables cell-specific manipulation of calcium-dependent processes in vivo•Subcellular targeting confers cell compartment specificity to SpiCee
Ros et al. develop SpiCee, a genetically encoded calcium chelator that enables the manipulation of this second messenger in single cells with subcellular specificity. SpiCee alters the migration of developing cortical neurons in vivo. Targeting of lipid rafts prevents axonal repulsion, whereas exclusion from this subcellular compartment does not.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>axon guidance</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>calcium buffer</subject><subject>Calcium Signaling - drug effects</subject><subject>calmodulin</subject><subject>Cell Death - drug effects</subject><subject>Cell Movement - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cellular Biology</subject><subject>Chelating Agents - pharmacology</subject><subject>Development Biology</subject><subject>EF hand</subject><subject>Genetic Techniques</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Mice, Inbred C57BL</subject><subject>neuronal migration</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>parvalbumin</subject><subject>Signal Transduction - drug effects</subject><subject>single-cell pharmacology</subject><subject>subcellular calcium manipulation</subject><subject>subcellular compartment</subject><subject>Subcellular Fractions - metabolism</subject><subject>Thapsigargin - pharmacology</subject><issn>2211-1247</issn><issn>2211-1247</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFFr2zAQgMVYWUPafzCGH9cHZzpJluM9FIJp00HCHpI9C1k-MwXH8iS70H9fGbehT9OLdMd3d7qPkK9AV0BB_jitDLYe-xWjbErlBRefyIIxgBSYyD9_eF-T2xBONB5JAQrxhVxzJou8WPMF2R96WyL-TDbJFjscrEmOzrVJ43xyGKs4pR1b7RPd1UkZg_TQo7FNxErdGjuek73ubB-Zwbruhlw1ug14-3YvyZ_Hh2P5lO5-b3-Vm11qMroeUi7ByEwAUMExkxmLX2l0RuMeiMxALuu8AVELWq1NlVdINZUCmeRCygYaviR3c9-_ulW9t2ftX5TTVj1tdmrKUVYUjAN7hsh-n9neu38jhkGdbZj20h26MSgmmMy4XBc0omJGjXcheGwuvYGqybs6qdm7mryr2Xss-_Y2YazOWF-K3i1H4H4GMDp5tuhVMBY7g7X1aAZVO_v_Ca9fdZHF</recordid><startdate>20200721</startdate><enddate>20200721</enddate><creator>Ros, Oriol</creator><creator>Baudet, Sarah</creator><creator>Zagar, Yvrick</creator><creator>Loulier, Karine</creator><creator>Roche, Fiona</creator><creator>Couvet, Sandrine</creator><creator>Aghaie, Alain</creator><creator>Atkins, Melody</creator><creator>Louail, Alice</creator><creator>Petit, Christine</creator><creator>Metin, Christine</creator><creator>Mechulam, Yves</creator><creator>Nicol, Xavier</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0003-3676-1891</orcidid><orcidid>https://orcid.org/0000-0002-0310-8047</orcidid><orcidid>https://orcid.org/0000-0001-6216-2708</orcidid><orcidid>https://orcid.org/0000-0002-9069-002X</orcidid><orcidid>https://orcid.org/0000-0002-3247-7087</orcidid></search><sort><creationdate>20200721</creationdate><title>SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation</title><author>Ros, Oriol ; Baudet, Sarah ; Zagar, Yvrick ; Loulier, Karine ; Roche, Fiona ; Couvet, Sandrine ; Aghaie, Alain ; Atkins, Melody ; Louail, Alice ; Petit, Christine ; Metin, Christine ; Mechulam, Yves ; Nicol, Xavier</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-361c65411043e5652983fa50107ee2c176d7f14d40b8cb7be0a064e263466f1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>axon guidance</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>calcium buffer</topic><topic>Calcium Signaling - drug effects</topic><topic>calmodulin</topic><topic>Cell Death - drug effects</topic><topic>Cell Movement - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cellular Biology</topic><topic>Chelating Agents - pharmacology</topic><topic>Development Biology</topic><topic>EF hand</topic><topic>Genetic Techniques</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Mice, Inbred C57BL</topic><topic>neuronal migration</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>parvalbumin</topic><topic>Signal Transduction - drug effects</topic><topic>single-cell pharmacology</topic><topic>subcellular calcium manipulation</topic><topic>subcellular compartment</topic><topic>Subcellular Fractions - metabolism</topic><topic>Thapsigargin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ros, Oriol</creatorcontrib><creatorcontrib>Baudet, Sarah</creatorcontrib><creatorcontrib>Zagar, Yvrick</creatorcontrib><creatorcontrib>Loulier, Karine</creatorcontrib><creatorcontrib>Roche, Fiona</creatorcontrib><creatorcontrib>Couvet, Sandrine</creatorcontrib><creatorcontrib>Aghaie, Alain</creatorcontrib><creatorcontrib>Atkins, Melody</creatorcontrib><creatorcontrib>Louail, Alice</creatorcontrib><creatorcontrib>Petit, Christine</creatorcontrib><creatorcontrib>Metin, Christine</creatorcontrib><creatorcontrib>Mechulam, Yves</creatorcontrib><creatorcontrib>Nicol, Xavier</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Cell reports (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ros, Oriol</au><au>Baudet, Sarah</au><au>Zagar, Yvrick</au><au>Loulier, Karine</au><au>Roche, Fiona</au><au>Couvet, Sandrine</au><au>Aghaie, Alain</au><au>Atkins, Melody</au><au>Louail, Alice</au><au>Petit, Christine</au><au>Metin, Christine</au><au>Mechulam, Yves</au><au>Nicol, Xavier</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation</atitle><jtitle>Cell reports (Cambridge)</jtitle><addtitle>Cell Rep</addtitle><date>2020-07-21</date><risdate>2020</risdate><volume>32</volume><issue>3</issue><spage>107934</spage><epage>107934</epage><pages>107934-107934</pages><artnum>107934</artnum><issn>2211-1247</issn><eissn>2211-1247</eissn><abstract>Calcium is a second messenger crucial to a myriad of cellular processes ranging from regulation of metabolism and cell survival to vesicle release and motility. Current strategies to directly manipulate endogenous calcium signals lack cellular and subcellular specificity. We introduce SpiCee, a versatile and genetically encoded chelator combining low- and high-affinity sites for calcium. This scavenger enables altering endogenous calcium signaling and functions in single cells in vitro and in vivo with biochemically controlled subcellular resolution. SpiCee paves the way to investigate local calcium signaling in vivo and directly manipulate this second messenger for therapeutic use.
[Display omitted]
•Development of SpiCee, a genetically encoded calcium scavenger•SpiCee inhibits calcium-dependent downstream pathways•SpiCee enables cell-specific manipulation of calcium-dependent processes in vivo•Subcellular targeting confers cell compartment specificity to SpiCee
Ros et al. develop SpiCee, a genetically encoded calcium chelator that enables the manipulation of this second messenger in single cells with subcellular specificity. SpiCee alters the migration of developing cortical neurons in vivo. Targeting of lipid rafts prevents axonal repulsion, whereas exclusion from this subcellular compartment does not.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32697983</pmid><doi>10.1016/j.celrep.2020.107934</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-3676-1891</orcidid><orcidid>https://orcid.org/0000-0002-0310-8047</orcidid><orcidid>https://orcid.org/0000-0001-6216-2708</orcidid><orcidid>https://orcid.org/0000-0002-9069-002X</orcidid><orcidid>https://orcid.org/0000-0002-3247-7087</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenosine Triphosphate - metabolism Animals axon guidance calcium Calcium - metabolism calcium buffer Calcium Signaling - drug effects calmodulin Cell Death - drug effects Cell Movement - drug effects Cell Survival - drug effects Cellular Biology Chelating Agents - pharmacology Development Biology EF hand Genetic Techniques HEK293 Cells Humans Life Sciences Mice, Inbred C57BL neuronal migration Neurons - cytology Neurons - drug effects parvalbumin Signal Transduction - drug effects single-cell pharmacology subcellular calcium manipulation subcellular compartment Subcellular Fractions - metabolism Thapsigargin - pharmacology |
| title | SpiCee: A Genetic Tool for Subcellular and Cell-Specific Calcium Manipulation |
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