New Functions of the Thylakoid Membrane Proteome of Arabidopsis thaliana Revealed by a Simple, Fast, and Versatile Fractionation Strategy

Identification of membrane proteomes remains challenging. Here, we present a simple, fast, and scalable off-line procedure based on three-phase partitioning with butanol to fractionate membrane proteomes in combination with both in-gel and in-solution digestions and mass spectrometry. This should he...

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Veröffentlicht in:The Journal of biological chemistry 2004-11, Vol.279 (47), p.49367-49383
Hauptverfasser: Peltier, Jean-Benoit, Ytterberg, A Jimmy, Sun, Qi, van Wijk, Klaas J
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Sprache:eng
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