Development of a film-assisted honeybee egg collection system (FECS)
Despite the huge potential of genome editing in honeybee research and breeding programs, only a few successful studies have been reported, implying the presence of several obstacles. One such obstacle is the difficulty in obtaining a large quantity of young eggs. To facilitate the overall procedures...
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| Veröffentlicht in: | Apidologie 2019-12, Vol.50 (6), p.804-810 |
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| container_title | Apidologie |
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| creator | Lee, Jaeho Lee, Si Hyeock |
| description | Despite the huge potential of genome editing in honeybee research and breeding programs, only a few successful studies have been reported, implying the presence of several obstacles. One such obstacle is the difficulty in obtaining a large quantity of young eggs. To facilitate the overall procedures for egg collection and microinjection, we developed the film-assisted honeybee egg collection system (FECS), in which a removable transparent film is used as the platform for consecutive collection and injection of embryos. The FECS provides a significantly higher (~ 2-fold) collection efficiency and usability compared with the conventional plug-based queen rearing system while maintaining a high hatching rate. The system can be readily utilized for various studies that require a large number of honeybee eggs, including honeybee genome editing. |
| doi_str_mv | 10.1007/s13592-019-00687-8 |
| format | Article |
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| ispartof | Apidologie, 2019-12, Vol.50 (6), p.804-810 |
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| language | eng |
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| source | SpringerLink Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Bees Biomedical and Life Sciences Breeding Collection Editing Eggs Embryos Entomology Genomes Hatching Life Sciences Microinjection Original Article |
| title | Development of a film-assisted honeybee egg collection system (FECS) |
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