Screening of Gastrointestinal Lipase Inhibitors Produced by Microorganisms Isolated from Soil and Lake Sediments
Gastrointestinal lipase inhibitors are molecules of pharmaceutical interest due to their use as anti-obesity drugs. In this study, forty strains isolated from soil and sediments were identified with the ability to produce inhibition of gastrointestinal lipase activity. The biomass extract of these s...
Gespeichert in:
| Veröffentlicht in: | International microbiology 2020-05, Vol.23 (2), p.335-343 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 343 |
|---|---|
| container_issue | 2 |
| container_start_page | 335 |
| container_title | International microbiology |
| container_volume | 23 |
| creator | Camacho-Ruiz, Maria Angeles Ordaz, Enrique Kirchmayr, Manuel R. Esquivel-Solís, Hugo Asaff-Torres, Ali Mateos-Díaz, Juan Carlos Carriѐre, Frédéric Rodríguez, Jorge A. |
| description | Gastrointestinal lipase inhibitors are molecules of pharmaceutical interest due to their use as anti-obesity drugs. In this study, forty strains isolated from soil and sediments were identified with the ability to produce inhibition of gastrointestinal lipase activity. The biomass extract of these strains showed at least 50% inhibition in the hydrolysis of tributyrin by recombinant human pancreatic lipase (rHPL) or rabbit gastric lipase (RGL) by in vitro assays. Based on gene sequencing, the isolates were identified mainly as Streptomycetes. Moreover, none of the identified strains has been reported to be lipase inhibitor producers, so they can be viewed as potential sources for obtaining new drugs. IC
50
values of the three best inhibitor extracts showed that AC104-10 was the most promising strain for production of gastrointestinal lipase inhibitors. AC104-10 shows 99% homology (16S rRNA gene fragment) to
Streptomyces cinereoruber
strain NBRC 12756. An inhibitory study over trypsin activity revealed that AC104-10 extract, as well as THL, had no significant effect on the activity of this protease, showing its specificity for lipases. In addition, analyzes by MALDI-TOF mass spectrometry of the enzyme-inhibitor complex revealed that there is a covalent interaction of the AC104-10 inhibitor with the catalytic serine of the pancreatic lipase, and that the molecular weight of the inhibitor is approximately 686.19 Da. |
| doi_str_mv | 10.1007/s10123-019-00107-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02904324v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2324911498</sourcerecordid><originalsourceid>FETCH-LOGICAL-c453t-c235a54097a21ca713a7921c0afc3c973910623e7251b82e6252f2975d66be643</originalsourceid><addsrcrecordid>eNp9kU9v1DAQxSMEon_gC3BAlri0h8CMHcfxsapou1IqkBbOluM4W5fEXuwEab89LilF4tCTR57fPI_fK4p3CB8RQHxKCEhZCShLAARRHl4Ux1hjU6IE_jLXyGRZC5BHxUlK9xkSdQOviyOGDWUU2HGx35porXd-R8JArnWaY3B-tml2Xo-kdXudLNn4O9e5OcREvsbQL8b2pDuQW2diCHGnvUtTIpsURj3n1hDDRLbBjUT7nrT6hyVb27vJ-jm9KV4Nekz27eN5Wny_-vzt8qZsv1xvLi_a0lSczaWhjGtegRSaotECmRYyV6AHw4wUTCLUlFlBOXYNtTXldKBS8L6uO1tX7LQ4X3Xv9Kj20U06HlTQTt1ctOrhDqiEitHqF2b2bGX3Mfxc8t_V5JKx46i9DUtS2atKIlayyeiH_9D7sMRsVaZ4w6uG5b0yRVcq-5NStMPTBgjqITu1ZqdydupPduqQh94_Si_dZPunkb9hZYCtQMotv7Px39vPyP4GJwSjOQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2585483106</pqid></control><display><type>article</type><title>Screening of Gastrointestinal Lipase Inhibitors Produced by Microorganisms Isolated from Soil and Lake Sediments</title><source>SpringerLink Journals - AutoHoldings</source><creator>Camacho-Ruiz, Maria Angeles ; Ordaz, Enrique ; Kirchmayr, Manuel R. ; Esquivel-Solís, Hugo ; Asaff-Torres, Ali ; Mateos-Díaz, Juan Carlos ; Carriѐre, Frédéric ; Rodríguez, Jorge A.</creator><creatorcontrib>Camacho-Ruiz, Maria Angeles ; Ordaz, Enrique ; Kirchmayr, Manuel R. ; Esquivel-Solís, Hugo ; Asaff-Torres, Ali ; Mateos-Díaz, Juan Carlos ; Carriѐre, Frédéric ; Rodríguez, Jorge A.</creatorcontrib><description>Gastrointestinal lipase inhibitors are molecules of pharmaceutical interest due to their use as anti-obesity drugs. In this study, forty strains isolated from soil and sediments were identified with the ability to produce inhibition of gastrointestinal lipase activity. The biomass extract of these strains showed at least 50% inhibition in the hydrolysis of tributyrin by recombinant human pancreatic lipase (rHPL) or rabbit gastric lipase (RGL) by in vitro assays. Based on gene sequencing, the isolates were identified mainly as Streptomycetes. Moreover, none of the identified strains has been reported to be lipase inhibitor producers, so they can be viewed as potential sources for obtaining new drugs. IC
50
values of the three best inhibitor extracts showed that AC104-10 was the most promising strain for production of gastrointestinal lipase inhibitors. AC104-10 shows 99% homology (16S rRNA gene fragment) to
Streptomyces cinereoruber
strain NBRC 12756. An inhibitory study over trypsin activity revealed that AC104-10 extract, as well as THL, had no significant effect on the activity of this protease, showing its specificity for lipases. In addition, analyzes by MALDI-TOF mass spectrometry of the enzyme-inhibitor complex revealed that there is a covalent interaction of the AC104-10 inhibitor with the catalytic serine of the pancreatic lipase, and that the molecular weight of the inhibitor is approximately 686.19 Da.</description><identifier>ISSN: 1139-6709</identifier><identifier>EISSN: 1618-1905</identifier><identifier>DOI: 10.1007/s10123-019-00107-y</identifier><identifier>PMID: 31823203</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Applied Microbiology ; Biochemistry, Molecular Biology ; Biomedical and Life Sciences ; Drugs ; Eukaryotic Microbiology ; Gene sequencing ; Homology ; Inhibitors ; Lake sediments ; Life Sciences ; Lipase ; Mass spectrometry ; Mass spectroscopy ; Medical Microbiology ; Microbial Ecology ; Microbiology ; Microbiology and Parasitology ; Microorganisms ; Molecular weight ; Original Article ; Pancreas ; rRNA 16S ; Sediments ; Soil microorganisms ; Soils ; Strains (organisms) ; Streptomycetes ; Trypsin ; Water analysis</subject><ispartof>International microbiology, 2020-05, Vol.23 (2), p.335-343</ispartof><rights>Springer Nature Switzerland AG 2019</rights><rights>Copyright Spanish Society for Microbiology May 2020</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-c235a54097a21ca713a7921c0afc3c973910623e7251b82e6252f2975d66be643</citedby><cites>FETCH-LOGICAL-c453t-c235a54097a21ca713a7921c0afc3c973910623e7251b82e6252f2975d66be643</cites><orcidid>0000-0002-6723-6654 ; 0000-0002-3685-3094 ; 0000-0002-9857-9115 ; 0000-0003-4848-9418 ; 0000-0002-7585-230X ; 0000-0001-5619-894X ; 0000-0001-9544-2917</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10123-019-00107-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10123-019-00107-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31823203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02904324$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Camacho-Ruiz, Maria Angeles</creatorcontrib><creatorcontrib>Ordaz, Enrique</creatorcontrib><creatorcontrib>Kirchmayr, Manuel R.</creatorcontrib><creatorcontrib>Esquivel-Solís, Hugo</creatorcontrib><creatorcontrib>Asaff-Torres, Ali</creatorcontrib><creatorcontrib>Mateos-Díaz, Juan Carlos</creatorcontrib><creatorcontrib>Carriѐre, Frédéric</creatorcontrib><creatorcontrib>Rodríguez, Jorge A.</creatorcontrib><title>Screening of Gastrointestinal Lipase Inhibitors Produced by Microorganisms Isolated from Soil and Lake Sediments</title><title>International microbiology</title><addtitle>Int Microbiol</addtitle><addtitle>Int Microbiol</addtitle><description>Gastrointestinal lipase inhibitors are molecules of pharmaceutical interest due to their use as anti-obesity drugs. In this study, forty strains isolated from soil and sediments were identified with the ability to produce inhibition of gastrointestinal lipase activity. The biomass extract of these strains showed at least 50% inhibition in the hydrolysis of tributyrin by recombinant human pancreatic lipase (rHPL) or rabbit gastric lipase (RGL) by in vitro assays. Based on gene sequencing, the isolates were identified mainly as Streptomycetes. Moreover, none of the identified strains has been reported to be lipase inhibitor producers, so they can be viewed as potential sources for obtaining new drugs. IC
50
values of the three best inhibitor extracts showed that AC104-10 was the most promising strain for production of gastrointestinal lipase inhibitors. AC104-10 shows 99% homology (16S rRNA gene fragment) to
Streptomyces cinereoruber
strain NBRC 12756. An inhibitory study over trypsin activity revealed that AC104-10 extract, as well as THL, had no significant effect on the activity of this protease, showing its specificity for lipases. In addition, analyzes by MALDI-TOF mass spectrometry of the enzyme-inhibitor complex revealed that there is a covalent interaction of the AC104-10 inhibitor with the catalytic serine of the pancreatic lipase, and that the molecular weight of the inhibitor is approximately 686.19 Da.</description><subject>Applied Microbiology</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biomedical and Life Sciences</subject><subject>Drugs</subject><subject>Eukaryotic Microbiology</subject><subject>Gene sequencing</subject><subject>Homology</subject><subject>Inhibitors</subject><subject>Lake sediments</subject><subject>Life Sciences</subject><subject>Lipase</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Microorganisms</subject><subject>Molecular weight</subject><subject>Original Article</subject><subject>Pancreas</subject><subject>rRNA 16S</subject><subject>Sediments</subject><subject>Soil microorganisms</subject><subject>Soils</subject><subject>Strains (organisms)</subject><subject>Streptomycetes</subject><subject>Trypsin</subject><subject>Water analysis</subject><issn>1139-6709</issn><issn>1618-1905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kU9v1DAQxSMEon_gC3BAlri0h8CMHcfxsapou1IqkBbOluM4W5fEXuwEab89LilF4tCTR57fPI_fK4p3CB8RQHxKCEhZCShLAARRHl4Ux1hjU6IE_jLXyGRZC5BHxUlK9xkSdQOviyOGDWUU2HGx35porXd-R8JArnWaY3B-tml2Xo-kdXudLNn4O9e5OcREvsbQL8b2pDuQW2diCHGnvUtTIpsURj3n1hDDRLbBjUT7nrT6hyVb27vJ-jm9KV4Nekz27eN5Wny_-vzt8qZsv1xvLi_a0lSczaWhjGtegRSaotECmRYyV6AHw4wUTCLUlFlBOXYNtTXldKBS8L6uO1tX7LQ4X3Xv9Kj20U06HlTQTt1ctOrhDqiEitHqF2b2bGX3Mfxc8t_V5JKx46i9DUtS2atKIlayyeiH_9D7sMRsVaZ4w6uG5b0yRVcq-5NStMPTBgjqITu1ZqdydupPduqQh94_Si_dZPunkb9hZYCtQMotv7Px39vPyP4GJwSjOQ</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Camacho-Ruiz, Maria Angeles</creator><creator>Ordaz, Enrique</creator><creator>Kirchmayr, Manuel R.</creator><creator>Esquivel-Solís, Hugo</creator><creator>Asaff-Torres, Ali</creator><creator>Mateos-Díaz, Juan Carlos</creator><creator>Carriѐre, Frédéric</creator><creator>Rodríguez, Jorge A.</creator><general>Springer International Publishing</general><general>Spanish Society for Microbiology</general><general>Springer Nature (Hemeroteca Científica Catalana)</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><orcidid>https://orcid.org/0000-0002-6723-6654</orcidid><orcidid>https://orcid.org/0000-0002-3685-3094</orcidid><orcidid>https://orcid.org/0000-0002-9857-9115</orcidid><orcidid>https://orcid.org/0000-0003-4848-9418</orcidid><orcidid>https://orcid.org/0000-0002-7585-230X</orcidid><orcidid>https://orcid.org/0000-0001-5619-894X</orcidid><orcidid>https://orcid.org/0000-0001-9544-2917</orcidid></search><sort><creationdate>20200501</creationdate><title>Screening of Gastrointestinal Lipase Inhibitors Produced by Microorganisms Isolated from Soil and Lake Sediments</title><author>Camacho-Ruiz, Maria Angeles ; Ordaz, Enrique ; Kirchmayr, Manuel R. ; Esquivel-Solís, Hugo ; Asaff-Torres, Ali ; Mateos-Díaz, Juan Carlos ; Carriѐre, Frédéric ; Rodríguez, Jorge A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-c235a54097a21ca713a7921c0afc3c973910623e7251b82e6252f2975d66be643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Applied Microbiology</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biomedical and Life Sciences</topic><topic>Drugs</topic><topic>Eukaryotic Microbiology</topic><topic>Gene sequencing</topic><topic>Homology</topic><topic>Inhibitors</topic><topic>Lake sediments</topic><topic>Life Sciences</topic><topic>Lipase</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Microorganisms</topic><topic>Molecular weight</topic><topic>Original Article</topic><topic>Pancreas</topic><topic>rRNA 16S</topic><topic>Sediments</topic><topic>Soil microorganisms</topic><topic>Soils</topic><topic>Strains (organisms)</topic><topic>Streptomycetes</topic><topic>Trypsin</topic><topic>Water analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Camacho-Ruiz, Maria Angeles</creatorcontrib><creatorcontrib>Ordaz, Enrique</creatorcontrib><creatorcontrib>Kirchmayr, Manuel R.</creatorcontrib><creatorcontrib>Esquivel-Solís, Hugo</creatorcontrib><creatorcontrib>Asaff-Torres, Ali</creatorcontrib><creatorcontrib>Mateos-Díaz, Juan Carlos</creatorcontrib><creatorcontrib>Carriѐre, Frédéric</creatorcontrib><creatorcontrib>Rodríguez, Jorge A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>International microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Camacho-Ruiz, Maria Angeles</au><au>Ordaz, Enrique</au><au>Kirchmayr, Manuel R.</au><au>Esquivel-Solís, Hugo</au><au>Asaff-Torres, Ali</au><au>Mateos-Díaz, Juan Carlos</au><au>Carriѐre, Frédéric</au><au>Rodríguez, Jorge A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of Gastrointestinal Lipase Inhibitors Produced by Microorganisms Isolated from Soil and Lake Sediments</atitle><jtitle>International microbiology</jtitle><stitle>Int Microbiol</stitle><addtitle>Int Microbiol</addtitle><date>2020-05-01</date><risdate>2020</risdate><volume>23</volume><issue>2</issue><spage>335</spage><epage>343</epage><pages>335-343</pages><issn>1139-6709</issn><eissn>1618-1905</eissn><abstract>Gastrointestinal lipase inhibitors are molecules of pharmaceutical interest due to their use as anti-obesity drugs. In this study, forty strains isolated from soil and sediments were identified with the ability to produce inhibition of gastrointestinal lipase activity. The biomass extract of these strains showed at least 50% inhibition in the hydrolysis of tributyrin by recombinant human pancreatic lipase (rHPL) or rabbit gastric lipase (RGL) by in vitro assays. Based on gene sequencing, the isolates were identified mainly as Streptomycetes. Moreover, none of the identified strains has been reported to be lipase inhibitor producers, so they can be viewed as potential sources for obtaining new drugs. IC
50
values of the three best inhibitor extracts showed that AC104-10 was the most promising strain for production of gastrointestinal lipase inhibitors. AC104-10 shows 99% homology (16S rRNA gene fragment) to
Streptomyces cinereoruber
strain NBRC 12756. An inhibitory study over trypsin activity revealed that AC104-10 extract, as well as THL, had no significant effect on the activity of this protease, showing its specificity for lipases. In addition, analyzes by MALDI-TOF mass spectrometry of the enzyme-inhibitor complex revealed that there is a covalent interaction of the AC104-10 inhibitor with the catalytic serine of the pancreatic lipase, and that the molecular weight of the inhibitor is approximately 686.19 Da.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>31823203</pmid><doi>10.1007/s10123-019-00107-y</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-6723-6654</orcidid><orcidid>https://orcid.org/0000-0002-3685-3094</orcidid><orcidid>https://orcid.org/0000-0002-9857-9115</orcidid><orcidid>https://orcid.org/0000-0003-4848-9418</orcidid><orcidid>https://orcid.org/0000-0002-7585-230X</orcidid><orcidid>https://orcid.org/0000-0001-5619-894X</orcidid><orcidid>https://orcid.org/0000-0001-9544-2917</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1139-6709 |
| ispartof | International microbiology, 2020-05, Vol.23 (2), p.335-343 |
| issn | 1139-6709 1618-1905 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02904324v1 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Applied Microbiology Biochemistry, Molecular Biology Biomedical and Life Sciences Drugs Eukaryotic Microbiology Gene sequencing Homology Inhibitors Lake sediments Life Sciences Lipase Mass spectrometry Mass spectroscopy Medical Microbiology Microbial Ecology Microbiology Microbiology and Parasitology Microorganisms Molecular weight Original Article Pancreas rRNA 16S Sediments Soil microorganisms Soils Strains (organisms) Streptomycetes Trypsin Water analysis |
| title | Screening of Gastrointestinal Lipase Inhibitors Produced by Microorganisms Isolated from Soil and Lake Sediments |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T02%3A41%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Screening%20of%20Gastrointestinal%20Lipase%20Inhibitors%20Produced%20by%20Microorganisms%20Isolated%20from%20Soil%20and%20Lake%20Sediments&rft.jtitle=International%20microbiology&rft.au=Camacho-Ruiz,%20Maria%20Angeles&rft.date=2020-05-01&rft.volume=23&rft.issue=2&rft.spage=335&rft.epage=343&rft.pages=335-343&rft.issn=1139-6709&rft.eissn=1618-1905&rft_id=info:doi/10.1007/s10123-019-00107-y&rft_dat=%3Cproquest_hal_p%3E2324911498%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2585483106&rft_id=info:pmid/31823203&rfr_iscdi=true |