Vasoactive intestinal peptide-induced neurite remodeling in human neuroblastoma SH-SY5Y cells implicates the Cdc42 GTPase and is independent of Ras-ERK pathway
Vasoactive intestinal peptide (VIP) is known to regulate proliferation or differentiation in normal and tumoral cells. SH-SY5Y is a differentiated cell subclone derived from the SK-N-SH human neuroblastoma cell line and possess all the components for an autocrine action of VIP. In the present study,...
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| Veröffentlicht in: | Experimental cell research 2004-10, Vol.299 (2), p.511-524 |
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| creator | Alleaume, Céline Eychène, Alain Harnois, Thomas Bourmeyster, Nicolas Constantin, Bruno Caigneaux, Evelyne Muller, Jean-Marc Philippe, Michel |
| description | Vasoactive intestinal peptide (VIP) is known to regulate proliferation or differentiation in normal and tumoral cells. SH-SY5Y is a differentiated cell subclone derived from the SK-N-SH human neuroblastoma cell line and possess all the components for an autocrine action of VIP. In the present study, we investigated the morphological changes and intracellular signaling pathways occurring upon VIP treatment of SH-SY5Y cells. VIP induced an early remodeling of cell projections: a branched neurite network spread out and prominent varicosities developed along neurites. Although activated by VIP, the Ras/ERK pathway was not required for the remodeling process. In contrast, pull-down experiments revealed a strong Cdc42 activation by VIP while expression of a dominant-negative Cdc42 prevented the VIP-induced neurite changes, suggesting an important role for this small GTPase in the process. These data provide the first evidence for a regulation of the activity of Rho family GTPases by VIP and bring new insights in the signaling pathways implicated in neurite remodeling process induced by VIP in neuroblastoma cells. |
| doi_str_mv | 10.1016/j.yexcr.2004.06.016 |
| format | Article |
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SH-SY5Y is a differentiated cell subclone derived from the SK-N-SH human neuroblastoma cell line and possess all the components for an autocrine action of VIP. In the present study, we investigated the morphological changes and intracellular signaling pathways occurring upon VIP treatment of SH-SY5Y cells. VIP induced an early remodeling of cell projections: a branched neurite network spread out and prominent varicosities developed along neurites. Although activated by VIP, the Ras/ERK pathway was not required for the remodeling process. In contrast, pull-down experiments revealed a strong Cdc42 activation by VIP while expression of a dominant-negative Cdc42 prevented the VIP-induced neurite changes, suggesting an important role for this small GTPase in the process. These data provide the first evidence for a regulation of the activity of Rho family GTPases by VIP and bring new insights in the signaling pathways implicated in neurite remodeling process induced by VIP in neuroblastoma cells.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/j.yexcr.2004.06.016</identifier><identifier>PMID: 15350548</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cancer ; Cdc42 ; cdc42 GTP-Binding Protein - metabolism ; Cellular Biology ; ERK ; Genes, Dominant ; Humans ; Life Sciences ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 ; Mitogen-Activated Protein Kinases - metabolism ; Neurite remodeling ; Neurites - drug effects ; Neurites - metabolism ; Neuroblastoma - metabolism ; Neuroblastoma - pathology ; Neuroprotective Agents - pharmacology ; rac1 GTP-Binding Protein - metabolism ; Ras ; ras Proteins - metabolism ; SH-SY5Y neuroblastoma cells ; Signal Transduction ; Subcellular Processes ; Tumor Cells, Cultured ; Vasoactive Intestinal Peptide - pharmacology ; VIP</subject><ispartof>Experimental cell research, 2004-10, Vol.299 (2), p.511-524</ispartof><rights>2004 Elsevier Inc.</rights><rights>Copyright 2004 Elsevier Inc.</rights><rights>Attribution - NonCommercial - NoDerivatives</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-4596b5c4fb08d699ab9c80299ca37d4a76fb1bc3802d2b9d7c69c55f11a32d4d3</citedby><cites>FETCH-LOGICAL-c420t-4596b5c4fb08d699ab9c80299ca37d4a76fb1bc3802d2b9d7c69c55f11a32d4d3</cites><orcidid>0000-0003-1668-581X ; 0000-0002-4582-8990 ; 0000-0002-9563-5142 ; 0000-0001-6009-7471 ; 0000-0002-6818-7225 ; 0000-0003-3359-6341</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yexcr.2004.06.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15350548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02880277$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Alleaume, Céline</creatorcontrib><creatorcontrib>Eychène, Alain</creatorcontrib><creatorcontrib>Harnois, Thomas</creatorcontrib><creatorcontrib>Bourmeyster, Nicolas</creatorcontrib><creatorcontrib>Constantin, Bruno</creatorcontrib><creatorcontrib>Caigneaux, Evelyne</creatorcontrib><creatorcontrib>Muller, Jean-Marc</creatorcontrib><creatorcontrib>Philippe, Michel</creatorcontrib><title>Vasoactive intestinal peptide-induced neurite remodeling in human neuroblastoma SH-SY5Y cells implicates the Cdc42 GTPase and is independent of Ras-ERK pathway</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Vasoactive intestinal peptide (VIP) is known to regulate proliferation or differentiation in normal and tumoral cells. SH-SY5Y is a differentiated cell subclone derived from the SK-N-SH human neuroblastoma cell line and possess all the components for an autocrine action of VIP. In the present study, we investigated the morphological changes and intracellular signaling pathways occurring upon VIP treatment of SH-SY5Y cells. VIP induced an early remodeling of cell projections: a branched neurite network spread out and prominent varicosities developed along neurites. Although activated by VIP, the Ras/ERK pathway was not required for the remodeling process. In contrast, pull-down experiments revealed a strong Cdc42 activation by VIP while expression of a dominant-negative Cdc42 prevented the VIP-induced neurite changes, suggesting an important role for this small GTPase in the process. 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Eychène, Alain ; Harnois, Thomas ; Bourmeyster, Nicolas ; Constantin, Bruno ; Caigneaux, Evelyne ; Muller, Jean-Marc ; Philippe, Michel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-4596b5c4fb08d699ab9c80299ca37d4a76fb1bc3802d2b9d7c69c55f11a32d4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Cancer</topic><topic>Cdc42</topic><topic>cdc42 GTP-Binding Protein - metabolism</topic><topic>Cellular Biology</topic><topic>ERK</topic><topic>Genes, Dominant</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Neurite remodeling</topic><topic>Neurites - drug effects</topic><topic>Neurites - metabolism</topic><topic>Neuroblastoma - metabolism</topic><topic>Neuroblastoma - pathology</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>rac1 GTP-Binding Protein - metabolism</topic><topic>Ras</topic><topic>ras Proteins - metabolism</topic><topic>SH-SY5Y neuroblastoma cells</topic><topic>Signal Transduction</topic><topic>Subcellular Processes</topic><topic>Tumor Cells, Cultured</topic><topic>Vasoactive Intestinal Peptide - pharmacology</topic><topic>VIP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alleaume, Céline</creatorcontrib><creatorcontrib>Eychène, Alain</creatorcontrib><creatorcontrib>Harnois, Thomas</creatorcontrib><creatorcontrib>Bourmeyster, Nicolas</creatorcontrib><creatorcontrib>Constantin, Bruno</creatorcontrib><creatorcontrib>Caigneaux, Evelyne</creatorcontrib><creatorcontrib>Muller, Jean-Marc</creatorcontrib><creatorcontrib>Philippe, Michel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alleaume, Céline</au><au>Eychène, Alain</au><au>Harnois, Thomas</au><au>Bourmeyster, Nicolas</au><au>Constantin, Bruno</au><au>Caigneaux, Evelyne</au><au>Muller, Jean-Marc</au><au>Philippe, Michel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vasoactive intestinal peptide-induced neurite remodeling in human neuroblastoma SH-SY5Y cells implicates the Cdc42 GTPase and is independent of Ras-ERK pathway</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>2004-10-01</date><risdate>2004</risdate><volume>299</volume><issue>2</issue><spage>511</spage><epage>524</epage><pages>511-524</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Vasoactive intestinal peptide (VIP) is known to regulate proliferation or differentiation in normal and tumoral cells. SH-SY5Y is a differentiated cell subclone derived from the SK-N-SH human neuroblastoma cell line and possess all the components for an autocrine action of VIP. In the present study, we investigated the morphological changes and intracellular signaling pathways occurring upon VIP treatment of SH-SY5Y cells. VIP induced an early remodeling of cell projections: a branched neurite network spread out and prominent varicosities developed along neurites. Although activated by VIP, the Ras/ERK pathway was not required for the remodeling process. In contrast, pull-down experiments revealed a strong Cdc42 activation by VIP while expression of a dominant-negative Cdc42 prevented the VIP-induced neurite changes, suggesting an important role for this small GTPase in the process. 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| subjects | Cancer Cdc42 cdc42 GTP-Binding Protein - metabolism Cellular Biology ERK Genes, Dominant Humans Life Sciences Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases - metabolism Neurite remodeling Neurites - drug effects Neurites - metabolism Neuroblastoma - metabolism Neuroblastoma - pathology Neuroprotective Agents - pharmacology rac1 GTP-Binding Protein - metabolism Ras ras Proteins - metabolism SH-SY5Y neuroblastoma cells Signal Transduction Subcellular Processes Tumor Cells, Cultured Vasoactive Intestinal Peptide - pharmacology VIP |
| title | Vasoactive intestinal peptide-induced neurite remodeling in human neuroblastoma SH-SY5Y cells implicates the Cdc42 GTPase and is independent of Ras-ERK pathway |
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