Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies
Plastids are semiautonomous organelles restricted to plants and protists. These plastids are surrounded by a double membrane system, or envelope. These envelope membranes contain machineries to import nuclear-encoded proteins, and transporters for ions or metabolites, but are also essential for a ra...
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| creator | Salvi, Daniel Moyet, Lucas Seigneurin-Berny, Daphné Ferro, Myriam Joyard, Jacques Rolland, Norbert |
| description | Plastids are semiautonomous organelles restricted to plants and protists. These plastids are surrounded by a double membrane system, or envelope. These envelope membranes contain machineries to import nuclear-encoded proteins, and transporters for ions or metabolites, but are also essential for a range of plastid-specific metabolisms. Targeted semiquantitative proteomic investigations have revealed specific cross-contaminations by other cell or plastid compartments that may occur during chloroplast envelope purification. This article describes procedures developed to recover highly purified envelope fractions starting from Percoll-purified Arabidopsis chloroplasts, gives an overview of possible cross-contaminations, provides some tricks to limit these cross-contaminations, and lists immunological markers and methods that can be used to assess the purity of the envelope fractions. |
| doi_str_mv | 10.1007/978-1-61779-237-3_10 |
| format | Book Chapter |
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This article describes procedures developed to recover highly purified envelope fractions starting from Percoll-purified Arabidopsis chloroplasts, gives an overview of possible cross-contaminations, provides some tricks to limit these cross-contaminations, and lists immunological markers and methods that can be used to assess the purity of the envelope fractions.</description><identifier>ISSN: 1064-3745</identifier><identifier>ISBN: 9781617792366</identifier><identifier>ISBN: 1617792365</identifier><identifier>EISSN: 1940-6029</identifier><identifier>EISBN: 9781617792373</identifier><identifier>EISBN: 1617792373</identifier><identifier>DOI: 10.1007/978-1-61779-237-3_10</identifier><identifier>PMID: 21863444</identifier><language>eng</language><publisher>Totowa, NJ: Humana Press</publisher><subject>Arabidopsis - cytology ; Biomarkers - metabolism ; Blotting, Western ; Botanics ; Cell Fractionation - methods ; Chemical Fractionation ; Chloroplast ; Chloroplast envelope ; Chloroplasts - chemistry ; Chloroplasts - metabolism ; Cross-contamination ; Electrophoresis, Polyacrylamide Gel ; Intracellular Membranes - chemistry ; Intracellular Membranes - metabolism ; Life Sciences ; Mass Spectrometry ; Plant Leaves - cytology ; Povidone - chemistry ; Proteome ; Proteomics - methods ; Silicon Dioxide - chemistry ; Stroma ; Thylakoids ; Vegetal Biology</subject><ispartof>Chloroplast Research in Arabidopsis, 2011, Vol.775, p.189-206</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-77e87c772689bdada5ceb289fa68b361d71fdecb0fec3972b907a312d3d1e843</citedby><orcidid>0000-0003-1959-6636 ; 0000-0002-7436-9885</orcidid><relation>Methods in Molecular Biology</relation></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/978-1-61779-237-3_10$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/978-1-61779-237-3_10$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,777,778,782,791,883,27908,38238,41425,42494</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21863444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02810049$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Jarvis, R. Paul</contributor><creatorcontrib>Salvi, Daniel</creatorcontrib><creatorcontrib>Moyet, Lucas</creatorcontrib><creatorcontrib>Seigneurin-Berny, Daphné</creatorcontrib><creatorcontrib>Ferro, Myriam</creatorcontrib><creatorcontrib>Joyard, Jacques</creatorcontrib><creatorcontrib>Rolland, Norbert</creatorcontrib><title>Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies</title><title>Chloroplast Research in Arabidopsis</title><addtitle>Methods Mol Biol</addtitle><description>Plastids are semiautonomous organelles restricted to plants and protists. These plastids are surrounded by a double membrane system, or envelope. These envelope membranes contain machineries to import nuclear-encoded proteins, and transporters for ions or metabolites, but are also essential for a range of plastid-specific metabolisms. Targeted semiquantitative proteomic investigations have revealed specific cross-contaminations by other cell or plastid compartments that may occur during chloroplast envelope purification. This article describes procedures developed to recover highly purified envelope fractions starting from Percoll-purified Arabidopsis chloroplasts, gives an overview of possible cross-contaminations, provides some tricks to limit these cross-contaminations, and lists immunological markers and methods that can be used to assess the purity of the envelope fractions.</description><subject>Arabidopsis - cytology</subject><subject>Biomarkers - metabolism</subject><subject>Blotting, Western</subject><subject>Botanics</subject><subject>Cell Fractionation - methods</subject><subject>Chemical Fractionation</subject><subject>Chloroplast</subject><subject>Chloroplast envelope</subject><subject>Chloroplasts - chemistry</subject><subject>Chloroplasts - metabolism</subject><subject>Cross-contamination</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Intracellular Membranes - chemistry</subject><subject>Intracellular Membranes - metabolism</subject><subject>Life Sciences</subject><subject>Mass Spectrometry</subject><subject>Plant Leaves - cytology</subject><subject>Povidone - chemistry</subject><subject>Proteome</subject><subject>Proteomics - methods</subject><subject>Silicon Dioxide - chemistry</subject><subject>Stroma</subject><subject>Thylakoids</subject><subject>Vegetal Biology</subject><issn>1064-3745</issn><issn>1940-6029</issn><isbn>9781617792366</isbn><isbn>1617792365</isbn><isbn>9781617792373</isbn><isbn>1617792373</isbn><fulltext>true</fulltext><rsrctype>book_chapter</rsrctype><creationdate>2011</creationdate><recordtype>book_chapter</recordtype><sourceid>EIF</sourceid><recordid>eNpVkV1LHDEUhtMvqrX-g1JyV3oRm6_Nx-WyaC2sKNT7kEzOdKedmYzJrOC_N-NawasD7_tw4JwHoS-MnjFK9Q-rDWFEMa0t4UIT4Rh9g05rzJ7Cmom36JhZSYmi3L571Sn1vnZUSSK0XB2hT6X8pVRqweVHdMSZUUJKeYzubjJMPvu5SyNOLT4f76FPE-ArGEL2I-CL7JulLbjNacDr7EMX01S6gje7PuU09b7MtU0Z3-Q0Qxq6Bq9H3z8sjB8jvp53kPHveR87KJ_Rh9b3BU6f5wm6vTi_3VyS7fXPX5v1ljSSyploDUY3WnNlbIg--lUDgRvbemWCUCxq1kZoAm2hEVbzYKn2gvEoIgMjxQn6fli7872bcjf4_OCS79zleuuWjHJT_yztPavstwM75XS3hzK7oSsN9H29P-2LM2bFrTHSVPLrM7kPA8SXxf8fWgF-AEqtxj-QXUjpX6nu3GLVVUWOuSdJrhp0i1XxCFd8jrg</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Salvi, Daniel</creator><creator>Moyet, Lucas</creator><creator>Seigneurin-Berny, Daphné</creator><creator>Ferro, Myriam</creator><creator>Joyard, Jacques</creator><creator>Rolland, Norbert</creator><general>Humana Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-1959-6636</orcidid><orcidid>https://orcid.org/0000-0002-7436-9885</orcidid></search><sort><creationdate>2011</creationdate><title>Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies</title><author>Salvi, Daniel ; Moyet, Lucas ; Seigneurin-Berny, Daphné ; Ferro, Myriam ; Joyard, Jacques ; Rolland, Norbert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-77e87c772689bdada5ceb289fa68b361d71fdecb0fec3972b907a312d3d1e843</frbrgroupid><rsrctype>book_chapters</rsrctype><prefilter>book_chapters</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Arabidopsis - cytology</topic><topic>Biomarkers - metabolism</topic><topic>Blotting, Western</topic><topic>Botanics</topic><topic>Cell Fractionation - methods</topic><topic>Chemical Fractionation</topic><topic>Chloroplast</topic><topic>Chloroplast envelope</topic><topic>Chloroplasts - chemistry</topic><topic>Chloroplasts - metabolism</topic><topic>Cross-contamination</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Intracellular Membranes - chemistry</topic><topic>Intracellular Membranes - metabolism</topic><topic>Life Sciences</topic><topic>Mass Spectrometry</topic><topic>Plant Leaves - cytology</topic><topic>Povidone - chemistry</topic><topic>Proteome</topic><topic>Proteomics - methods</topic><topic>Silicon Dioxide - chemistry</topic><topic>Stroma</topic><topic>Thylakoids</topic><topic>Vegetal Biology</topic><toplevel>online_resources</toplevel><creatorcontrib>Salvi, Daniel</creatorcontrib><creatorcontrib>Moyet, Lucas</creatorcontrib><creatorcontrib>Seigneurin-Berny, Daphné</creatorcontrib><creatorcontrib>Ferro, Myriam</creatorcontrib><creatorcontrib>Joyard, Jacques</creatorcontrib><creatorcontrib>Rolland, Norbert</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salvi, Daniel</au><au>Moyet, Lucas</au><au>Seigneurin-Berny, Daphné</au><au>Ferro, Myriam</au><au>Joyard, Jacques</au><au>Rolland, Norbert</au><au>Jarvis, R. Paul</au><format>book</format><genre>bookitem</genre><ristype>CHAP</ristype><atitle>Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies</atitle><btitle>Chloroplast Research in Arabidopsis</btitle><addtitle>Methods Mol Biol</addtitle><seriestitle>Methods in Molecular Biology</seriestitle><date>2011</date><risdate>2011</risdate><volume>775</volume><spage>189</spage><epage>206</epage><pages>189-206</pages><issn>1064-3745</issn><eissn>1940-6029</eissn><isbn>9781617792366</isbn><isbn>1617792365</isbn><eisbn>9781617792373</eisbn><eisbn>1617792373</eisbn><abstract>Plastids are semiautonomous organelles restricted to plants and protists. These plastids are surrounded by a double membrane system, or envelope. These envelope membranes contain machineries to import nuclear-encoded proteins, and transporters for ions or metabolites, but are also essential for a range of plastid-specific metabolisms. Targeted semiquantitative proteomic investigations have revealed specific cross-contaminations by other cell or plastid compartments that may occur during chloroplast envelope purification. This article describes procedures developed to recover highly purified envelope fractions starting from Percoll-purified Arabidopsis chloroplasts, gives an overview of possible cross-contaminations, provides some tricks to limit these cross-contaminations, and lists immunological markers and methods that can be used to assess the purity of the envelope fractions.</abstract><cop>Totowa, NJ</cop><pub>Humana Press</pub><pmid>21863444</pmid><doi>10.1007/978-1-61779-237-3_10</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0003-1959-6636</orcidid><orcidid>https://orcid.org/0000-0002-7436-9885</orcidid></addata></record> |
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| identifier | ISSN: 1064-3745 |
| ispartof | Chloroplast Research in Arabidopsis, 2011, Vol.775, p.189-206 |
| issn | 1064-3745 1940-6029 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02810049v1 |
| source | MEDLINE; Springer Books |
| subjects | Arabidopsis - cytology Biomarkers - metabolism Blotting, Western Botanics Cell Fractionation - methods Chemical Fractionation Chloroplast Chloroplast envelope Chloroplasts - chemistry Chloroplasts - metabolism Cross-contamination Electrophoresis, Polyacrylamide Gel Intracellular Membranes - chemistry Intracellular Membranes - metabolism Life Sciences Mass Spectrometry Plant Leaves - cytology Povidone - chemistry Proteome Proteomics - methods Silicon Dioxide - chemistry Stroma Thylakoids Vegetal Biology |
| title | Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies |
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