GC and HPLC of grape monoterpenyl glycosides

The aim of the present study was to find a method alternative to enzymic hydrolysis for the analysis of grape terpenyl glycosides. GC after trimethylsilane derivatisation, and HPLC on four different stationary phases, ie bare silica, aminopropyl-, diol- and octadecyl-bonded silicas, were in turn considered for the separation of these aroma precursors extracted from grape juice, and specific enzymic hydrolyses with highly purified glycosidases showed which were the major components. These investigations show that reversed-phase liquid chromatography is better suited for both the direct and individual analysis of these glycosides.