Electrochemical proton gradient of Brevibacterium linens and its relationship to phenylalanine transport
The proton motive force generated by Brevibacterium linens was determined by the accumulation of radiolabelled tetraphenylphosphonium for transmembrane potential (Δ) and by the accumulation of benzoate ions for the H + chemical gradient (ΔpH). In resting cells at pH 8.0, the Δ was 172±10 mV while Δp...
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| Veröffentlicht in: | Annales de l'Institut Pasteur. Microbiology 1984-07, Vol.135 (1), p.91-99 |
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| creator | Boyaval, P. Moreira, E. Desmazeaud, M.J. |
| description | The proton motive force generated by
Brevibacterium linens was determined by the accumulation of radiolabelled tetraphenylphosphonium for transmembrane potential (Δ) and by the accumulation of benzoate ions for the H
+ chemical gradient (ΔpH). In resting cells at pH 8.0, the Δ was 172±10 mV while ΔpH was 9 mV. The addition of valinomycin to
B. linens in the presence of 100 mM exogenous potassium reduced, the Δ and led to a drastic inhibition of phenylalanine transport.
These findings are consistent with the hypothesis that a membrane potential is essential for active phenylalanine transport in
B. linens cells at pH 8.0. Phenylalanine transport in these cells (halotolerant strain) did not depend on the presence of Na
+.
La force proton-motrice générée par
Brevibacterium, linens a été déterminée grâce à l'accumulation du tétraphénylphosphonium marqué pour le potentiel transmembranaire, (Δ) et grâce à celle des ions benzoate pour le gradient chimique en H
+ (ΔpH). Chez les cellules au repos et à pH 8.0, le Δ est de 172±10 mV, alors que le ΔpH est de 9 mV. En présence du potassium exogène 100 mM, l'adjonction de valinomycine réduit le Δ et inhibe de manière spectaculaire le transport de la phénylalanine.
Ces résultats sont cohérents avec l'hypothèse selon laquelle un potentiel membranaire est requis pour le transport actif de la phénylalanine chez
B. linens à pH 8.0. Le transport de la phénylalanine dans ces cellules (souche halotolérante) ne dépend pas de la présence de Na
+. |
| doi_str_mv | 10.1016/S0769-2609(84)80046-0 |
| format | Article |
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Brevibacterium linens was determined by the accumulation of radiolabelled tetraphenylphosphonium for transmembrane potential (Δ) and by the accumulation of benzoate ions for the H
+ chemical gradient (ΔpH). In resting cells at pH 8.0, the Δ was 172±10 mV while ΔpH was 9 mV. The addition of valinomycin to
B. linens in the presence of 100 mM exogenous potassium reduced, the Δ and led to a drastic inhibition of phenylalanine transport.
These findings are consistent with the hypothesis that a membrane potential is essential for active phenylalanine transport in
B. linens cells at pH 8.0. Phenylalanine transport in these cells (halotolerant strain) did not depend on the presence of Na
+.
La force proton-motrice générée par
Brevibacterium, linens a été déterminée grâce à l'accumulation du tétraphénylphosphonium marqué pour le potentiel transmembranaire, (Δ) et grâce à celle des ions benzoate pour le gradient chimique en H
+ (ΔpH). Chez les cellules au repos et à pH 8.0, le Δ est de 172±10 mV, alors que le ΔpH est de 9 mV. En présence du potassium exogène 100 mM, l'adjonction de valinomycine réduit le Δ et inhibe de manière spectaculaire le transport de la phénylalanine.
Ces résultats sont cohérents avec l'hypothèse selon laquelle un potentiel membranaire est requis pour le transport actif de la phénylalanine chez
B. linens à pH 8.0. Le transport de la phénylalanine dans ces cellules (souche halotolérante) ne dépend pas de la présence de Na
+.</description><identifier>ISSN: 0769-2609</identifier><identifier>ISSN: 0300-5410</identifier><identifier>EISSN: 1878-2094</identifier><identifier>DOI: 10.1016/S0769-2609(84)80046-0</identifier><identifier>PMID: 6095716</identifier><language>eng</language><publisher>Paris: Elsevier B.V</publisher><subject>BACTERIA ; BACTERIE ; BACTERIOLOGIA ; BACTERIOLOGIE ; BACTERIOLOGY ; Biological and medical sciences ; Biological Transport, Active - drug effects ; Brevibacterium - metabolism ; Brevibacterium linens ; CHEESES ; Energy Metabolism ; Force proton-motrice ; FROMAGE ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Kinetics ; Life Sciences ; Lithium - pharmacology ; Membrane potential ; Membrane Potentials - drug effects ; Microbiology ; Permeability, membrane transport, intracellular transport ; Phenylalanine ; Phenylalanine - metabolism ; Phénylalanine ; Potassium - pharmacology ; Potentiel de membrane ; Proton gradient ; Protons ; QUESOS ; Sodium - pharmacology ; Valinomycin - pharmacology</subject><ispartof>Annales de l'Institut Pasteur. Microbiology, 1984-07, Vol.135 (1), p.91-99</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-ea9c0754565b1feb3741a105f2a7ea04a60fc2f7234ceed8056410e54f3a1863</citedby><cites>FETCH-LOGICAL-c442t-ea9c0754565b1feb3741a105f2a7ea04a60fc2f7234ceed8056410e54f3a1863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8878244$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6095716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02724059$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Boyaval, P.</creatorcontrib><creatorcontrib>Moreira, E.</creatorcontrib><creatorcontrib>Desmazeaud, M.J.</creatorcontrib><title>Electrochemical proton gradient of Brevibacterium linens and its relationship to phenylalanine transport</title><title>Annales de l'Institut Pasteur. Microbiology</title><addtitle>Ann Microbiol (Paris)</addtitle><description>The proton motive force generated by
Brevibacterium linens was determined by the accumulation of radiolabelled tetraphenylphosphonium for transmembrane potential (Δ) and by the accumulation of benzoate ions for the H
+ chemical gradient (ΔpH). In resting cells at pH 8.0, the Δ was 172±10 mV while ΔpH was 9 mV. The addition of valinomycin to
B. linens in the presence of 100 mM exogenous potassium reduced, the Δ and led to a drastic inhibition of phenylalanine transport.
These findings are consistent with the hypothesis that a membrane potential is essential for active phenylalanine transport in
B. linens cells at pH 8.0. Phenylalanine transport in these cells (halotolerant strain) did not depend on the presence of Na
+.
La force proton-motrice générée par
Brevibacterium, linens a été déterminée grâce à l'accumulation du tétraphénylphosphonium marqué pour le potentiel transmembranaire, (Δ) et grâce à celle des ions benzoate pour le gradient chimique en H
+ (ΔpH). Chez les cellules au repos et à pH 8.0, le Δ est de 172±10 mV, alors que le ΔpH est de 9 mV. En présence du potassium exogène 100 mM, l'adjonction de valinomycine réduit le Δ et inhibe de manière spectaculaire le transport de la phénylalanine.
Ces résultats sont cohérents avec l'hypothèse selon laquelle un potentiel membranaire est requis pour le transport actif de la phénylalanine chez
B. linens à pH 8.0. Le transport de la phénylalanine dans ces cellules (souche halotolérante) ne dépend pas de la présence de Na
+.</description><subject>BACTERIA</subject><subject>BACTERIE</subject><subject>BACTERIOLOGIA</subject><subject>BACTERIOLOGIE</subject><subject>BACTERIOLOGY</subject><subject>Biological and medical sciences</subject><subject>Biological Transport, Active - drug effects</subject><subject>Brevibacterium - metabolism</subject><subject>Brevibacterium linens</subject><subject>CHEESES</subject><subject>Energy Metabolism</subject><subject>Force proton-motrice</subject><subject>FROMAGE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Lithium - pharmacology</subject><subject>Membrane potential</subject><subject>Membrane Potentials - drug effects</subject><subject>Microbiology</subject><subject>Permeability, membrane transport, intracellular transport</subject><subject>Phenylalanine</subject><subject>Phenylalanine - metabolism</subject><subject>Phénylalanine</subject><subject>Potassium - pharmacology</subject><subject>Potentiel de membrane</subject><subject>Proton gradient</subject><subject>Protons</subject><subject>QUESOS</subject><subject>Sodium - pharmacology</subject><subject>Valinomycin - pharmacology</subject><issn>0769-2609</issn><issn>0300-5410</issn><issn>1878-2094</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EKkPhBZAqeYEQXQRsx449q6pUA0UaiQVdsLPuODeNURIH2zNS3x5PM5otK0v3fPfH5xByxdlnznjz5RfTzboSDVt_MvLaMCabir0gK260qQRby5dkdUZekzcp_SmM4TW7IBelpDRvVqTfDOhyDK7H0TsY6BxDDhN9jNB6nDINHf0a8eB34DJGvx_p4CecEoWppT4nGnGA7MOUej_THOjc4_Q0wABT4WiOMKU5xPyWvOpgSPju9F6Sh2-bh7v7avvz-4-7223lpBS5Qlg7ppVUjdrxDne1lhw4U50AjcAkNKxzotOilg6xNUw1kjNUsquBm6a-JNfL2B4GO0c_QnyyAby9v93aY40JLSRT6wMv7MeFLX_-u8eU7eiTw6GcjmGfrFaG6-JSAdUCuhhSitidJ3Nmj2HY5zDs0WlrpH0Ow7LSd3VasN-N2J67Tu4X_cNJh1S874pXzqczZkqSQsqCvV-wDoKFx1iQ3xujatHo446bRcRi6sFjtMmV4By2PpZkbRv8f678B5tXrzI</recordid><startdate>198407</startdate><enddate>198407</enddate><creator>Boyaval, P.</creator><creator>Moreira, E.</creator><creator>Desmazeaud, M.J.</creator><general>Elsevier B.V</general><general>Masson</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>198407</creationdate><title>Electrochemical proton gradient of Brevibacterium linens and its relationship to phenylalanine transport</title><author>Boyaval, P. ; Moreira, E. ; Desmazeaud, M.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-ea9c0754565b1feb3741a105f2a7ea04a60fc2f7234ceed8056410e54f3a1863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>BACTERIA</topic><topic>BACTERIE</topic><topic>BACTERIOLOGIA</topic><topic>BACTERIOLOGIE</topic><topic>BACTERIOLOGY</topic><topic>Biological and medical sciences</topic><topic>Biological Transport, Active - drug effects</topic><topic>Brevibacterium - metabolism</topic><topic>Brevibacterium linens</topic><topic>CHEESES</topic><topic>Energy Metabolism</topic><topic>Force proton-motrice</topic><topic>FROMAGE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Lithium - pharmacology</topic><topic>Membrane potential</topic><topic>Membrane Potentials - drug effects</topic><topic>Microbiology</topic><topic>Permeability, membrane transport, intracellular transport</topic><topic>Phenylalanine</topic><topic>Phenylalanine - metabolism</topic><topic>Phénylalanine</topic><topic>Potassium - pharmacology</topic><topic>Potentiel de membrane</topic><topic>Proton gradient</topic><topic>Protons</topic><topic>QUESOS</topic><topic>Sodium - pharmacology</topic><topic>Valinomycin - pharmacology</topic><toplevel>online_resources</toplevel><creatorcontrib>Boyaval, P.</creatorcontrib><creatorcontrib>Moreira, E.</creatorcontrib><creatorcontrib>Desmazeaud, M.J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Annales de l'Institut Pasteur. Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boyaval, P.</au><au>Moreira, E.</au><au>Desmazeaud, M.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical proton gradient of Brevibacterium linens and its relationship to phenylalanine transport</atitle><jtitle>Annales de l'Institut Pasteur. Microbiology</jtitle><addtitle>Ann Microbiol (Paris)</addtitle><date>1984-07</date><risdate>1984</risdate><volume>135</volume><issue>1</issue><spage>91</spage><epage>99</epage><pages>91-99</pages><issn>0769-2609</issn><issn>0300-5410</issn><eissn>1878-2094</eissn><abstract>The proton motive force generated by
Brevibacterium linens was determined by the accumulation of radiolabelled tetraphenylphosphonium for transmembrane potential (Δ) and by the accumulation of benzoate ions for the H
+ chemical gradient (ΔpH). In resting cells at pH 8.0, the Δ was 172±10 mV while ΔpH was 9 mV. The addition of valinomycin to
B. linens in the presence of 100 mM exogenous potassium reduced, the Δ and led to a drastic inhibition of phenylalanine transport.
These findings are consistent with the hypothesis that a membrane potential is essential for active phenylalanine transport in
B. linens cells at pH 8.0. Phenylalanine transport in these cells (halotolerant strain) did not depend on the presence of Na
+.
La force proton-motrice générée par
Brevibacterium, linens a été déterminée grâce à l'accumulation du tétraphénylphosphonium marqué pour le potentiel transmembranaire, (Δ) et grâce à celle des ions benzoate pour le gradient chimique en H
+ (ΔpH). Chez les cellules au repos et à pH 8.0, le Δ est de 172±10 mV, alors que le ΔpH est de 9 mV. En présence du potassium exogène 100 mM, l'adjonction de valinomycine réduit le Δ et inhibe de manière spectaculaire le transport de la phénylalanine.
Ces résultats sont cohérents avec l'hypothèse selon laquelle un potentiel membranaire est requis pour le transport actif de la phénylalanine chez
B. linens à pH 8.0. Le transport de la phénylalanine dans ces cellules (souche halotolérante) ne dépend pas de la présence de Na
+.</abstract><cop>Paris</cop><pub>Elsevier B.V</pub><pmid>6095716</pmid><doi>10.1016/S0769-2609(84)80046-0</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0769-2609 |
| ispartof | Annales de l'Institut Pasteur. Microbiology, 1984-07, Vol.135 (1), p.91-99 |
| issn | 0769-2609 0300-5410 1878-2094 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02724059v1 |
| source | MEDLINE; Gallica Periodicals; Alma/SFX Local Collection |
| subjects | BACTERIA BACTERIE BACTERIOLOGIA BACTERIOLOGIE BACTERIOLOGY Biological and medical sciences Biological Transport, Active - drug effects Brevibacterium - metabolism Brevibacterium linens CHEESES Energy Metabolism Force proton-motrice FROMAGE Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Kinetics Life Sciences Lithium - pharmacology Membrane potential Membrane Potentials - drug effects Microbiology Permeability, membrane transport, intracellular transport Phenylalanine Phenylalanine - metabolism Phénylalanine Potassium - pharmacology Potentiel de membrane Proton gradient Protons QUESOS Sodium - pharmacology Valinomycin - pharmacology |
| title | Electrochemical proton gradient of Brevibacterium linens and its relationship to phenylalanine transport |
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